(Circular) (Six-base) MAPSORT of: pFN21AE9672.seq Check: 8321 from: 1 to: 9199 pFN21AE9672 9199 bp With 182 enzymes: SgfI * March 28, 2012 12:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4162 4459 4502 5941 4162 Size: 297 43 1439 7420 Fragments arranged by size: 7420 1439 297 43 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7009 278 Size: 53 83 186 936 5473 2468 Fragments arranged by size: 5473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6542 1937 Size: 4605 4594 Acc65I G'GTAC_C Cuts at: 3506 6520 3506 Size: 3014 6185 AclI AA'CG_TT Cuts at: 1563 3672 6498 7326 7699 1563 Size: 2109 2826 828 373 3063 Fragments arranged by size: 3063 2826 2109 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4985 5159 5648 7262 8646 8914 1632 Size: 3353 174 489 1614 1384 268 1917 Fragments arranged by size: 3353 1917 1614 1384 489 268 174 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 9002 AflIII A'CryG_T Cuts at: 1203 5436 8098 9075 1203 Size: 4233 2662 977 1327 Fragments arranged by size: 4233 2662 1327 977 AgeI A'CCGG_T Cuts at: 8030 8030 Size: 9199 AhdI GACnn_n'nnGTC Cuts at: 7928 7928 Size: 9199 AleI CACnn'nnGTG Cuts at: 1166 3084 1166 Size: 1918 7281 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3102 1184 Size: 1918 7281 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2240 2272 5778 5810 2240 Size: 32 3506 32 5629 Fragments arranged by size: 5629 3506 32 32 AlwNI CAG_nnn'CTG Cuts at: 2108 3785 3943 4045 5903 6942 8514 2108 Size: 1677 158 102 1858 1039 1572 2793 Fragments arranged by size: 2793 1858 1677 1572 1039 158 102 ApaLI G'TGCA_C Cuts at: 7256 8412 7256 Size: 1156 8043 ApoI r'AATT_y Cuts at: 1493 2637 2668 3520 4094 5030 5306 5590 6070 Size: 1144 31 852 574 936 276 284 480 Cuts at: 6070 6145 6508 6734 6886 8995 1493 Size: 75 363 226 152 2109 1697 Fragments arranged by size: 2109 1697 1144 936 852 574 480 363 284 276 226 152 75 31 AseI AT'TA_AT Cuts at: 160 1032 2927 7753 160 Size: 872 1895 4826 1606 Fragments arranged by size: 4826 1895 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9199 AvaI C'yCGr_G Cuts at: 1870 1943 2522 2622 4323 4850 6251 6524 1870 Size: 73 579 100 1701 527 1401 273 4545 Fragments arranged by size: 4545 1701 1401 579 527 273 100 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3490 3523 3490 Size: 33 9166 BamHI G'GATC_C Cuts at: 2119 2948 6529 6896 2119 Size: 829 3581 367 4422 Fragments arranged by size: 4422 3581 829 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 3506 4150 5857 6520 Size: 325 217 627 1586 133 644 1707 663 Cuts at: 6520 7975 618 Size: 1455 1842 Fragments arranged by size: 1842 1707 1586 1455 663 644 627 325 217 133 BanII G_rGCy'C Cuts at: 1391 2510 2803 3462 4851 5051 5718 6518 1391 Size: 1119 293 659 1389 200 667 800 4072 Fragments arranged by size: 4072 1389 1119 800 667 659 293 200 BbeI G_GCGC'C Cuts at: 3377 3377 Size: 9199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3671 3747 4560 5636 6174 928 Size: 971 1772 76 813 1076 538 3953 Fragments arranged by size: 3953 1772 1076 971 813 538 76 BbvCI CC'TCA_GC Cuts at: 5450 5850 6405 5450 Size: 400 555 8244 Fragments arranged by size: 8244 555 400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5062 5096 6095 6129 7390 7424 1903 Size: 34 3125 34 999 34 1261 34 3678 Fragments arranged by size: 3678 3125 1261 999 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3461 7091 8301 3461 Size: 3630 1210 4359 Fragments arranged by size: 4359 3630 1210 BclI T'GATC_A Cuts at: 1548 4386 1548 Size: 2838 6361 BfrBI ATG'CAT Cuts at: 2433 2433 Size: 9199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 5837 7810 Size: 107 122 71 631 605 337 3828 1973 Cuts at: 7810 136 Size: 1525 Fragments arranged by size: 3828 1973 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5483 9194 5483 Size: 3711 5488 BlpI GC'TnA_GC Cuts at: 3831 6616 3831 Size: 2785 6414 Bme1580I G_kGCm'C Cuts at: 1790 2962 7260 8416 1790 Size: 1172 4298 1156 2573 Fragments arranged by size: 4298 2573 1172 1156 BmgBI CAC'GTC Cuts at: 1318 4867 1318 Size: 3549 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2408 3050 3124 Size: 416 53 83 399 24 991 642 74 Cuts at: 3124 5227 6930 7888 442 Size: 2103 1703 958 1753 Fragments arranged by size: 2103 1753 1703 991 958 642 416 399 83 74 53 24 BmtI G_CTAG'C Cuts at: 1056 5813 1056 Size: 4757 4442 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5446 5732 5446 Size: 286 8913 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2223 3440 3476 3636 3788 3944 5660 5862 Size: 984 1217 36 160 152 156 1716 202 Cuts at: 5862 6432 7859 1239 Size: 570 1427 2579 Fragments arranged by size: 2579 1716 1427 1217 984 570 202 160 156 152 36 Bpu10I CC'TnA_GC Cuts at: 5450 5850 6050 6121 6405 5450 Size: 400 200 71 284 8244 Fragments arranged by size: 8244 400 284 200 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4508 4674 5702 5780 7324 8189 8487 4508 Size: 166 1028 78 1544 865 298 5220 Fragments arranged by size: 5220 1544 1028 865 298 166 78 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4380 6429 7862 882 Size: 617 2881 2049 1433 2219 Fragments arranged by size: 2881 2219 2049 1433 617 BsaAI yAC'GTr Cuts at: 493 1204 6226 6990 493 Size: 711 5022 764 2702 Fragments arranged by size: 5022 2702 764 711 BsaBI GATnn'nnATC Cuts at: 9193 9193 Size: 9199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3374 4404 5415 7006 Size: 53 83 186 936 1841 1030 1011 1591 Cuts at: 7006 7388 8023 275 Size: 382 635 1451 Fragments arranged by size: 1841 1591 1451 1030 1011 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3789 4986 7632 8030 8304 8451 8862 3789 Size: 1197 2646 398 274 147 411 4126 Fragments arranged by size: 4126 2646 1197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2871 2901 3188 3218 3281 Size: 30 165 30 1468 30 287 30 63 Cuts at: 3281 3311 4493 4523 6188 6218 6273 6303 1178 Size: 30 1182 30 1665 30 55 30 4074 Fragments arranged by size: 4074 1665 1468 1182 287 165 63 55 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4013 4177 4463 1186 Size: 2827 164 286 5922 Fragments arranged by size: 5922 2827 286 164 BseYI C'CCAG_C Cuts at: 2123 2354 4596 4708 4831 5656 8402 8898 2123 Size: 231 2242 112 123 825 2746 496 2424 Fragments arranged by size: 2746 2424 2242 825 496 231 123 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3359 3669 3866 3929 5935 6190 1163 Size: 573 1623 310 197 63 2006 255 4172 Fragments arranged by size: 4172 2006 1623 573 310 255 197 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6649 7410 8438 1540 Size: 275 186 4648 761 1028 2301 Fragments arranged by size: 4648 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2510 2803 2861 3462 3905 4322 4503 4768 4851 Size: 293 58 601 443 417 181 265 83 Cuts at: 4851 5322 5718 6056 6225 7260 7345 8416 2510 Size: 471 396 338 169 1035 85 1071 3293 Fragments arranged by size: 3293 1071 1035 601 471 443 417 396 338 293 265 181 169 85 83 58 BsmI GAATG_Cn' Cuts at: 2162 2433 4911 6710 6803 2162 Size: 271 2478 1799 93 4558 Fragments arranged by size: 4558 2478 1799 271 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4719 5281 4719 Size: 562 8637 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2510 2803 2861 2962 3462 3905 4322 Size: 399 720 293 58 101 500 443 417 Cuts at: 4322 4503 4768 4851 5051 5322 5718 6056 6225 Size: 181 265 83 200 271 396 338 169 Cuts at: 6225 6518 7260 7345 8416 1391 Size: 293 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 500 443 417 399 396 338 293 293 271 265 200 181 169 101 85 83 58 BspEI T'CCGG_A Cuts at: 3789 4986 8862 3789 Size: 1197 3876 4126 Fragments arranged by size: 4126 3876 1197 BspHI T'CATG_A Cuts at: 2773 7088 8818 2773 Size: 4315 1730 3154 Fragments arranged by size: 4315 3154 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3682 4162 4459 4502 5941 6554 844 Size: 2838 480 297 43 1439 613 3489 Fragments arranged by size: 3489 2838 1439 613 480 297 43 BsrBI CCG'CTC Cuts at: 1394 1823 7086 1394 Size: 429 5263 3507 Fragments arranged by size: 5263 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3183 3642 5097 5484 6011 6057 7694 Size: 1183 1935 459 1455 387 527 46 1637 Cuts at: 7694 7868 65 Size: 174 1396 Fragments arranged by size: 1935 1637 1455 1396 1183 527 459 387 174 46 BsrFI r'CCGG_y Cuts at: 1700 1810 7843 8030 1700 Size: 110 6033 187 2869 Fragments arranged by size: 6033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2323 2719 96 Size: 2227 396 6576 Fragments arranged by size: 6576 2227 396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9199 BssSI C'ACGA_G Cuts at: 4805 7259 8271 4805 Size: 2454 1012 5733 Fragments arranged by size: 5733 2454 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4459 5819 5873 6022 794 Size: 3665 1360 54 149 3971 Fragments arranged by size: 3971 3665 1360 149 54 BstBI TT'CG_AA Cuts at: 2641 8003 2641 Size: 5362 3837 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9199 BstXI CCAn_nnnn'nTGG Cuts at: 4930 4930 Size: 9199 BstYI r'GATC_y Cuts at: 1972 2056 2119 2213 2948 3219 3611 3849 4810 Size: 84 63 94 735 271 392 238 961 Cuts at: 4810 5483 5652 6529 6896 7281 7298 8750 8836 Size: 673 169 877 367 385 17 1452 86 Cuts at: 8836 8848 9194 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 961 877 735 673 392 385 367 346 271 238 169 94 86 84 63 17 12 Bsu36I CC'TnA_GG Cuts at: 3571 4267 4582 4783 3571 Size: 696 315 201 7987 Fragments arranged by size: 7987 696 315 201 BtgI C'CryG_G Cuts at: 513 1065 2004 6087 6308 513 Size: 552 939 4083 221 3404 Fragments arranged by size: 4083 3404 939 552 221 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8460 Fragments arranged by size: 8460 683 56 BtsI GCAGTG_nn' Cuts at: 2395 3601 3686 3786 4705 6377 6416 6715 7509 Size: 1206 85 100 919 1672 39 299 794 Cuts at: 7509 7529 2395 Size: 20 4065 Fragments arranged by size: 4065 1672 1206 919 794 299 100 85 39 20 ClaI AT'CG_AT Cuts at: 1554 5082 1554 Size: 3528 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9164 DraI TTT'AAA Cuts at: 2664 4059 6144 6503 2664 Size: 1395 2085 359 5360 Fragments arranged by size: 5360 2085 1395 359 DraIII CAC_nnn'GTG Cuts at: 2085 2416 2717 2085 Size: 331 301 8567 Fragments arranged by size: 8567 331 301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3995 4871 6319 8206 809 Size: 507 2679 876 1448 1887 1802 Fragments arranged by size: 2679 1887 1802 1448 876 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5829 5908 5971 Size: 54 1425 50 275 18 3999 79 63 Cuts at: 5971 6646 7535 9164 8 Size: 675 889 1629 43 Fragments arranged by size: 3999 1629 1425 889 675 275 79 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6646 1537 Size: 275 4834 4090 Fragments arranged by size: 4834 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 4817 5135 5579 6251 7129 1353 Size: 1091 2373 318 444 672 878 3423 Fragments arranged by size: 3423 2373 1091 878 672 444 318 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7786 8160 8306 387 Size: 1064 138 6197 374 146 1280 Fragments arranged by size: 6197 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2508 2801 3460 4849 5716 2508 Size: 293 659 1389 867 5991 Fragments arranged by size: 5991 1389 867 659 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 3440 3476 3636 3788 3944 4985 Size: 393 591 1217 36 160 152 156 1041 Cuts at: 4985 5159 5648 5660 5862 6432 7262 7859 8646 Size: 174 489 12 202 570 830 597 787 Cuts at: 8646 8914 1239 Size: 268 1524 Fragments arranged by size: 1524 1217 1041 830 787 597 591 570 489 393 268 202 174 160 156 152 36 12 EcoNI CCTnn'n_nnAGG Cuts at: 3338 3541 4192 4411 6003 6457 3338 Size: 203 651 219 1592 454 6080 Fragments arranged by size: 6080 1592 651 454 219 203 EcoO109I rG'GnC_Cy Cuts at: 2020 2626 3772 3799 3919 3997 4705 5446 6465 Size: 606 1146 27 120 78 708 741 1019 Cuts at: 6465 2020 Size: 4754 Fragments arranged by size: 4754 1146 1019 741 708 606 120 78 27 EcoRI G'AATT_C Cuts at: 6070 6508 6886 6070 Size: 438 378 8383 Fragments arranged by size: 8383 438 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2713 4645 4813 5522 5743 2713 Size: 1932 168 709 221 6169 Fragments arranged by size: 6169 1932 709 221 168 FspI TGC'GCA Cuts at: 7705 7705 Size: 9199 HaeII r_GCGC'y Cuts at: 1678 3024 3377 4013 8346 1678 Size: 1346 353 636 4333 2531 Fragments arranged by size: 4333 2531 1346 636 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2871 2903 3457 3489 4263 Size: 32 135 32 1299 32 554 32 774 Cuts at: 4263 4295 4472 4504 4728 4760 5331 5363 6091 Size: 32 177 32 224 32 571 32 728 Cuts at: 6091 6123 6188 6220 6273 6305 7840 7872 7914 Size: 32 65 32 53 32 1535 32 42 Cuts at: 7914 7946 1373 Size: 32 2626 Fragments arranged by size: 2626 1535 1299 774 728 571 554 224 177 135 65 53 42 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2077 3426 4402 5737 6543 6789 Size: 988 281 139 1349 976 1335 806 246 Cuts at: 6789 669 Size: 3079 Fragments arranged by size: 3079 1349 1335 988 976 806 281 246 139 HindIII A'AGCT_T Cuts at: 748 5468 748 Size: 4720 4479 HpaI GTT'AAC Cuts at: 6789 6789 Size: 9199 KasI G'GCGC_C Cuts at: 3373 3373 Size: 9199 KpnI G_GTAC'C Cuts at: 3510 6524 3510 Size: 3014 6185 MfeI C'AATT_G Cuts at: 6798 6798 Size: 9199 MluI A'CGCG_T Cuts at: 9075 9075 Size: 9199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3423 3589 4536 4998 5188 6095 8313 8497 Size: 2118 166 947 462 190 907 2218 184 Cuts at: 8497 1305 Size: 2007 Fragments arranged by size: 2218 2118 2007 947 907 462 190 184 166 MscI TGG'CCA Cuts at: 10 64 1489 1832 5910 5973 10 Size: 54 1425 343 4078 63 3236 Fragments arranged by size: 4078 3236 1425 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 3084 3635 3720 4895 4925 4928 4955 Size: 648 1918 551 85 1175 30 3 27 Cuts at: 4955 5823 7157 7516 7675 518 Size: 868 1334 359 159 2042 Fragments arranged by size: 2042 1918 1334 1175 868 648 551 359 159 85 30 27 3 MspA1I CmG'CkG Cuts at: 1606 2053 2354 3145 3940 4397 4600 5746 5791 Size: 447 301 791 795 457 203 1146 45 Cuts at: 5791 6615 7292 8440 8685 1606 Size: 824 677 1148 245 2120 Fragments arranged by size: 2120 1148 1146 824 795 791 677 457 447 301 245 203 45 NarI GG'CG_CC Cuts at: 3374 3374 Size: 9199 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 7708 Fragments arranged by size: 7708 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 9199 NheI G'CTAG_C Cuts at: 1052 5809 1052 Size: 4757 4442 NotI GC'GGCC_GC Cuts at: 6646 6646 Size: 9199 NsiI A_TGCA'T Cuts at: 2435 2435 Size: 9199 NspI r_CATG'y Cuts at: 1132 1229 4772 5822 6557 8102 1132 Size: 97 3543 1050 735 1545 2229 Fragments arranged by size: 3543 2229 1545 1050 735 97 PasI CC'CwG_GG Cuts at: 1348 5958 1348 Size: 4610 4589 PciI A'CATG_T Cuts at: 8098 8098 Size: 9199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 9199 PfoI T'CCnGG_A Cuts at: 3453 3930 5796 3453 Size: 477 1866 6856 Fragments arranged by size: 6856 1866 477 PmeI GTTT'AAAC Cuts at: 6503 6503 Size: 9199 PmlI CAC'GTG Cuts at: 6226 6226 Size: 9199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7268 7300 8781 8813 7268 Size: 32 1481 32 7654 Fragments arranged by size: 7654 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2626 3772 3997 5446 6465 2626 Size: 1146 225 1449 1019 5360 Fragments arranged by size: 5360 1449 1146 1019 225 PshAI GACnn'nnGTC Cuts at: 1613 3765 1613 Size: 2152 7047 PsiI TTA'TAA Cuts at: 4112 6769 4112 Size: 2657 6542 PspXI vC'TCGA_Gb Cuts at: 4850 4850 Size: 9199 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5210 5242 5210 Size: 32 9167 PstI C_TGCA'G Cuts at: 830 2040 3078 3693 3759 3781 3951 4435 6458 Size: 1210 1038 615 66 22 170 484 2023 Cuts at: 6458 6551 830 Size: 93 3478 Fragments arranged by size: 3478 2023 1210 1038 615 484 170 93 66 22 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9199 PvuII CAG'CTG Cuts at: 3145 3940 4397 4600 5746 5791 3145 Size: 795 457 203 1146 45 6553 Fragments arranged by size: 6553 1146 795 457 203 45 SacI G_AGCT'C Cuts at: 2510 2803 3462 4851 5718 2510 Size: 293 659 1389 867 5991 Fragments arranged by size: 5991 1389 867 659 293 SalI G'TCGA_C Cuts at: 1936 6541 1936 Size: 4605 4594 SanDI GG'GwC_CC Cuts at: 3772 3772 Size: 9199 SapI GCTCTTCn'nnn_ Cuts at: 2444 4817 6251 2444 Size: 2373 1434 5392 Fragments arranged by size: 5392 2373 1434 SbfI CC_TGCA'GG Cuts at: 6551 6551 Size: 9199 ScaI AGT'ACT Cuts at: 7447 9159 7447 Size: 1712 7487 SfcI C'TryA_G Cuts at: 826 1045 2036 2992 3074 3689 3755 3777 3947 Size: 219 991 956 82 615 66 22 170 Cuts at: 3947 4204 4431 6454 6547 7682 8363 8554 826 Size: 257 227 2023 93 1135 681 191 1471 Fragments arranged by size: 2023 1471 1135 991 956 681 615 257 227 219 191 170 93 82 66 22 SfoI GGC'GCC Cuts at: 3375 3375 Size: 9199 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9199 SmaI CCC'GGG Cuts at: 1872 2524 2624 4325 6526 1872 Size: 652 100 1701 2201 4545 Fragments arranged by size: 4545 2201 1701 652 100 SmlI C'TyrA_G Cuts at: 820 1017 1943 4523 4689 4850 5717 5759 7303 Size: 197 926 2580 166 161 867 42 1544 Cuts at: 7303 8204 8466 820 Size: 901 262 1553 Fragments arranged by size: 2580 1553 1544 926 901 867 262 197 166 161 42 SnaBI TAC'GTA Cuts at: 493 6990 493 Size: 6497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9199 SphI G_CATG'C Cuts at: 1132 6557 1132 Size: 5425 3774 SspI AAT'ATT Cuts at: 5 52 2608 2925 5605 7123 5 Size: 47 2556 317 2680 1518 2081 Fragments arranged by size: 2680 2556 2081 1518 317 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9199 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 3090 3300 5529 6638 513 Size: 552 939 19 1067 210 2229 1109 3074 Fragments arranged by size: 3074 2229 1109 1067 939 552 210 19 SwaI ATTT'AAAT Cuts at: 4059 4059 Size: 9199 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2427 2566 4117 4207 5106 5971 7226 Size: 1255 847 139 1551 90 899 865 1255 Cuts at: 7226 7243 7396 7581 8026 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1551 1498 1255 1255 899 865 847 445 185 153 139 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2264 2323 2719 Size: 274 80 33 51 1443 287 59 396 Cuts at: 2719 4483 4569 5217 7445 9157 96 Size: 1764 86 648 2228 1712 138 Fragments arranged by size: 2228 1764 1712 1443 648 396 287 274 138 86 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3731 3763 4493 4525 9159 9191 3731 Size: 32 730 32 4634 32 3739 Fragments arranged by size: 4634 3739 730 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6316 1316 Size: 5000 4199 XbaI T'CTAG_A Cuts at: 4378 5314 6535 4378 Size: 936 1221 7042 Fragments arranged by size: 7042 1221 936 XhoI C'TCGA_G Cuts at: 1943 4850 1943 Size: 2907 6292 XmaI C'CCGG_G Cuts at: 1870 2522 2622 4323 6524 1870 Size: 652 100 1701 2201 4545 Fragments arranged by size: 4545 2201 1701 652 100 XmnI GAAnn'nnTTC Cuts at: 7328 7328 Size: 9199 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7007 276 Size: 53 83 186 936 5473 2468 Fragments arranged by size: 5473 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI RsrII SacII SexAI SfiI SrfI XcmI