(Circular) (Six-base) MAPSORT of: pFN21AE3808.seq Check: 34 from: 1 to: 5782 pFN21AE3808 5782 bp With 182 enzymes: SgfI * March 28, 2012 12:13 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3592 278 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3125 1937 Size: 1188 4594 Acc65I G'GTAC_C Cuts at: 3103 3103 Size: 5782 AclI AA'CG_TT Cuts at: 1563 3909 4282 1563 Size: 2346 373 3063 Fragments arranged by size: 3063 2346 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 3845 5229 5497 1632 Size: 605 1608 1384 268 1917 Fragments arranged by size: 1917 1608 1384 605 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5585 AflIII A'CryG_T Cuts at: 1203 4681 5658 1203 Size: 3478 977 1327 Fragments arranged by size: 3478 1327 977 AgeI A'CCGG_T Cuts at: 4613 4613 Size: 5782 AhdI GACnn_n'nnGTC Cuts at: 4511 4511 Size: 5782 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5782 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5782 AlwNI CAG_nnn'CTG Cuts at: 2314 3525 5097 2314 Size: 1211 1572 2999 Fragments arranged by size: 2999 1572 1211 ApaLI G'TGCA_C Cuts at: 3839 4995 3839 Size: 1156 4626 ApoI r'AATT_y Cuts at: 1493 3091 3317 3469 5578 1493 Size: 1598 226 152 2109 1697 Fragments arranged by size: 2109 1697 1598 226 152 AseI AT'TA_AT Cuts at: 160 1032 4336 160 Size: 872 3304 1606 Fragments arranged by size: 3304 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 AvaI C'yCGr_G Cuts at: 1870 1943 2876 3107 1870 Size: 73 933 231 4545 Fragments arranged by size: 4545 933 231 73 AvrII C'CTAG_G Cuts at: 2545 2545 Size: 5782 BamHI G'GATC_C Cuts at: 2872 3112 3479 2872 Size: 240 367 5175 Fragments arranged by size: 5175 367 240 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2614 3103 4558 618 Size: 325 217 627 827 489 1455 1842 Fragments arranged by size: 1842 1455 827 627 489 325 217 BanII G_rGCy'C Cuts at: 1391 2156 2455 2628 2891 3079 3101 1391 Size: 765 299 173 263 188 22 4072 Fragments arranged by size: 4072 765 299 263 188 173 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2509 928 Size: 971 610 4201 Fragments arranged by size: 4201 971 610 BbvCI CC'TCA_GC Cuts at: 2381 2793 2802 2381 Size: 412 9 5361 Fragments arranged by size: 5361 412 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3973 4007 1903 Size: 34 2036 34 3678 Fragments arranged by size: 3678 2036 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3674 4884 3674 Size: 1210 4572 BclI T'GATC_A Cuts at: 1548 1548 Size: 5782 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2444 2662 4393 Size: 107 122 71 631 605 772 218 1731 Cuts at: 4393 136 Size: 1525 Fragments arranged by size: 1731 1525 772 631 605 218 122 107 71 BglII A'GATC_T Cuts at: 5777 5777 Size: 5782 BlpI GC'TnA_GC Cuts at: 3199 3199 Size: 5782 Bme1580I G_kGCm'C Cuts at: 1790 3843 4999 1790 Size: 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 BmgBI CAC'GTC Cuts at: 1318 2734 1318 Size: 1416 4366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2434 2519 2935 Size: 416 53 83 399 24 1017 85 416 Cuts at: 2935 3513 4471 442 Size: 578 958 1753 Fragments arranged by size: 1753 1017 958 578 416 416 399 85 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5782 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3019 3019 Size: 5782 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2837 3032 4442 1239 Size: 1598 195 1410 2579 Fragments arranged by size: 2579 1598 1410 195 Bpu10I CC'TnA_GC Cuts at: 2381 2622 2793 2802 2381 Size: 241 171 9 5361 Fragments arranged by size: 5361 241 171 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2989 3907 4772 5070 2989 Size: 918 865 298 3701 Fragments arranged by size: 3701 918 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2018 2544 4445 882 Size: 617 519 526 1901 2219 Fragments arranged by size: 2219 1901 617 526 519 BsaAI yAC'GTr Cuts at: 493 1204 3573 493 Size: 711 2369 2702 Fragments arranged by size: 2702 2369 711 BsaBI GATnn'nnATC Cuts at: 5776 5776 Size: 5782 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3589 3971 4606 275 Size: 53 83 186 936 2056 382 635 1451 Fragments arranged by size: 2056 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 4215 4613 4887 5034 5445 2142 Size: 2073 398 274 147 411 2479 Fragments arranged by size: 2479 2073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3066 3096 1178 Size: 30 165 30 1663 30 3864 Fragments arranged by size: 3864 1663 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2063 2128 2168 3008 3029 1186 Size: 877 65 40 840 21 3939 Fragments arranged by size: 3939 877 840 65 40 21 BseYI C'CCAG_C Cuts at: 2149 2184 2775 2836 4985 5481 2149 Size: 35 591 61 2149 496 2450 Fragments arranged by size: 2450 2149 591 496 61 35 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2274 1163 Size: 573 538 4671 Fragments arranged by size: 4671 573 538 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 3232 3993 5021 1540 Size: 275 186 169 1062 761 1028 2301 Fragments arranged by size: 2301 1062 1028 761 275 186 169 BsiHKAI G_wGCw'C Cuts at: 2178 2455 2628 3079 3843 3928 4999 2178 Size: 277 173 451 764 85 1071 2961 Fragments arranged by size: 2961 1071 764 451 277 173 85 BsmI GAATG_Cn' Cuts at: 3293 3386 3293 Size: 93 5689 BsmBI CGTCTCn'nnnn_ Cuts at: 2290 2290 Size: 5782 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2178 2455 2628 2891 3079 3101 Size: 399 366 22 277 173 263 188 22 Cuts at: 3101 3843 3928 4999 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 277 263 188 173 85 22 22 BspEI T'CCGG_A Cuts at: 2142 5445 2142 Size: 3303 2479 BspHI T'CATG_A Cuts at: 3671 5401 3671 Size: 1730 4052 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3137 844 Size: 2293 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3669 1394 Size: 429 1846 3507 Fragments arranged by size: 3507 1846 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4277 4451 65 Size: 1183 3029 174 1396 Fragments arranged by size: 3029 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4426 4613 1700 Size: 110 2616 187 2869 Fragments arranged by size: 2869 2616 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5782 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5782 BssSI C'ACGA_G Cuts at: 3842 4854 3842 Size: 1012 4770 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5782 BstBI TT'CG_AA Cuts at: 4586 4586 Size: 5782 BstEII G'GTnAC_C Cuts at: 1184 2101 2779 1184 Size: 917 678 4187 Fragments arranged by size: 4187 917 678 BstXI CCAn_nnnn'nTGG Cuts at: 2817 2817 Size: 5782 BstYI r'GATC_y Cuts at: 1972 2872 3112 3479 3864 3881 5333 5419 5431 Size: 900 240 367 385 17 1452 86 12 Cuts at: 5431 5777 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 900 385 367 346 240 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2031 2258 2031 Size: 227 5555 BtgI C'CryG_G Cuts at: 513 1065 2004 2610 2670 513 Size: 552 939 606 60 3625 Fragments arranged by size: 3625 939 606 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5043 Fragments arranged by size: 5043 683 56 BtsI GCAGTG_nn' Cuts at: 2712 3298 4092 4112 2712 Size: 586 794 20 4382 Fragments arranged by size: 4382 794 586 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5782 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3031 3066 529 Size: 35 2467 35 3245 Fragments arranged by size: 3245 2467 35 35 DraI TTT'AAA Cuts at: 3086 3086 Size: 5782 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4789 809 Size: 507 3473 1802 Fragments arranged by size: 3473 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2167 2567 2663 Size: 54 1425 50 275 18 337 400 96 Cuts at: 2663 3229 4118 5747 8 Size: 566 889 1629 43 Fragments arranged by size: 1629 1425 889 566 400 337 275 96 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2167 3229 1537 Size: 275 355 1062 4090 Fragments arranged by size: 4090 1062 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2705 3712 1353 Size: 1352 1007 3423 Fragments arranged by size: 3423 1352 1007 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4369 4743 4889 387 Size: 1064 138 2780 374 146 1280 Fragments arranged by size: 2780 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2453 2626 3077 2453 Size: 173 451 5158 Fragments arranged by size: 5158 451 173 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2237 2837 3032 3845 4442 5229 5497 Size: 393 605 600 195 813 597 787 268 Cuts at: 5497 1239 Size: 1524 Fragments arranged by size: 1524 813 787 605 600 597 393 268 195 EcoNI CCTnn'n_nnAGG Cuts at: 2257 2257 Size: 5782 EcoO109I rG'GnC_Cy Cuts at: 2016 2041 2321 2377 2404 2683 2951 2016 Size: 25 280 56 27 279 268 4847 Fragments arranged by size: 4847 280 279 268 56 27 25 EcoRI G'AATT_C Cuts at: 3091 3469 3091 Size: 378 5404 FspI TGC'GCA Cuts at: 4288 4288 Size: 5782 HaeII r_GCGC'y Cuts at: 1678 2973 4929 1678 Size: 1295 1956 2531 Fragments arranged by size: 2531 1956 1295 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4423 4455 4497 4529 1373 Size: 32 135 32 2851 32 42 32 2626 Fragments arranged by size: 2851 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3126 3372 669 Size: 988 281 1188 246 3079 Fragments arranged by size: 3079 1188 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5782 HpaI GTT'AAC Cuts at: 3372 3372 Size: 5782 KpnI G_GTAC'C Cuts at: 3107 3107 Size: 5782 MfeI C'AATT_G Cuts at: 3381 3381 Size: 5782 MluI A'CGCG_T Cuts at: 5658 5658 Size: 5782 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2095 4896 5080 1305 Size: 790 2801 184 2007 Fragments arranged by size: 2801 2007 790 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3960 Fragments arranged by size: 3960 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2490 3740 4099 4258 518 Size: 648 1324 1250 359 159 2042 Fragments arranged by size: 2042 1324 1250 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2207 2280 2385 3198 3875 5023 5268 1606 Size: 601 73 105 813 677 1148 245 2120 Fragments arranged by size: 2120 1148 813 677 601 245 105 73 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4291 Fragments arranged by size: 4291 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5782 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5782 NotI GC'GGCC_GC Cuts at: 3229 3229 Size: 5782 NspI r_CATG'y Cuts at: 1132 1229 3140 4685 1132 Size: 97 1911 1545 2229 Fragments arranged by size: 2229 1911 1545 97 PasI CC'CwG_GG Cuts at: 1348 2817 1348 Size: 1469 4313 PciI A'CATG_T Cuts at: 4681 4681 Size: 5782 PflMI CCAn_nnn'nTGG Cuts at: 1108 2077 1108 Size: 969 4813 PfoI T'CCnGG_A Cuts at: 2904 2904 Size: 5782 PmeI GTTT'AAAC Cuts at: 3086 3086 Size: 5782 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3851 3883 5364 5396 3851 Size: 32 1481 32 4237 Fragments arranged by size: 4237 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2041 2321 2377 2951 2041 Size: 280 56 574 4872 Fragments arranged by size: 4872 574 280 56 PshAI GACnn'nnGTC Cuts at: 1613 2556 1613 Size: 943 4839 PsiI TTA'TAA Cuts at: 3352 3352 Size: 5782 PstI C_TGCA'G Cuts at: 830 2310 2397 2534 3134 830 Size: 1480 87 137 600 3478 Fragments arranged by size: 3478 1480 600 137 87 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5782 PvuII CAG'CTG Cuts at: 2207 2207 Size: 5782 SacI G_AGCT'C Cuts at: 2455 2628 3079 2455 Size: 173 451 5158 Fragments arranged by size: 5158 451 173 SalI G'TCGA_C Cuts at: 1936 3124 1936 Size: 1188 4594 SanDI GG'GwC_CC Cuts at: 2041 2041 Size: 5782 SbfI CC_TGCA'GG Cuts at: 3134 3134 Size: 5782 ScaI AGT'ACT Cuts at: 4030 5742 4030 Size: 1712 4070 SfcI C'TryA_G Cuts at: 826 1045 2306 2327 2393 2530 3130 4265 4946 Size: 219 1261 21 66 137 600 1135 681 Cuts at: 4946 5137 826 Size: 191 1471 Fragments arranged by size: 1471 1261 1135 681 600 219 191 137 66 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5782 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5782 SmaI CCC'GGG Cuts at: 1872 3109 1872 Size: 1237 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3004 3886 4787 5049 820 Size: 197 926 1061 882 901 262 1553 Fragments arranged by size: 1553 1061 926 901 882 262 197 SnaBI TAC'GTA Cuts at: 493 3573 493 Size: 3080 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5782 SphI G_CATG'C Cuts at: 1132 3140 1132 Size: 2008 3774 SspI AAT'ATT Cuts at: 5 52 3706 5 Size: 47 3654 2081 Fragments arranged by size: 3654 2081 47 StuI AGG'CCT Cuts at: 1517 2301 2765 1517 Size: 784 464 4534 Fragments arranged by size: 4534 784 464 StyI C'CwwG_G Cuts at: 513 1065 2004 2545 2726 3221 513 Size: 552 939 541 181 495 3074 Fragments arranged by size: 3074 939 552 541 495 181 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2310 2767 3809 3826 3979 4164 4609 Size: 1255 730 457 1042 17 153 185 445 Cuts at: 4609 325 Size: 1498 Fragments arranged by size: 1498 1255 1042 730 457 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4028 5740 96 Size: 274 80 33 51 1443 2051 1712 138 Fragments arranged by size: 2051 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2886 2918 5742 5774 2886 Size: 32 2824 32 2894 Fragments arranged by size: 2894 2824 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2525 1316 Size: 1209 4573 XbaI T'CTAG_A Cuts at: 3118 3118 Size: 5782 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2814 2814 Size: 5782 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5782 XmaI C'CCGG_G Cuts at: 1870 3107 1870 Size: 1237 4545 XmnI GAAnn'nnTTC Cuts at: 3911 3911 Size: 5782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3590 276 Size: 53 83 186 936 2056 2468 Fragments arranged by size: 2468 2056 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI