(Linear) (Six-base) MAPSORT of: KIEE9650.seq Check: 7677 from: 1 to: 8145 KIEE9650.orf With 182 enzymes: SgfI * March 22, 2012 18:37 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 2380 3625 7642 8145 Size: 2380 1245 4017 503 Fragments arranged by size: 4017 2380 1245 503 AatII G_ACGT'C Cuts at: 0 4200 8145 Size: 4200 3945 AccI GT'mk_AC Cuts at: 0 184 688 4533 8145 Size: 184 504 3845 3612 Fragments arranged by size: 3845 3612 504 184 AclI AA'CG_TT Cuts at: 0 6023 8145 Size: 6023 2122 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 1310 1828 1896 1934 2779 3008 3122 Size: 212 1098 518 68 38 845 229 114 Cuts at: 3122 3857 4937 6167 6432 6727 6907 7372 7426 Size: 735 1080 1230 265 295 180 465 54 Cuts at: 7426 8145 Size: 719 Fragments arranged by size: 1230 1098 1080 845 735 719 518 465 295 265 229 212 180 114 68 54 38 AfeI AGC'GCT Cuts at: 0 4219 8145 Size: 4219 3926 AflIII A'CryG_T Cuts at: 0 5032 6876 8145 Size: 5032 1844 1269 Fragments arranged by size: 5032 1844 1269 AgeI A'CCGG_T Cuts at: 0 2528 8145 Size: 2528 5617 AhdI GACnn_n'nnGTC Cuts at: 0 4201 4467 6814 8145 Size: 4201 266 2347 1331 Fragments arranged by size: 4201 2347 1331 266 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 6608 7087 7650 7921 8145 Size: 6608 479 563 271 224 Fragments arranged by size: 6608 563 479 271 224 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1564 1596 4265 4279 4297 4311 8145 Size: 1564 32 2669 14 18 14 3834 Fragments arranged by size: 3834 2669 1564 32 18 14 14 AlwNI CAG_nnn'CTG Cuts at: 0 645 2611 2946 7000 7693 8011 8145 Size: 645 1966 335 4054 693 318 134 Fragments arranged by size: 4054 1966 693 645 335 318 134 ApaI G_GGCC'C Cuts at: 0 7315 8145 Size: 7315 830 ApaLI G'TGCA_C Cuts at: 0 2571 8145 Size: 2571 5574 ApoI r'AATT_y Cuts at: 0 39 969 1273 1923 1972 2270 2730 4250 Size: 39 930 304 650 49 298 460 1520 Cuts at: 4250 5860 7131 8145 Size: 1610 1271 1014 Fragments arranged by size: 1610 1520 1271 1014 930 650 460 304 298 49 39 AseI AT'TA_AT Cuts at: 0 5530 8145 Size: 5530 2615 AvaI C'yCGr_G Cuts at: 0 470 480 711 2143 3625 5848 6212 6572 Size: 470 10 231 1432 1482 2223 364 360 Cuts at: 6572 8145 Size: 1573 Fragments arranged by size: 2223 1573 1482 1432 470 364 360 231 10 AvrII C'CTAG_G Cuts at: 0 217 1467 8145 Size: 217 1250 6678 Fragments arranged by size: 6678 1250 217 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 257 290 7673 7706 8145 Size: 257 33 7383 33 439 Fragments arranged by size: 7383 439 257 33 33 BamHI G'GATC_C Cuts at: 0 1020 8145 Size: 1020 7125 BanI G'GyrC_C Cuts at: 0 484 2818 3248 4864 4872 8145 Size: 484 2334 430 1616 8 3273 Fragments arranged by size: 3273 2334 1616 484 430 8 BanII G_rGCy'C Cuts at: 0 347 1013 1910 3194 5170 5369 5687 6059 Size: 347 666 897 1284 1976 199 318 372 Cuts at: 6059 6166 6219 6362 6753 7315 8036 8145 Size: 107 53 143 391 562 721 109 Fragments arranged by size: 1976 1284 897 721 666 562 391 372 347 318 199 143 109 107 53 BbeI G_GCGC'C Cuts at: 0 3252 8145 Size: 3252 4893 BbsI GAAGACnn'nnnn_ Cuts at: 0 1106 1692 2936 3191 3568 4031 4379 6074 Size: 1106 586 1244 255 377 463 348 1695 Cuts at: 6074 6956 7715 7981 8145 Size: 882 759 266 164 Fragments arranged by size: 1695 1244 1106 882 759 586 463 377 348 266 255 164 BbvCI CC'TCA_GC Cuts at: 0 2221 4034 4667 7751 8145 Size: 2221 1813 633 3084 394 Fragments arranged by size: 3084 2221 1813 633 394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 3269 3303 8047 8081 8145 Size: 3269 34 4744 34 64 Fragments arranged by size: 4744 3269 64 34 34 BclI T'GATC_A Cuts at: 0 126 2249 3473 5007 7782 8145 Size: 126 2123 1224 1534 2775 363 Fragments arranged by size: 2775 2123 1534 1224 363 126 BfrBI ATG'CAT Cuts at: 0 5841 8145 Size: 5841 2304 BglI GCCn_nnn'nGGC Cuts at: 0 844 7009 8145 Size: 844 6165 1136 Fragments arranged by size: 6165 1136 844 BglII A'GATC_T Cuts at: 0 1980 4858 5895 8145 Size: 1980 2878 1037 2250 Fragments arranged by size: 2878 2250 1980 1037 BlpI GC'TnA_GC Cuts at: 0 5137 6230 7289 8145 Size: 5137 1093 1059 856 Fragments arranged by size: 5137 1093 1059 856 Bme1580I G_kGCm'C Cuts at: 0 487 2575 3408 4666 6608 7315 7586 8070 Size: 487 2088 833 1258 1942 707 271 484 Cuts at: 8070 8100 8118 8145 Size: 30 18 27 Fragments arranged by size: 2088 1942 1258 833 707 487 484 271 30 27 18 BmgBI CAC'GTC Cuts at: 0 1713 8145 Size: 1713 6432 BmrI ACTGGGnnnn_n' Cuts at: 0 568 1578 3529 3739 3811 4708 6694 8057 Size: 568 1010 1951 210 72 897 1986 1363 Cuts at: 8057 8145 Size: 88 Fragments arranged by size: 1986 1951 1363 1010 897 568 210 88 72 BmtI G_CTAG'C Cuts at: 0 3014 3613 8145 Size: 3014 599 4532 Fragments arranged by size: 4532 3014 599 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 4768 6606 7352 8145 Size: 4768 1838 746 793 Fragments arranged by size: 4768 1838 793 746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 290 842 1794 1852 1951 3493 3946 4039 Size: 290 552 952 58 99 1542 453 93 Cuts at: 4039 4669 5780 6485 6601 7969 8145 Size: 630 1111 705 116 1368 176 Fragments arranged by size: 1542 1368 1111 952 705 630 552 453 290 176 116 99 93 58 Bpu10I CC'TnA_GC Cuts at: 0 2221 2378 2666 4034 4667 7016 7084 7201 Size: 2221 157 288 1368 633 2349 68 117 Cuts at: 7201 7543 7751 8145 Size: 342 208 394 Fragments arranged by size: 2349 2221 1368 633 394 342 288 208 157 117 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1366 4642 5294 5685 6990 8145 Size: 1366 3276 652 391 1305 1155 Fragments arranged by size: 3276 1366 1305 1155 652 391 BsaI GGTCTCn'nnnn_ Cuts at: 0 299 1948 4739 5385 8145 Size: 299 1649 2791 646 2760 Fragments arranged by size: 2791 2760 1649 646 299 BsaAI yAC'GTr Cuts at: 0 1232 6318 8145 Size: 1232 5086 1827 Fragments arranged by size: 5086 1827 1232 BsaBI GATnn'nnATC Cuts at: 0 1253 5009 8145 Size: 1253 3756 3136 Fragments arranged by size: 3756 3136 1253 BsaHI Gr'CG_yC Cuts at: 0 1673 3249 4197 6534 7654 8145 Size: 1673 1576 948 2337 1120 491 Fragments arranged by size: 2337 1673 1576 1120 948 491 BsaWI w'CCGG_w Cuts at: 0 1583 2528 2867 3698 8145 Size: 1583 945 339 831 4447 Fragments arranged by size: 4447 1583 945 831 339 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1564 1594 6996 7026 7981 8011 8145 Size: 1564 30 5402 30 955 30 134 Fragments arranged by size: 5402 1564 955 134 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 272 275 522 688 865 1192 1918 2008 Size: 272 3 247 166 177 327 726 90 Cuts at: 2008 2278 5315 5611 5623 5635 6166 6867 7170 Size: 270 3037 296 12 12 531 701 303 Cuts at: 7170 7600 8145 Size: 430 545 Fragments arranged by size: 3037 726 701 545 531 430 327 303 296 272 270 247 177 166 90 12 12 3 BseYI C'CCAG_C Cuts at: 0 2772 3323 3909 4699 4811 4847 7060 7420 Size: 2772 551 586 790 112 36 2213 360 Cuts at: 7420 8017 8035 8104 8145 Size: 597 18 69 41 Fragments arranged by size: 2772 2213 790 597 586 551 360 112 69 41 36 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 338 498 800 4713 5499 6029 7620 8145 Size: 338 160 302 3913 786 530 1591 525 Fragments arranged by size: 3913 1591 786 530 525 338 302 160 BsiEI CG_ry'CG Cuts at: 0 2529 3138 8145 Size: 2529 609 5007 Fragments arranged by size: 5007 2529 609 BsiHKAI G_wGCw'C Cuts at: 0 425 1013 1210 1910 2575 3194 4658 5138 Size: 425 588 197 700 665 619 1464 480 Cuts at: 5138 5369 6059 6166 6219 6976 8145 Size: 231 690 107 53 757 1169 Fragments arranged by size: 1464 1169 757 700 690 665 619 588 480 425 231 197 107 53 BsmI GAATG_Cn' Cuts at: 0 1608 8145 Size: 1608 6537 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1664 2080 4195 6098 7661 8145 Size: 1664 416 2115 1903 1563 484 Fragments arranged by size: 2115 1903 1664 1563 484 416 Bsp1286I G_dGCh'C Cuts at: 0 347 425 487 1013 1210 1910 2575 3194 Size: 347 78 62 526 197 700 665 619 Cuts at: 3194 3408 4658 4666 5138 5170 5369 5687 6059 Size: 214 1250 8 472 32 199 318 372 Cuts at: 6059 6166 6219 6362 6608 6753 6976 7315 7586 Size: 107 53 143 246 145 223 339 271 Cuts at: 7586 8036 8070 8100 8118 8145 Size: 450 34 30 18 27 Fragments arranged by size: 1250 700 665 619 526 472 450 372 347 339 318 271 246 223 214 199 197 145 143 107 78 62 53 34 32 30 27 18 8 BspEI T'CCGG_A Cuts at: 0 1583 2867 3698 8145 Size: 1583 1284 831 4447 Fragments arranged by size: 4447 1583 1284 831 BspHI T'CATG_A Cuts at: 0 4799 7403 8145 Size: 4799 2604 742 Fragments arranged by size: 4799 2604 742 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1989 2380 2626 3317 3625 4757 7642 7803 Size: 1989 391 246 691 308 1132 2885 161 Cuts at: 7803 8145 Size: 342 Fragments arranged by size: 2885 1989 1132 691 391 342 308 246 161 BsrBI CCG'CTC Cuts at: 0 1317 1575 2581 3283 5794 7205 8145 Size: 1317 258 1006 702 2511 1411 940 Fragments arranged by size: 2511 1411 1317 1006 940 702 258 BsrFI r'CCGG_y Cuts at: 0 2528 3329 4241 8145 Size: 2528 801 912 3904 Fragments arranged by size: 3904 2528 912 801 BsrGI T'GTAC_A Cuts at: 0 1664 8145 Size: 1664 6481 BssSI C'ACGA_G Cuts at: 0 3237 6849 8145 Size: 3237 3612 1296 Fragments arranged by size: 3612 3237 1296 BstAPI GCAn_nnn'nTGC Cuts at: 0 2190 8145 Size: 2190 5955 BstEII G'GTnAC_C Cuts at: 0 4264 8145 Size: 4264 3881 BstXI CCAn_nnnn'nTGG Cuts at: 0 3777 3824 4721 5242 5770 7594 8145 Size: 3777 47 897 521 528 1824 551 Fragments arranged by size: 3777 1824 897 551 528 521 47 BstYI r'GATC_y Cuts at: 0 887 1020 1254 1980 3241 3760 3812 4858 Size: 887 133 234 726 1261 519 52 1046 Cuts at: 4858 5895 6476 8145 Size: 1037 581 1669 Fragments arranged by size: 1669 1261 1046 1037 887 726 581 519 234 133 52 BstZ17I GTA'TAC Cuts at: 0 185 8145 Size: 185 7960 Bsu36I CC'TnA_GG Cuts at: 0 671 5249 6363 6986 8145 Size: 671 4578 1114 623 1159 Fragments arranged by size: 4578 1159 1114 671 623 BtgI C'CryG_G Cuts at: 0 1524 1617 5636 5927 6600 7622 8145 Size: 1524 93 4019 291 673 1022 523 Fragments arranged by size: 4019 1524 1022 673 523 291 93 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1014 8145 Size: 1014 7131 BtsI GCAGTG_nn' Cuts at: 0 3672 4760 5382 5814 5942 6370 6402 7643 Size: 3672 1088 622 432 128 428 32 1241 Cuts at: 7643 7694 8145 Size: 51 451 Fragments arranged by size: 3672 1241 1088 622 451 432 428 128 51 32 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 557 592 4327 4362 8145 Size: 557 35 3735 35 3783 Fragments arranged by size: 3783 3735 557 35 35 DraI TTT'AAA Cuts at: 0 44 5967 5987 8145 Size: 44 5923 20 2158 Fragments arranged by size: 5923 2158 44 20 DraIII CAC_nnn'GTG Cuts at: 0 6321 8145 Size: 6321 1824 DrdI GACnn_nn'nnGTC Cuts at: 0 2557 4956 7613 8145 Size: 2557 2399 2657 532 Fragments arranged by size: 2657 2557 2399 532 EaeI y'GGCC_r Cuts at: 0 2394 3354 4162 4508 5406 6271 8145 Size: 2394 960 808 346 898 865 1874 Fragments arranged by size: 2394 1874 960 898 865 808 346 EarI CTCTTCn'nnn_ Cuts at: 0 128 188 499 1001 1289 1915 4283 5600 Size: 128 60 311 502 288 626 2368 1317 Cuts at: 5600 6143 6828 7205 7520 7954 8145 Size: 543 685 377 315 434 191 Fragments arranged by size: 2368 1317 685 626 543 502 434 377 315 311 288 191 128 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 862 4164 6873 6885 8145 Size: 862 3302 2709 12 1260 Fragments arranged by size: 3302 2709 1260 862 12 EcoICRI GAG'CTC Cuts at: 0 1011 1908 3192 5367 6057 6164 6217 8145 Size: 1011 897 1284 2175 690 107 53 1928 Fragments arranged by size: 2175 1928 1284 1011 897 690 107 53 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 212 290 842 1310 1794 1828 1852 1896 Size: 212 78 552 468 484 34 24 44 Cuts at: 1896 1934 1951 2779 3008 3122 3493 3857 3946 Size: 38 17 828 229 114 371 364 89 Cuts at: 3946 4039 4669 4937 5780 6167 6432 6485 6601 Size: 93 630 268 843 387 265 53 116 Cuts at: 6601 6727 6907 7372 7426 7969 8145 Size: 126 180 465 54 543 176 Fragments arranged by size: 843 828 630 552 543 484 468 465 387 371 364 268 265 229 212 180 176 126 116 114 93 89 78 54 53 44 38 34 24 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 2167 4004 6044 7766 8145 Size: 2167 1837 2040 1722 379 Fragments arranged by size: 2167 2040 1837 1722 379 EcoO109I rG'GnC_Cy Cuts at: 0 177 642 747 2859 3986 6576 6674 6997 Size: 177 465 105 2112 1127 2590 98 323 Cuts at: 6997 7312 8145 Size: 315 833 Fragments arranged by size: 2590 2112 1127 833 465 323 315 177 105 98 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 3030 3141 4440 6507 8145 Size: 3030 111 1299 2067 1638 Fragments arranged by size: 3030 2067 1638 1299 111 HaeII r_GCGC'y Cuts at: 0 3252 3273 4221 6710 8145 Size: 3252 21 948 2489 1435 Fragments arranged by size: 3252 2489 1435 948 21 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 82 114 2896 2928 3205 3237 3937 3969 Size: 82 32 2782 32 277 32 700 32 Cuts at: 3969 3973 4005 4187 4219 4453 4485 5562 5594 Size: 4 32 182 32 234 32 1077 32 Cuts at: 5594 6046 6078 6800 6832 7180 7212 7366 7398 Size: 452 32 722 32 348 32 154 32 Cuts at: 7398 8145 Size: 747 Fragments arranged by size: 2782 1077 747 722 700 452 348 277 234 182 154 82 32 32 32 32 32 32 32 32 32 32 32 32 4 HindIII A'AGCT_T Cuts at: 0 1843 2350 3123 5553 5745 8145 Size: 1843 507 773 2430 192 2400 Fragments arranged by size: 2430 2400 1843 773 507 192 KasI G'GCGC_C Cuts at: 0 3248 8145 Size: 3248 4897 MfeI C'AATT_G Cuts at: 0 785 8145 Size: 785 7360 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 4166 4451 8145 Size: 4166 285 3694 Fragments arranged by size: 4166 3694 285 MscI TGG'CCA Cuts at: 0 2396 4164 4510 5408 6273 8145 Size: 2396 1768 346 898 865 1872 Fragments arranged by size: 2396 1872 1768 898 865 346 MslI CAynn'nnrTG Cuts at: 0 2098 2212 3391 3644 3862 3878 5591 8145 Size: 2098 114 1179 253 218 16 1713 2554 Fragments arranged by size: 2554 2098 1713 1179 253 218 114 16 MspA1I CmG'CkG Cuts at: 0 1395 2322 2608 3742 4671 4689 4815 5412 Size: 1395 927 286 1134 929 18 126 597 Cuts at: 5412 6234 7498 8021 8039 8145 Size: 822 1264 523 18 106 Fragments arranged by size: 1395 1264 1134 929 927 822 597 523 286 126 106 18 18 NaeI GCC'GGC Cuts at: 0 4243 8145 Size: 4243 3902 NarI GG'CG_CC Cuts at: 0 3249 8145 Size: 3249 4896 NcoI C'CATG_G Cuts at: 0 5636 5927 7622 8145 Size: 5636 291 1695 523 Fragments arranged by size: 5636 1695 523 291 NdeI CA'TA_TG Cuts at: 0 1218 8145 Size: 1218 6927 NgoMIV G'CCGG_C Cuts at: 0 4241 8145 Size: 4241 3904 NheI G'CTAG_C Cuts at: 0 3010 3609 8145 Size: 3010 599 4536 Fragments arranged by size: 4536 3010 599 NruI TCG'CGA Cuts at: 0 1358 8145 Size: 1358 6787 NsiI A_TGCA'T Cuts at: 0 5843 8145 Size: 5843 2302 NspI r_CATG'y Cuts at: 0 1312 2193 5036 6880 7551 8145 Size: 1312 881 2843 1844 671 594 Fragments arranged by size: 2843 1844 1312 881 671 594 PasI CC'CwG_GG Cuts at: 0 1677 6955 8145 Size: 1677 5278 1190 Fragments arranged by size: 5278 1677 1190 PciI A'CATG_T Cuts at: 0 5032 6876 8145 Size: 5032 1844 1269 Fragments arranged by size: 5032 1844 1269 PfoI T'CCnGG_A Cuts at: 0 541 908 1382 3923 4823 5448 7854 8145 Size: 541 367 474 2541 900 625 2406 291 Fragments arranged by size: 2541 2406 900 625 541 474 367 291 PmlI CAC'GTG Cuts at: 0 1232 6318 8145 Size: 1232 5086 1827 Fragments arranged by size: 5086 1827 1232 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1564 1596 8145 Size: 1564 32 6549 Fragments arranged by size: 6549 1564 32 PpuMI rG'GwC_Cy Cuts at: 0 177 747 2859 3986 6674 8145 Size: 177 570 2112 1127 2688 1471 Fragments arranged by size: 2688 2112 1471 1127 570 177 PsiI TTA'TAA Cuts at: 0 3434 8145 Size: 3434 4711 PspOMI G'GGCC_C Cuts at: 0 7311 8145 Size: 7311 834 PspXI vC'TCGA_Gb Cuts at: 0 6212 8145 Size: 6212 1933 PstI C_TGCA'G Cuts at: 0 121 528 2015 2327 2951 3966 5377 8145 Size: 121 407 1487 312 624 1015 1411 2768 Fragments arranged by size: 2768 1487 1411 1015 624 407 312 121 PvuII CAG'CTG Cuts at: 0 1395 2322 2608 3742 4671 4689 4815 5412 Size: 1395 927 286 1134 929 18 126 597 Cuts at: 5412 6234 7498 8021 8145 Size: 822 1264 523 124 Fragments arranged by size: 1395 1264 1134 929 927 822 597 523 286 126 124 18 SacI G_AGCT'C Cuts at: 0 1013 1910 3194 5369 6059 6166 6219 8145 Size: 1013 897 1284 2175 690 107 53 1926 Fragments arranged by size: 2175 1926 1284 1013 897 690 107 53 SanDI GG'GwC_CC Cuts at: 0 6674 8145 Size: 6674 1471 SapI GCTCTTCn'nnn_ Cuts at: 0 1001 1915 8145 Size: 1001 914 6230 Fragments arranged by size: 6230 1001 914 ScaI AGT'ACT Cuts at: 0 2128 2405 3686 6142 8145 Size: 2128 277 1281 2456 2003 Fragments arranged by size: 2456 2128 2003 1281 277 SexAI A'CCwGG_T Cuts at: 0 1193 4260 7430 8145 Size: 1193 3067 3170 715 Fragments arranged by size: 3170 3067 1193 715 SfcI C'TryA_G Cuts at: 0 117 524 614 689 2011 2323 2947 3962 Size: 117 407 90 75 1322 312 624 1015 Cuts at: 3962 4374 4833 5373 5776 6202 6238 8145 Size: 412 459 540 403 426 36 1907 Fragments arranged by size: 1907 1322 1015 624 540 459 426 412 407 403 312 117 90 75 36 SfoI GGC'GCC Cuts at: 0 3250 8145 Size: 3250 4895 SmaI CCC'GGG Cuts at: 0 472 8145 Size: 472 7673 SmlI C'TyrA_G Cuts at: 0 1345 2143 4657 5309 5700 5848 6212 6969 Size: 1345 798 2514 652 391 148 364 757 Cuts at: 6969 8145 Size: 1176 Fragments arranged by size: 2514 1345 1176 798 757 652 391 364 148 SpeI A'CTAG_T Cuts at: 0 7807 8145 Size: 7807 338 SphI G_CATG'C Cuts at: 0 7551 8145 Size: 7551 594 SspI AAT'ATT Cuts at: 0 1508 8145 Size: 1508 6637 StuI AGG'CCT Cuts at: 0 478 2588 8145 Size: 478 2110 5557 Fragments arranged by size: 5557 2110 478 StyI C'CwwG_G Cuts at: 0 217 398 416 488 1157 1467 2168 3005 Size: 217 181 18 72 669 310 701 837 Cuts at: 3005 4269 5636 5715 5879 5927 5995 6753 7232 Size: 1264 1367 79 164 48 68 758 479 Cuts at: 7232 7622 8145 Size: 390 523 Fragments arranged by size: 1367 1264 837 758 701 669 523 479 390 310 217 181 164 79 72 68 48 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 935 3152 4882 6613 7635 8018 8145 Size: 935 2217 1730 1731 1022 383 127 Fragments arranged by size: 2217 1731 1730 1022 935 383 127 TatI w'GTAC_w Cuts at: 0 1664 2126 2403 3416 3684 3707 6140 8145 Size: 1664 462 277 1013 268 23 2433 2005 Fragments arranged by size: 2433 2005 1664 1013 462 277 268 23 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2339 2371 3905 3937 5407 5439 5934 5966 Size: 2339 32 1534 32 1470 32 495 32 Cuts at: 5966 5991 6023 6996 7028 8145 Size: 25 32 973 32 1117 Fragments arranged by size: 2339 1534 1470 1117 973 495 32 32 32 32 32 32 25 Tth111I GACn'n_nGTC Cuts at: 0 537 8145 Size: 537 7608 XbaI T'CTAG_A Cuts at: 0 2274 5188 8145 Size: 2274 2914 2957 Fragments arranged by size: 2957 2914 2274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2769 3824 6802 8145 Size: 2769 1055 2978 1343 Fragments arranged by size: 2978 2769 1343 1055 XhoI C'TCGA_G Cuts at: 0 2143 5848 6212 8145 Size: 2143 3705 364 1933 Fragments arranged by size: 3705 2143 1933 364 XmaI C'CCGG_G Cuts at: 0 470 8145 Size: 470 7675 XmnI GAAnn'nnTTC Cuts at: 0 972 1283 1508 8145 Size: 972 311 225 6637 Fragments arranged by size: 6637 972 311 225 ZraI GAC'GTC Cuts at: 0 4198 8145 Size: 4198 3947 Enzymes that do cut: AarI AatII AccI AclI AcuI AfeI AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NruI NsiI NspI PasI PciI PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SfoI SmaI SmlI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AflII AleI AscI AsiSI BciVI BsiWI BsrDI BssHII BstBI ClaI EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MluI NotI PacI PflMI PmeI PshAI PsrI PvuI RsrII SacII SalI SbfI SfiI SgfI SgrAI SnaBI SrfI SwaI