Restriction map for
pFN21AE0477
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# NO CUT ENZYMES ( 158 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AspEI AspHI AvaI AvrII BalI
BamHI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoT22I FauI FbaI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AlwNI 38 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 522 ATTAAT AT^TAAT
AsnI 522 ATTAAT AT^TAAT
AspI 75 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 23 TGCGCA TGC^GCA
BmyI 32 GTGCCC G[AGT]GC[ACT]^C
BsmI 230 GCATTC GAATGC(1/-1)
Bsp1286I 32 GTGCCC G[AGT]GC[ACT]^C
BsrDI 743 CATTGC GCAATG(2/0)
BssHII 5 GCGCGC G^CGCGC
BstEII 598 GGTTACC G^GT[ACGT]ACC
BstF5I 366 GGATG GGATG(2/0)
BstNI 603 CCTGG CC^[AT]GG
BstPI 598 GGTTACC G^GT[ACGT]ACC
BstYI 473 AGATCC [GA]^GATC[CT]
EaeI 120 CGGCCA [CT]^GGCC[GA]
EcoO65I 598 GGTTACC G^GT[ACGT]ACC
EcoRI 682 GAATTC G^AATTC
EcoRII 603 CCTGG ^CC[AT]GG
EcoRV 795 GATATC GAT^ATC
FokI 366 GGATG GGATG(9/13)
FspI 23 TGCGCA TGC^GCA
MaeII 788 ACGT A^CGT
MflI 473 AGATCC [GA]^GATC[CT]
MspA1I 41 CCGCTG C[AC]G^C[GT]G
MvaI 603 CCTGG CC^[AT]GG
PflFI 75 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 522 ATTAAT AT^TAAT
SapI 886 GAAGAGC GCTCTTC(1/4)
SmlI 821 CTCAAG C^T[CT][GA]AG
Tsp45I 170 GTCAC ^GT[GC]AC
Tth111I 75 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 473 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
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Acc65I 427 GGTACC G^GTACC
639 GGTACC
AcsI 482 GAATTT [GA]^AATT[CT]
682 GAATTC
AlwI 474 GATCC GGATC(4/5)
544 GATCC
ApoI 482 GAATTT [GA]^AATT[CT]
682 GAATTC
Asp700I 390 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
457 GAAGTTATTC
Asp718I 427 GGTACC G^GTACC
639 GGTACC
AvaII 62 GGACC G^G[AT]CC
74 GGACC
BcnI 77 CCGGG CC^[GC]GG
595 CCGGG
BseRI 112 GAGGAG GAGGAG(10/8)
767 GAGGAG
BsgI 68 GTGCAG GTGCAG(16/14)
790 GTGCAG
BsmFI 61 GGGAC GGGAC(10/14)
73 GGGAC
BspHI 189 TCATGA T^CATGA
564 TCATGA
DdeI 110 CTGAG C^T[ACGT]AG
437 CTCAG
EarI 148 CTCTTC CTCTTC(1/4)
886 GAAGAG
HaeIII 96 GGCC GG^CC
121 GGCC
HapII 77 CCGG C^CGG
595 CCGG
HpaII 77 CCGG C^CGG
595 CCGG
KpnI 427 GGTACC GGTAC^C
639 GGTACC
Ksp632I 148 CTCTTC CTCTTC(1/4)
886 GAAGAG
MslI 231 CATTCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CACAAACGTG
MspI 77 CCGG C^CGG
595 CCGG
NciI 77 CCGGG CC^[GC]GG
595 CCGGG
RcaI 189 TCATGA T^CATGA
564 TCATGA
TspRI 66 CAGTG CA[GC]TG[ACGT][ACGT]^
108 CACTG
XmnI 390 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
457 GAAGTTATTC
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BbvI BsaJI BsiYI BslI BsrI
BssKI BstUI Cac8I CfoI Cfr13I
DpnII EcoT14I Fnu4HI HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvnI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI StyI TfiI Tru9I
TseI Tsp509I