(Linear) (Six-base) MAPSORT of: KIEE9672.seq Check: 9527 from: 1 to: 4494 KIEE9672.orf With 182 enzymes: SgfI * March 22, 2012 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2157 2454 2497 3936 4494 Size: 2157 297 43 1439 558 Fragments arranged by size: 2157 1439 558 297 43 Acc65I G'GTAC_C Cuts at: 0 1501 4494 Size: 1501 2993 AclI AA'CG_TT Cuts at: 0 1667 4494 Size: 1667 2827 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2980 3154 3643 4494 Size: 2980 174 489 851 Fragments arranged by size: 2980 851 489 174 AflIII A'CryG_T Cuts at: 0 3431 4494 Size: 3431 1063 AleI CACnn'nnGTG Cuts at: 0 1079 4494 Size: 1079 3415 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1097 4494 Size: 1097 3397 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 235 267 3773 3805 4494 Size: 235 32 3506 32 689 Fragments arranged by size: 3506 689 235 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 103 1780 1938 2040 3898 4494 Size: 103 1677 158 102 1858 596 Fragments arranged by size: 1858 1677 596 158 103 102 ApoI r'AATT_y Cuts at: 0 632 663 1515 2089 3025 3301 3585 4065 Size: 632 31 852 574 936 276 284 480 Cuts at: 4065 4140 4494 Size: 75 354 Fragments arranged by size: 936 852 632 574 480 354 284 276 75 31 AseI AT'TA_AT Cuts at: 0 922 4494 Size: 922 3572 AvaI C'yCGr_G Cuts at: 0 517 617 2318 2845 4246 4494 Size: 517 100 1701 527 1401 248 Fragments arranged by size: 1701 1401 527 517 248 100 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1485 1518 4494 Size: 1485 33 2976 Fragments arranged by size: 2976 1485 33 BamHI G'GATC_C Cuts at: 0 114 943 4494 Size: 114 829 3551 Fragments arranged by size: 3551 829 114 BanI G'GyrC_C Cuts at: 0 1368 1501 2145 3852 4494 Size: 1368 133 644 1707 642 Fragments arranged by size: 1707 1368 644 642 133 BanII G_rGCy'C Cuts at: 0 505 798 1457 2846 3046 3713 4494 Size: 505 293 659 1389 200 667 781 Fragments arranged by size: 1389 781 667 659 505 293 200 BbeI G_GCGC'C Cuts at: 0 1372 4494 Size: 1372 3122 BbsI GAAGACnn'nnnn_ Cuts at: 0 1666 1742 2555 3631 4169 4494 Size: 1666 76 813 1076 538 325 Fragments arranged by size: 1666 1076 813 538 325 76 BbvCI CC'TCA_GC Cuts at: 0 3445 3845 4400 4494 Size: 3445 400 555 94 Fragments arranged by size: 3445 555 400 94 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3057 3091 4090 4124 4494 Size: 3057 34 999 34 370 Fragments arranged by size: 3057 999 370 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1456 4494 Size: 1456 3038 BclI T'GATC_A Cuts at: 0 2381 4494 Size: 2381 2113 BfrBI ATG'CAT Cuts at: 0 428 4494 Size: 428 4066 BglI GCCn_nnn'nGGC Cuts at: 0 3832 4494 Size: 3832 662 BglII A'GATC_T Cuts at: 0 3478 4494 Size: 3478 1016 BlpI GC'TnA_GC Cuts at: 0 1826 4494 Size: 1826 2668 Bme1580I G_kGCm'C Cuts at: 0 957 4494 Size: 957 3537 BmgBI CAC'GTC Cuts at: 0 2862 4494 Size: 2862 1632 BmrI ACTGGGnnnn_n' Cuts at: 0 403 1045 1119 3222 4494 Size: 403 642 74 2103 1272 Fragments arranged by size: 2103 1272 642 403 74 BmtI G_CTAG'C Cuts at: 0 3808 4494 Size: 3808 686 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 3441 3727 4494 Size: 3441 286 767 Fragments arranged by size: 3441 767 286 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 1435 1471 1631 1783 1939 3655 3857 Size: 218 1217 36 160 152 156 1716 202 Cuts at: 3857 4427 4494 Size: 570 67 Fragments arranged by size: 1716 1217 570 218 202 160 156 152 67 36 Bpu10I CC'TnA_GC Cuts at: 0 3445 3845 4045 4116 4400 4494 Size: 3445 400 200 71 284 94 Fragments arranged by size: 3445 400 284 200 94 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2503 2669 3697 3775 4494 Size: 2503 166 1028 78 719 Fragments arranged by size: 2503 1028 719 166 78 BsaI GGTCTCn'nnnn_ Cuts at: 0 2375 4424 4494 Size: 2375 2049 70 Fragments arranged by size: 2375 2049 70 BsaAI yAC'GTr Cuts at: 0 4221 4494 Size: 4221 273 BsaHI Gr'CG_yC Cuts at: 0 1369 2399 3410 4494 Size: 1369 1030 1011 1084 Fragments arranged by size: 1369 1084 1030 1011 BsaWI w'CCGG_w Cuts at: 0 1784 2981 4494 Size: 1784 1197 1513 Fragments arranged by size: 1784 1513 1197 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 866 896 1183 1213 1276 1306 2488 2518 Size: 866 30 287 30 63 30 1182 30 Cuts at: 2518 4183 4213 4268 4298 4494 Size: 1665 30 55 30 196 Fragments arranged by size: 1665 1182 866 287 196 63 55 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 2008 2172 2458 4494 Size: 2008 164 286 2036 Fragments arranged by size: 2036 2008 286 164 BseYI C'CCAG_C Cuts at: 0 118 349 2591 2703 2826 3651 4494 Size: 118 231 2242 112 123 825 843 Fragments arranged by size: 2242 843 825 231 123 118 112 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1354 1664 1861 1924 3930 4185 4494 Size: 1354 310 197 63 2006 255 309 Fragments arranged by size: 2006 1354 310 309 255 197 63 BsiHKAI G_wGCw'C Cuts at: 0 505 798 856 1457 1900 2317 2498 2763 Size: 505 293 58 601 443 417 181 265 Cuts at: 2763 2846 3317 3713 4051 4220 4494 Size: 83 471 396 338 169 274 Fragments arranged by size: 601 505 471 443 417 396 338 293 274 265 181 169 83 58 BsmI GAATG_Cn' Cuts at: 0 157 428 2906 4494 Size: 157 271 2478 1588 Fragments arranged by size: 2478 1588 271 157 BsmBI CGTCTCn'nnnn_ Cuts at: 0 2714 3276 4494 Size: 2714 562 1218 Fragments arranged by size: 2714 1218 562 Bsp1286I G_dGCh'C Cuts at: 0 505 798 856 957 1457 1900 2317 2498 Size: 505 293 58 101 500 443 417 181 Cuts at: 2498 2763 2846 3046 3317 3713 4051 4220 4494 Size: 265 83 200 271 396 338 169 274 Fragments arranged by size: 505 500 443 417 396 338 293 274 271 265 200 181 169 101 83 58 BspEI T'CCGG_A Cuts at: 0 1784 2981 4494 Size: 1784 1197 1513 Fragments arranged by size: 1784 1513 1197 BspHI T'CATG_A Cuts at: 0 768 4494 Size: 768 3726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1677 2157 2454 2497 3936 4494 Size: 1677 480 297 43 1439 558 Fragments arranged by size: 1677 1439 558 480 297 43 BsrDI GCAATG_nn' Cuts at: 0 1178 1637 3092 3479 4006 4052 4494 Size: 1178 459 1455 387 527 46 442 Fragments arranged by size: 1455 1178 527 459 442 387 46 BsrGI T'GTAC_A Cuts at: 0 318 714 4494 Size: 318 396 3780 Fragments arranged by size: 3780 396 318 BssSI C'ACGA_G Cuts at: 0 2800 4494 Size: 2800 1694 BstAPI GCAn_nnn'nTGC Cuts at: 0 2454 3814 3868 4017 4494 Size: 2454 1360 54 149 477 Fragments arranged by size: 2454 1360 477 149 54 BstBI TT'CG_AA Cuts at: 0 636 4494 Size: 636 3858 BstXI CCAn_nnnn'nTGG Cuts at: 0 2925 4494 Size: 2925 1569 BstYI r'GATC_y Cuts at: 0 51 114 208 943 1214 1606 1844 2805 Size: 51 63 94 735 271 392 238 961 Cuts at: 2805 3478 3647 4494 Size: 673 169 847 Fragments arranged by size: 961 847 735 673 392 271 238 169 94 63 51 Bsu36I CC'TnA_GG Cuts at: 0 1566 2262 2577 2778 4494 Size: 1566 696 315 201 1716 Fragments arranged by size: 1716 1566 696 315 201 BtgI C'CryG_G Cuts at: 0 4082 4303 4494 Size: 4082 221 191 Fragments arranged by size: 4082 221 191 BtsI GCAGTG_nn' Cuts at: 0 390 1596 1681 1781 2700 4372 4411 4494 Size: 390 1206 85 100 919 1672 39 83 Fragments arranged by size: 1672 1206 919 390 100 85 83 39 ClaI AT'CG_AT Cuts at: 0 3077 4494 Size: 3077 1417 DraI TTT'AAA Cuts at: 0 659 2054 4139 4494 Size: 659 1395 2085 355 Fragments arranged by size: 2085 1395 659 355 DraIII CAC_nnn'GTG Cuts at: 0 80 411 712 4494 Size: 80 331 301 3782 Fragments arranged by size: 3782 331 301 80 DrdI GACnn_nn'nnGTC Cuts at: 0 1990 2866 4314 4494 Size: 1990 876 1448 180 Fragments arranged by size: 1990 1448 876 180 EaeI y'GGCC_r Cuts at: 0 3824 3903 3966 4494 Size: 3824 79 63 528 Fragments arranged by size: 3824 528 79 63 EarI CTCTTCn'nnn_ Cuts at: 0 439 2812 3130 3574 4246 4494 Size: 439 2373 318 444 672 248 Fragments arranged by size: 2373 672 444 439 318 248 EcoICRI GAG'CTC Cuts at: 0 503 796 1455 2844 3711 4494 Size: 503 293 659 1389 867 783 Fragments arranged by size: 1389 867 783 659 503 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 218 1435 1471 1631 1783 1939 2980 3154 Size: 218 1217 36 160 152 156 1041 174 Cuts at: 3154 3643 3655 3857 4427 4494 Size: 489 12 202 570 67 Fragments arranged by size: 1217 1041 570 489 218 202 174 160 156 152 67 36 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1333 1536 2187 2406 3998 4452 4494 Size: 1333 203 651 219 1592 454 42 Fragments arranged by size: 1592 1333 651 454 219 203 42 EcoO109I rG'GnC_Cy Cuts at: 0 15 621 1767 1794 1914 1992 2700 3441 Size: 15 606 1146 27 120 78 708 741 Cuts at: 3441 4460 4494 Size: 1019 34 Fragments arranged by size: 1146 1019 741 708 606 120 78 34 27 15 EcoRI G'AATT_C Cuts at: 0 4065 4494 Size: 4065 429 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 708 2640 2808 3517 3738 4494 Size: 708 1932 168 709 221 756 Fragments arranged by size: 1932 756 709 708 221 168 HaeII r_GCGC'y Cuts at: 0 1019 1372 2008 4494 Size: 1019 353 636 2486 Fragments arranged by size: 2486 1019 636 353 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 866 898 1452 1484 2258 2290 2467 2499 Size: 866 32 554 32 774 32 177 32 Cuts at: 2499 2723 2755 3326 3358 4086 4118 4183 4215 Size: 224 32 571 32 728 32 65 32 Cuts at: 4215 4268 4300 4494 Size: 53 32 194 Fragments arranged by size: 866 774 728 571 554 224 194 177 65 53 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 72 1421 2397 3732 4494 Size: 72 1349 976 1335 762 Fragments arranged by size: 1349 1335 976 762 72 HindIII A'AGCT_T Cuts at: 0 3463 4494 Size: 3463 1031 KasI G'GCGC_C Cuts at: 0 1368 4494 Size: 1368 3126 KpnI G_GTAC'C Cuts at: 0 1505 4494 Size: 1505 2989 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1418 1584 2531 2993 3183 4090 4494 Size: 1418 166 947 462 190 907 404 Fragments arranged by size: 1418 947 907 462 404 190 166 MscI TGG'CCA Cuts at: 0 3905 3968 4494 Size: 3905 63 526 Fragments arranged by size: 3905 526 63 MslI CAynn'nnrTG Cuts at: 0 1079 1630 1715 2890 2920 2923 2950 3818 Size: 1079 551 85 1175 30 3 27 868 Cuts at: 3818 4494 Size: 676 Fragments arranged by size: 1175 1079 868 676 551 85 30 27 3 MspA1I CmG'CkG Cuts at: 0 48 349 1140 1935 2392 2595 3741 3786 Size: 48 301 791 795 457 203 1146 45 Cuts at: 3786 4494 Size: 708 Fragments arranged by size: 1146 795 791 708 457 301 203 48 45 NarI GG'CG_CC Cuts at: 0 1369 4494 Size: 1369 3125 NheI G'CTAG_C Cuts at: 0 3804 4494 Size: 3804 690 NsiI A_TGCA'T Cuts at: 0 430 4494 Size: 430 4064 NspI r_CATG'y Cuts at: 0 2767 3817 4494 Size: 2767 1050 677 Fragments arranged by size: 2767 1050 677 PasI CC'CwG_GG Cuts at: 0 3953 4494 Size: 3953 541 PfoI T'CCnGG_A Cuts at: 0 1448 1925 3791 4494 Size: 1448 477 1866 703 Fragments arranged by size: 1866 1448 703 477 PmlI CAC'GTG Cuts at: 0 4221 4494 Size: 4221 273 PpuMI rG'GwC_Cy Cuts at: 0 621 1767 1992 3441 4460 4494 Size: 621 1146 225 1449 1019 34 Fragments arranged by size: 1449 1146 1019 621 225 34 PshAI GACnn'nnGTC Cuts at: 0 1760 4494 Size: 1760 2734 PsiI TTA'TAA Cuts at: 0 2107 4494 Size: 2107 2387 PspXI vC'TCGA_Gb Cuts at: 0 2845 4494 Size: 2845 1649 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 3205 3237 4494 Size: 3205 32 1257 Fragments arranged by size: 3205 1257 32 PstI C_TGCA'G Cuts at: 0 35 1073 1688 1754 1776 1946 2430 4453 Size: 35 1038 615 66 22 170 484 2023 Cuts at: 4453 4494 Size: 41 Fragments arranged by size: 2023 1038 615 484 170 66 41 35 22 PvuII CAG'CTG Cuts at: 0 1140 1935 2392 2595 3741 3786 4494 Size: 1140 795 457 203 1146 45 708 Fragments arranged by size: 1146 1140 795 708 457 203 45 SacI G_AGCT'C Cuts at: 0 505 798 1457 2846 3713 4494 Size: 505 293 659 1389 867 781 Fragments arranged by size: 1389 867 781 659 505 293 SanDI GG'GwC_CC Cuts at: 0 1767 4494 Size: 1767 2727 SapI GCTCTTCn'nnn_ Cuts at: 0 439 2812 4246 4494 Size: 439 2373 1434 248 Fragments arranged by size: 2373 1434 439 248 SfcI C'TryA_G Cuts at: 0 31 987 1069 1684 1750 1772 1942 2199 Size: 31 956 82 615 66 22 170 257 Cuts at: 2199 2426 4449 4494 Size: 227 2023 45 Fragments arranged by size: 2023 956 615 257 227 170 82 66 45 31 22 SfoI GGC'GCC Cuts at: 0 1370 4494 Size: 1370 3124 SmaI CCC'GGG Cuts at: 0 519 619 2320 4494 Size: 519 100 1701 2174 Fragments arranged by size: 2174 1701 519 100 SmlI C'TyrA_G Cuts at: 0 2518 2684 2845 3712 3754 4494 Size: 2518 166 161 867 42 740 Fragments arranged by size: 2518 867 740 166 161 42 SspI AAT'ATT Cuts at: 0 603 920 3600 4494 Size: 603 317 2680 894 Fragments arranged by size: 2680 894 603 317 StyI C'CwwG_G Cuts at: 0 18 1085 1295 3524 4494 Size: 18 1067 210 2229 970 Fragments arranged by size: 2229 1067 970 210 18 SwaI ATTT'AAAT Cuts at: 0 2054 4494 Size: 2054 2440 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 422 561 2112 2202 3101 3966 4494 Size: 422 139 1551 90 899 865 528 Fragments arranged by size: 1551 899 865 528 422 139 90 TatI w'GTAC_w Cuts at: 0 259 318 714 2478 2564 3212 4494 Size: 259 59 396 1764 86 648 1282 Fragments arranged by size: 1764 1282 648 396 259 86 59 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1726 1758 2488 2520 4494 Size: 1726 32 730 32 1974 Fragments arranged by size: 1974 1726 730 32 32 Tth111I GACn'n_nGTC Cuts at: 0 4311 4494 Size: 4311 183 XbaI T'CTAG_A Cuts at: 0 2373 3309 4494 Size: 2373 936 1185 Fragments arranged by size: 2373 1185 936 XhoI C'TCGA_G Cuts at: 0 2845 4494 Size: 2845 1649 XmaI C'CCGG_G Cuts at: 0 517 617 2318 4494 Size: 517 100 1701 2176 Fragments arranged by size: 2176 1701 517 100 Enzymes that do cut: AarI Acc65I AclI AcuI AflIII AleI AlfI AloI AlwNI ApoI AseI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MmeI MscI MslI MspA1I NarI NheI NsiI NspI PasI PfoI PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuII SacI SanDI SapI SfcI SfoI SmaI SmlI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI Enzymes that do not cut: AatII AccI AfeI AflII AgeI AhdI ApaI ApaLI AscI AsiSI AvrII BsaBI BsiEI BsiWI BsrBI BsrFI BssHII BstEII BstZ17I BtgZI CspCI EagI EciI EcoRV FseI FspI FspAI HpaI MfeI MluI NaeI NcoI NdeI NgoMIV NotI NruI PacI PciI PflMI PmeI PpiI PspOMI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI StuI XcmI XmnI ZraI