(Linear) (Six-base) MAPSORT of: KIEE3684.seq Check: 7725 from: 1 to: 1467 KIEE3684.orf With 182 enzymes: SgfI * March 22, 2012 16:39 .. Acc65I G'GTAC_C Cuts at: 0 1397 1467 Size: 1397 70 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 568 1467 Size: 325 243 899 Fragments arranged by size: 899 325 243 AgeI A'CCGG_T Cuts at: 0 1394 1467 Size: 1394 73 AleI CACnn'nnGTG Cuts at: 0 1024 1467 Size: 1024 443 AlwNI CAG_nnn'CTG Cuts at: 0 1047 1467 Size: 1047 420 ApaI G_GGCC'C Cuts at: 0 334 684 1467 Size: 334 350 783 Fragments arranged by size: 783 350 334 ApoI r'AATT_y Cuts at: 0 933 1467 Size: 933 534 AvaI C'yCGr_G Cuts at: 0 713 770 920 1467 Size: 713 57 150 547 Fragments arranged by size: 713 547 150 57 BamHI G'GATC_C Cuts at: 0 166 1467 Size: 166 1301 BanI G'GyrC_C Cuts at: 0 86 1397 1467 Size: 86 1311 70 Fragments arranged by size: 1311 86 70 BanII G_rGCy'C Cuts at: 0 73 295 334 478 499 684 1467 Size: 73 222 39 144 21 185 783 Fragments arranged by size: 783 222 185 144 73 39 21 BbeI G_GCGC'C Cuts at: 0 90 1467 Size: 90 1377 BbvCI CC'TCA_GC Cuts at: 0 949 1467 Size: 949 518 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 357 391 1467 Size: 357 34 1076 Fragments arranged by size: 1076 357 34 BciVI GTATCCnnnnn_n' Cuts at: 0 812 1467 Size: 812 655 BglI GCCn_nnn'nGGC Cuts at: 0 1417 1467 Size: 1417 50 Bme1580I G_kGCm'C Cuts at: 0 334 684 1467 Size: 334 350 783 Fragments arranged by size: 783 350 334 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 309 1467 Size: 309 1158 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 383 1225 1422 1467 Size: 383 842 197 45 Fragments arranged by size: 842 383 197 45 Bpu10I CC'TnA_GC Cuts at: 0 67 121 949 1467 Size: 67 54 828 518 Fragments arranged by size: 828 518 67 54 BsaI GGTCTCn'nnnn_ Cuts at: 0 335 1467 Size: 335 1132 BsaHI Gr'CG_yC Cuts at: 0 87 1376 1467 Size: 87 1289 91 Fragments arranged by size: 1289 91 87 BsaWI w'CCGG_w Cuts at: 0 1394 1467 Size: 1394 73 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 880 910 1243 1273 1467 Size: 880 30 333 30 194 Fragments arranged by size: 880 333 194 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 205 208 211 220 229 244 327 607 Size: 205 3 3 9 9 15 83 280 Cuts at: 607 610 613 688 938 1467 Size: 3 3 75 250 529 Fragments arranged by size: 529 280 250 205 83 75 15 9 9 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 477 498 1077 1467 Size: 477 21 579 390 Fragments arranged by size: 579 477 390 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 802 1207 1467 Size: 802 405 260 Fragments arranged by size: 802 405 260 BsiEI CG_ry'CG Cuts at: 0 1362 1467 Size: 1362 105 BsiHKAI G_wGCw'C Cuts at: 0 295 728 1467 Size: 295 433 739 Fragments arranged by size: 739 433 295 BsmBI CGTCTCn'nnnn_ Cuts at: 0 692 1467 Size: 692 775 Bsp1286I G_dGCh'C Cuts at: 0 73 295 334 478 499 684 728 1467 Size: 73 222 39 144 21 185 44 739 Fragments arranged by size: 739 222 185 144 73 44 39 21 BsrFI r'CCGG_y Cuts at: 0 761 1394 1467 Size: 761 633 73 Fragments arranged by size: 761 633 73 BstEII G'GTnAC_C Cuts at: 0 1287 1467 Size: 1287 180 BstXI CCAn_nnnn'nTGG Cuts at: 0 513 963 1077 1241 1467 Size: 513 450 114 164 226 Fragments arranged by size: 513 450 226 164 114 BstYI r'GATC_y Cuts at: 0 166 1467 Size: 166 1301 Bsu36I CC'TnA_GG Cuts at: 0 468 1467 Size: 468 999 BtgI C'CryG_G Cuts at: 0 82 161 695 1086 1467 Size: 82 79 534 391 381 Fragments arranged by size: 534 391 381 82 79 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1413 1467 Size: 1413 54 DraIII CAC_nnn'GTG Cuts at: 0 1009 1467 Size: 1009 458 EaeI y'GGCC_r Cuts at: 0 1359 1467 Size: 1359 108 EagI C'GGCC_G Cuts at: 0 1359 1467 Size: 1359 108 EarI CTCTTCn'nnn_ Cuts at: 0 124 197 221 283 487 584 1467 Size: 124 73 24 62 204 97 883 Fragments arranged by size: 883 204 124 97 73 62 24 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1384 1467 Size: 1384 83 EcoICRI GAG'CTC Cuts at: 0 293 1467 Size: 293 1174 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 325 383 568 1225 1422 1467 Size: 325 58 185 657 197 45 Fragments arranged by size: 657 325 197 185 58 45 EcoNI CCTnn'n_nnAGG Cuts at: 0 25 1039 1467 Size: 25 1014 428 Fragments arranged by size: 1014 428 25 EcoO109I rG'GnC_Cy Cuts at: 0 78 141 330 462 680 681 1143 1467 Size: 78 63 189 132 218 1 462 324 Fragments arranged by size: 462 324 218 189 132 78 63 1 HaeII r_GCGC'y Cuts at: 0 90 1467 Size: 90 1377 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1243 1275 1467 Size: 1243 32 192 Fragments arranged by size: 1243 192 32 KasI G'GCGC_C Cuts at: 0 86 1467 Size: 86 1381 KpnI G_GTAC'C Cuts at: 0 1401 1467 Size: 1401 66 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 980 1467 Size: 980 487 MslI CAynn'nnrTG Cuts at: 0 1024 1328 1467 Size: 1024 304 139 Fragments arranged by size: 1024 304 139 MspA1I CmG'CkG Cuts at: 0 84 1054 1404 1467 Size: 84 970 350 63 Fragments arranged by size: 970 350 84 63 NaeI GCC'GGC Cuts at: 0 763 1467 Size: 763 704 NarI GG'CG_CC Cuts at: 0 87 1467 Size: 87 1380 NgoMIV G'CCGG_C Cuts at: 0 761 1467 Size: 761 706 NspI r_CATG'y Cuts at: 0 801 1467 Size: 801 666 PasI CC'CwG_GG Cuts at: 0 327 741 1467 Size: 327 414 726 Fragments arranged by size: 726 414 327 PflMI CCAn_nnn'nTGG Cuts at: 0 1009 1467 Size: 1009 458 PpuMI rG'GwC_Cy Cuts at: 0 462 1143 1467 Size: 462 681 324 Fragments arranged by size: 681 462 324 PspOMI G'GGCC_C Cuts at: 0 330 680 1467 Size: 330 350 787 Fragments arranged by size: 787 350 330 PstI C_TGCA'G Cuts at: 0 1355 1418 1467 Size: 1355 63 49 Fragments arranged by size: 1355 63 49 PvuII CAG'CTG Cuts at: 0 1054 1467 Size: 1054 413 SacI G_AGCT'C Cuts at: 0 295 1467 Size: 295 1172 SacII CC_GC'GG Cuts at: 0 85 1467 Size: 85 1382 SanDI GG'GwC_CC Cuts at: 0 1143 1467 Size: 1143 324 SapI GCTCTTCn'nnn_ Cuts at: 0 283 487 1467 Size: 283 204 980 Fragments arranged by size: 980 283 204 SbfI CC_TGCA'GG Cuts at: 0 1418 1467 Size: 1418 49 SfcI C'TryA_G Cuts at: 0 1351 1414 1467 Size: 1351 63 53 Fragments arranged by size: 1351 63 53 SfoI GGC'GCC Cuts at: 0 88 1467 Size: 88 1379 SmaI CCC'GGG Cuts at: 0 715 772 1467 Size: 715 57 695 Fragments arranged by size: 715 695 57 StuI AGG'CCT Cuts at: 0 1297 1420 1467 Size: 1297 123 47 Fragments arranged by size: 1297 123 47 StyI C'CwwG_G Cuts at: 0 320 334 1278 1467 Size: 320 14 944 189 Fragments arranged by size: 944 320 189 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1306 1467 Size: 1306 161 XmaI C'CCGG_G Cuts at: 0 713 770 1467 Size: 713 57 697 Fragments arranged by size: 713 697 57 XmnI GAAnn'nnTTC Cuts at: 0 841 973 1467 Size: 841 132 494 Fragments arranged by size: 841 494 132 Enzymes that do cut: Acc65I AcuI AgeI AleI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI BglI Bme1580I BplI BpmI Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MslI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI SfcI SfoI SmaI StuI StyI TaqII XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtsI ClaI CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI MscI NcoI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI TstI Tth111I XbaI XcmI XhoI ZraI