(Circular) (Six-base) MAPSORT of: pFN21AE3691.seq Check: 9688 from: 1 to: 6670 pFN21AE3691 6670 bp With 182 enzymes: SgfI * March 28, 2012 12:20 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3350 3350 Size: 6670 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4480 278 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4013 1937 Size: 2076 4594 Acc65I G'GTAC_C Cuts at: 3141 3991 3141 Size: 850 5820 AclI AA'CG_TT Cuts at: 1563 4797 5170 1563 Size: 3234 373 3063 Fragments arranged by size: 3234 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3223 3622 4733 6117 6385 1632 Size: 1591 399 1111 1384 268 1917 Fragments arranged by size: 1917 1591 1384 1111 399 268 AflII C'TTAA_G Cuts at: 820 1017 2750 820 Size: 197 1733 4740 Fragments arranged by size: 4740 1733 197 AflIII A'CryG_T Cuts at: 1203 5569 6546 1203 Size: 4366 977 1327 Fragments arranged by size: 4366 1327 977 AgeI A'CCGG_T Cuts at: 5501 5501 Size: 6670 AhdI GACnn_n'nnGTC Cuts at: 5399 5399 Size: 6670 AleI CACnn'nnGTG Cuts at: 1166 3312 1166 Size: 2146 4524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6670 AlwNI CAG_nnn'CTG Cuts at: 2057 2533 3061 4413 5985 2057 Size: 476 528 1352 1572 2742 Fragments arranged by size: 2742 1572 1352 528 476 ApaLI G'TGCA_C Cuts at: 4727 5883 4727 Size: 1156 5514 ApoI r'AATT_y Cuts at: 1493 2045 2668 2932 3169 3979 4205 4357 6466 Size: 552 623 264 237 810 226 152 2109 Cuts at: 6466 1493 Size: 1697 Fragments arranged by size: 2109 1697 810 623 552 264 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 2837 2883 5224 160 Size: 872 1805 46 2341 1606 Fragments arranged by size: 2341 1805 1606 872 46 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 AvaI C'yCGr_G Cuts at: 1870 1943 3995 1870 Size: 73 2052 4545 Fragments arranged by size: 4545 2052 73 BamHI G'GATC_C Cuts at: 3549 4000 4367 3549 Size: 451 367 5852 Fragments arranged by size: 5852 451 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3141 3559 3991 5446 618 Size: 325 217 627 1354 418 432 1455 1842 Fragments arranged by size: 1842 1455 1354 627 432 418 325 217 BanII G_rGCy'C Cuts at: 1391 2030 2775 2880 3435 3519 3989 1391 Size: 639 745 105 555 84 470 4072 Fragments arranged by size: 4072 745 639 555 470 105 84 BbeI G_GCGC'C Cuts at: 3563 3563 Size: 6670 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2127 2175 3229 3748 928 Size: 971 228 48 1054 519 3850 Fragments arranged by size: 3850 1054 971 519 228 48 BbvCI CC'TCA_GC Cuts at: 3050 3050 Size: 6670 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4861 4895 1903 Size: 34 50 34 2840 34 3678 Fragments arranged by size: 3678 2840 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4562 5772 4562 Size: 1210 5460 BclI T'GATC_A Cuts at: 1548 1548 Size: 6670 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5281 136 Size: 107 122 71 631 605 3609 1525 Fragments arranged by size: 3609 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6665 6665 Size: 6670 BlpI GC'TnA_GC Cuts at: 2684 4087 2684 Size: 1403 5267 Bme1580I G_kGCm'C Cuts at: 1790 3319 4731 5887 1790 Size: 1529 1412 1156 2573 Fragments arranged by size: 2573 1529 1412 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6670 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 4401 5359 Size: 416 53 83 399 24 628 2356 958 Cuts at: 5359 442 Size: 1753 Fragments arranged by size: 2356 1753 958 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6670 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3629 3629 Size: 6670 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2076 5330 1239 Size: 837 3254 2579 Fragments arranged by size: 3254 2579 837 Bpu10I CC'TnA_GC Cuts at: 3050 3668 3050 Size: 618 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2789 4795 5660 5958 2789 Size: 2006 865 298 3501 Fragments arranged by size: 3501 2006 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 882 Size: 617 3834 2219 Fragments arranged by size: 3834 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2890 4461 493 Size: 711 1686 1571 2702 Fragments arranged by size: 2702 1686 1571 711 BsaBI GATnn'nnATC Cuts at: 6664 6664 Size: 6670 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3560 4477 4859 5494 Size: 53 83 186 936 2027 917 382 635 Cuts at: 5494 275 Size: 1451 Fragments arranged by size: 2027 1451 936 917 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5103 5501 5775 5922 6333 5103 Size: 398 274 147 411 5440 Fragments arranged by size: 5440 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2014 2044 3506 3536 1178 Size: 30 165 30 611 30 1462 30 4312 Fragments arranged by size: 4312 1462 611 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3057 3480 1186 Size: 1871 423 4376 Fragments arranged by size: 4376 1871 423 BseYI C'CCAG_C Cuts at: 5873 6369 5873 Size: 496 6174 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3842 3948 1163 Size: 573 2106 106 3885 Fragments arranged by size: 3885 2106 573 106 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4120 4881 5909 1540 Size: 275 186 2119 761 1028 2301 Fragments arranged by size: 2301 2119 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2030 2690 2775 2880 3519 4731 4816 5887 2030 Size: 660 85 105 639 1212 85 1071 2813 Fragments arranged by size: 2813 1212 1071 660 639 105 85 85 BsmI GAATG_Cn' Cuts at: 3888 4181 4274 3888 Size: 293 93 6284 Fragments arranged by size: 6284 293 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2030 2690 2775 2880 3319 3435 3519 Size: 399 240 660 85 105 439 116 84 Cuts at: 3519 3989 4731 4816 5887 1391 Size: 470 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 660 470 439 399 240 116 105 85 85 84 BspEI T'CCGG_A Cuts at: 6333 6333 Size: 6670 BspHI T'CATG_A Cuts at: 2334 2907 3496 4559 6289 2334 Size: 573 589 1063 1730 2715 Fragments arranged by size: 2715 1730 1063 589 573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3350 4025 844 Size: 2506 675 3489 Fragments arranged by size: 3489 2506 675 BsrBI CCG'CTC Cuts at: 1394 1823 4557 1394 Size: 429 2734 3507 Fragments arranged by size: 3507 2734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2678 3586 5165 5339 65 Size: 1183 1430 908 1579 174 1396 Fragments arranged by size: 1579 1430 1396 1183 908 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5314 5501 1700 Size: 110 3504 187 2869 Fragments arranged by size: 3504 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3596 96 Size: 3500 3170 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6670 BssSI C'ACGA_G Cuts at: 2108 4730 5742 2108 Size: 2622 1012 3036 Fragments arranged by size: 3036 2622 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6670 BstBI TT'CG_AA Cuts at: 3759 5474 3759 Size: 1715 4955 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6670 BstXI CCAn_nnnn'nTGG Cuts at: 2016 3028 2016 Size: 1012 5658 BstYI r'GATC_y Cuts at: 1972 3549 4000 4367 4752 4769 6221 6307 6319 Size: 1577 451 367 385 17 1452 86 12 Cuts at: 6319 6665 1972 Size: 346 1977 Fragments arranged by size: 1977 1577 1452 451 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3126 513 Size: 552 2061 4057 Fragments arranged by size: 4057 2061 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3139 487 Size: 683 56 1913 4018 Fragments arranged by size: 4018 1913 683 56 BtsI GCAGTG_nn' Cuts at: 4186 4980 5000 4186 Size: 794 20 5856 Fragments arranged by size: 5856 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6670 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6635 DraI TTT'AAA Cuts at: 3285 3974 3285 Size: 689 5981 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5677 809 Size: 507 4361 1802 Fragments arranged by size: 4361 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3442 4117 5006 Size: 54 1425 50 275 18 1612 675 889 Cuts at: 5006 6635 8 Size: 1629 43 Fragments arranged by size: 1629 1612 1425 889 675 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4117 1537 Size: 275 2305 4090 Fragments arranged by size: 4090 2305 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2109 2802 4600 1353 Size: 756 693 1798 3423 Fragments arranged by size: 3423 1798 756 693 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3204 5257 5631 5777 387 Size: 1064 138 1615 2053 374 146 1280 Fragments arranged by size: 2053 1615 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2028 2773 2878 3517 2028 Size: 745 105 639 5181 Fragments arranged by size: 5181 745 639 105 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2076 3223 3622 4733 5330 6117 6385 Size: 393 444 1147 399 1111 597 787 268 Cuts at: 6385 1239 Size: 1524 Fragments arranged by size: 1524 1147 1111 787 597 444 399 393 268 EcoO109I rG'GnC_Cy Cuts at: 2195 2195 Size: 6670 EcoRI G'AATT_C Cuts at: 3979 4357 3979 Size: 378 6292 EcoRV GAT'ATC Cuts at: 3329 3329 Size: 6670 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 3316 2291 Size: 1025 5645 FspI TGC'GCA Cuts at: 3083 5176 3083 Size: 2093 4577 HaeII r_GCGC'y Cuts at: 1678 3563 5817 1678 Size: 1885 2254 2531 Fragments arranged by size: 2531 2254 1885 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3098 3130 5311 5343 5385 Size: 32 135 32 1526 32 2181 32 42 Cuts at: 5385 5417 1373 Size: 32 2626 Fragments arranged by size: 2626 2181 1526 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2079 2650 4014 4260 669 Size: 988 281 141 571 1364 246 3079 Fragments arranged by size: 3079 1364 988 571 281 246 141 HindIII A'AGCT_T Cuts at: 748 2471 748 Size: 1723 4947 HpaI GTT'AAC Cuts at: 2650 4260 2650 Size: 1610 5060 KasI G'GCGC_C Cuts at: 3559 3559 Size: 6670 KpnI G_GTAC'C Cuts at: 3145 3995 3145 Size: 850 5820 MfeI C'AATT_G Cuts at: 3711 4269 3711 Size: 558 6112 MluI A'CGCG_T Cuts at: 6546 6546 Size: 6670 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2581 5784 5968 1305 Size: 1276 3203 184 2007 Fragments arranged by size: 3203 2007 1276 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3444 10 Size: 54 1425 343 1612 3236 Fragments arranged by size: 3236 1612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2014 3312 4628 4987 5146 518 Size: 648 848 1298 1316 359 159 2042 Fragments arranged by size: 2042 1316 1298 848 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3061 4086 4763 5911 6156 1606 Size: 1455 1025 677 1148 245 2120 Fragments arranged by size: 2120 1455 1148 1025 677 245 NarI GG'CG_CC Cuts at: 3560 3560 Size: 6670 NcoI C'CATG_G Cuts at: 513 1065 3126 513 Size: 552 2061 4057 Fragments arranged by size: 4057 2061 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6670 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6670 NotI GC'GGCC_GC Cuts at: 4117 4117 Size: 6670 NspI r_CATG'y Cuts at: 1132 1229 3088 3595 4028 5573 1132 Size: 97 1859 507 433 1545 2229 Fragments arranged by size: 2229 1859 1545 507 433 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6670 PciI A'CATG_T Cuts at: 5569 5569 Size: 6670 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6670 PmeI GTTT'AAAC Cuts at: 3974 3974 Size: 6670 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2757 2789 4739 4771 6252 6284 2757 Size: 32 1950 32 1481 32 3143 Fragments arranged by size: 3143 1950 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2195 2195 Size: 6670 PshAI GACnn'nnGTC Cuts at: 1613 3572 1613 Size: 1959 4711 PsiI TTA'TAA Cuts at: 4240 4240 Size: 6670 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2905 2937 2905 Size: 32 6638 PstI C_TGCA'G Cuts at: 830 4022 830 Size: 3192 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6670 PvuII CAG'CTG Cuts at: 3061 3061 Size: 6670 SacI G_AGCT'C Cuts at: 2030 2775 2880 3519 2030 Size: 745 105 639 5181 Fragments arranged by size: 5181 745 639 105 SalI G'TCGA_C Cuts at: 1936 4012 1936 Size: 2076 4594 SapI GCTCTTCn'nnn_ Cuts at: 2802 2802 Size: 6670 SbfI CC_TGCA'GG Cuts at: 4022 4022 Size: 6670 ScaI AGT'ACT Cuts at: 4918 6630 4918 Size: 1712 4958 SfcI C'TryA_G Cuts at: 826 1045 2525 4018 5153 5834 6025 826 Size: 219 1480 1493 1135 681 191 1471 Fragments arranged by size: 1493 1480 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3561 3561 Size: 6670 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6670 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6670 SmaI CCC'GGG Cuts at: 1872 3997 1872 Size: 2125 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2750 2768 4774 5675 5937 820 Size: 197 926 807 18 2006 901 262 1553 Fragments arranged by size: 2006 1553 926 901 807 262 197 18 SnaBI TAC'GTA Cuts at: 493 4461 493 Size: 3968 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6670 SphI G_CATG'C Cuts at: 1132 4028 1132 Size: 2896 3774 SspI AAT'ATT Cuts at: 5 52 4594 5 Size: 47 4542 2081 Fragments arranged by size: 4542 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6670 StyI C'CwwG_G Cuts at: 513 1065 2143 2187 3126 3137 3880 4109 513 Size: 552 1078 44 939 11 743 229 3074 Fragments arranged by size: 3074 1078 939 743 552 229 44 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2423 4697 4714 4867 5052 5497 325 Size: 1255 843 2274 17 153 185 445 1498 Fragments arranged by size: 2274 1498 1255 843 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2759 3596 4916 Size: 274 80 33 51 1443 782 837 1320 Cuts at: 4916 6628 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1320 837 782 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3506 3538 3898 3930 6630 6662 3506 Size: 32 360 32 2700 32 3514 Fragments arranged by size: 3514 2700 360 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6670 XbaI T'CTAG_A Cuts at: 3944 4006 3944 Size: 62 6608 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6670 XmaI C'CCGG_G Cuts at: 1870 3995 1870 Size: 2125 4545 XmnI GAAnn'nnTTC Cuts at: 4799 4799 Size: 6670 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4478 276 Size: 53 83 186 936 2944 2468 Fragments arranged by size: 2944 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI