Restriction map for
pFN21AE3827
(Linear) (Six-base) MAPSORT of: KIEE3827.seq Check: 4960 from: 1 to: 8451
KIEE3827.orf
With 182 enzymes: SgfI *
July 16, 2019 14:21 ..
AccI GT'mk_AC
Cuts at: 0 6575 8451
Size: 6575 1876
AclI AA'CG_TT
Cuts at: 0 6285 8451
Size: 6285 2166
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 98 275 448 1019 1186 1321 1477
Size: 83 15 177 173 571 167 135 156
Cuts at: 1477 2551 2747 2813 2917 3644 4079 4115 4150
Size: 1074 196 66 104 727 435 36 35
Cuts at: 4150 4646 5545 6751 7288 8451
Size: 496 899 1206 537 1163
Fragments arranged by size:
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435 196 177 173 167 156 135 104
83 66 36 35 15
AfeI AGC'GCT
Cuts at: 0 7611 8451
Size: 7611 840
AflII C'TTAA_G
Cuts at: 0 2971 8451
Size: 2971 5480
AflIII A'CryG_T
Cuts at: 0 3830 5765 8451
Size: 3830 1935 2686
Fragments arranged by size:
3830 2686 1935
AhdI GACnn_n'nnGTC
Cuts at: 0 1052 1308 6840 8451
Size: 1052 256 5532 1611
Fragments arranged by size:
5532 1611 1052 256
AleI CACnn'nnGTG
Cuts at: 0 4627 8451
Size: 4627 3824
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 3587 4793 8451
Size: 233 3354 1206 3658
Fragments arranged by size:
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AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 135 167 2427 2459 2847 2879 3202 3234
Size: 135 32 2260 32 388 32 323 32
Cuts at: 3234 8451
Size: 5217
Fragments arranged by size:
5217 2260 388 323 135 32 32 32
32
AlwNI CAG_nnn'CTG
Cuts at: 0 1432 1774 1993 2983 3048 7662 7761 7908
Size: 1432 342 219 990 65 4614 99 147
Cuts at: 7908 8451
Size: 543
Fragments arranged by size:
4614 1432 990 543 342 219 147 99
65
ApaI G_GGCC'C
Cuts at: 0 3624 8451
Size: 3624 4827
ApaLI G'TGCA_C
Cuts at: 0 959 1561 8451
Size: 959 602 6890
Fragments arranged by size:
6890 959 602
ApoI r'AATT_y
Cuts at: 0 387 1752 1911 2490 5782 6425 7037 8451
Size: 387 1365 159 579 3292 643 612 1414
Fragments arranged by size:
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AseI AT'TA_AT
Cuts at: 0 5083 8451
Size: 5083 3368
AvaI C'yCGr_G
Cuts at: 0 7085 7704 7795 8451
Size: 7085 619 91 656
Fragments arranged by size:
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BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3489 3522 8451
Size: 3489 33 4929
Fragments arranged by size:
4929 3489 33
BamHI G'GATC_C
Cuts at: 0 915 4408 8099 8451
Size: 915 3493 3691 352
Fragments arranged by size:
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BanI G'GyrC_C
Cuts at: 0 422 2319 3051 4754 8451
Size: 422 1897 732 1703 3697
Fragments arranged by size:
3697 1897 1703 732 422
BanII G_rGCy'C
Cuts at: 0 576 971 1036 1457 3308 3584 3602 3624
Size: 576 395 65 421 1851 276 18 22
Cuts at: 3624 3654 4234 4660 4901 7086 7604 7711 7931
Size: 30 580 426 241 2185 518 107 220
Cuts at: 7931 8132 8451
Size: 201 319
Fragments arranged by size:
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319 276 241 220 201 107 65 30
22 18
BbsI GAAGACnn'nnnn_
Cuts at: 0 266 1643 2135 2528 5377 6301 8451
Size: 266 1377 492 393 2849 924 2150
Fragments arranged by size:
2849 2150 1377 924 492 393 266
BbvCI CC'TCA_GC
Cuts at: 0 529 980 3521 4553 7606 8451
Size: 529 451 2541 1032 3053 845
Fragments arranged by size:
3053 2541 1032 845 529 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 649 683 2017 2051 8451
Size: 649 34 1334 34 6400
Fragments arranged by size:
6400 1334 649 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 592 2163 6163 8451
Size: 592 1571 4000 2288
Fragments arranged by size:
4000 2288 1571 592
BclI T'GATC_A
Cuts at: 0 1185 4995 6146 6911 8451
Size: 1185 3810 1151 765 1540
Fragments arranged by size:
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BfrBI ATG'CAT
Cuts at: 0 504 8451
Size: 504 7947
BglI GCCn_nnn'nGGC
Cuts at: 0 1937 2704 3100 7867 8451
Size: 1937 767 396 4767 584
Fragments arranged by size:
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BglII A'GATC_T
Cuts at: 0 5014 6528 7485 8441 8451
Size: 5014 1514 957 956 10
Fragments arranged by size:
5014 1514 957 956 10
BlpI GC'TnA_GC
Cuts at: 0 3302 7669 8035 8451
Size: 3302 4367 366 416
Fragments arranged by size:
4367 3302 416 366
Bme1580I G_kGCm'C
Cuts at: 0 930 963 1234 1565 1611 3624 4759 5387
Size: 930 33 271 331 46 2013 1135 628
Cuts at: 5387 7948 8451
Size: 2561 503
Fragments arranged by size:
2561 2013 1135 930 628 503 331 271
46 33
BmgBI CAC'GTC
Cuts at: 0 539 1560 1566 4050 4596 8451
Size: 539 1021 6 2484 546 3855
Fragments arranged by size:
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BmrI ACTGGGnnnn_n'
Cuts at: 0 1365 1402 4273 4334 5902 6466 7782 8111
Size: 1365 37 2871 61 1568 564 1316 329
Cuts at: 8111 8451
Size: 340
Fragments arranged by size:
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37
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4296 7218 8151 8451
Size: 4296 2922 933 300
Fragments arranged by size:
4296 2922 933 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 708 824 1403 1568 1724 1988 2615
Size: 692 16 116 579 165 156 264 627
Cuts at: 2615 3505 3818 4984 5765 6202 7762 7915 7984
Size: 890 313 1166 781 437 1560 153 69
Cuts at: 7984 8451
Size: 467
Fragments arranged by size:
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437 313 264 165 156 153 116 69
16
Bpu10I CC'TnA_GC
Cuts at: 0 529 980 1315 2768 3521 4025 4553 7606
Size: 529 451 335 1453 753 504 528 3053
Cuts at: 7606 8451
Size: 845
Fragments arranged by size:
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335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 856 2287 3011 6034 6564 8014 8451
Size: 856 1431 724 3023 530 1450 437
Fragments arranged by size:
3023 1450 1431 856 724 530 437
BsaI GGTCTCn'nnnn_
Cuts at: 0 160 3705 7402 8451
Size: 160 3545 3697 1049
Fragments arranged by size:
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BsaAI yAC'GTr
Cuts at: 0 6712 8451
Size: 6712 1739
BsaBI GATnn'nnATC
Cuts at: 0 782 6151 6988 8098 8451
Size: 782 5369 837 1110 353
Fragments arranged by size:
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BsaHI Gr'CG_yC
Cuts at: 0 604 8451
Size: 604 7847
BsaWI w'CCGG_w
Cuts at: 0 963 4405 4682 4739 8451
Size: 963 3442 277 57 3712
Fragments arranged by size:
3712 3442 963 277 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 159 189 1316 1346 2427 2457 2847 2877
Size: 159 30 1127 30 1081 30 390 30
Cuts at: 2877 3100 3130 4829 4859 8451
Size: 223 30 1699 30 3592
Fragments arranged by size:
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30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 1096 1099 1738 3179 3760 4003 4615
Size: 676 420 3 639 1441 581 243 612
Cuts at: 4615 4618 4621 4850 6907 7594 7813 7894 7897
Size: 3 3 229 2057 687 219 81 3
Cuts at: 7897 7939 8140 8451
Size: 42 201 311
Fragments arranged by size:
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311 243 229 219 201 81 42 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 519 2092 3624 4694 5696 7871 8451
Size: 519 1573 1532 1070 1002 2175 580
Fragments arranged by size:
2175 1573 1532 1070 1002 580 519
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 628 2006 4789 7197 7909 8129 8451
Size: 229 399 1378 2783 2408 712 220 322
Fragments arranged by size:
2783 2408 1378 712 399 322 229 220
BsiHKAI G_wGCw'C
Cuts at: 0 576 963 1015 1565 3110 3584 3602 3679
Size: 576 387 52 550 1545 474 18 77
Cuts at: 3679 3804 4660 4901 7604 7711 7931 8451
Size: 125 856 241 2703 107 220 520
Fragments arranged by size:
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241 220 125 107 77 52 18
BsmI GAATG_Cn'
Cuts at: 0 415 672 1298 3384 6282 6741 7446 8451
Size: 415 257 626 2086 2898 459 705 1005
Fragments arranged by size:
2898 2086 1005 705 626 459 415 257
BsmBI CGTCTCn'nnnn_
Cuts at: 0 800 1964 6616 8451
Size: 800 1164 4652 1835
Fragments arranged by size:
4652 1835 1164 800
Bsp1286I G_dGCh'C
Cuts at: 0 576 930 963 971 1015 1036 1234 1457
Size: 576 354 33 8 44 21 198 223
Cuts at: 1457 1565 1611 3110 3308 3584 3602 3624 3654
Size: 108 46 1499 198 276 18 22 30
Cuts at: 3654 3679 3804 4234 4660 4759 4901 5387 7086
Size: 25 125 430 426 99 142 486 1699
Cuts at: 7086 7604 7711 7931 7948 8132 8451
Size: 518 107 220 17 184 319
Fragments arranged by size:
1699 1499 576 518 486 430 426 354
319 276 223 220 198 198 184 142
125 108 107 99 46 44 33 30
25 22 21 18 17 8
BspEI T'CCGG_A
Cuts at: 0 4682 8451
Size: 4682 3769
BspHI T'CATG_A
Cuts at: 0 183 786 1457 2889 6056 6650 8451
Size: 183 603 671 1432 3167 594 1801
Fragments arranged by size:
3167 1801 1432 671 603 594 183
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1650 7560 7778 8451
Size: 1650 5910 218 673
Fragments arranged by size:
5910 1650 673 218
BsrBI CCG'CTC
Cuts at: 0 3121 6186 7235 7854 7920 8031 8390 8451
Size: 3121 3065 1049 619 66 111 359 61
Fragments arranged by size:
3121 3065 1049 619 359 111 66 61
BsrDI GCAATG_nn'
Cuts at: 0 170 1847 5760 8451
Size: 170 1677 3913 2691
Fragments arranged by size:
3913 2691 1677 170
BsrGI T'GTAC_A
Cuts at: 0 130 4180 5041 6068 8451
Size: 130 4050 861 1027 2383
Fragments arranged by size:
4050 2383 1027 861 130
BssSI C'ACGA_G
Cuts at: 0 7832 8451
Size: 7832 619
BstAPI GCAn_nnn'nTGC
Cuts at: 0 316 1019 1993 2962 8451
Size: 316 703 974 969 5489
Fragments arranged by size:
5489 974 969 703 316
BstBI TT'CG_AA
Cuts at: 0 2494 8451
Size: 2494 5957
BstEII G'GTnAC_C
Cuts at: 0 2793 8451
Size: 2793 5658
BstXI CCAn_nnnn'nTGG
Cuts at: 0 686 1626 3594 3616 5922 8451
Size: 686 940 1968 22 2306 2529
Fragments arranged by size:
2529 2306 1968 940 686 22
BstYI r'GATC_y
Cuts at: 0 915 2103 4408 4959 5014 6090 6528 6930
Size: 915 1188 2305 551 55 1076 438 402
Cuts at: 6930 7298 7485 8099 8441 8451
Size: 368 187 614 342 10
Fragments arranged by size:
2305 1188 1076 915 614 551 438 402
368 342 187 55 10
Bsu36I CC'TnA_GG
Cuts at: 0 3725 3944 4651 8123 8451
Size: 3725 219 707 3472 328
Fragments arranged by size:
3725 3472 707 328 219
BtgI C'CryG_G
Cuts at: 0 437 567 971 3509 3953 4539 5588 8451
Size: 437 130 404 2538 444 586 1049 2863
Fragments arranged by size:
2863 2538 1049 586 444 437 404 130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4736 8451
Size: 4736 3715
BtsI GCAGTG_nn'
Cuts at: 0 1326 1430 2382 2552 3016 3802 4470 5487
Size: 1326 104 952 170 464 786 668 1017
Cuts at: 5487 5581 6502 8451
Size: 94 921 1949
Fragments arranged by size:
1949 1326 1017 952 921 786 668 464
170 104 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 6294 6329 8451
Size: 6294 35 2122
Fragments arranged by size:
6294 2122 35
DraI TTT'AAA
Cuts at: 0 831 4490 6290 6860 8451
Size: 831 3659 1800 570 1591
Fragments arranged by size:
3659 1800 1591 831 570
DraIII CAC_nnn'GTG
Cuts at: 0 443 1019 2953 4342 8451
Size: 443 576 1934 1389 4109
Fragments arranged by size:
4109 1934 1389 576 443
DrdI GACnn_nn'nnGTC
Cuts at: 0 1564 8451
Size: 1564 6887
EaeI y'GGCC_r
Cuts at: 0 564 942 2512 3071 7479 7763 8451
Size: 564 378 1570 559 4408 284 688
Fragments arranged by size:
4408 1570 688 564 559 378 284
EarI CTCTTCn'nnn_
Cuts at: 0 59 995 1136 2679 2832 2840 3306 4217
Size: 59 936 141 1543 153 8 466 911
Cuts at: 4217 4376 4889 4949 5171 5224 5287 6655 7136
Size: 159 513 60 222 53 63 1368 481
Cuts at: 7136 7234 7241 7460 8432 8451
Size: 98 7 219 972 19
Fragments arranged by size:
1543 1368 972 936 911 513 481 466
222 219 159 153 141 98 63 60
59 53 19 8 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 6103 8451
Size: 6103 2348
EcoICRI GAG'CTC
Cuts at: 0 574 3582 3600 4658 4899 7602 7709 7929
Size: 574 3008 18 1058 241 2703 107 220
Cuts at: 7929 8451
Size: 522
Fragments arranged by size:
3008 2703 1058 574 522 241 220 107
18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 83 98 275 448 692 708 824 1019
Size: 83 15 177 173 244 16 116 195
Cuts at: 1019 1186 1321 1403 1477 1568 1724 1988 2551
Size: 167 135 82 74 91 156 264 563
Cuts at: 2551 2615 2747 2813 2917 3505 3644 3818 4079
Size: 64 132 66 104 588 139 174 261
Cuts at: 4079 4115 4150 4646 4984 5545 5765 6202 6751
Size: 36 35 496 338 561 220 437 549
Cuts at: 6751 7288 7762 7915 7984 8451
Size: 537 474 153 69 467
Fragments arranged by size:
588 563 561 549 537 496 474 467
437 338 264 261 244 220 195 177
174 173 167 156 153 139 135 132
116 104 91 83 82 74 69 66
64 36 35 16 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4683 6553 7519 7912 8065 8451
Size: 4683 1870 966 393 153 386
Fragments arranged by size:
4683 1870 966 393 386 153
EcoO109I rG'GnC_Cy
Cuts at: 0 231 367 2620 3620 3621 3856 4021 4699
Size: 231 136 2253 1000 1 235 165 678
Cuts at: 4699 5413 7264 7758 7860 7905 8058 8236 8298
Size: 714 1851 494 102 45 153 178 62
Cuts at: 8298 8451
Size: 153
Fragments arranged by size:
2253 1851 1000 714 678 494 235 231
178 165 153 153 136 102 62 45
1
EcoRI G'AATT_C
Cuts at: 0 387 1752 6425 7037 8451
Size: 387 1365 4673 612 1414
Fragments arranged by size:
4673 1414 1365 612 387
EcoRV GAT'ATC
Cuts at: 0 4401 8451
Size: 4401 4050
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 250 2755 4312 5575 6787 6837 7120 8451
Size: 250 2505 1557 1263 1212 50 283 1331
Fragments arranged by size:
2505 1557 1331 1263 1212 283 250 50
HaeII r_GCGC'y
Cuts at: 0 7613 7684 7755 8451
Size: 7613 71 71 696
Fragments arranged by size:
7613 696 71 71
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 159 191 723 755 870 902 1038 1070
Size: 159 32 532 32 115 32 136 32
Cuts at: 1070 1294 1326 1334 1366 1828 1860 3307 3339
Size: 224 32 8 32 462 32 1447 32
Cuts at: 3339 3444 3476 4192 4224 4829 4861 4951 4980
Size: 105 32 716 32 605 32 90 29
Cuts at: 4980 4983 5012 5563 5595 5752 5784 5896 5928
Size: 3 29 551 32 157 32 112 32
Cuts at: 5928 6075 6107 6628 6660 6826 6858 6915 6947
Size: 147 32 521 32 166 32 57 32
Cuts at: 6947 7308 7340 8451
Size: 361 32 1111
Fragments arranged by size:
1447 1111 716 605 551 532 521 462
361 224 166 159 157 147 136 115
112 105 90 57 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 29
29 8 3
HincII GTy'rAC
Cuts at: 0 536 2002 4932 5379 5837 6845 8339 8451
Size: 536 1466 2930 447 458 1008 1494 112
Fragments arranged by size:
2930 1494 1466 1008 536 458 447 112
HindIII A'AGCT_T
Cuts at: 0 6703 8451
Size: 6703 1748
HpaI GTT'AAC
Cuts at: 0 8339 8451
Size: 8339 112
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 728 870 3180 3420 4239 4827 5923 7052
Size: 728 142 2310 240 819 588 1096 1129
Cuts at: 7052 7118 7262 8451
Size: 66 144 1189
Fragments arranged by size:
2310 1189 1129 1096 819 728 588 240
144 142 66
MscI TGG'CCA
Cuts at: 0 944 3073 7481 7765 8451
Size: 944 2129 4408 284 686
Fragments arranged by size:
4408 2129 944 686 284
MslI CAynn'nnrTG
Cuts at: 0 3592 3614 4463 4627 6655 6922 8451
Size: 3592 22 849 164 2028 267 1529
Fragments arranged by size:
3592 2028 1529 849 267 164 22
MspA1I CmG'CkG
Cuts at: 0 569 1196 1546 1771 2908 3503 3628 3796
Size: 569 627 350 225 1137 595 125 168
Cuts at: 3796 4694 5423 5922 6456 6980 8013 8043 8451
Size: 898 729 499 534 524 1033 30 408
Fragments arranged by size:
1137 1033 898 729 627 595 569 534
524 499 408 350 225 168 125 30
NcoI C'CATG_G
Cuts at: 0 3509 4539 8451
Size: 3509 1030 3912
Fragments arranged by size:
3912 3509 1030
NdeI CA'TA_TG
Cuts at: 0 6300 8451
Size: 6300 2151
NsiI A_TGCA'T
Cuts at: 0 506 8451
Size: 506 7945
NspI r_CATG'y
Cuts at: 0 2000 3834 5769 8451
Size: 2000 1834 1935 2682
Fragments arranged by size:
2682 2000 1935 1834
PasI CC'CwG_GG
Cuts at: 0 1027 3222 8451
Size: 1027 2195 5229
Fragments arranged by size:
5229 2195 1027
PciI A'CATG_T
Cuts at: 0 3830 5765 8451
Size: 3830 1935 2686
Fragments arranged by size:
3830 2686 1935
PflMI CCAn_nnn'nTGG
Cuts at: 0 443 802 1478 2866 3048 4545 4915 7662
Size: 443 359 676 1388 182 1497 370 2747
Cuts at: 7662 7761 8451
Size: 99 690
Fragments arranged by size:
2747 1497 1388 690 676 443 370 359
182 99
PfoI T'CCnGG_A
Cuts at: 0 275 685 801 2865 4250 5100 7160 8451
Size: 275 410 116 2064 1385 850 2060 1291
Fragments arranged by size:
2064 2060 1385 1291 850 410 275 116
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1439 1471 2427 2459 2656 2688 2847 2879
Size: 1439 32 956 32 197 32 159 32
Cuts at: 2879 8197 8229 8451
Size: 5318 32 222
Fragments arranged by size:
5318 1439 956 222 197 159 32 32
32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 367 2620 3856 5413 7264 7758 7905 8058
Size: 367 2253 1236 1557 1851 494 147 153
Cuts at: 8058 8451
Size: 393
Fragments arranged by size:
2253 1851 1557 1236 494 393 367 153
147
PshAI GACnn'nnGTC
Cuts at: 0 8063 8451
Size: 8063 388
PspOMI G'GGCC_C
Cuts at: 0 3620 8451
Size: 3620 4831
PspXI vC'TCGA_Gb
Cuts at: 0 7795 8451
Size: 7795 656
PstI C_TGCA'G
Cuts at: 0 1437 2562 2766 3178 3566 7619 7634 7775
Size: 1437 1125 204 412 388 4053 15 141
Cuts at: 7775 8451
Size: 676
Fragments arranged by size:
4053 1437 1125 676 412 388 204 141
15
PvuII CAG'CTG
Cuts at: 0 1196 1546 1771 2908 3503 3628 4694 5423
Size: 1196 350 225 1137 595 125 1066 729
Cuts at: 5423 5922 6980 8013 8451
Size: 499 1058 1033 438
Fragments arranged by size:
1196 1137 1066 1058 1033 729 595 499
438 350 225 125
RsrII CG'GwC_CG
Cuts at: 0 5636 8451
Size: 5636 2815
SacI G_AGCT'C
Cuts at: 0 576 3584 3602 4660 4901 7604 7711 7931
Size: 576 3008 18 1058 241 2703 107 220
Cuts at: 7931 8451
Size: 520
Fragments arranged by size:
3008 2703 1058 576 520 241 220 107
18
SacII CC_GC'GG
Cuts at: 0 570 8451
Size: 570 7881
SanDI GG'GwC_CC
Cuts at: 0 2620 5413 8451
Size: 2620 2793 3038
Fragments arranged by size:
3038 2793 2620
SapI GCTCTTCn'nnn_
Cuts at: 0 2840 4376 4889 5287 8451
Size: 2840 1536 513 398 3164
Fragments arranged by size:
3164 2840 1536 513 398
ScaI AGT'ACT
Cuts at: 0 138 2349 3903 3939 4774 8451
Size: 138 2211 1554 36 835 3677
Fragments arranged by size:
3677 2211 1554 835 138 36
SexAI A'CCwGG_T
Cuts at: 0 7538 8451
Size: 7538 913
SfcI C'TryA_G
Cuts at: 0 1104 1433 2462 2558 2762 3174 3406 3562
Size: 1104 329 1029 96 204 412 232 156
Cuts at: 3562 4106 4533 5424 6572 7615 7630 7771 8451
Size: 544 427 891 1148 1043 15 141 680
Fragments arranged by size:
1148 1104 1043 1029 891 680 544 427
412 329 232 204 156 141 96 15
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 7867 8451
Size: 7867 584
SmlI C'TyrA_G
Cuts at: 0 871 2266 2971 3026 6049 6543 7795 7993
Size: 871 1395 705 55 3023 494 1252 198
Cuts at: 7993 8451
Size: 458
Fragments arranged by size:
3023 1395 1252 871 705 494 458 198
55
SpeI A'CTAG_T
Cuts at: 0 3232 8451
Size: 3232 5219
SspI AAT'ATT
Cuts at: 0 1536 5678 6491 8451
Size: 1536 4142 813 1960
Fragments arranged by size:
4142 1960 1536 813
StuI AGG'CCT
Cuts at: 0 1241 2537 3823 8451
Size: 1241 1296 1286 4628
Fragments arranged by size:
4628 1296 1286 1241
StyI C'CwwG_G
Cuts at: 0 620 2390 2918 3095 3509 3575 4539 5621
Size: 620 1770 528 177 414 66 964 1082
Cuts at: 5621 6359 6680 8451
Size: 738 321 1771
Fragments arranged by size:
1771 1770 1082 964 738 620 528 414
321 177 66
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 809 1965 3349 3502 4353 4460 4922 5624
Size: 809 1156 1384 153 851 107 462 702
Cuts at: 5624 7369 8451
Size: 1745 1082
Fragments arranged by size:
1745 1384 1156 1082 851 809 702 462
153 107
TatI w'GTAC_w
Cuts at: 0 130 136 1507 2347 3901 3937 4180 4293
Size: 130 6 1371 840 1554 36 243 113
Cuts at: 4293 4772 5041 6068 6815 8451
Size: 479 269 1027 747 1636
Fragments arranged by size:
1636 1554 1371 1027 840 747 479 269
243 130 113 36 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1316 1348 3100 3132 3539 3571 5108 5140
Size: 1316 32 1752 32 407 32 1537 32
Cuts at: 5140 6648 6680 8451
Size: 1508 32 1771
Fragments arranged by size:
1771 1752 1537 1508 1316 407 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1348 8451
Size: 1348 7103
XbaI T'CTAG_A
Cuts at: 0 4818 4869 8451
Size: 4818 51 3582
Fragments arranged by size:
4818 3582 51
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1378 3762 3849 4347 5406 8451
Size: 1378 2384 87 498 1059 3045
Fragments arranged by size:
3045 2384 1378 1059 498 87
XhoI C'TCGA_G
Cuts at: 0 7795 8451
Size: 7795 656
XmnI GAAnn'nnTTC
Cuts at: 0 263 5443 7037 7103 8451
Size: 263 5180 1594 66 1348
Fragments arranged by size:
5180 1594 1348 263 66
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI
BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PasI PciI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuII RsrII
SacI SacII SanDI SapI ScaI SexAI SfcI SfiI
SmlI SpeI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AscI AsiSI AvrII BbeI
BmtI BsiEI BsiWI BsrFI BssHII BstZ17I ClaI EagI
FseI FspI FspAI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PmeI PmlI
PsiI PsrI PvuI SalI SbfI SfoI SgfI SgrAI
SmaI SnaBI SphI SrfI SwaI XmaI ZraI