################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccI AccII AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp700I AspEI AviII AvrII BfrI BlnI BsaBI BsiEI BsiWI BspLU11I BsrBI BsrFI BssHII Bst1107I BstEII BstPI BstUI BstZ17I Cfr10I CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HpaI KspI MamI MfeI MluI MunI MvnI NaeI NcoI NdeI NgoMIV NotI NruI PacI PflMI PinAI PmeI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I StuI Van91I XcmI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 1501 GGTACC G^GTACC AclI 1666 AACGTT AA^CGTT AflIII 3431 ACACGT A^C[GA][CT]GT AseI 921 ATTAAT AT^TAAT AsnI 921 ATTAAT AT^TAAT Asp718I 1501 GGTACC G^GTACC AspI 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 1368 GGCGCC GGCGC^C BbrPI 4219 CACGTG CAC^GTG BciVI 1445 GTATCC GTATCC(6/5) BclI 2381 TGATCA T^GATCA BglI 3826 GCCGGGACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 3478 AGATCT A^GATCT BlpI 1825 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1825 GCTCAGC GC^T[ACGT]AGC BsaAI 4219 CACGTG [CT]AC^GT[GA] BspDI 3076 ATCGAT AT^CGAT BspHI 768 TCATGA T^CATGA BssSI 2800 CACGAG CACGAG(-5/-1) BstBI 635 TTCGAA TT^CGAA BstXI 2918 CCATCATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1825 GCTCAGC GC^T[ACGT]AGC ClaI 3076 ATCGAT AT^CGAT EcoRI 4065 GAATTC G^AATTC EcoT22I 426 ATGCAT ATGCA^T FbaI 2381 TGATCA T^GATCA HindIII 3463 AAGCTT A^AGCTT KasI 1368 GGCGCC G^GCGCC KpnI 1501 GGTACC GGTAC^C NarI 1368 GGCGCC GG^CGCC NheI 3804 GCTAGC G^CTAGC NsiI 426 ATGCAT ATGCA^T NspV 635 TTCGAA TT^CGAA PaeR7I 2845 CTCGAG C^TCGAG PflFI 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 4219 CACGTG CAC^GTG PmlI 4219 CACGTG CAC^GTG PshAI 1756 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 921 ATTAAT AT^TAAT Psp1406I 1666 AACGTT AA^CGTT RcaI 768 TCATGA T^CATGA SfoI 1368 GGCGCC GGC^GCC SfuI 635 TTCGAA TT^CGAA SwaI 2051 ATTTAAAT ATTT^AAAT Tth111I 4308 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2845 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccIII 1784 TCCGGA T^CCGGA 2981 TCCGGA BalI 3903 TGGCCA TGG^CCA 3966 TGGCCA BamHI 114 GGATCC G^GATCC 943 GGATCC BcgI 3068 GCACTCTGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4101 GCATCGGAGTCG BsaI 2369 GGTCTC GGTCTC(1/5) 4418 GGTCTC BsaWI 1784 TCCGGA [AT]^CCGG[AT] 2981 TCCGGA BseAI 1784 TCCGGA T^CCGGA 2981 TCCGGA BsmBI 2708 CGTCTC CGTCTC(1/5) 3282 GAGACG Bsp1407I 318 TGTACA T^GTACA 714 TGTACA BspEI 1784 TCCGGA T^CCGGA 2981 TCCGGA BsrGI 318 TGTACA T^GTACA 714 TGTACA DsaI 4082 CCGTGG C^C[GA][CT]GG 4303 CCACGG MluNI 3903 TGGCCA TGG^CCA 3966 TGGCCA MroI 1784 TCCGGA T^CCGGA 2981 TCCGGA MscI 3903 TGGCCA TGG^CCA 3966 TGGCCA NspI 2763 ACATGC [GA]CATG^[CT] 3813 ACATGC SspBI 318 TGTACA T^GTACA 714 TGTACA XbaI 2373 TCTAGA T^CTAGA 3309 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DrdI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI