################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII BalI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlpI Bpu1102I BsaAI BsaBI BsaHI BsiWI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MscI MseI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccIII 137 TCCGGA T^CCGGA AlwNI 304 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AspI 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 871 CCCGAG C^[CT]CG[GA]G AvrII 540 CCTAGG C^CTAGG BamHI 867 GGATCC G^GATCC BanI 609 GGCACC G^G[CT][GA]CC BbsI 511 GTCTTC GAAGAC(2/6) BlnI 540 CCTAGG C^CTAGG BpuAI 511 GTCTTC GAAGAC(2/6) BsaWI 137 TCCGGA [AT]^CCGG[AT] BseAI 137 TCCGGA T^CCGGA BsgI 284 CTGCAC GTGCAG(16/14) BsiEI 162 CGGCCG CG[GA][CT]^CG BsmBI 279 CGTCTC CGTCTC(1/5) BsoBI 871 CCCGAG C^[CT]CG[GA]G BspEI 137 TCCGGA T^CCGGA BstXI 805 CCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 867 GGATCC [GA]^GATC[CT] DpnII 868 GATC ^GATC EagI 162 CGGCCG C^GGCCG EarI 694 CTCTTC CTCTTC(1/4) EclXI 162 CGGCCG C^GGCCG Eco52I 162 CGGCCG C^GGCCG EcoNI 248 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 964 AGCGCC [GA]GCGC^[CT] HinfI 670 GACTC G^A[ACGT]TC Ksp632I 694 CTCTTC CTCTTC(1/4) MboI 868 GATC ^GATC MflI 867 GGATCC [GA]^GATC[CT] MroI 137 TCCGGA T^CCGGA MslI 481 CACGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeII 868 GATC ^GATC PflFI 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 66 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 670 GACTC GAGTC(4/5) PshAI 547 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 200 CAGCTG CAG^CTG Sau3AI 868 GATC ^GATC SfaNI 384 GATGC GCATC(5/9) SmlI 999 CTCAAG C^T[CT][GA]AG Tth111I 517 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 66 CCAACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 802 CCACCACCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 867 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccII 166 CGCG CG^CG 360 CGCG AfaI 126 GTAC GT^AC 357 GTAC AlwI 868 GATCC GGATC(4/5) 867 GGATC AocI 25 CCTGAGG CC^T[ACGT]AGG 252 CCTGAGG BglI 433 GCCCAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 651 GCCTCTGCGGC BpmI 847 CTCCAG CTGGAG(16/14) 1006 CTGGAG BsaI 19 GAGACC GGTCTC(1/5) 545 GAGACC BstEII 96 GGTGACC G^GT[ACGT]ACC 774 GGTGACC BstPI 96 GGTGACC G^GT[ACGT]ACC 774 GGTGACC BstUI 166 CGCG CG^CG 360 CGCG Bsu36I 25 CCTGAGG CC^T[ACGT]AGG 252 CCTGAGG DsaI 605 CCACGG C^C[GA][CT]GG 665 CCGTGG Eco81I 25 CCTGAGG CC^T[ACGT]AGG 252 CCTGAGG EcoO65I 96 GGTGACC G^GT[ACGT]ACC 774 GGTGACC EcoT14I 540 CCTAGG C^C[AT][AT]GG 721 CCTTGG HgaI 222 GCGTC GACGC(5/10) 278 GCGTC MaeII 503 ACGT A^CGT 728 ACGT MaeIII 97 GTGAC ^GT[ACGT]AC 775 GTGAC MboII 512 TCTTC GAAGA(8/7) 695 TCTTC MvnI 166 CGCG CG^CG 360 CGCG RsaI 126 GTAC GT^AC 357 GTAC StuI 294 AGGCCT AGG^CCT 758 AGGCCT StyI 540 CCTAGG C^C[AT][AT]GG 721 CCTTGG TaqI 515 TCGA T^CGA 755 TCGA Tsp45I 97 GTGAC ^GT[GC]AC 775 GTGAC TthHB8I 515 TCGA T^CGA 755 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AluI AspHI AvaII BanII BbvCI BbvI BcnI BfaI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeI MnlI MspA1I MspI MvaI MwoI NciI NlaIV PpuMI PstI SacI Sau96I ScrFI SfcI TseI TspRI