################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII AccI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AvrII BamHI BbeI BbrPI BcgI BciVI BclI BfrI BglII BlnI BsaAI BsaHI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstXI BstZ17I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI Hin1I HincII HindII HpaI KasI KspI MfeI MluI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 145 GGTACC G^GTACC AccII 712 CGCG CG^CG AclI 899 AACGTT AA^CGTT AcsI 691 GAATTT [GA]^AATT[CT] AlwI 828 GATCC GGATC(4/5) AocI 1125 CCTGAGG CC^T[ACGT]AGG ApoI 691 GAATTT [GA]^AATT[CT] Asp718I 145 GGTACC G^GTACC AspHI 1037 GAGCAC G[AT]GC[AT]^C AspI 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1178 TGCGCA TGC^GCA BalI 113 TGGCCA TGG^CCA BbsI 1517 GTCTTC GAAGAC(2/6) BbvCI 1169 CCTCAGC CCTCAGC(-5/-2) BglI 9 GCCCCCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 1517 GTCTTC GAAGAC(2/6) BsaBI 814 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 1037 GAGCAC G[AT]GC[AT]^C BsmI 697 GAATGC GAATGC(1/-1) BsrFI 305 ACCGGC [GA]^CCGG[CT] BstUI 712 CGCG CG^CG BstYI 827 AGATCC [GA]^GATC[CT] Bsu36I 1125 CCTGAGG CC^T[ACGT]AGG Cfr10I 305 ACCGGC [GA]^CCGG[CT] DraIII 645 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 113 TGGCCA [CT]^GGCC[GA] EarI 969 GAAGAG CTCTTC(1/4) Eco81I 1125 CCTGAGG CC^T[ACGT]AGG FspI 1178 TGCGCA TGC^GCA HaeII 1100 AGCGCC [GA]GCGC^[CT] HgaI 1526 GACGC GACGC(5/10) HindIII 490 AAGCTT A^AGCTT KpnI 145 GGTACC GGTAC^C Ksp632I 969 GAAGAG CTCTTC(1/4) MamI 814 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 827 AGATCC [GA]^GATC[CT] MluNI 113 TGGCCA TGG^CCA MscI 113 TGGCCA TGG^CCA MvnI 712 CGCG CG^CG NdeI 503 CATATG CA^TATG PflFI 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 370 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1583 GAGTC GAGTC(4/5) PshAI 288 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 899 AACGTT AA^CGTT PvuII 315 CAGCTG CAG^CTG SexAI 1670 ACCTGGT A^CC[AT]GGT SmaI 470 CCCGGG CCC^GGG TaqI 1474 TCGA T^CGA TfiI 202 GATTC G^A[AT]TC Tsp45I 1416 GTCAC ^GT[GC]AC Tth111I 1618 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1474 TCGA T^CGA Van91I 370 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 149 CCATCAAAAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 827 AGATCC [GA]^GATC[CT] XmaCI 470 CCCGGG C^CCGGG XmaI 470 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccIII 731 TCCGGA T^CCGGA 1343 TCCGGA BfaI 170 CTAG C^TAG 1523 CTAG BlpI 18 GCTGAGC GC^T[ACGT]AGC 1529 GCTCAGC Bpu1102I 18 GCTGAGC GC^T[ACGT]AGC 1529 GCTCAGC BsaI 988 GGTCTC GGTCTC(1/5) 1080 GGTCTC BsaWI 731 TCCGGA [AT]^CCGG[AT] 1343 TCCGGA BseAI 731 TCCGGA T^CCGGA 1343 TCCGGA BsgI 642 CTGCAC GTGCAG(16/14) 1663 GTGCAG BspEI 731 TCCGGA T^CCGGA 1343 TCCGGA BsrBI 24 CCGCTC CCGCTC(-3/-3) 1210 GAGCGG BstEII 1415 GGTCACC G^GT[ACGT]ACC 1600 GGTTACC BstPI 1415 GGTCACC G^GT[ACGT]ACC 1600 GGTTACC CelII 18 GCTGAGC GC^T[ACGT]AGC 1529 GCTCAGC DpnII 820 GATC ^GATC 828 GATC EcoNI 450 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1069 CCTGGGGGAGG EcoO65I 1415 GGTCACC G^GT[ACGT]ACC 1600 GGTTACC HinfI 202 GATTC G^A[ACGT]TC 1583 GAGTC MaeI 170 CTAG C^TAG 1523 CTAG MaeII 900 ACGT A^CGT 1086 ACGT MboI 820 GATC ^GATC 828 GATC MroI 731 TCCGGA T^CCGGA 1343 TCCGGA MseI 926 TTAA T^TAA 965 TTAA MslI 781 CACCTCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1341 CATCCGGATG NdeII 820 GATC ^GATC 828 GATC Sau3AI 820 GATC ^GATC 828 GATC Tru9I 926 TTAA T^TAA 965 TTAA Tsp509I 692 AATT ^AATT 850 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AciI AfaI AluI AlwNI ApaI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PpuMI PstI RsaI Sau96I ScrFI SfaNI SfcI StuI TseI TspRI