################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI Aor51HI AscI Asp718I AvaI AviII BbeI BbrPI BciVI BcnI BglI BsaAI BsaHI BsiEI BsiWI BsmBI BsoBI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CpoI EagI EclXI Eco47III Eco52I EcoO65I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccIII 1513 TCCGGA T^CCGGA Alw44I 6697 GTGCAC G^TGCAC AocI 3682 CCTAAGG CC^T[ACGT]AGG ApaI 3153 GGGCCC GGGCC^C ApaLI 6697 GTGCAC G^TGCAC AseI 6778 ATTAAT AT^TAAT AsnI 6778 ATTAAT AT^TAAT AspHI 6697 GTGCAC G[AT]GC[AT]^C AvrII 3024 CCTAGG C^CTAGG BalI 2451 TGGCCA TGG^CCA BanII 3153 GGGCCC G[GA]GC[CT]^C BcgI 304 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 3024 CCTAGG C^CTAGG BlpI 2275 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2275 GCTGAGC GC^T[ACGT]AGC BseAI 1513 TCCGGA T^CCGGA BsiHKAI 6697 GTGCAC G[AT]GC[AT]^C BspDI 6142 ATCGAT AT^CGAT BspEI 1513 TCCGGA T^CCGGA BspHI 2214 TCATGA T^CATGA BsrFI 5139 GCCGGT [GA]^CCGG[CT] Bsu36I 3682 CCTAAGG CC^T[ACGT]AGG CelII 2275 GCTGAGC GC^T[ACGT]AGC CfoI 3886 GCGC GCG^C Cfr10I 5139 GCCGGT [GA]^CCGG[CT] ClaI 6142 ATCGAT AT^CGAT DraIII 927 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 496 GACCAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 2451 TGGCCA [CT]^GGCC[GA] Eco81I 3682 CCTAAGG CC^T[ACGT]AGG EcoRV 2695 GATATC GAT^ATC HhaI 3886 GCGC GCG^C HinP1I 3886 GCGC G^CGC MluNI 2451 TGGCCA TGG^CCA MroI 1513 TCCGGA T^CCGGA MscI 2451 TGGCCA TGG^CCA PflMI 3076 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 6021 GACTAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 6778 ATTAAT AT^TAAT RcaI 2214 TCATGA T^CATGA Van91I 3076 CCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4635 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 2095 GTCTAC GT^[AC][GT]AC 3859 GTAGAC AflIII 2800 ACATGT A^C[GA][CT]GT 6643 ACATGT AlwNI 1723 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 4706 CAGATCCTG AspI 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2940 GACAGAGTC BamHI 3967 GGATCC G^GATCC 4257 GGATCC BanI 264 GGTGCC G^G[CT][GA]CC 2763 GGTGCC BbvCI 315 CCTCAGC CCTCAGC(-5/-2) 4399 CCTCAGC BclI 5460 TGATCA T^GATCA 6426 TGATCA BsaWI 1513 TCCGGA [AT]^CCGG[AT] 1860 ACCGGA BsmI 1110 GAATGC GAATGC(1/-1) 66 GCATTC BspLU11I 2800 ACATGT A^CATGT 6643 ACATGT BspMI 1068 ACCTGC ACCTGC(4/8) 5176 GCAGGT EcoRI 2262 GAATTC G^AATTC 6682 GAATTC EcoT22I 847 ATGCAT ATGCA^T 4867 ATGCAT FbaI 5460 TGATCA T^GATCA 6426 TGATCA NsiI 847 ATGCAT ATGCA^T 4867 ATGCAT PflFI 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2940 GACAGAGTC Tth111I 207 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2940 GACAGAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AflII AhdI AluI AlwI ApoI Asp700I AspEI AvaII BbsI BbvI BfaI BfrI BglII BmyI BpmI BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DsaI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI