################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AviII AvrII BanI BbeI BcgI BfrI BglII BlnI BlpI Bpu1102I BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I FseI FspI HpaI KasI KpnI KspI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspBI SspI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 1191 GACGTC GACGT^C AccII 1809 CGCG CG^CG Alw44I 1049 GTGCAC G^TGCAC AlwNI 417 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 1475 AGCGCT AGC^GCT ApaLI 1049 GTGCAC G^TGCAC Asp700I 1905 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 1538 CTCGAG C^[CT]CG[GA]G BamHI 216 GGATCC G^GATCC BbrPI 1164 CACGTG CAC^GTG BbvCI 1259 CCTCAGC CCTCAGC(-5/-2) BciVI 2126 GGATAC GTATCC(6/5) BclI 2093 TGATCA T^GATCA BcnI 373 CCGGG CC^[GC]GG BfaI 494 CTAG C^TAG BglI 737 GCCACCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1164 CACGTG [CT]AC^GT[GA] BseRI 19 CTCCTC GAGGAG(10/8) BsiEI 1103 CGACCG CG[GA][CT]^CG BsmBI 1189 GAGACG CGTCTC(1/5) BsoBI 1538 CTCGAG C^[CT]CG[GA]G BstUI 1809 CGCG CG^CG DraI 1467 TTTAAA TTT^AAA DsaI 452 CCATGG C^C[GA][CT]GG Eco47III 1475 AGCGCT AGC^GCT EcoNI 2263 CCTGTGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1533 GAATTC G^AATTC EcoRV 394 GATATC GAT^ATC EcoT22I 2017 ATGCAT ATGCA^T FauI 1810 GCGGG CCCGC(4/6) FbaI 2093 TGATCA T^GATCA HaeII 1475 AGCGCT [GA]GCGC^[CT] HincII 445 GTCAAC GT[CT]^[GA]AC HindII 445 GTCAAC GT[CT]^[GA]AC HindIII 1712 AAGCTT A^AGCTT MaeI 494 CTAG C^TAG MfeI 1397 CAATTG C^AATTG MunI 1397 CAATTG C^AATTG MvnI 1809 CGCG CG^CG NciI 373 CCGGG CC^[GC]GG NcoI 452 CCATGG C^CATGG NdeI 1034 CATATG CA^TATG NsiI 2017 ATGCAT ATGCA^T PaeR7I 1538 CTCGAG C^TCGAG PleI 1541 GAGTC GAGTC(4/5) PmaCI 1164 CACGTG CAC^GTG PmlI 1164 CACGTG CAC^GTG PpuMI 359 GGGTCCC [GA]G^G[AT]CC[CT] SacI 1783 GAGCTC GAGCT^C SapI 2214 GCTCTTC GCTCTTC(1/4) SbfI 2307 CCTGCAGG CCTGCA^GG SexAI 2182 ACCAGGT A^CC[AT]GGT SphI 552 GCATGC GCATG^C Sse8387I 2307 CCTGCAGG CCTGCA^GG StuI 2201 AGGCCT AGG^CCT TaqI 1539 TCGA T^CGA Tsp45I 1345 GTGAC ^GT[GC]AC TthHB8I 1539 TCGA T^CGA XbaI 493 TCTAGA T^CTAGA XcmI 1205 CCACAGTCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1538 CTCGAG C^TCGAG XmnI 1905 GAATTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AcyI 1191 GACGTC G[GA]^CG[CT]C 1773 GGCGTC AspI 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 439 GACTGTGTC BalI 621 TGGCCA TGG^CCA 1202 TGGCCA BanII 867 GGGCTC G[GA]GC[CT]^C 1783 GAGCTC BsaHI 1191 GACGTC G[GA]^CG[CT]C 1773 GGCGTC BsaI 1420 GAGACC GGTCTC(1/5) 640 GGTCTC BstAPI 416 GCAGCGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1753 GCAAATCTTGC DraII 359 GGGTCCC [GA]G^G[ACGT]CC[CT] 2314 GGGGCCT EcoO109I 359 GGGTCCC [GA]G^G[ACGT]CC[CT] 2314 GGGGCCT HapII 373 CCGG C^CGG 1276 CCGG HgaI 419 GCGTC GACGC(5/10) 1774 GCGTC Hin1I 1191 GACGTC G[GA]^CG[CT]C 1773 GGCGTC HpaII 373 CCGG C^CGG 1276 CCGG MluNI 621 TGGCCA TGG^CCA 1202 TGGCCA MscI 621 TGGCCA TGG^CCA 1202 TGGCCA MspI 373 CCGG C^CGG 1276 CCGG NspI 552 GCATGC [GA]CATG^[CT] 700 ACATGC PflFI 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 439 GACTGTGTC PflMI 754 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 783 CCAAGGAGTGG PstI 1734 CTGCAG CTGCA^G 2308 CTGCAG Tth111I 355 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 439 GACTGTGTC Van91I 754 CCAAGCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 783 CCAAGGAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BmyI BpmI BpuAI BsaBI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI Ksp632I MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp509I TspRI XhoII