(Circular) (Six-base) MAPSORT of: pFN21AE9451.seq Check: 9757 from: 1 to: 7168 pFN21AE9451 7168 bp With 182 enzymes: SgfI * March 28, 2012 12:11 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2747 4265 2747 Size: 1518 5650 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3200 4978 278 Size: 53 83 186 936 1664 1778 2468 Fragments arranged by size: 2468 1778 1664 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4511 1937 Size: 2574 4594 Acc65I G'GTAC_C Cuts at: 4489 4489 Size: 7168 AclI AA'CG_TT Cuts at: 1563 5295 5668 1563 Size: 3732 373 3063 Fragments arranged by size: 3732 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2926 3058 3143 3551 3901 3920 5231 6615 Size: 1294 132 85 408 350 19 1311 1384 Cuts at: 6615 6883 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1311 1294 408 350 268 132 85 19 AfeI AGC'GCT Cuts at: 3482 3482 Size: 7168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6971 AflIII A'CryG_T Cuts at: 1203 6067 7044 1203 Size: 4864 977 1327 Fragments arranged by size: 4864 1327 977 AgeI A'CCGG_T Cuts at: 5999 5999 Size: 7168 AhdI GACnn_n'nnGTC Cuts at: 5897 5897 Size: 7168 AleI CACnn'nnGTG Cuts at: 1166 3434 3671 3948 1166 Size: 2268 237 277 4386 Fragments arranged by size: 4386 2268 277 237 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3966 1184 Size: 2782 4386 AlwNI CAG_nnn'CTG Cuts at: 2427 4911 6483 2427 Size: 2484 1572 3112 Fragments arranged by size: 3112 2484 1572 ApaLI G'TGCA_C Cuts at: 3054 5225 6381 3054 Size: 2171 1156 3841 Fragments arranged by size: 3841 2171 1156 ApoI r'AATT_y Cuts at: 1493 2776 3179 3538 4477 4703 4855 6964 1493 Size: 1283 403 359 939 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 939 403 359 226 152 AseI AT'TA_AT Cuts at: 160 1032 5722 160 Size: 872 4690 1606 Fragments arranged by size: 4690 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 AvaI C'yCGr_G Cuts at: 1870 1943 3543 4493 1870 Size: 73 1600 950 4545 Fragments arranged by size: 4545 1600 950 73 BamHI G'GATC_C Cuts at: 2221 4498 4865 2221 Size: 2277 367 4524 Fragments arranged by size: 4524 2277 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4489 5944 618 Size: 325 217 627 2702 1455 1842 Fragments arranged by size: 2702 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2876 3792 4487 1391 Size: 1485 916 695 4072 Fragments arranged by size: 4072 1485 916 695 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2523 3208 4400 928 Size: 971 624 685 1192 3696 Fragments arranged by size: 3696 1192 971 685 624 BbvCI CC'TCA_GC Cuts at: 3265 3265 Size: 7168 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5359 5393 1903 Size: 34 50 34 3338 34 3678 Fragments arranged by size: 3678 3338 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4125 5060 6270 4125 Size: 935 1210 5023 Fragments arranged by size: 5023 1210 935 BclI T'GATC_A Cuts at: 1548 4098 1548 Size: 2550 4618 BfrBI ATG'CAT Cuts at: 4024 4024 Size: 7168 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2748 5779 136 Size: 107 122 71 631 605 1076 3031 1525 Fragments arranged by size: 3031 1525 1076 631 605 122 107 71 BglII A'GATC_T Cuts at: 7163 7163 Size: 7168 BlpI GC'TnA_GC Cuts at: 4585 4585 Size: 7168 Bme1580I G_kGCm'C Cuts at: 1790 2067 2129 2693 3058 5229 6385 1790 Size: 277 62 564 365 2171 1156 2573 Fragments arranged by size: 2573 2171 1156 564 365 277 62 BmgBI CAC'GTC Cuts at: 1318 2521 1318 Size: 1203 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2371 4398 4405 Size: 416 53 83 399 24 954 2027 7 Cuts at: 4405 4899 5857 442 Size: 494 958 1753 Fragments arranged by size: 2027 1753 958 954 494 416 399 83 53 24 7 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7168 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3217 3217 Size: 7168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3242 3588 3632 3682 4084 5828 1239 Size: 2003 346 44 50 402 1744 2579 Fragments arranged by size: 2579 2003 1744 402 346 50 44 Bpu10I CC'TnA_GC Cuts at: 2433 3265 2433 Size: 832 6336 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2653 4408 5293 6158 6456 2653 Size: 1755 885 865 298 3365 Fragments arranged by size: 3365 1755 885 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2651 3419 5831 882 Size: 617 1152 768 2412 2219 Fragments arranged by size: 2412 2219 1152 768 617 BsaAI yAC'GTr Cuts at: 493 1204 3171 4959 493 Size: 711 1967 1788 2702 Fragments arranged by size: 2702 1967 1788 711 BsaBI GATnn'nnATC Cuts at: 2614 3386 3691 7162 2614 Size: 772 305 3471 2620 Fragments arranged by size: 3471 2620 772 305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3197 3779 4975 5357 Size: 53 83 186 936 1664 582 1196 382 Cuts at: 5357 5992 275 Size: 635 1451 Fragments arranged by size: 1664 1451 1196 936 635 582 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5601 5999 6273 6420 6831 5601 Size: 398 274 147 411 5938 Fragments arranged by size: 5938 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2701 2731 1178 Size: 30 165 30 1298 30 5615 Fragments arranged by size: 5615 1298 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 1186 Size: 829 6339 BseYI C'CCAG_C Cuts at: 2055 2128 2268 4215 4246 4403 6371 6867 2055 Size: 73 140 1947 31 157 1968 496 2356 Fragments arranged by size: 2356 1968 1947 496 157 140 73 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2341 2553 2842 3809 3926 4022 1163 Size: 573 605 212 289 967 117 96 4309 Fragments arranged by size: 4309 967 605 573 289 212 117 96 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3111 4618 5379 6407 1540 Size: 275 186 1110 1507 761 1028 2301 Fragments arranged by size: 2301 1507 1110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3058 3668 3792 5229 5314 6385 3058 Size: 610 124 1437 85 1071 3841 Fragments arranged by size: 3841 1437 1071 610 124 85 BsmI GAATG_Cn' Cuts at: 2437 2681 3338 4026 4679 4772 2437 Size: 244 657 688 653 93 4833 Fragments arranged by size: 4833 688 657 653 244 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3188 3188 Size: 7168 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2129 2693 2876 3058 3668 3792 Size: 399 277 62 564 183 182 610 124 Cuts at: 3792 4487 5229 5314 6385 1391 Size: 695 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 695 610 564 399 277 183 182 124 85 62 BspEI T'CCGG_A Cuts at: 6831 6831 Size: 7168 BspHI T'CATG_A Cuts at: 5057 6787 5057 Size: 1730 5438 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2028 2747 3043 4265 4523 844 Size: 1184 719 296 1222 258 3489 Fragments arranged by size: 3489 1222 1184 719 296 258 BsrBI CCG'CTC Cuts at: 1394 1823 5055 1394 Size: 429 3232 3507 Fragments arranged by size: 3507 3232 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2206 2770 2892 3014 5663 5837 65 Size: 1183 958 564 122 122 2649 174 1396 Fragments arranged by size: 2649 1396 1183 958 564 174 122 122 BsrFI r'CCGG_y Cuts at: 1700 1810 5812 5999 1700 Size: 110 4002 187 2869 Fragments arranged by size: 4002 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7168 BssSI C'ACGA_G Cuts at: 5228 6240 5228 Size: 1012 6156 BstAPI GCAn_nnn'nTGC Cuts at: 794 2427 3764 794 Size: 1633 1337 4198 Fragments arranged by size: 4198 1633 1337 BstBI TT'CG_AA Cuts at: 5972 5972 Size: 7168 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7168 BstXI CCAn_nnnn'nTGG Cuts at: 2155 2276 2869 2933 3436 3775 2155 Size: 121 593 64 503 339 5548 Fragments arranged by size: 5548 593 503 339 121 64 BstYI r'GATC_y Cuts at: 1972 2221 2536 2827 3000 3592 4428 4498 4865 Size: 249 315 291 173 592 836 70 367 Cuts at: 4865 5250 5267 6719 6805 6817 7163 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 836 592 385 367 346 315 291 249 173 86 70 17 12 BtgI C'CryG_G Cuts at: 513 1065 2457 513 Size: 552 1392 5224 Fragments arranged by size: 5224 1392 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2248 3796 487 Size: 683 56 1022 1548 3859 Fragments arranged by size: 3859 1548 1022 683 56 BtsI GCAGTG_nn' Cuts at: 2629 3033 3343 4684 5478 5498 2629 Size: 404 310 1341 794 20 4299 Fragments arranged by size: 4299 1341 794 404 310 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7168 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7133 DraI TTT'AAA Cuts at: 3474 4472 3474 Size: 998 6170 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6175 809 Size: 507 4859 1802 Fragments arranged by size: 4859 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2626 3207 4354 Size: 54 1425 50 275 18 796 581 1147 Cuts at: 4354 4615 5504 7133 8 Size: 261 889 1629 43 Fragments arranged by size: 1629 1425 1147 889 796 581 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4615 1537 Size: 275 2803 4090 Fragments arranged by size: 4090 2803 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2347 2966 3186 3336 4226 5098 1353 Size: 680 314 619 220 150 890 872 3423 Fragments arranged by size: 3423 890 872 680 619 314 220 150 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5755 6129 6275 387 Size: 1064 138 4166 374 146 1280 Fragments arranged by size: 4166 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3790 3790 Size: 7168 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2926 3058 3143 3242 3551 3588 3632 Size: 393 1294 132 85 99 309 37 44 Cuts at: 3632 3682 3901 3920 4084 5231 5828 6615 6883 Size: 50 219 19 164 1147 597 787 268 Cuts at: 6883 1239 Size: 1524 Fragments arranged by size: 1524 1294 1147 787 597 393 309 268 219 164 132 99 85 50 44 37 19 EcoNI CCTnn'n_nnAGG Cuts at: 4272 4272 Size: 7168 EcoO109I rG'GnC_Cy Cuts at: 2365 4320 2365 Size: 1955 5213 EcoRI G'AATT_C Cuts at: 3538 4477 4855 3538 Size: 939 378 5851 Fragments arranged by size: 5851 939 378 EcoRV GAT'ATC Cuts at: 2401 2401 Size: 7168 FspI TGC'GCA Cuts at: 5674 5674 Size: 7168 HaeII r_GCGC'y Cuts at: 1678 3484 6315 1678 Size: 1806 2831 2531 Fragments arranged by size: 2831 2531 1806 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2324 2356 3526 3558 3572 Size: 32 135 32 752 32 1170 32 14 Cuts at: 3572 3604 3745 3777 5809 5841 5883 5915 1373 Size: 32 141 32 2032 32 42 32 2626 Fragments arranged by size: 2626 2032 1170 752 141 135 42 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2452 4512 4758 669 Size: 988 281 514 2060 246 3079 Fragments arranged by size: 3079 2060 988 514 281 246 HindIII A'AGCT_T Cuts at: 748 3717 748 Size: 2969 4199 HpaI GTT'AAC Cuts at: 4758 4758 Size: 7168 KpnI G_GTAC'C Cuts at: 4493 4493 Size: 7168 MfeI C'AATT_G Cuts at: 3402 4767 3402 Size: 1365 5803 MluI A'CGCG_T Cuts at: 7044 7044 Size: 7168 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2467 2560 3336 6282 6466 1305 Size: 1162 93 776 2946 184 2007 Fragments arranged by size: 2946 2007 1162 776 184 93 MscI TGG'CCA Cuts at: 10 64 1489 1832 2628 3209 10 Size: 54 1425 343 796 581 3969 Fragments arranged by size: 3969 1425 796 581 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 3434 3671 3773 3935 3948 5126 Size: 648 1518 750 237 102 162 13 1178 Cuts at: 5126 5485 5644 518 Size: 359 159 2042 Fragments arranged by size: 2042 1518 1178 750 648 359 237 162 159 102 13 MspA1I CmG'CkG Cuts at: 1606 2055 2325 2478 2883 2946 3744 4584 5261 Size: 449 270 153 405 63 798 840 677 Cuts at: 5261 6409 6654 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 840 798 677 449 405 270 245 153 63 NcoI C'CATG_G Cuts at: 513 1065 2457 513 Size: 552 1392 5224 Fragments arranged by size: 5224 1392 552 NdeI CA'TA_TG Cuts at: 387 3040 387 Size: 2653 4515 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7168 NotI GC'GGCC_GC Cuts at: 4615 4615 Size: 7168 NsiI A_TGCA'T Cuts at: 4026 4026 Size: 7168 NspI r_CATG'y Cuts at: 1132 1229 2561 2709 4526 6071 1132 Size: 97 1332 148 1817 1545 2229 Fragments arranged by size: 2229 1817 1545 1332 148 97 PasI CC'CwG_GG Cuts at: 1348 2869 1348 Size: 1521 5647 PciI A'CATG_T Cuts at: 6067 6067 Size: 7168 PflMI CCAn_nnn'nTGG Cuts at: 1108 2765 2794 1108 Size: 1657 29 5482 Fragments arranged by size: 5482 1657 29 PfoI T'CCnGG_A Cuts at: 2224 3219 3659 3711 2224 Size: 995 440 52 5681 Fragments arranged by size: 5681 995 440 52 PmeI GTTT'AAAC Cuts at: 4472 4472 Size: 7168 PmlI CAC'GTG Cuts at: 3171 3171 Size: 7168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5237 5269 6750 6782 4040 Size: 32 1165 32 1481 32 4426 Fragments arranged by size: 4426 1481 1165 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2365 2365 Size: 7168 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7168 PsiI TTA'TAA Cuts at: 4738 4738 Size: 7168 PstI C_TGCA'G Cuts at: 830 3743 4317 4520 830 Size: 2913 574 203 3478 Fragments arranged by size: 3478 2913 574 203 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7168 PvuII CAG'CTG Cuts at: 2055 2325 2478 2883 2946 3744 2055 Size: 270 153 405 63 798 5479 Fragments arranged by size: 5479 798 405 270 153 63 SacI G_AGCT'C Cuts at: 3792 3792 Size: 7168 SalI G'TCGA_C Cuts at: 1936 4510 1936 Size: 2574 4594 SanDI GG'GwC_CC Cuts at: 2365 2365 Size: 7168 SapI GCTCTTCn'nnn_ Cuts at: 4226 4226 Size: 7168 SbfI CC_TGCA'GG Cuts at: 4317 4520 4317 Size: 203 6965 ScaI AGT'ACT Cuts at: 5416 7128 5416 Size: 1712 5456 SexAI A'CCwGG_T Cuts at: 4187 4187 Size: 7168 SfcI C'TryA_G Cuts at: 826 1045 2107 2467 2878 3739 4313 4516 5651 Size: 219 1062 360 411 861 574 203 1135 Cuts at: 5651 6332 6523 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1062 861 681 574 411 360 219 203 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7168 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7168 SmaI CCC'GGG Cuts at: 1872 4495 1872 Size: 2623 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2632 3543 4423 5272 6173 6435 Size: 197 926 689 911 880 849 901 262 Cuts at: 6435 820 Size: 1553 Fragments arranged by size: 1553 926 911 901 880 849 689 262 197 SnaBI TAC'GTA Cuts at: 493 4959 493 Size: 4466 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7168 SphI G_CATG'C Cuts at: 1132 2561 4526 1132 Size: 1429 1965 3774 Fragments arranged by size: 3774 1965 1429 SspI AAT'ATT Cuts at: 5 52 5092 5 Size: 47 5040 2081 Fragments arranged by size: 5040 2081 47 StuI AGG'CCT Cuts at: 1517 4208 1517 Size: 2691 4477 StyI C'CwwG_G Cuts at: 513 1065 2457 2788 3186 3369 4607 513 Size: 552 1392 331 398 183 1238 3074 Fragments arranged by size: 3074 1392 1238 552 398 331 183 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4231 5195 5212 5365 5550 5995 325 Size: 1255 2651 964 17 153 185 445 1498 Fragments arranged by size: 2651 1498 1255 964 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2393 5414 7126 Size: 274 80 33 51 1443 416 3021 1712 Cuts at: 7126 96 Size: 138 Fragments arranged by size: 3021 1712 1443 416 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2701 2733 3012 3044 3077 3109 7128 7160 2701 Size: 32 279 32 33 32 4019 32 2709 Fragments arranged by size: 4019 2709 279 33 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2363 2447 1316 Size: 1047 84 6037 Fragments arranged by size: 6037 1047 84 XbaI T'CTAG_A Cuts at: 2498 4504 2498 Size: 2006 5162 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3217 3217 Size: 7168 XhoI C'TCGA_G Cuts at: 1943 3543 1943 Size: 1600 5568 XmaI C'CCGG_G Cuts at: 1870 4493 1870 Size: 2623 4545 XmnI GAAnn'nnTTC Cuts at: 3914 5297 3914 Size: 1383 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3198 4976 276 Size: 53 83 186 936 1664 1778 2468 Fragments arranged by size: 2468 1778 1664 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspOMI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI