(Circular) (Six-base) MAPSORT of: pFN21AE3827.seq Check: 4431 from: 1 to: 13156 pFN21AE3827 13156 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 10966 278 Size: 53 83 186 936 9430 2468 Fragments arranged by size: 9430 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 8580 10499 1937 Size: 6643 1919 4594 Fragments arranged by size: 6643 4594 1919 Acc65I G'GTAC_C Cuts at: 10477 10477 Size: 13156 AclI AA'CG_TT Cuts at: 1563 8290 11283 11656 1563 Size: 6727 2993 373 3063 Fragments arranged by size: 6727 3063 2993 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2088 2103 2280 2453 3024 3191 3326 3482 Size: 456 15 177 173 571 167 135 156 Cuts at: 3482 4556 4752 4818 4922 5649 6084 6120 6155 Size: 1074 196 66 104 727 435 36 35 Cuts at: 6155 6651 7550 8756 9293 11219 12603 12871 1632 Size: 496 899 1206 537 1926 1384 268 1917 Fragments arranged by size: 1926 1917 1384 1206 1074 899 727 571 537 496 456 435 268 196 177 173 167 156 135 104 66 36 35 15 AfeI AGC'GCT Cuts at: 9616 9616 Size: 13156 AflII C'TTAA_G Cuts at: 820 1017 4976 820 Size: 197 3959 9000 Fragments arranged by size: 9000 3959 197 AflIII A'CryG_T Cuts at: 1203 5835 7770 12055 13032 1203 Size: 4632 1935 4285 977 1327 Fragments arranged by size: 4632 4285 1935 1327 977 AgeI A'CCGG_T Cuts at: 11987 11987 Size: 13156 AhdI GACnn_n'nnGTC Cuts at: 3057 3313 8845 11885 3057 Size: 256 5532 3040 4328 Fragments arranged by size: 5532 4328 3040 256 AleI CACnn'nnGTG Cuts at: 1166 6632 1166 Size: 5466 7690 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2238 5592 6798 1184 Size: 1054 3354 1206 7542 Fragments arranged by size: 7542 3354 1206 1054 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2140 2172 4432 4464 4852 4884 5207 5239 2140 Size: 32 2260 32 388 32 323 32 10057 Fragments arranged by size: 10057 2260 388 323 32 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3437 3779 3998 4988 5053 9667 9766 9913 10899 Size: 342 219 990 65 4614 99 147 986 Cuts at: 10899 12471 3437 Size: 1572 4122 Fragments arranged by size: 4614 4122 1572 990 986 342 219 147 99 65 ApaI G_GGCC'C Cuts at: 5629 5629 Size: 13156 ApaLI G'TGCA_C Cuts at: 2964 3566 11213 12369 2964 Size: 602 7647 1156 3751 Fragments arranged by size: 7647 3751 1156 602 ApoI r'AATT_y Cuts at: 1493 2392 3757 3916 4495 7787 8430 9042 10465 Size: 899 1365 159 579 3292 643 612 1423 Cuts at: 10465 10691 10843 12952 1493 Size: 226 152 2109 1697 Fragments arranged by size: 3292 2109 1697 1423 1365 899 643 612 579 226 159 152 AseI AT'TA_AT Cuts at: 160 1032 7088 11710 160 Size: 872 6056 4622 1606 Fragments arranged by size: 6056 4622 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 13156 AvaI C'yCGr_G Cuts at: 1870 1943 9090 9709 9800 10481 1870 Size: 73 7147 619 91 681 4545 Fragments arranged by size: 7147 4545 681 619 91 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5494 5527 5494 Size: 33 13123 BamHI G'GATC_C Cuts at: 2920 6413 10104 10486 10853 2920 Size: 3493 3691 382 367 5223 Fragments arranged by size: 5223 3691 3493 382 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2427 4324 5056 6759 10477 Size: 325 217 627 640 1897 732 1703 3718 Cuts at: 10477 11932 618 Size: 1455 1842 Fragments arranged by size: 3718 1897 1842 1703 1455 732 640 627 325 217 BanII G_rGCy'C Cuts at: 1391 2581 2976 3041 3462 5313 5589 5607 5629 Size: 1190 395 65 421 1851 276 18 22 Cuts at: 5629 5659 6239 6665 6906 9091 9609 9716 9936 Size: 30 580 426 241 2185 518 107 220 Cuts at: 9936 10137 10475 1391 Size: 201 338 4072 Fragments arranged by size: 4072 2185 1851 1190 580 518 426 421 395 338 276 241 220 201 107 65 30 22 18 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2271 3648 4140 4533 7382 8306 928 Size: 971 372 1377 492 393 2849 924 5778 Fragments arranged by size: 5778 2849 1377 971 924 492 393 372 BbvCI CC'TCA_GC Cuts at: 2534 2985 5526 6558 9611 2534 Size: 451 2541 1032 3053 6079 Fragments arranged by size: 6079 3053 2541 1032 451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2654 2688 4022 4056 11347 11381 1903 Size: 34 717 34 1334 34 7291 34 3678 Fragments arranged by size: 7291 3678 1334 717 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2597 4168 8168 11048 12258 2597 Size: 1571 4000 2880 1210 3495 Fragments arranged by size: 4000 3495 2880 1571 1210 BclI T'GATC_A Cuts at: 1548 3190 7000 8151 8916 1548 Size: 1642 3810 1151 765 5788 Fragments arranged by size: 5788 3810 1642 1151 765 BfrBI ATG'CAT Cuts at: 2509 2509 Size: 13156 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3942 4709 5105 Size: 107 122 71 631 605 2270 767 396 Cuts at: 5105 9872 11767 136 Size: 4767 1895 1525 Fragments arranged by size: 4767 2270 1895 1525 767 631 605 396 122 107 71 BglII A'GATC_T Cuts at: 7019 8533 9490 10446 13151 7019 Size: 1514 957 956 2705 7024 Fragments arranged by size: 7024 2705 1514 957 956 BlpI GC'TnA_GC Cuts at: 5307 9674 10040 10573 5307 Size: 4367 366 533 7890 Fragments arranged by size: 7890 4367 533 366 Bme1580I G_kGCm'C Cuts at: 1790 2935 2968 3239 3570 3616 5629 6764 7392 Size: 1145 33 271 331 46 2013 1135 628 Cuts at: 7392 9953 11217 12373 1790 Size: 2561 1264 1156 2573 Fragments arranged by size: 2573 2561 2013 1264 1156 1145 1135 628 331 271 46 33 BmgBI CAC'GTC Cuts at: 1318 2544 3565 3571 6055 6601 1318 Size: 1226 1021 6 2484 546 7873 Fragments arranged by size: 7873 2484 1226 1021 546 6 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3370 3407 6278 Size: 416 53 83 399 24 1953 37 2871 Cuts at: 6278 6339 7907 8471 9787 10116 10887 11845 442 Size: 61 1568 564 1316 329 771 958 1753 Fragments arranged by size: 2871 1953 1753 1568 1316 958 771 564 416 399 329 83 61 53 37 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 13156 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6301 9223 10156 6301 Size: 2922 933 9301 Fragments arranged by size: 9301 2922 933 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2697 2713 2829 3408 3573 3729 3993 4620 Size: 1458 16 116 579 165 156 264 627 Cuts at: 4620 5510 5823 6989 7770 8207 9767 9920 9989 Size: 890 313 1166 781 437 1560 153 69 Cuts at: 9989 11816 1239 Size: 1827 2579 Fragments arranged by size: 2579 1827 1560 1458 1166 890 781 627 579 437 313 264 165 156 153 116 69 16 Bpu10I CC'TnA_GC Cuts at: 2534 2985 3320 4773 5526 6030 6558 9611 2534 Size: 451 335 1453 753 504 528 3053 6079 Fragments arranged by size: 6079 3053 1453 753 528 504 451 335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2861 4292 5016 8039 8569 10019 11281 12146 12444 Size: 1431 724 3023 530 1450 1262 865 298 Cuts at: 12444 2861 Size: 3573 Fragments arranged by size: 3573 3023 1450 1431 1262 865 724 530 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2165 5710 9407 11819 882 Size: 617 666 3545 3697 2412 2219 Fragments arranged by size: 3697 3545 2412 2219 666 617 BsaAI yAC'GTr Cuts at: 493 1204 8717 10947 493 Size: 711 7513 2230 2702 Fragments arranged by size: 7513 2702 2230 711 BsaBI GATnn'nnATC Cuts at: 2787 8156 8993 10103 13150 2787 Size: 5369 837 1110 3047 2793 Fragments arranged by size: 5369 3047 2793 1110 837 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2609 10963 11345 11980 Size: 53 83 186 936 1076 8354 382 635 Cuts at: 11980 275 Size: 1451 Fragments arranged by size: 8354 1451 1076 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2968 6410 6687 6744 11589 11987 12261 12408 12819 Size: 3442 277 57 4845 398 274 147 411 Cuts at: 12819 2968 Size: 3305 Fragments arranged by size: 4845 3442 3305 411 398 277 274 147 57 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2164 2194 3321 3351 4432 Size: 30 165 30 761 30 1127 30 1081 Cuts at: 4432 4462 4852 4882 5105 5135 6834 6864 1178 Size: 30 390 30 223 30 1699 30 7470 Fragments arranged by size: 7470 1699 1127 1081 761 390 223 165 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2681 3101 3104 3743 5184 5765 6008 6620 Size: 1495 420 3 639 1441 581 243 612 Cuts at: 6620 6623 6626 6855 8912 9599 9818 9899 9902 Size: 3 3 229 2057 687 219 81 3 Cuts at: 9902 9944 10145 1186 Size: 42 201 4197 Fragments arranged by size: 4197 2057 1495 1441 687 639 612 581 420 243 229 219 201 81 42 3 3 3 3 BseYI C'CCAG_C Cuts at: 2524 4097 5629 6699 7701 9876 12359 12855 2524 Size: 1573 1532 1070 1002 2175 2483 496 2825 Fragments arranged by size: 2825 2483 2175 1573 1532 1070 1002 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2633 4011 6794 9202 9914 10134 Size: 573 498 399 1378 2783 2408 712 220 Cuts at: 10134 1163 Size: 4185 Fragments arranged by size: 4185 2783 2408 1378 712 573 498 399 220 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 10606 11367 12395 1540 Size: 275 186 8605 761 1028 2301 Fragments arranged by size: 8605 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2581 2968 3020 3570 5115 5589 5607 5684 5809 Size: 387 52 550 1545 474 18 77 125 Cuts at: 5809 6665 6906 9609 9716 9936 11217 11302 12373 Size: 856 241 2703 107 220 1281 85 1071 Cuts at: 12373 2581 Size: 3364 Fragments arranged by size: 3364 2703 1545 1281 1071 856 550 474 387 241 220 125 107 85 77 52 18 BsmI GAATG_Cn' Cuts at: 2420 2677 3303 5389 8287 8746 9451 10667 10760 Size: 257 626 2086 2898 459 705 1216 93 Cuts at: 10760 2420 Size: 4816 Fragments arranged by size: 4816 2898 2086 1216 705 626 459 257 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2805 3969 8621 2805 Size: 1164 4652 7340 Fragments arranged by size: 7340 4652 1164 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2581 2935 2968 2976 3020 3041 3239 Size: 399 791 354 33 8 44 21 198 Cuts at: 3239 3462 3570 3616 5115 5313 5589 5607 5629 Size: 223 108 46 1499 198 276 18 22 Cuts at: 5629 5659 5684 5809 6239 6665 6764 6906 7392 Size: 30 25 125 430 426 99 142 486 Cuts at: 7392 9091 9609 9716 9936 9953 10137 10475 11217 Size: 1699 518 107 220 17 184 338 742 Cuts at: 11217 11302 12373 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1699 1499 1071 791 742 518 486 430 426 399 354 338 276 223 220 198 198 184 142 125 108 107 99 85 46 44 33 30 25 22 21 18 17 8 BspEI T'CCGG_A Cuts at: 6687 12819 6687 Size: 6132 7024 BspHI T'CATG_A Cuts at: 2188 2791 3462 4894 8061 8655 11045 12775 2188 Size: 603 671 1432 3167 594 2390 1730 2569 Fragments arranged by size: 3167 2569 2390 1730 1432 671 603 594 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3655 9565 9783 10511 844 Size: 2811 5910 218 728 3489 Fragments arranged by size: 5910 3489 2811 728 218 BsrBI CCG'CTC Cuts at: 1394 1823 5126 8191 9240 9859 9925 10036 10395 Size: 429 3303 3065 1049 619 66 111 359 Cuts at: 10395 11043 1394 Size: 648 3507 Fragments arranged by size: 3507 3303 3065 1049 648 619 429 359 111 66 BsrDI GCAATG_nn' Cuts at: 65 1248 2175 3852 7765 11651 11825 65 Size: 1183 927 1677 3913 3886 174 1396 Fragments arranged by size: 3913 3886 1677 1396 1183 927 174 BsrFI r'CCGG_y Cuts at: 1700 1810 11800 11987 1700 Size: 110 9990 187 2869 Fragments arranged by size: 9990 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2135 6185 7046 8073 96 Size: 2039 4050 861 1027 5179 Fragments arranged by size: 5179 4050 2039 1027 861 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 13156 BssSI C'ACGA_G Cuts at: 9837 11216 12228 9837 Size: 1379 1012 10765 Fragments arranged by size: 10765 1379 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2321 3024 3998 4967 794 Size: 1527 703 974 969 8983 Fragments arranged by size: 8983 1527 974 969 703 BstBI TT'CG_AA Cuts at: 4499 11960 4499 Size: 7461 5695 BstEII G'GTnAC_C Cuts at: 1184 4798 1184 Size: 3614 9542 BstXI CCAn_nnnn'nTGG Cuts at: 2691 3631 5599 5621 7927 2691 Size: 940 1968 22 2306 7920 Fragments arranged by size: 7920 2306 1968 940 22 BstYI r'GATC_y Cuts at: 1972 2920 4108 6413 6964 7019 8095 8533 8935 Size: 948 1188 2305 551 55 1076 438 402 Cuts at: 8935 9303 9490 10104 10446 10486 10853 11238 11255 Size: 368 187 614 342 40 367 385 17 Cuts at: 11255 12707 12793 12805 13151 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2305 1977 1452 1188 1076 948 614 551 438 402 385 368 367 346 342 187 86 55 40 17 12 Bsu36I CC'TnA_GG Cuts at: 5730 5949 6656 10128 5730 Size: 219 707 3472 8758 Fragments arranged by size: 8758 3472 707 219 BtgI C'CryG_G Cuts at: 513 1065 2442 2572 2976 5514 5958 6544 7593 Size: 552 1377 130 404 2538 444 586 1049 Cuts at: 7593 513 Size: 6076 Fragments arranged by size: 6076 2538 1377 1049 586 552 444 404 130 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 6741 487 Size: 683 56 5515 6902 Fragments arranged by size: 6902 5515 683 56 BtsI GCAGTG_nn' Cuts at: 3331 3435 4387 4557 5021 5807 6475 7492 7586 Size: 104 952 170 464 786 668 1017 94 Cuts at: 7586 8507 10672 11466 11486 3331 Size: 921 2165 794 20 5001 Fragments arranged by size: 5001 2165 1017 952 921 794 786 668 464 170 104 94 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 13156 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 8299 8334 529 Size: 35 7735 35 5351 Fragments arranged by size: 7735 5351 35 35 DraI TTT'AAA Cuts at: 2836 6495 8295 8865 10460 2836 Size: 3659 1800 570 1595 5532 Fragments arranged by size: 5532 3659 1800 1595 570 DraIII CAC_nnn'GTG Cuts at: 2448 3024 4958 6347 2448 Size: 576 1934 1389 9257 Fragments arranged by size: 9257 1934 1389 576 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3569 12163 809 Size: 507 2253 8594 1802 Fragments arranged by size: 8594 2253 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2569 2947 4517 Size: 54 1425 50 275 18 739 378 1570 Cuts at: 4517 5076 9484 9768 10603 11492 13121 8 Size: 559 4408 284 835 889 1629 43 Fragments arranged by size: 4408 1629 1570 1425 889 835 739 559 378 284 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10603 1537 Size: 275 8791 4090 Fragments arranged by size: 8791 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 3000 3141 4684 4837 4845 5311 6222 Size: 711 936 141 1543 153 8 466 911 Cuts at: 6222 6381 6894 6954 7176 7229 7292 8660 9141 Size: 159 513 60 222 53 63 1368 481 Cuts at: 9141 9239 9246 9465 10437 11086 1353 Size: 98 7 219 972 649 3423 Fragments arranged by size: 3423 1543 1368 972 936 911 711 649 513 481 466 222 219 159 153 141 98 63 60 53 8 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 8108 11743 12117 12263 387 Size: 1064 138 6519 3635 374 146 1280 Fragments arranged by size: 6519 3635 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2579 5587 5605 6663 6904 9607 9714 9934 2579 Size: 3008 18 1058 241 2703 107 220 5801 Fragments arranged by size: 5801 3008 2703 1058 241 220 107 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2088 2103 2280 2453 2697 2713 2829 Size: 393 456 15 177 173 244 16 116 Cuts at: 2829 3024 3191 3326 3408 3482 3573 3729 3993 Size: 195 167 135 82 74 91 156 264 Cuts at: 3993 4556 4620 4752 4818 4922 5510 5649 5823 Size: 563 64 132 66 104 588 139 174 Cuts at: 5823 6084 6120 6155 6651 6989 7550 7770 8207 Size: 261 36 35 496 338 561 220 437 Cuts at: 8207 8756 9293 9767 9920 9989 11219 11816 12603 Size: 549 537 474 153 69 1230 597 787 Cuts at: 12603 12871 1239 Size: 268 1524 Fragments arranged by size: 1524 1230 787 597 588 563 561 549 537 496 474 456 437 393 338 268 264 261 244 220 195 177 174 173 167 156 153 139 135 132 116 104 91 82 74 69 66 64 36 35 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 6688 8558 9524 9917 10070 6688 Size: 1870 966 393 153 9774 Fragments arranged by size: 9774 1870 966 393 153 EcoO109I rG'GnC_Cy Cuts at: 2236 2372 4625 5625 5626 5861 6026 6704 7418 Size: 136 2253 1000 1 235 165 678 714 Cuts at: 7418 9269 9763 9865 9910 10063 10241 10303 2236 Size: 1851 494 102 45 153 178 62 5089 Fragments arranged by size: 5089 2253 1851 1000 714 678 494 235 178 165 153 136 102 62 45 1 EcoRI G'AATT_C Cuts at: 2392 3757 8430 9042 10465 10843 2392 Size: 1365 4673 612 1423 378 4705 Fragments arranged by size: 4705 4673 1423 1365 612 378 EcoRV GAT'ATC Cuts at: 6406 6406 Size: 13156 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2255 4760 6317 7580 8792 8842 9125 2255 Size: 2505 1557 1263 1212 50 283 6286 Fragments arranged by size: 6286 2505 1557 1263 1212 283 50 FspI TGC'GCA Cuts at: 11662 11662 Size: 13156 HaeII r_GCGC'y Cuts at: 1678 9618 9689 9760 12303 1678 Size: 7940 71 71 2543 2531 Fragments arranged by size: 7940 2543 2531 71 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2164 2196 2728 2760 2875 Size: 32 135 32 592 32 532 32 115 Cuts at: 2875 2907 3043 3075 3299 3331 3339 3371 3833 Size: 32 136 32 224 32 8 32 462 Cuts at: 3833 3865 5312 5344 5449 5481 6197 6229 6834 Size: 32 1447 32 105 32 716 32 605 Cuts at: 6834 6866 6956 6985 6988 7017 7568 7600 7757 Size: 32 90 29 3 29 551 32 157 Cuts at: 7757 7789 7901 7933 8080 8112 8633 8665 8831 Size: 32 112 32 147 32 521 32 166 Cuts at: 8831 8863 8920 8952 9313 9345 11797 11829 11871 Size: 32 57 32 361 32 2452 32 42 Cuts at: 11871 11903 1373 Size: 32 2626 Fragments arranged by size: 2626 2452 1447 716 605 592 551 532 521 462 361 224 166 157 147 136 135 115 112 105 90 57 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 29 8 3 HincII GTy'rAC Cuts at: 669 1657 1938 2541 4007 6937 7384 7842 8850 Size: 988 281 603 1466 2930 447 458 1008 Cuts at: 8850 10344 10500 10746 669 Size: 1494 156 246 3079 Fragments arranged by size: 3079 2930 1494 1466 1008 988 603 458 447 281 246 156 HindIII A'AGCT_T Cuts at: 748 8708 748 Size: 7960 5196 HpaI GTT'AAC Cuts at: 10344 10746 10344 Size: 402 12754 KpnI G_GTAC'C Cuts at: 10481 10481 Size: 13156 MfeI C'AATT_G Cuts at: 10755 10755 Size: 13156 MluI A'CGCG_T Cuts at: 13032 13032 Size: 13156 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2733 2875 5185 5425 6244 6832 7928 9057 Size: 1428 142 2310 240 819 588 1096 1129 Cuts at: 9057 9123 9267 12270 12454 1305 Size: 66 144 3003 184 2007 Fragments arranged by size: 3003 2310 2007 1428 1129 1096 819 588 240 184 144 142 66 MscI TGG'CCA Cuts at: 10 64 1489 1832 2949 5078 9486 9770 10 Size: 54 1425 343 1117 2129 4408 284 3396 Fragments arranged by size: 4408 3396 2129 1425 1117 343 284 54 MslI CAynn'nnrTG Cuts at: 518 1166 5597 5619 6468 6632 8660 8927 11114 Size: 648 4431 22 849 164 2028 267 2187 Cuts at: 11114 11473 11632 518 Size: 359 159 2042 Fragments arranged by size: 4431 2187 2042 2028 849 648 359 267 164 159 22 MspA1I CmG'CkG Cuts at: 1606 2574 3201 3551 3776 4913 5508 5633 5801 Size: 968 627 350 225 1137 595 125 168 Cuts at: 5801 6699 7428 7927 8461 8985 10018 10048 10572 Size: 898 729 499 534 524 1033 30 524 Cuts at: 10572 11249 12397 12642 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1137 1033 968 898 729 677 627 595 534 524 524 499 350 245 225 168 125 30 NcoI C'CATG_G Cuts at: 513 1065 5514 6544 513 Size: 552 4449 1030 7125 Fragments arranged by size: 7125 4449 1030 552 NdeI CA'TA_TG Cuts at: 387 8305 387 Size: 7918 5238 NheI G'CTAG_C Cuts at: 1052 1052 Size: 13156 NotI GC'GGCC_GC Cuts at: 10603 10603 Size: 13156 NsiI A_TGCA'T Cuts at: 2511 2511 Size: 13156 NspI r_CATG'y Cuts at: 1132 1229 4005 5839 7774 10514 12059 1132 Size: 97 2776 1834 1935 2740 1545 2229 Fragments arranged by size: 2776 2740 2229 1935 1834 1545 97 PasI CC'CwG_GG Cuts at: 1348 3032 5227 1348 Size: 1684 2195 9277 Fragments arranged by size: 9277 2195 1684 PciI A'CATG_T Cuts at: 5835 7770 12055 5835 Size: 1935 4285 6936 Fragments arranged by size: 6936 4285 1935 PflMI CCAn_nnn'nTGG Cuts at: 1108 2448 2807 3483 4871 5053 6550 6920 9667 Size: 1340 359 676 1388 182 1497 370 2747 Cuts at: 9667 9766 1108 Size: 99 4498 Fragments arranged by size: 4498 2747 1497 1388 1340 676 370 359 182 99 PfoI T'CCnGG_A Cuts at: 2280 2690 2806 4870 6255 7105 9165 2280 Size: 410 116 2064 1385 850 2060 6271 Fragments arranged by size: 6271 2064 2060 1385 850 410 116 PmeI GTTT'AAAC Cuts at: 10460 10460 Size: 13156 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3444 3476 4432 4464 4661 4693 4852 4884 10202 Size: 32 956 32 197 32 159 32 5318 Cuts at: 10202 10234 11225 11257 12738 12770 3444 Size: 32 991 32 1481 32 3830 Fragments arranged by size: 5318 3830 1481 991 956 197 159 32 32 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2372 4625 5861 7418 9269 9763 9910 10063 2372 Size: 2253 1236 1557 1851 494 147 153 5465 Fragments arranged by size: 5465 2253 1851 1557 1236 494 153 147 PshAI GACnn'nnGTC Cuts at: 1613 10068 1613 Size: 8455 4701 PsiI TTA'TAA Cuts at: 10726 10726 Size: 13156 PspOMI G'GGCC_C Cuts at: 5625 5625 Size: 13156 PspXI vC'TCGA_Gb Cuts at: 9800 9800 Size: 13156 PstI C_TGCA'G Cuts at: 830 3442 4567 4771 5183 5571 9624 9639 9780 Size: 2612 1125 204 412 388 4053 15 141 Cuts at: 9780 10508 830 Size: 728 3478 Fragments arranged by size: 4053 3478 2612 1125 728 412 388 204 141 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 13156 PvuII CAG'CTG Cuts at: 3201 3551 3776 4913 5508 5633 6699 7428 7927 Size: 350 225 1137 595 125 1066 729 499 Cuts at: 7927 8985 10018 3201 Size: 1058 1033 6339 Fragments arranged by size: 6339 1137 1066 1058 1033 729 595 499 350 225 125 RsrII CG'GwC_CG Cuts at: 7641 7641 Size: 13156 SacI G_AGCT'C Cuts at: 2581 5589 5607 6665 6906 9609 9716 9936 2581 Size: 3008 18 1058 241 2703 107 220 5801 Fragments arranged by size: 5801 3008 2703 1058 241 220 107 18 SacII CC_GC'GG Cuts at: 2575 2575 Size: 13156 SalI G'TCGA_C Cuts at: 1936 10498 1936 Size: 8562 4594 SanDI GG'GwC_CC Cuts at: 4625 7418 4625 Size: 2793 10363 SapI GCTCTTCn'nnn_ Cuts at: 4845 6381 6894 7292 4845 Size: 1536 513 398 10709 Fragments arranged by size: 10709 1536 513 398 SbfI CC_TGCA'GG Cuts at: 10508 10508 Size: 13156 ScaI AGT'ACT Cuts at: 2143 4354 5908 5944 6779 11404 13116 2143 Size: 2211 1554 36 835 4625 1712 2183 Fragments arranged by size: 4625 2211 2183 1712 1554 835 36 SexAI A'CCwGG_T Cuts at: 9543 9543 Size: 13156 SfcI C'TryA_G Cuts at: 826 1045 3109 3438 4467 4563 4767 5179 5411 Size: 219 2064 329 1029 96 204 412 232 Cuts at: 5411 5567 6111 6538 7429 8577 9620 9635 9776 Size: 156 544 427 891 1148 1043 15 141 Cuts at: 9776 10504 11639 12320 12511 826 Size: 728 1135 681 191 1471 Fragments arranged by size: 2064 1471 1148 1135 1043 1029 891 728 681 544 427 412 329 232 219 204 191 156 141 96 15 SfiI GGCCn_nnn'nGGCC Cuts at: 9872 9872 Size: 13156 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 13156 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 13156 SmaI CCC'GGG Cuts at: 1872 10483 1872 Size: 8611 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2876 4271 4976 5031 8054 8548 Size: 197 926 933 1395 705 55 3023 494 Cuts at: 8548 9800 9998 11260 12161 12423 820 Size: 1252 198 1262 901 262 1553 Fragments arranged by size: 3023 1553 1395 1262 1252 933 926 901 705 494 262 198 197 55 SnaBI TAC'GTA Cuts at: 493 10947 493 Size: 10454 2702 SpeI A'CTAG_T Cuts at: 152 5237 152 Size: 5085 8071 SphI G_CATG'C Cuts at: 1132 10514 1132 Size: 9382 3774 SspI AAT'ATT Cuts at: 5 52 3541 7683 8496 11080 5 Size: 47 3489 4142 813 2584 2081 Fragments arranged by size: 4142 3489 2584 2081 813 47 StuI AGG'CCT Cuts at: 1517 3246 4542 5828 1517 Size: 1729 1296 1286 8845 Fragments arranged by size: 8845 1729 1296 1286 StyI C'CwwG_G Cuts at: 513 1065 2625 4395 4923 5100 5514 5580 6544 Size: 552 1560 1770 528 177 414 66 964 Cuts at: 6544 7626 8364 8685 10595 513 Size: 1082 738 321 1910 3074 Fragments arranged by size: 3074 1910 1770 1560 1082 964 738 552 528 414 321 177 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2814 3970 5354 5507 6358 6465 6927 Size: 1255 1234 1156 1384 153 851 107 462 Cuts at: 6927 7629 9374 11183 11200 11353 11538 11983 325 Size: 702 1745 1809 17 153 185 445 1498 Fragments arranged by size: 1809 1745 1498 1384 1255 1234 1156 851 702 462 445 185 153 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2135 2141 3512 Size: 274 80 33 51 1443 158 6 1371 Cuts at: 3512 4352 5906 5942 6185 6298 6777 7046 8073 Size: 840 1554 36 243 113 479 269 1027 Cuts at: 8073 8820 11402 13114 96 Size: 747 2582 1712 138 Fragments arranged by size: 2582 1712 1554 1443 1371 1027 840 747 479 274 269 243 158 138 113 80 51 36 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3321 3353 5105 5137 5544 5576 7113 7145 8653 Size: 32 1752 32 407 32 1537 32 1508 Cuts at: 8653 8685 13116 13148 3321 Size: 32 4431 32 3329 Fragments arranged by size: 4431 3329 1752 1537 1508 407 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3353 1316 Size: 2037 11119 XbaI T'CTAG_A Cuts at: 6823 6874 10492 6823 Size: 51 3618 9487 Fragments arranged by size: 9487 3618 51 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3383 5767 5854 6352 7411 3383 Size: 2384 87 498 1059 9128 Fragments arranged by size: 9128 2384 1059 498 87 XhoI C'TCGA_G Cuts at: 1943 9800 1943 Size: 7857 5299 XmaI C'CCGG_G Cuts at: 1870 10481 1870 Size: 8611 4545 XmnI GAAnn'nnTTC Cuts at: 2268 7448 9042 9108 11285 2268 Size: 5180 1594 66 2177 4139 Fragments arranged by size: 5180 4139 2177 1594 66 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 10964 276 Size: 53 83 186 936 9430 2468 Fragments arranged by size: 9430 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BbeI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI SfoI SrfI SwaI