Restriction map for
pFN21AE3762
(Circular) (Six-base) MAPSORT of: pFN21AE3762.seq Check: 3952 from: 1 to: 6406
pFN21AE3762 6406 bp
With 182 enzymes: SgfI *
March 28, 2012 12:11 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 2150 3727 2150
Size: 1577 4829
AclI AA'CG_TT
Cuts at: 1563 2905 4533 4906 1563
Size: 1342 1628 373 3063
Fragments arranged by size:
3063 1628 1342 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2477 2537 3045 4469 5853 6121 1632
Size: 845 60 508 1424 1384 268 1917
Fragments arranged by size:
1917 1424 1384 845 508 268 60
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 5305 6282 1203
Size: 4102 977 1327
Fragments arranged by size:
4102 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 2790 1166
Size: 1624 4782
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6406
AlwNI CAG_nnn'CTG
Cuts at: 2218 2381 2659 3676 4149 5721 2218
Size: 163 278 1017 473 1572 2903
Fragments arranged by size:
2903 1572 1017 473 278 163
ApaI G_GGCC'C
Cuts at: 2468 2726 3074 2468
Size: 258 348 5800
Fragments arranged by size:
5800 348 258
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2696 3715 3941 4093 6202 1493
Size: 1203 1019 226 152 2109 1697
Fragments arranged by size:
2109 1697 1203 1019 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 2475 3194 3615 3731 1870
Size: 73 532 719 421 116 4545
Fragments arranged by size:
4545 719 532 421 116 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2134 2167 2134
Size: 33 6373
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 2150 2450 3727 5182
Size: 325 217 627 249 114 300 1277 1455
Cuts at: 5182 618
Size: 1842
Fragments arranged by size:
1842 1455 1277 627 325 300 249 217
114
BanII G_rGCy'C
Cuts at: 1391 2374 2468 2726 3027 3074 3129 3201 3223
Size: 983 94 258 301 47 55 72 22
Cuts at: 3223 3725 1391
Size: 502 4072
Fragments arranged by size:
4072 983 502 301 258 94 72 55
47 22
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3515 928
Size: 971 1616 3819
Fragments arranged by size:
3819 1616 971
BbvCI CC'TCA_GC
Cuts at: 3175 3175
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2020 5017 136
Size: 107 122 71 631 605 348 2997 1525
Fragments arranged by size:
2997 1525 631 605 348 122 107 71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 2024 3535 3823 2024
Size: 1511 288 4607
Fragments arranged by size:
4607 1511 288
Bme1580I G_kGCm'C
Cuts at: 1790 2399 2455 2468 2726 3074 3238 3472 4467
Size: 609 56 13 258 348 164 234 995
Cuts at: 4467 5623 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 995 609 348 258 234 164
56 13
BmgBI CAC'GTC
Cuts at: 1318 3092 1318
Size: 1774 4632
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2539 2647 3588
Size: 416 53 83 399 24 1122 108 941
Cuts at: 3588 4137 5095 442
Size: 549 958 1753
Fragments arranged by size:
1753 1122 958 941 549 416 399 108
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2282 2282
Size: 6406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2277 2358 2403 3054 5066 1239
Size: 1038 81 45 651 2012 2579
Fragments arranged by size:
2579 2012 1038 651 81 45
Bpu10I CC'TnA_GC
Cuts at: 3175 3175
Size: 6406
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4531 5396 5694 4531
Size: 865 298 5243
Fragments arranged by size:
5243 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2999 3091 5069 882
Size: 617 1500 92 1978 2219
Fragments arranged by size:
2219 1978 1500 617 92
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 2823 6400 2823
Size: 3577 2829
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2736 3348 4839 5237 5511 5658 6069 2736
Size: 612 1491 398 274 147 411 3073
Fragments arranged by size:
3073 1491 612 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6181
Fragments arranged by size:
6181 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2032 2258 2663 2909 3148 3151 3173 3600
Size: 846 226 405 246 239 3 22 427
Cuts at: 3600 3705 1186
Size: 105 3887
Fragments arranged by size:
3887 846 427 405 246 239 226 105
22 3
BseYI C'CCAG_C
Cuts at: 2056 2485 2754 3163 3266 3335 5609 6105 2056
Size: 429 269 409 103 69 2274 496 2357
Fragments arranged by size:
2357 2274 496 429 409 269 103 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 3689 1163
Size: 573 896 1057 3880
Fragments arranged by size:
3880 1057 896 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3856 4617 5645 1540
Size: 275 186 1855 761 1028 2301
Fragments arranged by size:
2301 1855 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3046 4467 4552 5623 3046
Size: 1421 85 1071 3829
Fragments arranged by size:
3829 1421 1071 85
BsmI GAATG_Cn'
Cuts at: 2708 3917 4010 2708
Size: 1209 93 5104
Fragments arranged by size:
5104 1209 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2374 2399 2455 2468 2726 3027 3046
Size: 399 584 25 56 13 258 301 19
Cuts at: 3046 3074 3129 3201 3223 3238 3472 3725 4467
Size: 28 55 72 22 15 234 253 742
Cuts at: 4467 4552 5623 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 584 399 301 258 253
234 85 72 56 55 28 25 22
19 15 13
BspEI T'CCGG_A
Cuts at: 2736 3348 6069 2736
Size: 612 2721 3073
Fragments arranged by size:
3073 2721 612
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3761 844
Size: 2917 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2031 3217 4293 1394
Size: 429 208 1186 1076 3507
Fragments arranged by size:
3507 1186 1076 429 208
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2310 5050 5237 1700
Size: 110 500 2740 187 2869
Fragments arranged by size:
2869 2740 500 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6406
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 4466 5478 4466
Size: 1012 5394
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 3420 3605 1184
Size: 2236 185 3985
Fragments arranged by size:
3985 2236 185
BstYI r'GATC_y
Cuts at: 1972 2832 3736 4103 4488 4505 5957 6043 6055
Size: 860 904 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 904 860 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3131 3131
Size: 6406
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5667
Fragments arranged by size:
5667 683 56
BtsI GCAGTG_nn'
Cuts at: 2618 2636 3186 3236 3922 4716 4736 2618
Size: 18 550 50 686 794 20 4288
Fragments arranged by size:
4288 794 686 550 50 20 18
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DraIII CAC_nnn'GTG
Cuts at: 2655 2655
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3853 4742
Size: 54 1425 50 275 18 288 1735 889
Cuts at: 4742 6371 8
Size: 1629 43
Fragments arranged by size:
1735 1629 1425 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3853 1537
Size: 275 2041 4090
Fragments arranged by size:
4090 2041 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2969 4336 1353
Size: 1616 1367 3423
Fragments arranged by size:
3423 1616 1367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4993 5367 5513 387
Size: 1064 138 3404 374 146 1280
Fragments arranged by size:
3404 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2277 2358 2403 2477 2537 3045 3054
Size: 393 645 81 45 74 60 508 9
Cuts at: 3054 4469 5066 5853 6121 1239
Size: 1415 597 787 268 1524
Fragments arranged by size:
1524 1415 787 645 597 508 393 268
81 74 60 45 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2459 3078 2459
Size: 619 5787
EcoO109I rG'GnC_Cy
Cuts at: 2053 2464 2465 2722 2723 3070 3071 3390 3622
Size: 411 1 257 1 347 1 319 232
Cuts at: 3622 3634 2053
Size: 12 4825
Fragments arranged by size:
4825 411 347 319 257 232 12 1
1 1
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2830 2830
Size: 6406
FspI TGC'GCA
Cuts at: 3185 4912 3185
Size: 1727 4679
HaeII r_GCGC'y
Cuts at: 1678 3109 5553 1678
Size: 1431 2444 2531
Fragments arranged by size:
2531 2444 1431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 3684 3716 5047
Size: 32 135 32 812 32 1268 32 1331
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1331 1268 812 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3750 3996 669
Size: 988 281 1812 246 3079
Fragments arranged by size:
3079 1812 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2495 748
Size: 1747 4659
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 2154 3731 2154
Size: 1577 4829
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 4296
Fragments arranged by size:
4296 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2790 3350 4364 4723 4882 518
Size: 648 1624 560 1014 359 159 2042
Fragments arranged by size:
2042 1624 1014 648 560 359 159
MspA1I CmG'CkG
Cuts at: 1606 2322 3297 3327 3822 4499 5647 5892 1606
Size: 716 975 30 495 677 1148 245 2120
Fragments arranged by size:
2120 1148 975 716 677 495 245 30
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4915
Fragments arranged by size:
4915 939 552
NdeI CA'TA_TG
Cuts at: 387 2509 387
Size: 2122 4284
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3764 5309 1132
Size: 97 2535 1545 2229
Fragments arranged by size:
2535 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2303 2367 2585 3074 3097 1348
Size: 955 64 218 489 23 4657
Fragments arranged by size:
4657 955 489 218 64 23
PciI A'CATG_T
Cuts at: 5305 5305
Size: 6406
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2381 1108
Size: 1273 5133
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2053 3390 3622 3634 2053
Size: 1337 232 12 4825
Fragments arranged by size:
4825 1337 232 12
PshAI GACnn'nnGTC
Cuts at: 1613 2297 1613
Size: 684 5722
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PspOMI G'GGCC_C
Cuts at: 2464 2722 3070 2464
Size: 258 348 5800
Fragments arranged by size:
5800 348 258
PstI C_TGCA'G
Cuts at: 830 2613 2631 3231 3243 3758 830
Size: 1783 18 600 12 515 3478
Fragments arranged by size:
3478 1783 600 515 18 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2322 2322
Size: 6406
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SanDI GG'GwC_CC
Cuts at: 2053 3622 2053
Size: 1569 4837
SbfI CC_TGCA'GG
Cuts at: 3758 3758
Size: 6406
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SexAI A'CCwGG_T
Cuts at: 3675 3675
Size: 6406
SfcI C'TryA_G
Cuts at: 826 1045 2609 2627 2744 3227 3239 3754 4889
Size: 219 1564 18 117 483 12 515 1135
Cuts at: 4889 5570 5761 826
Size: 681 191 1471
Fragments arranged by size:
1564 1471 1135 681 515 483 219 191
117 18 12
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 2477 3733 1872
Size: 605 1256 4545
Fragments arranged by size:
4545 1256 605
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4510 5411 5673 820
Size: 197 926 2567 901 262 1553
Fragments arranged by size:
2567 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SrfI GCCC'GGGC
Cuts at: 2477 2477
Size: 6406
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 2089 2645 3245 1517
Size: 572 556 600 4678
Fragments arranged by size:
4678 600 572 556
StyI C'CwwG_G
Cuts at: 513 1065 2004 3845 513
Size: 552 939 1841 3074
Fragments arranged by size:
3074 1841 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2666 2746 4433 4450 4603 4788 5233
Size: 1255 1086 80 1687 17 153 185 445
Cuts at: 5233 325
Size: 1498
Fragments arranged by size:
1687 1498 1255 1086 445 185 153 80
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4652 6364 96
Size: 274 80 33 51 1443 2675 1712 138
Fragments arranged by size:
2675 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6366 6398 6366
Size: 32 6374
Tth111I GACn'n_nGTC
Cuts at: 1316 3626 1316
Size: 2310 4096
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2161 2161
Size: 6406
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 2475 3731 1870
Size: 605 1256 4545
Fragments arranged by size:
4545 1256 605
XmnI GAAnn'nnTTC
Cuts at: 4535 4535
Size: 6406
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI
BsmBI BstXI BstZ17I EcoICRI EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI PsrI RsrII SacI SacII SapI SfiI SfoI
SwaI