Restriction map for
pFN21AE9653
(Linear) (Six-base) MAPSORT of: KIEE9653.seq Check: 3505 from: 1 to: 7026
KIEE9653.orf
With 182 enzymes: SgfI *
March 22, 2012 18:37 ..
AccI GT'mk_AC
Cuts at: 0 2096 3860 7026
Size: 2096 1764 3166
Fragments arranged by size:
3166 2096 1764
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 686 1133 1886 4618 5237 5492 5571
Size: 85 601 447 753 2732 619 255 79
Cuts at: 5571 5989 6290 6389 6988 7026
Size: 418 301 99 599 38
Fragments arranged by size:
2732 753 619 601 599 447 418 301
255 99 85 79 38
AflII C'TTAA_G
Cuts at: 0 859 3280 6133 7026
Size: 859 2421 2853 893
Fragments arranged by size:
2853 2421 893 859
AflIII A'CryG_T
Cuts at: 0 2800 6643 7026
Size: 2800 3843 383
Fragments arranged by size:
3843 2800 383
AhdI GACnn_n'nnGTC
Cuts at: 0 542 1400 1805 7026
Size: 542 858 405 5221
Fragments arranged by size:
5221 858 542 405
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 6085 6344 7026
Size: 6085 259 682
Fragments arranged by size:
6085 682 259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1817 1849 7026
Size: 1817 32 5177
Fragments arranged by size:
5177 1817 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1728 4711 7026
Size: 1728 2983 2315
Fragments arranged by size:
2983 2315 1728
ApaI G_GGCC'C
Cuts at: 0 3157 7026
Size: 3157 3869
ApaLI G'TGCA_C
Cuts at: 0 6697 7026
Size: 6697 329
ApoI r'AATT_y
Cuts at: 0 675 789 921 1428 1568 2262 2587 2615
Size: 675 114 132 507 140 694 325 28
Cuts at: 2615 3640 3814 4515 5981 6180 6682 6986 7026
Size: 1025 174 701 1466 199 502 304 40
Fragments arranged by size:
1466 1025 701 694 675 507 502 325
304 199 174 140 132 114 40 28
AseI AT'TA_AT
Cuts at: 0 6779 7026
Size: 6779 247
AvrII C'CTAG_G
Cuts at: 0 3024 7026
Size: 3024 4002
BamHI G'GATC_C
Cuts at: 0 3967 4257 7026
Size: 3967 290 2769
Fragments arranged by size:
3967 2769 290
BanI G'GyrC_C
Cuts at: 0 264 2763 7026
Size: 264 2499 4263
Fragments arranged by size:
4263 2499 264
BanII G_rGCy'C
Cuts at: 0 3157 7026
Size: 3157 3869
BbsI GAAGACnn'nnnn_
Cuts at: 0 5 66 1478 2681 5795 6021 6646 7026
Size: 5 61 1412 1203 3114 226 625 380
Fragments arranged by size:
3114 1412 1203 625 380 226 61 5
BbvCI CC'TCA_GC
Cuts at: 0 316 4400 7026
Size: 316 4084 2626
Fragments arranged by size:
4084 2626 316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 293 327 7026
Size: 293 34 6699
Fragments arranged by size:
6699 293 34
BclI T'GATC_A
Cuts at: 0 5460 6426 7026
Size: 5460 966 600
Fragments arranged by size:
5460 966 600
BfrBI ATG'CAT
Cuts at: 0 849 4869 7026
Size: 849 4020 2157
Fragments arranged by size:
4020 2157 849
BglII A'GATC_T
Cuts at: 0 1061 4245 4302 6996 7026
Size: 1061 3184 57 2694 30
Fragments arranged by size:
3184 2694 1061 57 30
BlpI GC'TnA_GC
Cuts at: 0 2276 7026
Size: 2276 4750
Bme1580I G_kGCm'C
Cuts at: 0 3157 3381 6701 7026
Size: 3157 224 3320 325
Fragments arranged by size:
3320 3157 325 224
BmrI ACTGGGnnnn_n'
Cuts at: 0 407 924 4072 7026
Size: 407 517 3148 2954
Fragments arranged by size:
3148 2954 517 407
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 999 7026
Size: 999 6027
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3151 3403 4715 4802 4896 5692 5768 6325
Size: 3151 252 1312 87 94 796 76 557
Cuts at: 6325 6973 7026
Size: 648 53
Fragments arranged by size:
3151 1312 796 648 557 252 94 87
76 53
Bpu10I CC'TnA_GC
Cuts at: 0 316 4400 5518 7026
Size: 316 4084 1118 1508
Fragments arranged by size:
4084 1508 1118 316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 5673 7026
Size: 639 5034 1353
Fragments arranged by size:
5034 1353 639
BsaI GGTCTCn'nnnn_
Cuts at: 0 42 970 5148 7026
Size: 42 928 4178 1878
Fragments arranged by size:
4178 1878 928 42
BsaBI GATnn'nnATC
Cuts at: 0 2063 4244 5120 6563 7026
Size: 2063 2181 876 1443 463
Fragments arranged by size:
2181 2063 1443 876 463
BsaWI w'CCGG_w
Cuts at: 0 1513 1860 7026
Size: 1513 347 5166
Fragments arranged by size:
5166 1513 347
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3124 3154 4894 4924 6715 6745 7026
Size: 3124 30 1740 30 1791 30 281
Fragments arranged by size:
3124 1791 1740 281 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1435 1534 2036 3073 6292 6563 6736 7026
Size: 1435 99 502 1037 3219 271 173 290
Fragments arranged by size:
3219 1435 1037 502 290 271 173 99
BseYI C'CCAG_C
Cuts at: 0 6653 7026
Size: 6653 373
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1592 4216 4508 6091 7026
Size: 1592 2624 292 1583 935
Fragments arranged by size:
2624 1592 1583 935 292
BsiHKAI G_wGCw'C
Cuts at: 0 6701 7026
Size: 6701 325
BsmI GAATG_Cn'
Cuts at: 0 66 1116 7026
Size: 66 1050 5910
Fragments arranged by size:
5910 1050 66
Bsp1286I G_dGCh'C
Cuts at: 0 3157 3381 6701 7026
Size: 3157 224 3320 325
Fragments arranged by size:
3320 3157 325 224
BspEI T'CCGG_A
Cuts at: 0 1513 7026
Size: 1513 5513
BspHI T'CATG_A
Cuts at: 0 2214 7026
Size: 2214 4812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1077 5167 7026
Size: 1077 4090 1859
Fragments arranged by size:
4090 1859 1077
BsrDI GCAATG_nn'
Cuts at: 0 266 409 1029 4923 6702 7026
Size: 266 143 620 3894 1779 324
Fragments arranged by size:
3894 1779 620 324 266 143
BsrFI r'CCGG_y
Cuts at: 0 5139 7026
Size: 5139 1887
BsrGI T'GTAC_A
Cuts at: 0 2574 3219 3871 6646 7026
Size: 2574 645 652 2775 380
Fragments arranged by size:
2775 2574 652 645 380
BstAPI GCAn_nnn'nTGC
Cuts at: 0 285 4875 6823 7026
Size: 285 4590 1948 203
Fragments arranged by size:
4590 1948 285 203
BstYI r'GATC_y
Cuts at: 0 1061 2364 3967 4245 4257 4302 4707 5167
Size: 1061 1303 1603 278 12 45 405 460
Cuts at: 5167 6996 7026
Size: 1829 30
Fragments arranged by size:
1829 1603 1303 1061 460 405 278 45
30 12
Bsu36I CC'TnA_GG
Cuts at: 0 3683 7026
Size: 3683 3343
BtgI C'CryG_G
Cuts at: 0 683 1115 1385 3527 7026
Size: 683 432 270 2142 3499
Fragments arranged by size:
3499 2142 683 432 270
BtsI GCAGTG_nn'
Cuts at: 0 261 4228 5949 7026
Size: 261 3967 1721 1077
Fragments arranged by size:
3967 1721 1077 261
ClaI AT'CG_AT
Cuts at: 0 6143 7026
Size: 6143 883
DraI TTT'AAA
Cuts at: 0 635 1575 4502 5001 6737 7026
Size: 635 940 2927 499 1736 289
Fragments arranged by size:
2927 1736 940 635 499 289
DraIII CAC_nnn'GTG
Cuts at: 0 932 7026
Size: 932 6094
DrdI GACnn_nn'nnGTC
Cuts at: 0 502 7026
Size: 502 6524
EaeI y'GGCC_r
Cuts at: 0 2451 7026
Size: 2451 4575
EarI CTCTTCn'nnn_
Cuts at: 0 104 837 1905 2345 5468 5639 6188 6269
Size: 104 733 1068 440 3123 171 549 81
Cuts at: 6269 6365 6476 7026
Size: 96 111 550
Fragments arranged by size:
3123 1068 733 550 549 440 171 111
104 96 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 686 1133 1886 3151 3403 4618 4715
Size: 85 601 447 753 1265 252 1215 97
Cuts at: 4715 4802 4896 5237 5492 5571 5692 5768 5989
Size: 87 94 341 255 79 121 76 221
Cuts at: 5989 6290 6325 6389 6973 6988 7026
Size: 301 35 64 584 15 38
Fragments arranged by size:
1265 1215 753 601 584 447 341 301
255 252 221 121 97 94 87 85
79 76 64 38 35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1073 4439 6718 7026
Size: 1073 3366 2279 308
Fragments arranged by size:
3366 2279 1073 308
EcoO109I rG'GnC_Cy
Cuts at: 0 105 3153 3154 5525 5573 7026
Size: 105 3048 1 2371 48 1453
Fragments arranged by size:
3048 2371 1453 105 48 1
EcoRI G'AATT_C
Cuts at: 0 2262 6682 7026
Size: 2262 4420 344
Fragments arranged by size:
4420 2262 344
EcoRV GAT'ATC
Cuts at: 0 2697 7026
Size: 2697 4329
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 6298 7026
Size: 6298 728
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 198 230 523 528 555 560 601
Size: 27 171 32 293 5 27 5 41
Cuts at: 601 633 1269 1301 1386 1418 1468 1500 1791
Size: 32 636 32 85 32 50 32 291
Cuts at: 1791 1823 3033 3065 3124 3156 4133 4165 4242
Size: 32 1210 32 59 32 977 32 77
Cuts at: 4242 4274 5430 5445 5462 5477 5560 5592 5787
Size: 32 1156 15 17 15 83 32 195
Cuts at: 5787 5819 6315 6347 6411 6443 7026
Size: 32 496 32 64 32 583
Fragments arranged by size:
1210 1156 977 636 583 496 293 291
195 171 85 83 77 64 59 50
41 32 32 32 32 32 32 32
32 32 32 32 32 32 32 27
27 17 15 15 5 5
HindIII A'AGCT_T
Cuts at: 0 637 1145 4348 5881 6598 7026
Size: 637 508 3203 1533 717 428
Fragments arranged by size:
3203 1533 717 637 508 428
MscI TGG'CCA
Cuts at: 0 2453 7026
Size: 2453 4573
MslI CAynn'nnrTG
Cuts at: 0 473 491 553 1390 3326 4530 4688 6160
Size: 473 18 62 837 1936 1204 158 1472
Cuts at: 6160 7026
Size: 866
Fragments arranged by size:
1936 1472 1204 866 837 473 158 62
18
MspA1I CmG'CkG
Cuts at: 0 276 1606 2056 6261 7026
Size: 276 1330 450 4205 765
Fragments arranged by size:
4205 1330 765 450 276
NcoI C'CATG_G
Cuts at: 0 1115 1385 3527 7026
Size: 1115 270 2142 3499
Fragments arranged by size:
3499 2142 1115 270
NdeI CA'TA_TG
Cuts at: 0 218 4531 5322 7026
Size: 218 4313 791 1704
Fragments arranged by size:
4313 1704 791 218
NsiI A_TGCA'T
Cuts at: 0 851 4871 7026
Size: 851 4020 2155
Fragments arranged by size:
4020 2155 851
NspI r_CATG'y
Cuts at: 0 490 853 2804 6647 7026
Size: 490 363 1951 3843 379
Fragments arranged by size:
3843 1951 490 379 363
PciI A'CATG_T
Cuts at: 0 2800 6643 7026
Size: 2800 3843 383
Fragments arranged by size:
3843 2800 383
PflMI CCAn_nnn'nTGG
Cuts at: 0 3082 7026
Size: 3082 3944
PfoI T'CCnGG_A
Cuts at: 0 3075 3228 6506 6631 7026
Size: 3075 153 3278 125 395
Fragments arranged by size:
3278 3075 395 153 125
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 7026
Size: 814 32 6180
Fragments arranged by size:
6180 814 32
PpuMI rG'GwC_Cy
Cuts at: 0 105 5525 5573 7026
Size: 105 5420 48 1453
Fragments arranged by size:
5420 1453 105 48
PshAI GACnn'nnGTC
Cuts at: 0 6025 7026
Size: 6025 1001
PsiI TTA'TAA
Cuts at: 0 1850 4225 6758 7026
Size: 1850 2375 2533 268
Fragments arranged by size:
2533 2375 1850 268
PspOMI G'GGCC_C
Cuts at: 0 3153 7026
Size: 3153 3873
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 6293 6325 7026
Size: 6293 32 701
Fragments arranged by size:
6293 701 32
PstI C_TGCA'G
Cuts at: 0 256 1218 4581 6243 7026
Size: 256 962 3363 1662 783
Fragments arranged by size:
3363 1662 962 783 256
PvuII CAG'CTG
Cuts at: 0 276 1606 2056 6261 7026
Size: 276 1330 450 4205 765
Fragments arranged by size:
4205 1330 765 450 276
SapI GCTCTTCn'nnn_
Cuts at: 0 1905 2345 6365 7026
Size: 1905 440 4020 661
Fragments arranged by size:
4020 1905 661 440
SfcI C'TryA_G
Cuts at: 0 252 1214 2903 3158 3731 4577 4883 6239
Size: 252 962 1689 255 573 846 306 1356
Cuts at: 6239 7026
Size: 787
Fragments arranged by size:
1689 1356 962 846 787 573 306 255
252
SmlI C'TyrA_G
Cuts at: 0 654 859 3280 5652 6133 7026
Size: 654 205 2421 2372 481 893
Fragments arranged by size:
2421 2372 893 654 481 205
SspI AAT'ATT
Cuts at: 0 77 885 948 1301 4123 4506 4608 4899
Size: 77 808 63 353 2822 383 102 291
Cuts at: 4899 7026
Size: 2127
Fragments arranged by size:
2822 2127 808 383 353 291 102 77
63
StuI AGG'CCT
Cuts at: 0 569 2889 3868 5287 5974 7026
Size: 569 2320 979 1419 687 1052
Fragments arranged by size:
2320 1419 1052 979 687 569
StyI C'CwwG_G
Cuts at: 0 88 812 1115 1385 3024 3527 6039 6714
Size: 88 724 303 270 1639 503 2512 675
Cuts at: 6714 7026
Size: 312
Fragments arranged by size:
2512 1639 724 675 503 312 303 270
88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 943 3658 4542 7026
Size: 943 2715 884 2484
Fragments arranged by size:
2715 2484 943 884
TatI w'GTAC_w
Cuts at: 0 2250 2574 3046 3219 3871 4009 6646 7026
Size: 2250 324 472 173 652 138 2637 380
Fragments arranged by size:
2637 2250 652 472 380 324 173 138
Tth111I GACn'n_nGTC
Cuts at: 0 210 2943 7026
Size: 210 2733 4083
Fragments arranged by size:
4083 2733 210
XbaI T'CTAG_A
Cuts at: 0 4635 7026
Size: 4635 2391
XmnI GAAnn'nnTTC
Cuts at: 0 128 347 1943 4187 4607 5096 5222 5387
Size: 128 219 1596 2244 420 489 126 165
Cuts at: 5387 7026
Size: 1639
Fragments arranged by size:
2244 1639 1596 489 420 219 165 128
126
Enzymes that do cut:
AccI AcuI AflII AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI ApoI AseI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstYI Bsu36I
BtgI BtsI ClaI DraI DraIII DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HindIII
MscI MslI MspA1I NcoI NdeI NsiI NspI PciI
PflMI PfoI PpiI PpuMI PshAI PsiI PspOMI PsrI
PstI PvuII SapI SfcI SmlI SspI StuI StyI
TaqII TatI Tth111I XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AleI AscI
AsiSI AvaI BaeI BbeI BciVI BglI BmgBI BmtI
BsaAI BsaHI BsiEI BsiWI BsmBI BsrBI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI CspCI EagI EciI
EcoICRI FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NgoMIV NheI
NotI NruI PacI PasI PmeI PmlI PspXI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TstI XcmI XhoI XmaI ZraI