Restriction map for
pFN21AE9647
(Linear) (Six-base) MAPSORT of: KIEE9647.seq Check: 7178 from: 1 to: 4401
KIEE9647.orf
With 182 enzymes: SgfI *
March 22, 2012 18:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 3792 4401
Size: 3792 609
AatII G_ACGT'C
Cuts at: 0 962 1622 4401
Size: 962 660 2779
Fragments arranged by size:
2779 962 660
AccI GT'mk_AC
Cuts at: 0 1257 1771 2549 4401
Size: 1257 514 778 1852
Fragments arranged by size:
1852 1257 778 514
Acc65I G'GTAC_C
Cuts at: 0 1475 1634 2299 4401
Size: 1475 159 665 2102
Fragments arranged by size:
2102 1475 665 159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1003 1082 1109 1255 1456 1720 1879
Size: 227 776 79 27 146 201 264 159
Cuts at: 1879 2824 2994 3404 3520 4401
Size: 945 170 410 116 881
Fragments arranged by size:
945 881 776 410 264 227 201 170
159 146 116 79 27
AflII C'TTAA_G
Cuts at: 0 1834 3750 4401
Size: 1834 1916 651
Fragments arranged by size:
1916 1834 651
AflIII A'CryG_T
Cuts at: 0 1043 1565 4256 4401
Size: 1043 522 2691 145
Fragments arranged by size:
2691 1043 522 145
AleI CACnn'nnGTG
Cuts at: 0 2322 4401
Size: 2322 2079
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 265 297 1565 1597 4401
Size: 265 32 1268 32 2804
Fragments arranged by size:
2804 1268 265 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1434 2991 3871 4087 4401
Size: 1434 1557 880 216 314
Fragments arranged by size:
1557 1434 880 314 216
ApaLI G'TGCA_C
Cuts at: 0 210 4401
Size: 210 4191
ApoI r'AATT_y
Cuts at: 0 2263 4401
Size: 2263 2138
AseI AT'TA_AT
Cuts at: 0 2393 3275 4401
Size: 2393 882 1126
Fragments arranged by size:
2393 1126 882
AvaI C'yCGr_G
Cuts at: 0 524 3563 3646 4401
Size: 524 3039 83 755
Fragments arranged by size:
3039 755 524 83
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3312 3345 3844 3877 4401
Size: 3312 33 499 33 524
Fragments arranged by size:
3312 524 499 33 33
BamHI G'GATC_C
Cuts at: 0 833 4401
Size: 833 3568
BanI G'GyrC_C
Cuts at: 0 257 694 1475 1543 1634 2299 2479 3734
Size: 257 437 781 68 91 665 180 1255
Cuts at: 3734 4041 4305 4401
Size: 307 264 96
Fragments arranged by size:
1255 781 665 437 307 264 257 180
96 91 68
BanII G_rGCy'C
Cuts at: 0 309 616 3583 4401
Size: 309 307 2967 818
Fragments arranged by size:
2967 818 309 307
BbeI G_GCGC'C
Cuts at: 0 261 4401
Size: 261 4140
BbsI GAAGACnn'nnnn_
Cuts at: 0 131 774 1651 2927 4401
Size: 131 643 877 1276 1474
Fragments arranged by size:
1474 1276 877 643 131
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1312 1346 4401
Size: 1312 34 3055
Fragments arranged by size:
3055 1312 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3872 4124 4401
Size: 3872 252 277
Fragments arranged by size:
3872 277 252
BglI GCCn_nnn'nGGC
Cuts at: 0 266 1072 1364 4401
Size: 266 806 292 3037
Fragments arranged by size:
3037 806 292 266
BglII A'GATC_T
Cuts at: 0 1859 2618 4401
Size: 1859 759 1783
Fragments arranged by size:
1859 1783 759
Bme1580I G_kGCm'C
Cuts at: 0 214 1965 4401
Size: 214 1751 2436
Fragments arranged by size:
2436 1751 214
BmgBI CAC'GTC
Cuts at: 0 48 1932 4401
Size: 48 1884 2469
Fragments arranged by size:
2469 1884 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 171 1282 1481 3733 4401
Size: 171 1111 199 2252 668
Fragments arranged by size:
2252 1111 668 199 171
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 439 487 638 749 889 1684 2162
Size: 76 363 48 151 111 140 795 478
Cuts at: 2162 3126 4401
Size: 964 1275
Fragments arranged by size:
1275 964 795 478 363 151 140 111
76 48
Bpu10I CC'TnA_GC
Cuts at: 0 253 4401
Size: 253 4148
BsaBI GATnn'nnATC
Cuts at: 0 2675 4401
Size: 2675 1726
BsaHI Gr'CG_yC
Cuts at: 0 258 728 959 1619 4401
Size: 258 470 231 660 2782
Fragments arranged by size:
2782 660 470 258 231
BsaWI w'CCGG_w
Cuts at: 0 836 1637 4401
Size: 836 801 2764
Fragments arranged by size:
2764 836 801
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 3113 3143 3185 3215 4151 4181
Size: 265 30 2818 30 42 30 936 30
Cuts at: 4181 4401
Size: 220
Fragments arranged by size:
2818 936 265 220 42 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 88 91 94 97 100 115 512
Size: 85 3 3 3 3 3 15 397
Cuts at: 512 637 784 1216 1695 4401
Size: 125 147 432 479 2706
Fragments arranged by size:
2706 479 432 397 147 125 85 15
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1818 2410 3937 4138 4216 4278 4401
Size: 1818 592 1527 201 78 62 123
Fragments arranged by size:
1818 1527 592 201 123 78 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1636 1696 2704 3097 3209 3223 3862
Size: 619 1017 60 1008 393 112 14 639
Cuts at: 3862 4401
Size: 539
Fragments arranged by size:
1017 1008 639 619 539 393 112 60
14
BsiHKAI G_wGCw'C
Cuts at: 0 214 309 616 1139 2319 3583 4401
Size: 214 95 307 523 1180 1264 818
Fragments arranged by size:
1264 1180 818 523 307 214 95
BsmI GAATG_Cn'
Cuts at: 0 1840 4401
Size: 1840 2561
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3507 3900 4401
Size: 3507 393 501
Fragments arranged by size:
3507 501 393
Bsp1286I G_dGCh'C
Cuts at: 0 214 309 616 1139 1965 2319 3583 4401
Size: 214 95 307 523 826 354 1264 818
Fragments arranged by size:
1264 826 818 523 354 307 214 95
BspHI T'CATG_A
Cuts at: 0 4323 4401
Size: 4323 78
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 137 3792 4401
Size: 137 3655 609
Fragments arranged by size:
3655 609 137
BsrBI CCG'CTC
Cuts at: 0 1448 2385 4401
Size: 1448 937 2016
Fragments arranged by size:
2016 1448 937
BsrDI GCAATG_nn'
Cuts at: 0 1600 2848 3684 4401
Size: 1600 1248 836 717
Fragments arranged by size:
1600 1248 836 717
BsrFI r'CCGG_y
Cuts at: 0 1005 3806 4401
Size: 1005 2801 595
Fragments arranged by size:
2801 1005 595
BsrGI T'GTAC_A
Cuts at: 0 1217 3261 4401
Size: 1217 2044 1140
Fragments arranged by size:
2044 1217 1140
BssSI C'ACGA_G
Cuts at: 0 1154 4401
Size: 1154 3247
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1156 3741 4401
Size: 1156 2585 660
Fragments arranged by size:
2585 1156 660
BstBI TT'CG_AA
Cuts at: 0 2331 4401
Size: 2331 2070
BstEII G'GTnAC_C
Cuts at: 0 3213 4401
Size: 3213 1188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2490 3724 3996 4401
Size: 2490 1234 272 405
Fragments arranged by size:
2490 1234 405 272
BstYI r'GATC_y
Cuts at: 0 833 1754 1859 2216 2618 3344 4401
Size: 833 921 105 357 402 726 1057
Fragments arranged by size:
1057 921 833 726 402 357 105
BstZ17I GTA'TAC
Cuts at: 0 1772 2550 4401
Size: 1772 778 1851
Fragments arranged by size:
1851 1772 778
Bsu36I CC'TnA_GG
Cuts at: 0 18 479 1613 3405 3482 4401
Size: 18 461 1134 1792 77 919
Fragments arranged by size:
1792 1134 919 461 77 18
BtgI C'CryG_G
Cuts at: 0 205 3303 4070 4401
Size: 205 3098 767 331
Fragments arranged by size:
3098 767 331 205
BtsI GCAGTG_nn'
Cuts at: 0 101 570 576 657 894 3642 4085 4401
Size: 101 469 6 81 237 2748 443 316
Fragments arranged by size:
2748 469 443 316 237 101 81 6
DraI TTT'AAA
Cuts at: 0 1113 4401
Size: 1113 3288
DraIII CAC_nnn'GTG
Cuts at: 0 642 1695 3741 4401
Size: 642 1053 2046 660
Fragments arranged by size:
2046 1053 660 642
DrdI GACnn_nn'nnGTC
Cuts at: 0 3680 4401
Size: 3680 721
EaeI y'GGCC_r
Cuts at: 0 1910 2559 3059 3491 3714 3773 4401
Size: 1910 649 500 432 223 59 628
Fragments arranged by size:
1910 649 628 500 432 223 59
EarI CTCTTCn'nnn_
Cuts at: 0 62 295 389 677 970 1022 4071 4401
Size: 62 233 94 288 293 52 3049 330
Fragments arranged by size:
3049 330 293 288 233 94 62 52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 958 1612 4401
Size: 958 654 2789
Fragments arranged by size:
2789 958 654
EcoICRI GAG'CTC
Cuts at: 0 307 614 3581 4401
Size: 307 307 2967 820
Fragments arranged by size:
2967 820 307 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 227 439 487 638 749 889 1003
Size: 76 151 212 48 151 111 140 114
Cuts at: 1003 1082 1109 1255 1456 1684 1720 1879 2162
Size: 79 27 146 201 228 36 159 283
Cuts at: 2162 2824 2994 3126 3404 3520 4401
Size: 662 170 132 278 116 881
Fragments arranged by size:
881 662 283 278 228 212 201 170
159 151 151 146 140 132 116 114
111 79 76 48 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 478 1897 2188 2376 3674 4401
Size: 478 1419 291 188 1298 727
Fragments arranged by size:
1419 1298 727 478 291 188
EcoO109I rG'GnC_Cy
Cuts at: 0 288 741 1923 2118 3418 4051 4401
Size: 288 453 1182 195 1300 633 350
Fragments arranged by size:
1300 1182 633 453 350 288 195
EcoRI G'AATT_C
Cuts at: 0 2263 4401
Size: 2263 2138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1923 4401
Size: 1923 2478
FspI TGC'GCA
Cuts at: 0 2963 4401
Size: 2963 1438
HaeII r_GCGC'y
Cuts at: 0 261 417 4401
Size: 261 156 3984
Fragments arranged by size:
3984 261 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 127 159 1137 1165 1169 1197 1385 1417
Size: 127 32 978 28 4 28 188 32
Cuts at: 1417 1851 1883 2830 2862 4401
Size: 434 32 947 32 1539
Fragments arranged by size:
1539 978 947 434 188 127 32 32
32 32 28 28 4
HincII GTy'rAC
Cuts at: 0 750 4401
Size: 750 3651
HindIII A'AGCT_T
Cuts at: 0 2203 4401
Size: 2203 2198
KasI G'GCGC_C
Cuts at: 0 257 4401
Size: 257 4144
KpnI G_GTAC'C
Cuts at: 0 1479 1638 2303 4401
Size: 1479 159 665 2098
Fragments arranged by size:
2098 1479 665 159
MfeI C'AATT_G
Cuts at: 0 361 4401
Size: 361 4040
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 3373 4401
Size: 380 2993 1028
Fragments arranged by size:
2993 1028 380
MscI TGG'CCA
Cuts at: 0 1912 2561 3061 3493 3716 3775 4401
Size: 1912 649 500 432 223 59 626
Fragments arranged by size:
1912 649 626 500 432 223 59
MslI CAynn'nnrTG
Cuts at: 0 704 1874 2106 2322 3094 4401
Size: 704 1170 232 216 772 1307
Fragments arranged by size:
1307 1170 772 704 232 216
MspA1I CmG'CkG
Cuts at: 0 207 465 1811 1967 3984 4282 4401
Size: 207 258 1346 156 2017 298 119
Fragments arranged by size:
2017 1346 298 258 207 156 119
NaeI GCC'GGC
Cuts at: 0 3808 4401
Size: 3808 593
NarI GG'CG_CC
Cuts at: 0 258 4401
Size: 258 4143
NdeI CA'TA_TG
Cuts at: 0 2103 4401
Size: 2103 2298
NgoMIV G'CCGG_C
Cuts at: 0 3806 4401
Size: 3806 595
NspI r_CATG'y
Cuts at: 0 159 175 1047 1569 2652 3112 4239 4260
Size: 159 16 872 522 1083 460 1127 21
Cuts at: 4260 4270 4401
Size: 10 131
Fragments arranged by size:
1127 1083 872 522 460 159 131 21
16 10
PasI CC'CwG_GG
Cuts at: 0 3414 4401
Size: 3414 987
PciI A'CATG_T
Cuts at: 0 1043 1565 4256 4401
Size: 1043 522 2691 145
Fragments arranged by size:
2691 1043 522 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 918 3627 3871 4285 4401
Size: 918 2709 244 414 116
Fragments arranged by size:
2709 918 414 244 116
PfoI T'CCnGG_A
Cuts at: 0 350 1793 2075 2509 3237 3814 4401
Size: 350 1443 282 434 728 577 587
Fragments arranged by size:
1443 728 587 577 434 350 282
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 265 297 3654 3686 4327 4359 4401
Size: 265 32 3357 32 641 32 42
Fragments arranged by size:
3357 641 265 42 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 288 741 1923 2118 3418 4401
Size: 288 453 1182 195 1300 983
Fragments arranged by size:
1300 1182 983 453 288 195
PshAI GACnn'nnGTC
Cuts at: 0 2143 4401
Size: 2143 2258
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1762 1794 4401
Size: 1762 32 2607
Fragments arranged by size:
2607 1762 32
PstI C_TGCA'G
Cuts at: 0 1953 4401
Size: 1953 2448
PvuII CAG'CTG
Cuts at: 0 3984 4282 4401
Size: 3984 298 119
Fragments arranged by size:
3984 298 119
SacI G_AGCT'C
Cuts at: 0 309 616 3583 4401
Size: 309 307 2967 818
Fragments arranged by size:
2967 818 309 307
SacII CC_GC'GG
Cuts at: 0 208 4401
Size: 208 4193
ScaI AGT'ACT
Cuts at: 0 1504 4401
Size: 1504 2897
SexAI A'CCwGG_T
Cuts at: 0 2295 4401
Size: 2295 2106
SfcI C'TryA_G
Cuts at: 0 1949 2050 4401
Size: 1949 101 2351
Fragments arranged by size:
2351 1949 101
SfoI GGC'GCC
Cuts at: 0 259 4401
Size: 259 4142
SgrAI Cr'CCGG_yG
Cuts at: 0 3806 4401
Size: 3806 595
SmaI CCC'GGG
Cuts at: 0 3565 3648 4401
Size: 3565 83 753
Fragments arranged by size:
3565 753 83
SmlI C'TyrA_G
Cuts at: 0 1834 3750 4401
Size: 1834 1916 651
Fragments arranged by size:
1916 1834 651
SphI G_CATG'C
Cuts at: 0 175 4401
Size: 175 4226
StyI C'CwwG_G
Cuts at: 0 264 732 1539 1718 2009 2114 2246 2268
Size: 264 468 807 179 291 105 132 22
Cuts at: 2268 2825 2882 4401
Size: 557 57 1519
Fragments arranged by size:
1519 807 557 468 291 264 179 132
105 57 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 712 1167 1275 1301 2452 3480 3752 4401
Size: 712 455 108 26 1151 1028 272 649
Fragments arranged by size:
1151 1028 712 649 455 272 108 26
TatI w'GTAC_w
Cuts at: 0 1217 1502 3261 4401
Size: 1217 285 1759 1140
Fragments arranged by size:
1759 1217 1140 285
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 142 174 3185 3217 4151 4183 4401
Size: 142 32 3011 32 934 32 218
Fragments arranged by size:
3011 934 218 142 32 32 32
XbaI T'CTAG_A
Cuts at: 0 1302 2607 4401
Size: 1302 1305 1794
Fragments arranged by size:
1794 1305 1302
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2490 3301 4401
Size: 2490 811 1100
Fragments arranged by size:
2490 1100 811
XmaI C'CCGG_G
Cuts at: 0 3563 3646 4401
Size: 3563 83 755
Fragments arranged by size:
3563 755 83
XmnI GAAnn'nnTTC
Cuts at: 0 1892 4401
Size: 1892 2509
ZraI GAC'GTC
Cuts at: 0 960 1620 4401
Size: 960 660 2781
Fragments arranged by size:
2781 960 660
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflII AflIII AleI
AloI AlwNI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbeI BbsI BcgI BciVI BglI BglII
Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtsI
DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PshAI PsrI PstI PvuII SacI
SacII ScaI SexAI SfcI SfoI SgrAI SmaI SmlI
SphI StyI TaqII TatI TstI XbaI XcmI XmaI
XmnI ZraI
Enzymes that do not cut:
AclI AfeI AgeI AhdI AlfI ApaI AscI AsiSI
AvrII BbvCI BclI BfrBI BlpI BmtI BplI BpuEI
BsaI BsaAI BsiEI BsiWI BspEI BssHII BtgZI ClaI
CspCI EagI EcoRV FseI FspAI HpaI MluI NcoI
NheI NotI NruI NsiI PacI PmeI PmlI PsiI
PspOMI PspXI PvuI RsrII SalI SanDI SapI SbfI
SfiI SgfI SnaBI SpeI SrfI SspI StuI SwaI
Tth111I XhoI