################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccII AccIII AclI AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AvrII BbrPI BcgI BciVI BclI BcnI BglI BglII BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT22I FauI FbaI FseI HapII HpaII KspI MamI MluI MroI MspI MvnI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### Acc65I 1136 GGTACC G^GTACC AciI 1184 GCGG CCGC(-3/-1) AcyI 1554 GGCGCC G[GA]^CG[CT]C AflII 745 CTTAAG C^TTAAG Asp718I 1136 GGTACC G^GTACC AviII 1076 TGCGCA TGC^GCA BalI 1437 TGGCCA TGG^CCA BamHI 1544 GGATCC G^GATCC BbeI 1554 GGCGCC GGCGC^C BbvCI 1044 GCTGAGG CCTCAGC(-5/-2) BfrI 745 CTTAAG C^TTAAG BlpI 678 GCTGAGC GC^T[ACGT]AGC BpmI 86 CTCCAG CTGGAG(16/14) Bpu1102I 678 GCTGAGC GC^T[ACGT]AGC BsaAI 883 TACGTG [CT]AC^GT[GA] BsaHI 1554 GGCGCC G[GA]^CG[CT]C BsmI 1883 GCATTC GAATGC(1/-1) Bsp1407I 1591 TGTACA T^GTACA BspMI 1354 GCAGGT ACCTGC(4/8) BsrGI 1591 TGTACA T^GTACA BssSI 103 CTCGTG CACGAG(-5/-1) BstBI 1753 TTCGAA TT^CGAA BstYI 1544 GGATCC [GA]^GATC[CT] CelII 678 GCTGAGC GC^T[ACGT]AGC DraI 1278 TTTAAA TTT^AAA DraII 189 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 1121 CCATGG C^C[GA][CT]GG EaeI 1437 TGGCCA [CT]^GGCC[GA] EcoO109I 189 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRV 1322 GATATC GAT^ATC FspI 1076 TGCGCA TGC^GCA HaeII 1554 GGCGCC [GA]GCGC^[CT] HgaI 714 GCGTC GACGC(5/10) Hin1I 1554 GGCGCC G[GA]^CG[CT]C HindIII 466 AAGCTT A^AGCTT HpaI 643 GTTAAC GTT^AAC KasI 1554 GGCGCC G^GCGCC KpnI 1136 GGTACC GGTAC^C MfeI 1706 CAATTG C^AATTG MflI 1544 GGATCC [GA]^GATC[CT] MluNI 1437 TGGCCA TGG^CCA MscI 1437 TGGCCA TGG^CCA MspA1I 1054 CAGCTG C[AC]G^C[GT]G MunI 1706 CAATTG C^AATTG NarI 1554 GGCGCC GG^CGCC NcoI 1121 CCATGG C^CATGG NspV 1753 TTCGAA TT^CGAA PpuMI 189 AGGACCT [GA]G^G[AT]CC[CT] PshAI 1563 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1054 CAGCTG CAG^CTG SapI 802 GAAGAGC GCTCTTC(1/4) SfcI 520 CTACAG C^T[GA][CT]AG SfoI 1554 GGCGCC GGC^GCC SfuI 1753 TTCGAA TT^CGAA SspBI 1591 TGTACA T^GTACA XbaI 1939 TCTAGA T^CTAGA XhoII 1544 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AseI 831 ATTAAT AT^TAAT 877 ATTAAT AsnI 831 ATTAAT AT^TAAT 877 ATTAAT BanI 1136 GGTACC G^G[CT][GA]CC 1554 GGCGCC BseRI 1037 GAGGAG GAGGAG(10/8) 1460 GAGGAG BsgI 1958 CTGCAC GTGCAG(16/14) 1816 GTGCAG BsmFI 135 GTCCC GGGAC(10/14) 1947 GTCCC BsrDI 674 CATTGC GCAATG(2/0) 1582 CATTGC BstXI 4 CCATTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1016 CCACTCACTTGG Cac8I 943 GCCAGC GC[ACGT]^[ACGT]GC 1891 GCTTGC EarI 109 GAAGAG CTCTTC(1/4) 802 GAAGAG HincII 72 GTCAAC GT[CT]^[GA]AC 643 GTTAAC HindII 72 GTCAAC GT[CT]^[GA]AC 643 GTTAAC Ksp632I 109 GAAGAG CTCTTC(1/4) 802 GAAGAG MaeII 641 ACGT A^CGT 884 ACGT MslI 5 CATTGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1303 CACCTAAGTG NspI 1079 GCATGT [GA]CATG^[CT] 1586 GCATGT PleI 326 GAGTC GAGTC(4/5) 1945 GAGTC PshBI 831 ATTAAT AT^TAAT 877 ATTAAT SmlI 745 CTTAAG C^T[CT][GA]AG 763 CTTGAG TfiI 525 GATTC G^A[AT]TC 1870 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI Bsp1286I BspHI BsrI BssKI BstF5I BstNI CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I