################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AccIII AclI AgeI AhdI Aor51HI ApaI AscI AspEI AspI AvrII BbrPI BbvCI BclI BlnI BlpI Bpu1102I BsaAI BsaI BseAI BsiEI BsiWI BspDI BspEI BssHII CelII ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI HpaI MluI MroI NcoI NheI NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SapI SbfI SfiI SnaBI SpeI Sse8387I SspI StuI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AcsI 2263 GAATTC [GA]^AATT[CT] Alw44I 210 GTGCAC G^TGCAC ApaLI 210 GTGCAC G^TGCAC ApoI 2263 GAATTC [GA]^AATT[CT] Asp700I 1888 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 2961 TGCGCA TGC^GCA BamHI 833 GGATCC G^GATCC BbeI 257 GGCGCC GGCGC^C BcgI 1323 GCAGGAGTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 2671 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1840 GCATTC GAATGC(1/-1) BspHI 4323 TCATGA T^CATGA BssSI 1154 CTCGTG CACGAG(-5/-1) BstBI 2330 TTCGAA TT^CGAA BstEII 3213 GGTCACC G^GT[ACGT]ACC BstPI 3213 GGTCACC G^GT[ACGT]ACC DraI 1111 TTTAAA TTT^AAA DrdI 3674 GACTAGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 3213 GGTCACC G^GT[ACGT]ACC EcoRI 2263 GAATTC G^AATTC FspI 2961 TGCGCA TGC^GCA HgaI 727 GACGC GACGC(5/10) HincII 748 GTCAAC GT[CT]^[GA]AC HindII 748 GTCAAC GT[CT]^[GA]AC HindIII 2203 AAGCTT A^AGCTT KasI 257 GGCGCC G^GCGCC KspI 205 CCGCGG CCGC^GG MamI 2671 GATCTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 361 CAATTG C^AATTG MunI 361 CAATTG C^AATTG NaeI 3806 GCCGGC GCC^GGC NarI 257 GGCGCC GG^CGCC NdeI 2102 CATATG CA^TATG NgoMIV 3806 GCCGGC G^CCGGC NspV 2330 TTCGAA TT^CGAA PshAI 2139 GACTGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 1949 CTGCAG CTGCA^G RcaI 4323 TCATGA T^CATGA SacII 205 CCGCGG CCGC^GG ScaI 1502 AGTACT AGT^ACT SexAI 2295 ACCTGGT A^CC[AT]GGT SfoI 257 GGCGCC GGC^GCC SfuI 2330 TTCGAA TT^CGAA SgrAI 3805 CGCCGGCG C[GA]^CCGG[CT]G SphI 171 GCATGC GCATG^C XmnI 1888 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 958 GACGTC GACGT^C 1618 GACGTC AccII 206 CGCG CG^CG 4229 CGCG AflII 1834 CTTAAG C^TTAAG 3750 CTTAAG AseI 2392 ATTAAT AT^TAAT 3274 ATTAAT AsnI 2392 ATTAAT AT^TAAT 3274 ATTAAT BciVI 4130 GGATAC GTATCC(6/5) 3861 GTATCC BfrI 1834 CTTAAG C^TTAAG 3750 CTTAAG BglII 1859 AGATCT A^GATCT 2618 AGATCT BsaWI 836 TCCGGT [AT]^CCGG[AT] 1637 ACCGGA BsmBI 3501 CGTCTC CGTCTC(1/5) 3894 CGTCTC Bsp1407I 1217 TGTACA T^GTACA 3261 TGTACA BspMI 128 ACCTGC ACCTGC(4/8) 3783 ACCTGC BsrBI 1446 CCGCTC CCGCTC(-3/-3) 2383 GAGCGG BsrFI 1005 GCCGGT [GA]^CCGG[CT] 3806 GCCGGC BsrGI 1217 TGTACA T^GTACA 3261 TGTACA Bst1107I 1770 GTATAC GTA^TAC 2548 GTATAC BstAPI 1150 GCATCTCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3735 GCACCCAGTGC BstUI 206 CGCG CG^CG 4229 CGCG BstZ17I 1770 GTATAC GTA^TAC 2548 GTATAC Cfr10I 1005 GCCGGT [GA]^CCGG[CT] 3806 GCCGGC HaeII 257 GGCGCC [GA]GCGC^[CT] 413 AGCGCC MvnI 206 CGCG CG^CG 4229 CGCG PshBI 2392 ATTAAT AT^TAAT 3274 ATTAAT PvuII 3982 CAGCTG CAG^CTG 4280 CAGCTG SfcI 1949 CTGCAG C^T[GA][CT]AG 2050 CTACAG SmaI 3563 CCCGGG CCC^GGG 3646 CCCGGG SmlI 1834 CTTAAG C^T[CT][GA]AG 3750 CTTAAG SspBI 1217 TGTACA T^GTACA 3261 TGTACA XbaI 1302 TCTAGA T^CTAGA 2607 TCTAGA XcmI 2483 CCAACCGAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3294 CCACATCATCCGTGG XmaCI 3563 CCCGGG C^CCGGG 3646 CCCGGG XmaI 3563 CCCGGG C^CCGGG 3646 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### Acc65I AccI AciI AcyI AfaI AflIII AluI AlwI AlwNI AocI Asp718I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII