################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## Acc65I AccI AccII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AvaI AviII AvrII BalI BbeI BbrPI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MseI MslI MspA1I MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StyI SwaI TaqI Tru9I TthHB8I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 745 GACGTC GACGT^C AccIII 443 TCCGGA T^CCGGA AciI 198 GCGG CCGC(-3/-1) AflIII 757 ACATGT A^C[GA][CT]GT AlwNI 534 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 619 GGGCCC GGGCC^C AspHI 137 GTGCTC G[AT]GC[AT]^C AspI 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 672 GGATCC G^GATCC BanI 200 GGCACC G^G[CT][GA]CC BanII 619 GGGCCC G[GA]GC[CT]^C BbsI 469 GTCTTC GAAGAC(2/6) BbvCI 183 GCTGAGG CCTCAGC(-5/-2) BcgI 830 CGAAGCCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 446 GGATAC GTATCC(6/5) BpuAI 469 GTCTTC GAAGAC(2/6) BsaWI 443 TCCGGA [AT]^CCGG[AT] BseAI 443 TCCGGA T^CCGGA BsgI 121 GTGCAG GTGCAG(16/14) BsiHKAI 137 GTGCTC G[AT]GC[AT]^C BsmFI 463 GTCCC GGGAC(10/14) BsmI 552 GAATGC GAATGC(1/-1) BspEI 443 TCCGGA T^CCGGA BspHI 810 TCATGA T^CATGA BspLU11I 757 ACATGT A^CATGT BstF5I 516 CATCC GGATG(2/0) FokI 516 CATCC GGATG(9/13) HgaI 168 GCGTC GACGC(5/10) MroI 443 TCCGGA T^CCGGA PflFI 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 719 AGGACCT [GA]G^G[AT]CC[CT] RcaI 810 TCATGA T^CATGA SapI 106 GAAGAGC GCTCTTC(1/4) ScaI 379 AGTACT AGT^ACT SgrAI 202 CACCGGCG C[GA]^CCGG[CT]G SspI 500 AATATT AAT^ATT StuI 333 AGGCCT AGG^CCT Tsp509I 290 AATT ^AATT Tth111I 421 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 245 CCAACCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 167 GGCGTC G[GA]^CG[CT]C 745 GACGTC AvaII 420 GGACC G^G[AT]CC 720 GGACC BfaI 70 CTAG C^TAG 704 CTAG BmyI 137 GTGCTC G[AGT]GC[ACT]^C 619 GGGCCC BsaHI 167 GGCGTC G[GA]^CG[CT]C 745 GACGTC BsaI 73 GAGACC GGTCTC(1/5) 894 GGTCTC BseRI 359 GAGGAG GAGGAG(10/8) 793 GAGGAG Bsp1286I 137 GTGCTC G[AGT]GC[ACT]^C 619 GGGCCC BsrFI 203 ACCGGC [GA]^CCGG[CT] 584 ACCGGC BstAPI 114 GCAGAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 223 GCATGTATTGC BstYI 88 AGATCC [GA]^GATC[CT] 672 GGATCC Cfr10I 203 ACCGGC [GA]^CCGG[CT] 584 ACCGGC DraII 619 GGGCCCT [GA]G^G[ACGT]CC[CT] 719 AGGACCT EcoO109I 619 GGGCCCT [GA]G^G[ACGT]CC[CT] 719 AGGACCT Hin1I 167 GGCGTC G[GA]^CG[CT]C 745 GACGTC HphI 453 TCACC GGTGA(8/7) 749 TCACC MaeI 70 CTAG C^TAG 704 CTAG MaeII 21 ACGT A^CGT 746 ACGT MflI 88 AGATCC [GA]^GATC[CT] 672 GGATCC NspI 223 GCATGT [GA]CATG^[CT] 757 ACATGT PflMI 249 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 782 CCACTTCATGG PleI 34 GAGTC GAGTC(4/5) 888 GAGTC PstI 112 CTGCAG CTGCA^G 313 CTGCAG SfcI 112 CTGCAG C^T[GA][CT]AG 313 CTGCAG TfiI 153 GATTC G^A[AT]TC 406 GATTC Van91I 249 CCAAACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 782 CCACTTCATGG XhoII 88 AGATCC [GA]^GATC[CT] 672 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AfaI AluI AlwI BbvI BsaJI BsiYI BslI BsmAI BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI Tsp45I TspRI