(Circular) (Six-base) MAPSORT of: pFN21AE9650.seq Check: 7042 from: 1 to: 12850 pFN21AE9650 12850 bp With 182 enzymes: SgfI * March 28, 2012 12:09 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4385 5630 9647 4385 Size: 1245 4017 7588 Fragments arranged by size: 7588 4017 1245 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6205 10660 278 Size: 53 83 186 936 4669 4455 2468 Fragments arranged by size: 4669 4455 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2189 2693 6538 10193 1937 Size: 252 504 3845 3655 4594 Fragments arranged by size: 4594 3845 3655 504 252 Acc65I G'GTAC_C Cuts at: 10171 10171 Size: 12850 AclI AA'CG_TT Cuts at: 1563 8028 10977 11350 1563 Size: 6465 2949 373 3063 Fragments arranged by size: 6465 3063 2949 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2217 3315 3833 3901 3939 4784 5013 5127 Size: 585 1098 518 68 38 845 229 114 Cuts at: 5127 5862 6942 8172 8437 8732 8912 9377 9431 Size: 735 1080 1230 265 295 180 465 54 Cuts at: 9431 10913 12297 12565 1632 Size: 1482 1384 268 1917 Fragments arranged by size: 1917 1482 1384 1230 1098 1080 845 735 585 518 465 295 268 265 229 180 114 68 54 38 AfeI AGC'GCT Cuts at: 6224 6224 Size: 12850 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 12653 AflIII A'CryG_T Cuts at: 1203 7037 8881 11749 12726 1203 Size: 5834 1844 2868 977 1327 Fragments arranged by size: 5834 2868 1844 1327 977 AgeI A'CCGG_T Cuts at: 4533 11681 4533 Size: 7148 5702 AhdI GACnn_n'nnGTC Cuts at: 6206 6472 8819 11579 6206 Size: 266 2347 2760 7477 Fragments arranged by size: 7477 2760 2347 266 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 12850 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 8613 9092 9655 9926 1184 Size: 7429 479 563 271 4108 Fragments arranged by size: 7429 4108 563 479 271 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3569 3601 6270 6284 6302 6316 3569 Size: 32 2669 14 18 14 10103 Fragments arranged by size: 10103 2669 32 18 14 14 AlwNI CAG_nnn'CTG Cuts at: 2650 4616 4951 9005 9698 10016 10593 12165 2650 Size: 1966 335 4054 693 318 577 1572 3335 Fragments arranged by size: 4054 3335 1966 1572 693 577 335 318 ApaI G_GGCC'C Cuts at: 9320 9320 Size: 12850 ApaLI G'TGCA_C Cuts at: 4576 10907 12063 4576 Size: 6331 1156 5363 Fragments arranged by size: 6331 5363 1156 ApoI r'AATT_y Cuts at: 1493 2044 2974 3278 3928 3977 4275 4735 6255 Size: 551 930 304 650 49 298 460 1520 Cuts at: 6255 7865 9136 10159 10385 10537 12646 1493 Size: 1610 1271 1023 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 1520 1271 1023 930 650 551 460 304 298 226 152 49 AseI AT'TA_AT Cuts at: 160 1032 7535 11404 160 Size: 872 6503 3869 1606 Fragments arranged by size: 6503 3869 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 12850 AvaI C'yCGr_G Cuts at: 1870 1943 2475 2485 2716 4148 5630 7853 8217 Size: 73 532 10 231 1432 1482 2223 364 Cuts at: 8217 8577 10175 1870 Size: 360 1598 4545 Fragments arranged by size: 4545 2223 1598 1482 1432 532 364 360 231 73 10 AvrII C'CTAG_G Cuts at: 2222 3472 2222 Size: 1250 11600 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2262 2295 9678 9711 2262 Size: 33 7383 33 5401 Fragments arranged by size: 7383 5401 33 33 BamHI G'GATC_C Cuts at: 3025 10180 10547 3025 Size: 7155 367 5328 Fragments arranged by size: 7155 5328 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2489 4823 5253 6869 6877 Size: 325 217 627 702 2334 430 1616 8 Cuts at: 6877 10171 11626 618 Size: 3294 1455 1842 Fragments arranged by size: 3294 2334 1842 1616 1455 702 627 430 325 217 8 BanII G_rGCy'C Cuts at: 1391 2352 3018 3915 5199 7175 7374 7692 8064 Size: 961 666 897 1284 1976 199 318 372 Cuts at: 8064 8171 8224 8367 8758 9320 10041 10169 1391 Size: 107 53 143 391 562 721 128 4072 Fragments arranged by size: 4072 1976 1284 961 897 721 666 562 391 372 318 199 143 128 107 53 BbeI G_GCGC'C Cuts at: 5257 5257 Size: 12850 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3111 3697 4941 5196 5573 6036 6384 Size: 971 1212 586 1244 255 377 463 348 Cuts at: 6384 8079 8961 9720 9986 928 Size: 1695 882 759 266 3792 Fragments arranged by size: 3792 1695 1244 1212 971 882 759 586 463 377 348 266 255 BbvCI CC'TCA_GC Cuts at: 4226 6039 6672 9756 4226 Size: 1813 633 3084 7320 Fragments arranged by size: 7320 3084 1813 633 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5274 5308 10052 10086 11041 11075 1903 Size: 34 3337 34 4744 34 955 34 3678 Fragments arranged by size: 4744 3678 3337 955 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 10742 11952 10742 Size: 1210 11640 BclI T'GATC_A Cuts at: 1548 2131 4254 5478 7012 9787 1548 Size: 583 2123 1224 1534 2775 4611 Fragments arranged by size: 4611 2775 2123 1534 1224 583 BfrBI ATG'CAT Cuts at: 7846 7846 Size: 12850 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2849 9014 11461 Size: 107 122 71 631 605 1177 6165 2447 Cuts at: 11461 136 Size: 1525 Fragments arranged by size: 6165 2447 1525 1177 631 605 122 107 71 BglII A'GATC_T Cuts at: 3985 6863 7900 12845 3985 Size: 2878 1037 4945 3990 Fragments arranged by size: 4945 3990 2878 1037 BlpI GC'TnA_GC Cuts at: 7142 8235 9294 10267 7142 Size: 1093 1059 973 9725 Fragments arranged by size: 9725 1093 1059 973 Bme1580I G_kGCm'C Cuts at: 1790 2492 4580 5413 6671 8613 9320 9591 10075 Size: 702 2088 833 1258 1942 707 271 484 Cuts at: 10075 10105 10123 10911 12067 1790 Size: 30 18 788 1156 2573 Fragments arranged by size: 2573 2088 1942 1258 1156 833 788 707 702 484 271 30 18 BmgBI CAC'GTC Cuts at: 1318 3718 1318 Size: 2400 10450 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2573 3583 5534 Size: 416 53 83 399 24 1156 1010 1951 Cuts at: 5534 5744 5816 6713 8699 10062 10581 11539 442 Size: 210 72 897 1986 1363 519 958 1753 Fragments arranged by size: 1986 1951 1753 1363 1156 1010 958 897 519 416 399 210 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 5019 5618 1056 Size: 3963 599 8288 Fragments arranged by size: 8288 3963 599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6773 8611 9357 6773 Size: 1838 746 10266 Fragments arranged by size: 10266 1838 746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2295 2847 3799 3857 3956 5498 5951 6044 Size: 1056 552 952 58 99 1542 453 93 Cuts at: 6044 6674 7785 8490 8606 9974 11510 1239 Size: 630 1111 705 116 1368 1536 2579 Fragments arranged by size: 2579 1542 1536 1368 1111 1056 952 705 630 552 453 116 99 93 58 Bpu10I CC'TnA_GC Cuts at: 4226 4383 4671 6039 6672 9021 9089 9206 9548 Size: 157 288 1368 633 2349 68 117 342 Cuts at: 9548 9756 4226 Size: 208 7320 Fragments arranged by size: 7320 2349 1368 633 342 288 208 157 117 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3371 6647 7299 7690 8995 10975 11840 12138 3371 Size: 3276 652 391 1305 1980 865 298 4083 Fragments arranged by size: 4083 3276 1980 1305 865 652 391 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2304 3953 6744 7390 11513 882 Size: 617 805 1649 2791 646 4123 2219 Fragments arranged by size: 4123 2791 2219 1649 805 646 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 8323 10641 493 Size: 711 2033 5086 2318 2702 Fragments arranged by size: 5086 2702 2318 2033 711 BsaBI GATnn'nnATC Cuts at: 3258 7014 12844 3258 Size: 3756 5830 3264 Fragments arranged by size: 5830 3756 3264 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3678 5254 6202 8539 Size: 53 83 186 936 2145 1576 948 2337 Cuts at: 8539 9659 10657 11039 11674 275 Size: 1120 998 382 635 1451 Fragments arranged by size: 2337 2145 1576 1451 1120 998 948 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3588 4533 4872 5703 11283 11681 11955 12102 12513 Size: 945 339 831 5580 398 274 147 411 Cuts at: 12513 3588 Size: 3925 Fragments arranged by size: 5580 3925 945 831 411 398 339 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3569 3599 9001 9031 9986 Size: 30 165 30 2166 30 5402 30 955 Cuts at: 9986 10016 1178 Size: 30 4012 Fragments arranged by size: 5402 4012 2166 955 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2277 2280 2527 2693 2870 3197 3923 4013 Size: 1091 3 247 166 177 327 726 90 Cuts at: 4013 4283 7320 7616 7628 7640 8171 8872 9175 Size: 270 3037 296 12 12 531 701 303 Cuts at: 9175 9605 1186 Size: 430 4431 Fragments arranged by size: 4431 3037 1091 726 701 531 430 327 303 296 270 247 177 166 90 12 12 3 BseYI C'CCAG_C Cuts at: 4777 5328 5914 6704 6816 6852 9065 9425 10022 Size: 551 586 790 112 36 2213 360 597 Cuts at: 10022 10040 10109 12053 12549 4777 Size: 18 69 1944 496 5078 Fragments arranged by size: 5078 2213 1944 790 597 586 551 496 360 112 69 36 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2343 2503 2805 6718 7504 8034 9625 Size: 573 607 160 302 3913 786 530 1591 Cuts at: 9625 1163 Size: 4388 Fragments arranged by size: 4388 3913 1591 786 607 573 530 302 160 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4534 5143 10300 11061 12089 1540 Size: 275 186 2533 609 5157 761 1028 2301 Fragments arranged by size: 5157 2533 2301 1028 761 609 275 186 BsiHKAI G_wGCw'C Cuts at: 2430 3018 3215 3915 4580 5199 6663 7143 7374 Size: 588 197 700 665 619 1464 480 231 Cuts at: 7374 8064 8171 8224 8981 10911 10996 12067 2430 Size: 690 107 53 757 1930 85 1071 3213 Fragments arranged by size: 3213 1930 1464 1071 757 700 690 665 619 588 480 231 197 107 85 53 BsmI GAATG_Cn' Cuts at: 3613 10361 10454 3613 Size: 6748 93 6009 Fragments arranged by size: 6748 6009 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3669 4085 6200 8103 9666 3669 Size: 416 2115 1903 1563 6853 Fragments arranged by size: 6853 2115 1903 1563 416 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2352 2430 2492 3018 3215 3915 4580 Size: 399 562 78 62 526 197 700 665 Cuts at: 4580 5199 5413 6663 6671 7143 7175 7374 7692 Size: 619 214 1250 8 472 32 199 318 Cuts at: 7692 8064 8171 8224 8367 8613 8758 8981 9320 Size: 372 107 53 143 246 145 223 339 Cuts at: 9320 9591 10041 10075 10105 10123 10169 10911 10996 Size: 271 450 34 30 18 46 742 85 Cuts at: 10996 12067 1391 Size: 1071 2174 Fragments arranged by size: 2174 1250 1071 742 700 665 619 562 526 472 450 399 372 339 318 271 246 223 214 199 197 145 143 107 85 78 62 53 46 34 32 30 18 8 BspEI T'CCGG_A Cuts at: 3588 4872 5703 12513 3588 Size: 1284 831 6810 3925 Fragments arranged by size: 6810 3925 1284 831 BspHI T'CATG_A Cuts at: 6804 9408 10739 12469 6804 Size: 2604 1331 1730 7185 Fragments arranged by size: 7185 2604 1730 1331 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3994 4385 4631 5322 5630 6762 9647 9808 Size: 3150 391 246 691 308 1132 2885 161 Cuts at: 9808 10205 844 Size: 397 3489 Fragments arranged by size: 3489 3150 2885 1132 691 397 391 308 246 161 BsrBI CCG'CTC Cuts at: 1394 1823 3322 3580 4586 5288 7799 9210 10737 Size: 429 1499 258 1006 702 2511 1411 1527 Cuts at: 10737 1394 Size: 3507 Fragments arranged by size: 3507 2511 1527 1499 1411 1006 702 429 258 BsrDI GCAATG_nn' Cuts at: 65 1248 11345 11519 65 Size: 1183 10097 174 1396 Fragments arranged by size: 10097 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4533 5334 6246 11494 11681 1700 Size: 110 2723 801 912 5248 187 2869 Fragments arranged by size: 5248 2869 2723 912 801 187 110 BsrGI T'GTAC_A Cuts at: 96 3669 96 Size: 3573 9277 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 12850 BssSI C'ACGA_G Cuts at: 5242 8854 10910 11922 5242 Size: 3612 2056 1012 6170 Fragments arranged by size: 6170 3612 2056 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4195 794 Size: 3401 9449 BstBI TT'CG_AA Cuts at: 11654 11654 Size: 12850 BstEII G'GTnAC_C Cuts at: 1184 6269 1184 Size: 5085 7765 BstXI CCAn_nnnn'nTGG Cuts at: 5782 5829 6726 7247 7775 9599 5782 Size: 47 897 521 528 1824 9033 Fragments arranged by size: 9033 1824 897 528 521 47 BstYI r'GATC_y Cuts at: 1972 2892 3025 3259 3985 5246 5765 5817 6863 Size: 920 133 234 726 1261 519 52 1046 Cuts at: 6863 7900 8481 10180 10547 10932 10949 12401 12487 Size: 1037 581 1699 367 385 17 1452 86 Cuts at: 12487 12499 12845 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1699 1452 1261 1046 1037 920 726 581 519 385 367 346 234 133 86 52 17 12 BstZ17I GTA'TAC Cuts at: 2190 2190 Size: 12850 Bsu36I CC'TnA_GG Cuts at: 2676 7254 8368 8991 2676 Size: 4578 1114 623 6535 Fragments arranged by size: 6535 4578 1114 623 BtgI C'CryG_G Cuts at: 513 1065 3529 3622 7641 7932 8605 9627 513 Size: 552 2464 93 4019 291 673 1022 3736 Fragments arranged by size: 4019 3736 2464 1022 673 552 291 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3019 487 Size: 683 56 1793 10318 Fragments arranged by size: 10318 1793 683 56 BtsI GCAGTG_nn' Cuts at: 5677 6765 7387 7819 7947 8375 8407 9648 9699 Size: 1088 622 432 128 428 32 1241 51 Cuts at: 9699 10366 11160 11180 5677 Size: 667 794 20 7347 Fragments arranged by size: 7347 1241 1088 794 667 622 432 428 128 51 32 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 12850 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2562 2597 6332 6367 529 Size: 35 1998 35 3735 35 7012 Fragments arranged by size: 7012 3735 1998 35 35 35 DraI TTT'AAA Cuts at: 2049 7972 7992 10154 2049 Size: 5923 20 2162 4745 Fragments arranged by size: 5923 4745 2162 20 DraIII CAC_nnn'GTG Cuts at: 8326 8326 Size: 12850 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4562 6961 9618 11857 809 Size: 507 3246 2399 2657 2239 1802 Fragments arranged by size: 3246 2657 2399 2239 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4399 5359 6167 Size: 54 1425 50 275 18 2569 960 808 Cuts at: 6167 6513 7411 8276 10297 11186 12815 8 Size: 346 898 865 2021 889 1629 43 Fragments arranged by size: 2569 2021 1629 1425 960 898 889 865 808 346 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 10297 1537 Size: 275 8485 4090 Fragments arranged by size: 8485 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2133 2193 2504 3006 3294 3920 6288 7605 Size: 780 60 311 502 288 626 2368 1317 Cuts at: 7605 8148 8833 9210 9525 9959 10780 1353 Size: 543 685 377 315 434 821 3423 Fragments arranged by size: 3423 2368 1317 821 780 685 626 543 502 434 377 315 311 288 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2867 6169 8878 8890 11437 11811 Size: 1064 138 1278 3302 2709 12 2547 374 Cuts at: 11811 11957 387 Size: 146 1280 Fragments arranged by size: 3302 2709 2547 1280 1278 1064 374 146 138 12 EcoICRI GAG'CTC Cuts at: 3016 3913 5197 7372 8062 8169 8222 3016 Size: 897 1284 2175 690 107 53 7644 Fragments arranged by size: 7644 2175 1284 897 690 107 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2217 2295 2847 3315 3799 3833 3857 Size: 393 585 78 552 468 484 34 24 Cuts at: 3857 3901 3939 3956 4784 5013 5127 5498 5862 Size: 44 38 17 828 229 114 371 364 Cuts at: 5862 5951 6044 6674 6942 7785 8172 8437 8490 Size: 89 93 630 268 843 387 265 53 Cuts at: 8490 8606 8732 8912 9377 9431 9974 10913 11510 Size: 116 126 180 465 54 543 939 597 Cuts at: 11510 12297 12565 1239 Size: 787 268 1524 Fragments arranged by size: 1524 939 843 828 787 630 597 585 552 543 484 468 465 393 387 371 364 268 268 265 229 180 126 116 114 93 89 78 54 53 44 38 34 24 17 EcoNI CCTnn'n_nnAGG Cuts at: 4172 6009 8049 9771 4172 Size: 1837 2040 1722 7251 Fragments arranged by size: 7251 2040 1837 1722 EcoO109I rG'GnC_Cy Cuts at: 2182 2647 2752 4864 5991 8581 8679 9002 9317 Size: 465 105 2112 1127 2590 98 323 315 Cuts at: 9317 2182 Size: 5715 Fragments arranged by size: 5715 2590 2112 1127 465 323 315 105 98 EcoRI G'AATT_C Cuts at: 10159 10537 10159 Size: 378 12472 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5035 5146 6445 8512 5035 Size: 111 1299 2067 9373 Fragments arranged by size: 9373 2067 1299 111 FspI TGC'GCA Cuts at: 11356 11356 Size: 12850 HaeII r_GCGC'y Cuts at: 1678 5257 5278 6226 8715 11997 1678 Size: 3579 21 948 2489 3282 2531 Fragments arranged by size: 3579 3282 2531 2489 948 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2087 2119 4901 4933 5210 Size: 32 135 32 515 32 2782 32 277 Cuts at: 5210 5242 5942 5974 5978 6010 6192 6224 6458 Size: 32 700 32 4 32 182 32 234 Cuts at: 6458 6490 7567 7599 8051 8083 8805 8837 9185 Size: 32 1077 32 452 32 722 32 348 Cuts at: 9185 9217 9371 9403 11491 11523 11565 11597 1373 Size: 32 154 32 2088 32 42 32 2626 Fragments arranged by size: 2782 2626 2088 1077 722 700 515 452 348 277 234 182 154 135 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 4 HincII GTy'rAC Cuts at: 669 1657 1938 10194 10440 669 Size: 988 281 8256 246 3079 Fragments arranged by size: 8256 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 3848 4355 5128 7558 7750 748 Size: 3100 507 773 2430 192 5848 Fragments arranged by size: 5848 3100 2430 773 507 192 HpaI GTT'AAC Cuts at: 10440 10440 Size: 12850 KasI G'GCGC_C Cuts at: 5253 5253 Size: 12850 KpnI G_GTAC'C Cuts at: 10175 10175 Size: 12850 MfeI C'AATT_G Cuts at: 2790 10449 2790 Size: 7659 5191 MluI A'CGCG_T Cuts at: 12726 12726 Size: 12850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6171 6456 10158 11964 12148 1305 Size: 4866 285 3702 1806 184 2007 Fragments arranged by size: 4866 3702 2007 1806 285 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4401 6169 6515 7413 8278 Size: 54 1425 343 2569 1768 346 898 865 Cuts at: 8278 10 Size: 4582 Fragments arranged by size: 4582 2569 1768 1425 898 865 346 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 4103 4217 5396 5649 5867 5883 Size: 648 843 2094 114 1179 253 218 16 Cuts at: 5883 7596 10808 11167 11326 518 Size: 1713 3212 359 159 2042 Fragments arranged by size: 3212 2094 2042 1713 1179 843 648 359 253 218 159 114 16 MspA1I CmG'CkG Cuts at: 1606 3400 4327 4613 5747 6676 6694 6820 7417 Size: 1794 927 286 1134 929 18 126 597 Cuts at: 7417 8239 9503 10026 10044 10266 10943 12091 12336 Size: 822 1264 523 18 222 677 1148 245 Cuts at: 12336 1606 Size: 2120 Fragments arranged by size: 2120 1794 1264 1148 1134 929 927 822 677 597 523 286 245 222 126 18 18 NaeI GCC'GGC Cuts at: 6248 6248 Size: 12850 NarI GG'CG_CC Cuts at: 5254 5254 Size: 12850 NcoI C'CATG_G Cuts at: 513 1065 7641 7932 9627 513 Size: 552 6576 291 1695 3736 Fragments arranged by size: 6576 3736 1695 552 291 NdeI CA'TA_TG Cuts at: 387 3223 387 Size: 2836 10014 NgoMIV G'CCGG_C Cuts at: 6246 6246 Size: 12850 NheI G'CTAG_C Cuts at: 1052 5015 5614 1052 Size: 3963 599 8288 Fragments arranged by size: 8288 3963 599 NotI GC'GGCC_GC Cuts at: 10297 10297 Size: 12850 NruI TCG'CGA Cuts at: 3363 3363 Size: 12850 NsiI A_TGCA'T Cuts at: 7848 7848 Size: 12850 NspI r_CATG'y Cuts at: 1132 1229 3317 4198 7041 8885 9556 10208 11753 Size: 97 2088 881 2843 1844 671 652 1545 Cuts at: 11753 1132 Size: 2229 Fragments arranged by size: 2843 2229 2088 1844 1545 881 671 652 97 PasI CC'CwG_GG Cuts at: 1348 3682 8960 1348 Size: 2334 5278 5238 Fragments arranged by size: 5278 5238 2334 PciI A'CATG_T Cuts at: 7037 8881 11749 7037 Size: 1844 2868 8138 Fragments arranged by size: 8138 2868 1844 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 12850 PfoI T'CCnGG_A Cuts at: 2546 2913 3387 5928 6828 7453 9859 2546 Size: 367 474 2541 900 625 2406 5537 Fragments arranged by size: 5537 2541 2406 900 625 474 367 PmeI GTTT'AAAC Cuts at: 10154 10154 Size: 12850 PmlI CAC'GTG Cuts at: 3237 8323 3237 Size: 5086 7764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 10919 10951 12432 12464 3569 Size: 32 7318 32 1481 32 3955 Fragments arranged by size: 7318 3955 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2182 2752 4864 5991 8679 2182 Size: 570 2112 1127 2688 6353 Fragments arranged by size: 6353 2688 2112 1127 570 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 12850 PsiI TTA'TAA Cuts at: 5439 10420 5439 Size: 4981 7869 PspOMI G'GGCC_C Cuts at: 9316 9316 Size: 12850 PspXI vC'TCGA_Gb Cuts at: 8217 8217 Size: 12850 PstI C_TGCA'G Cuts at: 830 2126 2533 4020 4332 4956 5971 7382 10202 Size: 1296 407 1487 312 624 1015 1411 2820 Cuts at: 10202 830 Size: 3478 Fragments arranged by size: 3478 2820 1487 1411 1296 1015 624 407 312 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 12850 PvuII CAG'CTG Cuts at: 3400 4327 4613 5747 6676 6694 6820 7417 8239 Size: 927 286 1134 929 18 126 597 822 Cuts at: 8239 9503 10026 3400 Size: 1264 523 6224 Fragments arranged by size: 6224 1264 1134 929 927 822 597 523 286 126 18 SacI G_AGCT'C Cuts at: 3018 3915 5199 7374 8064 8171 8224 3018 Size: 897 1284 2175 690 107 53 7644 Fragments arranged by size: 7644 2175 1284 897 690 107 53 SalI G'TCGA_C Cuts at: 1936 10192 1936 Size: 8256 4594 SanDI GG'GwC_CC Cuts at: 8679 8679 Size: 12850 SapI GCTCTTCn'nnn_ Cuts at: 3006 3920 3006 Size: 914 11936 SbfI CC_TGCA'GG Cuts at: 10202 10202 Size: 12850 ScaI AGT'ACT Cuts at: 4133 4410 5691 8147 11098 12810 4133 Size: 277 1281 2456 2951 1712 4173 Fragments arranged by size: 4173 2951 2456 1712 1281 277 SexAI A'CCwGG_T Cuts at: 3198 6265 9435 3198 Size: 3067 3170 6613 Fragments arranged by size: 6613 3170 3067 SfcI C'TryA_G Cuts at: 826 1045 2122 2529 2619 2694 4016 4328 4952 Size: 219 1077 407 90 75 1322 312 624 Cuts at: 4952 5967 6379 6838 7378 7781 8207 8243 10198 Size: 1015 412 459 540 403 426 36 1955 Cuts at: 10198 11333 12014 12205 826 Size: 1135 681 191 1471 Fragments arranged by size: 1955 1471 1322 1135 1077 1015 681 624 540 459 426 412 407 403 312 219 191 90 75 36 SfoI GGC'GCC Cuts at: 5255 5255 Size: 12850 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 12850 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 12850 SmaI CCC'GGG Cuts at: 1872 2477 10177 1872 Size: 605 7700 4545 Fragments arranged by size: 7700 4545 605 SmlI C'TyrA_G Cuts at: 820 1017 1943 3350 4148 6662 7314 7705 7853 Size: 197 926 1407 798 2514 652 391 148 Cuts at: 7853 8217 8974 10954 11855 12117 820 Size: 364 757 1980 901 262 1553 Fragments arranged by size: 2514 1980 1553 1407 926 901 798 757 652 391 364 262 197 148 SnaBI TAC'GTA Cuts at: 493 10641 493 Size: 10148 2702 SpeI A'CTAG_T Cuts at: 152 9812 152 Size: 9660 3190 SphI G_CATG'C Cuts at: 1132 9556 10208 1132 Size: 8424 652 3774 Fragments arranged by size: 8424 3774 652 SspI AAT'ATT Cuts at: 5 52 3513 10774 5 Size: 47 3461 7261 2081 Fragments arranged by size: 7261 3461 2081 47 StuI AGG'CCT Cuts at: 1517 2483 4593 1517 Size: 966 2110 9774 Fragments arranged by size: 9774 2110 966 StyI C'CwwG_G Cuts at: 513 1065 2222 2403 2421 2493 3162 3472 4173 Size: 552 1157 181 18 72 669 310 701 Cuts at: 4173 5010 6274 7641 7720 7884 7932 8000 8758 Size: 837 1264 1367 79 164 48 68 758 Cuts at: 8758 9237 9627 10289 513 Size: 479 390 662 3074 Fragments arranged by size: 3074 1367 1264 1157 837 758 701 669 662 552 479 390 310 181 164 79 72 68 48 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2940 5157 6887 8618 9640 10023 10877 Size: 1255 1360 2217 1730 1731 1022 383 854 Cuts at: 10877 10894 11047 11232 11677 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2217 1731 1730 1498 1360 1255 1022 854 445 383 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3669 4131 4408 Size: 274 80 33 51 1443 1692 462 277 Cuts at: 4408 5421 5689 5712 8145 11096 12808 96 Size: 1013 268 23 2433 2951 1712 138 Fragments arranged by size: 2951 2433 1712 1692 1443 1013 462 277 274 268 138 80 51 33 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4344 4376 5910 5942 7412 7444 7939 7971 7996 Size: 32 1534 32 1470 32 495 32 25 Cuts at: 7996 8028 9001 9033 12810 12842 4344 Size: 32 973 32 3777 32 4352 Fragments arranged by size: 4352 3777 1534 1470 973 495 32 32 32 32 32 32 32 25 Tth111I GACn'n_nGTC Cuts at: 1316 2542 1316 Size: 1226 11624 XbaI T'CTAG_A Cuts at: 4279 7193 10186 4279 Size: 2914 2993 6943 Fragments arranged by size: 6943 2993 2914 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4774 5829 8807 4774 Size: 1055 2978 8817 Fragments arranged by size: 8817 2978 1055 XhoI C'TCGA_G Cuts at: 1943 4148 7853 8217 1943 Size: 2205 3705 364 6576 Fragments arranged by size: 6576 3705 2205 364 XmaI C'CCGG_G Cuts at: 1870 2475 10175 1870 Size: 605 7700 4545 Fragments arranged by size: 7700 4545 605 XmnI GAAnn'nnTTC Cuts at: 2977 3288 3513 10979 2977 Size: 311 225 7466 4848 Fragments arranged by size: 7466 4848 311 225 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6203 10658 276 Size: 53 83 186 936 4669 4455 2468 Fragments arranged by size: 4669 4455 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BsiWI EcoRV FseI FspAI PacI PsrI RsrII SacII SfiI SrfI SwaI