################################## # NO CUT ENZYMES ( 45 enzymes) # ################################## Acc65I AflII AscI Asp718I AviII BciVI BfrI BsiWI BspDI BsrDI BssHII BstBI ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV FseI FspI HincII HindII HpaI KpnI KspI MluI NotI NspV PacI PflMI PmeI PshAI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SwaI Van91I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 4196 GACGTC GACGT^C AclI 6022 AACGTT AA^CGTT AgeI 2528 ACCGGT A^CCGGT Alw44I 2571 GTGCAC G^TGCAC Aor51HI 4217 AGCGCT AGC^GCT ApaI 7311 GGGCCC GGGCC^C ApaLI 2571 GTGCAC G^TGCAC AseI 5529 ATTAAT AT^TAAT AsnI 5529 ATTAAT AT^TAAT AspI 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 1020 GGATCC G^GATCC BbeI 3248 GGCGCC GGCGC^C BsmI 1602 GAATGC GAATGC(1/-1) Bsp1407I 1664 TGTACA T^GTACA BsrGI 1664 TGTACA T^GTACA Bst1107I 183 GTATAC GTA^TAC BstAPI 2184 GCACAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 4264 GGTAACC G^GT[ACGT]ACC BstPI 4264 GGTAACC G^GT[ACGT]ACC BstZ17I 183 GTATAC GTA^TAC DraIII 6316 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 4217 AGCGCT AGC^GCT EcoO65I 4264 GGTAACC G^GT[ACGT]ACC EcoT22I 5839 ATGCAT ATGCA^T KasI 3248 GGCGCC G^GCGCC MfeI 785 CAATTG C^AATTG MunI 785 CAATTG C^AATTG NaeI 4241 GCCGGC GCC^GGC NarI 3248 GGCGCC GG^CGCC NdeI 1217 CATATG CA^TATG NgoMIV 4241 GCCGGC G^CCGGC NruI 1356 TCGCGA TCG^CGA NsiI 5839 ATGCAT ATGCA^T PflFI 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 2528 ACCGGT A^CCGGT PshBI 5529 ATTAAT AT^TAAT Psp1406I 6022 AACGTT AA^CGTT SfoI 3248 GGCGCC GGC^GCC SmaI 470 CCCGGG CCC^GGG SpeI 7807 ACTAGT A^CTAGT SphI 7547 GCATGC GCATG^C SspBI 1664 TGTACA T^GTACA SspI 1506 AATATT AAT^ATT Tth111I 534 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 470 CCCGGG C^CCGGG XmaI 470 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 5032 ACATGT A^C[GA][CT]GT 6876 ACATGT AvrII 217 CCTAGG C^CTAGG 1467 CCTAGG BbrPI 1230 CACGTG CAC^GTG 6316 CACGTG BcgI 3280 CGAGCGGAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 8058 CGAACCCAGTGC BglI 838 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 7003 GCCACCTCGGC BlnI 217 CCTAGG C^CTAGG 1467 CCTAGG BsaAI 1230 CACGTG [CT]AC^GT[GA] 6316 CACGTG BsaBI 1249 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5005 GATGATCATC BsiEI 2526 CGACCG CG[GA][CT]^CG 3135 CGACCG BspHI 4799 TCATGA T^CATGA 7403 TCATGA BspLU11I 5032 ACATGT A^CATGT 6876 ACATGT BssSI 3237 CACGAG CACGAG(-5/-1) 6849 CACGAG MamI 1249 GATGTAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 5005 GATGATCATC NheI 3010 GCTAGC G^CTAGC 3609 GCTAGC PmaCI 1230 CACGTG CAC^GTG 6316 CACGTG PmlI 1230 CACGTG CAC^GTG 6316 CACGTG RcaI 4799 TCATGA T^CATGA 7403 TCATGA SapI 1006 GAAGAGC GCTCTTC(1/4) 1908 GCTCTTC StuI 476 AGGCCT AGG^CCT 2586 AGGCCT XbaI 2274 TCTAGA T^CTAGA 5188 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 147 enzymes) # ######################################### AccI AccII AccIII AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI AocI ApoI Asp700I AspEI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BclI BcnI BfaI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI Eam1105I EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MroI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoI XhoII XmnI