(Circular) (Six-base) MAPSORT of: pFN21AE9611.seq Check: 6528 from: 1 to: 5605 pFN21AE9611 5605 bp With 182 enzymes: SgfI * March 28, 2012 12:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2754 3415 278 Size: 53 83 186 936 1218 661 2468 Fragments arranged by size: 2468 1218 936 661 186 83 53 AccI GT'mk_AC Cuts at: 1937 2948 1937 Size: 1011 4594 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2130 2579 3668 5052 5320 1632 Size: 498 449 1089 1384 268 1917 Fragments arranged by size: 1917 1384 1089 498 449 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 2762 4504 5481 1203 Size: 1559 1742 977 1327 Fragments arranged by size: 1742 1559 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5605 AlwNI CAG_nnn'CTG Cuts at: 2544 3348 4920 2544 Size: 804 1572 3229 Fragments arranged by size: 3229 1572 804 ApaI G_GGCC'C Cuts at: 2628 2628 Size: 5605 ApaLI G'TGCA_C Cuts at: 3662 4818 3662 Size: 1156 4449 ApoI r'AATT_y Cuts at: 1493 2914 3140 3292 5401 1493 Size: 1421 226 152 2109 1697 Fragments arranged by size: 2109 1697 1421 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2930 1870 Size: 73 987 4545 Fragments arranged by size: 4545 987 73 BamHI G'GATC_C Cuts at: 2677 2935 3302 2677 Size: 258 367 4980 Fragments arranged by size: 4980 367 258 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2205 2926 4381 618 Size: 325 217 627 418 721 1455 1842 Fragments arranged by size: 1842 1455 721 627 418 325 217 BanII G_rGCy'C Cuts at: 1391 2628 2924 1391 Size: 1237 296 4072 Fragments arranged by size: 4072 1237 296 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2467 928 Size: 971 568 4066 Fragments arranged by size: 4066 971 568 BbvCI CC'TCA_GC Cuts at: 2189 2189 Size: 5605 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2824 2858 3796 3830 1903 Size: 34 887 34 938 34 3678 Fragments arranged by size: 3678 938 887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2445 3497 4707 2445 Size: 1052 1210 3343 Fragments arranged by size: 3343 1210 1052 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4216 136 Size: 107 122 71 631 605 2544 1525 Fragments arranged by size: 2544 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2628 3666 4822 1790 Size: 838 1038 1156 2573 Fragments arranged by size: 2573 1156 1038 838 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5605 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2465 2631 3336 Size: 416 53 83 399 24 1048 166 705 Cuts at: 3336 4294 442 Size: 958 1753 Fragments arranged by size: 1753 1048 958 705 416 399 166 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4265 1239 Size: 3026 2579 Bpu10I CC'TnA_GC Cuts at: 2189 2189 Size: 5605 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2072 2905 4268 882 Size: 617 573 833 1363 2219 Fragments arranged by size: 2219 1363 833 617 573 BsaAI yAC'GTr Cuts at: 493 1204 3396 493 Size: 711 2192 2702 Fragments arranged by size: 2702 2192 711 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2173 2751 3412 3794 Size: 53 83 186 936 640 578 661 382 Cuts at: 3794 4429 275 Size: 635 1451 Fragments arranged by size: 1451 936 661 640 635 578 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2448 4038 4436 4710 4857 5268 2448 Size: 1590 398 274 147 411 2785 Fragments arranged by size: 2785 1590 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2358 2388 2763 2793 1178 Size: 30 165 30 955 30 375 30 3990 Fragments arranged by size: 3990 955 375 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2379 2813 1186 Size: 1193 434 3978 Fragments arranged by size: 3978 1193 434 BseYI C'CCAG_C Cuts at: 4808 5304 4808 Size: 496 5109 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2147 1163 Size: 573 411 4621 Fragments arranged by size: 4621 573 411 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2146 3666 3751 4822 2146 Size: 1520 85 1071 2929 Fragments arranged by size: 2929 1520 1071 85 BsmI GAATG_Cn' Cuts at: 2563 3116 3209 2563 Size: 553 93 4959 Fragments arranged by size: 4959 553 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2628 2924 3666 3751 4822 1391 Size: 399 356 482 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 356 296 85 BspEI T'CCGG_A Cuts at: 2448 5268 2448 Size: 2820 2785 BspHI T'CATG_A Cuts at: 2815 3494 5224 2815 Size: 679 1730 3196 Fragments arranged by size: 3196 1730 679 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3492 1394 Size: 429 1669 3507 Fragments arranged by size: 3507 1669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2208 2589 4249 4436 1700 Size: 110 398 381 1660 187 2869 Fragments arranged by size: 2869 1660 398 381 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 2125 2234 794 Size: 1331 109 4165 Fragments arranged by size: 4165 1331 109 BstBI TT'CG_AA Cuts at: 4409 4409 Size: 5605 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2093 2677 2935 3302 3687 3704 5156 5242 Size: 121 584 258 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 584 385 367 346 258 121 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5570 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3052 3941 5570 Size: 54 1425 50 275 18 1222 889 1629 Cuts at: 5570 8 Size: 43 Fragments arranged by size: 1629 1425 1222 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2106 2283 2484 2745 3535 1353 Size: 753 177 201 261 790 3423 Fragments arranged by size: 3423 790 753 261 201 177 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2130 2579 3668 4265 5052 5320 1239 Size: 393 498 449 1089 597 787 268 1524 Fragments arranged by size: 1524 1089 787 597 498 449 393 268 EcoO109I rG'GnC_Cy Cuts at: 2625 2725 2625 Size: 100 5505 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2162 2162 Size: 5605 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 4752 1678 Size: 3074 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2358 2390 4246 Size: 32 135 32 452 32 302 32 1856 Cuts at: 4246 4278 4320 4352 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1856 452 302 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2949 3195 669 Size: 988 281 1011 246 3079 Fragments arranged by size: 3079 1011 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5605 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2865 4719 4903 1305 Size: 1560 1854 184 2007 Fragments arranged by size: 2007 1854 1560 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3783 Fragments arranged by size: 3783 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3563 3922 4081 518 Size: 648 2397 359 159 2042 Fragments arranged by size: 2397 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3021 3698 4846 5091 1606 Size: 1415 677 1148 245 2120 Fragments arranged by size: 2120 1415 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4114 Fragments arranged by size: 4114 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2232 2766 2963 4508 1132 Size: 97 1003 534 197 1545 2229 Fragments arranged by size: 2229 1545 1003 534 197 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 2762 4504 2762 Size: 1742 3863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2260 2793 1108 Size: 1152 533 3920 Fragments arranged by size: 3920 1152 533 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2725 Size: 5605 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PspOMI G'GGCC_C Cuts at: 2624 2624 Size: 5605 PstI C_TGCA'G Cuts at: 830 2121 2322 2957 830 Size: 1291 201 635 3478 Fragments arranged by size: 3478 1291 635 201 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SapI GCTCTTCn'nnn_ Cuts at: 2106 2106 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 2386 3853 5565 2386 Size: 1467 1712 2426 Fragments arranged by size: 2426 1712 1467 SfcI C'TryA_G Cuts at: 826 1045 2117 2318 2953 4088 4769 4960 826 Size: 219 1072 201 635 1135 681 191 1471 Fragments arranged by size: 1471 1135 1072 681 635 219 201 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 2208 1700 Size: 508 5097 SmaI CCC'GGG Cuts at: 1872 2932 1872 Size: 1060 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3396 493 Size: 2903 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 2507 3529 5 Size: 47 2455 1022 2081 Fragments arranged by size: 2455 2081 1022 47 StuI AGG'CCT Cuts at: 1517 2340 1517 Size: 823 4782 StyI C'CwwG_G Cuts at: 513 1065 2004 3044 513 Size: 552 939 1040 3074 Fragments arranged by size: 3074 1040 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3632 3649 3802 3987 4432 325 Size: 1255 2052 17 153 185 445 1498 Fragments arranged by size: 2052 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2384 3851 5563 Size: 274 80 33 51 1443 407 1467 1712 Cuts at: 5563 96 Size: 138 Fragments arranged by size: 1712 1467 1443 407 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5565 5597 5565 Size: 32 5573 Tth111I GACn'n_nGTC Cuts at: 1316 2429 1316 Size: 1113 4492 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2257 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2930 1870 Size: 1060 4545 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2752 3413 276 Size: 53 83 186 936 1218 661 2468 Fragments arranged by size: 2468 1218 936 661 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI