(Linear) (Six-base) MAPSORT of: KIEE3762.seq Check: 3599 from: 1 to: 1701 KIEE3762.orf With 182 enzymes: SgfI * March 22, 2012 16:43 .. Acc65I G'GTAC_C Cuts at: 0 145 1701 Size: 145 1556 AclI AA'CG_TT Cuts at: 0 900 1701 Size: 900 801 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 532 1040 1701 Size: 472 60 508 661 Fragments arranged by size: 661 508 472 60 AleI CACnn'nnGTG Cuts at: 0 785 1701 Size: 785 916 AlwNI CAG_nnn'CTG Cuts at: 0 213 376 654 1671 1701 Size: 213 163 278 1017 30 Fragments arranged by size: 1017 278 213 163 30 ApaI G_GGCC'C Cuts at: 0 463 721 1069 1701 Size: 463 258 348 632 Fragments arranged by size: 632 463 348 258 ApoI r'AATT_y Cuts at: 0 691 1701 Size: 691 1010 AvaI C'yCGr_G Cuts at: 0 470 1189 1610 1701 Size: 470 719 421 91 Fragments arranged by size: 719 470 421 91 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 129 162 1701 Size: 129 33 1539 Fragments arranged by size: 1539 129 33 BanI G'GyrC_C Cuts at: 0 31 145 445 1701 Size: 31 114 300 1256 Fragments arranged by size: 1256 300 114 31 BanII G_rGCy'C Cuts at: 0 369 463 721 1022 1069 1124 1196 1218 Size: 369 94 258 301 47 55 72 22 Cuts at: 1218 1701 Size: 483 Fragments arranged by size: 483 369 301 258 94 72 55 47 22 BbsI GAAGACnn'nnnn_ Cuts at: 0 1510 1701 Size: 1510 191 BbvCI CC'TCA_GC Cuts at: 0 1170 1701 Size: 1170 531 BglI GCCn_nnn'nGGC Cuts at: 0 15 1701 Size: 15 1686 BlpI GC'TnA_GC Cuts at: 0 19 1530 1701 Size: 19 1511 171 Fragments arranged by size: 1511 171 19 Bme1580I G_kGCm'C Cuts at: 0 394 450 463 721 1069 1233 1467 1701 Size: 394 56 13 258 348 164 234 234 Fragments arranged by size: 394 348 258 234 234 164 56 13 BmgBI CAC'GTC Cuts at: 0 1087 1701 Size: 1087 614 BmrI ACTGGGnnnn_n' Cuts at: 0 534 642 1583 1701 Size: 534 108 941 118 Fragments arranged by size: 941 534 118 108 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 277 1701 Size: 277 1424 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 353 398 1049 1701 Size: 272 81 45 651 652 Fragments arranged by size: 652 651 272 81 45 Bpu10I CC'TnA_GC Cuts at: 0 1170 1701 Size: 1170 531 BsaI GGTCTCn'nnnn_ Cuts at: 0 994 1086 1701 Size: 994 92 615 Fragments arranged by size: 994 615 92 BsaBI GATnn'nnATC Cuts at: 0 818 1701 Size: 818 883 BsaWI w'CCGG_w Cuts at: 0 731 1343 1701 Size: 731 612 358 Fragments arranged by size: 731 612 358 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 27 253 658 904 1143 1146 1168 1595 Size: 27 226 405 246 239 3 22 427 Cuts at: 1595 1700 1701 Size: 105 1 Fragments arranged by size: 427 405 246 239 226 105 27 22 3 1 BseYI C'CCAG_C Cuts at: 0 51 480 749 1158 1261 1330 1701 Size: 51 429 269 409 103 69 371 Fragments arranged by size: 429 409 371 269 103 69 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 627 1684 1701 Size: 627 1057 17 Fragments arranged by size: 1057 627 17 BsiHKAI G_wGCw'C Cuts at: 0 1041 1701 Size: 1041 660 BsmI GAATG_Cn' Cuts at: 0 703 1701 Size: 703 998 Bsp1286I G_dGCh'C Cuts at: 0 369 394 450 463 721 1022 1041 1069 Size: 369 25 56 13 258 301 19 28 Cuts at: 1069 1124 1196 1218 1233 1467 1701 Size: 55 72 22 15 234 234 Fragments arranged by size: 369 301 258 234 234 72 56 55 28 25 22 19 15 13 BspEI T'CCGG_A Cuts at: 0 731 1343 1701 Size: 731 612 358 Fragments arranged by size: 731 612 358 BsrBI CCG'CTC Cuts at: 0 26 1212 1701 Size: 26 1186 489 Fragments arranged by size: 1186 489 26 BsrFI r'CCGG_y Cuts at: 0 305 1701 Size: 305 1396 BstEII G'GTnAC_C Cuts at: 0 1415 1600 1701 Size: 1415 185 101 Fragments arranged by size: 1415 185 101 BstYI r'GATC_y Cuts at: 0 827 1701 Size: 827 874 Bsu36I CC'TnA_GG Cuts at: 0 1126 1701 Size: 1126 575 BtsI GCAGTG_nn' Cuts at: 0 613 631 1181 1231 1701 Size: 613 18 550 50 470 Fragments arranged by size: 613 550 470 50 18 DraIII CAC_nnn'GTG Cuts at: 0 650 1701 Size: 650 1051 EaeI y'GGCC_r Cuts at: 0 113 1701 Size: 113 1588 EarI CTCTTCn'nnn_ Cuts at: 0 964 1701 Size: 964 737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 272 353 398 472 532 1040 1049 1701 Size: 272 81 45 74 60 508 9 652 Fragments arranged by size: 652 508 272 81 74 60 45 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 454 1073 1701 Size: 454 619 628 Fragments arranged by size: 628 619 454 EcoO109I rG'GnC_Cy Cuts at: 0 48 459 460 717 718 1065 1066 1385 Size: 48 411 1 257 1 347 1 319 Cuts at: 1385 1617 1629 1701 Size: 232 12 72 Fragments arranged by size: 411 347 319 257 232 72 48 12 1 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 825 1701 Size: 825 876 FspI TGC'GCA Cuts at: 0 1180 1701 Size: 1180 521 HaeII r_GCGC'y Cuts at: 0 1104 1701 Size: 1104 597 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 379 411 1679 1701 Size: 379 32 1268 22 Fragments arranged by size: 1268 379 32 22 HindIII A'AGCT_T Cuts at: 0 490 1701 Size: 490 1211 KpnI G_GTAC'C Cuts at: 0 149 1701 Size: 149 1552 MscI TGG'CCA Cuts at: 0 115 1701 Size: 115 1586 MslI CAynn'nnrTG Cuts at: 0 785 1345 1701 Size: 785 560 356 Fragments arranged by size: 785 560 356 MspA1I CmG'CkG Cuts at: 0 317 1292 1322 1701 Size: 317 975 30 379 Fragments arranged by size: 975 379 317 30 NdeI CA'TA_TG Cuts at: 0 504 1701 Size: 504 1197 PasI CC'CwG_GG Cuts at: 0 298 362 580 1069 1092 1701 Size: 298 64 218 489 23 609 Fragments arranged by size: 609 489 298 218 64 23 PflMI CCAn_nnn'nTGG Cuts at: 0 376 1701 Size: 376 1325 PpuMI rG'GwC_Cy Cuts at: 0 48 1385 1617 1629 1701 Size: 48 1337 232 12 72 Fragments arranged by size: 1337 232 72 48 12 PshAI GACnn'nnGTC Cuts at: 0 292 1701 Size: 292 1409 PspOMI G'GGCC_C Cuts at: 0 459 717 1065 1701 Size: 459 258 348 636 Fragments arranged by size: 636 459 348 258 PstI C_TGCA'G Cuts at: 0 608 626 1226 1238 1701 Size: 608 18 600 12 463 Fragments arranged by size: 608 600 463 18 12 PvuII CAG'CTG Cuts at: 0 317 1701 Size: 317 1384 SanDI GG'GwC_CC Cuts at: 0 48 1617 1701 Size: 48 1569 84 Fragments arranged by size: 1569 84 48 SexAI A'CCwGG_T Cuts at: 0 1670 1701 Size: 1670 31 SfcI C'TryA_G Cuts at: 0 604 622 739 1222 1234 1701 Size: 604 18 117 483 12 467 Fragments arranged by size: 604 483 467 117 18 12 SmaI CCC'GGG Cuts at: 0 472 1701 Size: 472 1229 SrfI GCCC'GGGC Cuts at: 0 472 1701 Size: 472 1229 StuI AGG'CCT Cuts at: 0 84 640 1240 1701 Size: 84 556 600 461 Fragments arranged by size: 600 556 461 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 661 741 1701 Size: 661 80 960 Fragments arranged by size: 960 661 80 Tth111I GACn'n_nGTC Cuts at: 0 1621 1701 Size: 1621 80 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 156 1701 Size: 156 1545 XmaI C'CCGG_G Cuts at: 0 470 1701 Size: 470 1231 Enzymes that do cut: Acc65I AclI AcuI AleI AlwNI ApaI ApoI AvaI BaeI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BsrBI BsrFI BstEII BstYI Bsu36I BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I HindIII KpnI MscI MslI MspA1I NdeI PasI PflMI PpuMI PshAI PspOMI PstI PvuII SanDI SexAI SfcI SmaI SrfI StuI TaqII Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglII BmtI BpuEI BsaAI BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspAI HincII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StyI SwaI TatI TstI XbaI XhoI XmnI ZraI