Restriction map for
pFN21AE0477
(Linear) (Six-base) MAPSORT of: KIEE0477.seq Check: 5793 from: 1 to: 915
KIEE0477.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 427 639 915
Size: 427 212 276
Fragments arranged by size:
427 276 212
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 569 915
Size: 223 346 346
Fragments arranged by size:
346 346 223
AleI CACnn'nnGTG
Cuts at: 0 787 915
Size: 787 128
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 805 915
Size: 805 110
AlwNI CAG_nnn'CTG
Cuts at: 0 43 915
Size: 43 872
ApoI r'AATT_y
Cuts at: 0 482 682 915
Size: 482 200 233
Fragments arranged by size:
482 233 200
AseI AT'TA_AT
Cuts at: 0 523 915
Size: 523 392
BanI G'GyrC_C
Cuts at: 0 31 427 639 915
Size: 31 396 212 276
Fragments arranged by size:
396 276 212 31
Bme1580I G_kGCm'C
Cuts at: 0 36 915
Size: 36 879
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 915
Size: 806 109
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 782 915
Size: 127 655 133
Fragments arranged by size:
655 133 127
BseYI C'CCAG_C
Cuts at: 0 36 915
Size: 36 879
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 811 915
Size: 89 722 104
Fragments arranged by size:
722 104 89
BsmI GAATG_Cn'
Cuts at: 0 230 915
Size: 230 685
Bsp1286I G_dGCh'C
Cuts at: 0 36 915
Size: 36 879
BspHI T'CATG_A
Cuts at: 0 189 564 915
Size: 189 375 351
Fragments arranged by size:
375 351 189
BsrDI GCAATG_nn'
Cuts at: 0 742 915
Size: 742 173
BssHII G'CGCG_C
Cuts at: 0 5 915
Size: 5 910
BstEII G'GTnAC_C
Cuts at: 0 598 915
Size: 598 317
BstYI r'GATC_y
Cuts at: 0 473 915
Size: 473 442
EaeI y'GGCC_r
Cuts at: 0 120 915
Size: 120 795
EarI CTCTTCn'nnn_
Cuts at: 0 154 881 915
Size: 154 727 34
Fragments arranged by size:
727 154 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 569 915
Size: 223 346 346
Fragments arranged by size:
346 346 223
EcoRI G'AATT_C
Cuts at: 0 682 915
Size: 682 233
EcoRV GAT'ATC
Cuts at: 0 797 915
Size: 797 118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 631 763 915
Size: 631 132 152
Fragments arranged by size:
631 152 132
FspI TGC'GCA
Cuts at: 0 25 915
Size: 25 890
KpnI G_GTAC'C
Cuts at: 0 431 643 915
Size: 431 212 272
Fragments arranged by size:
431 272 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 915
Size: 715 200
MslI CAynn'nnrTG
Cuts at: 0 235 787 915
Size: 235 552 128
Fragments arranged by size:
552 235 128
MspA1I CmG'CkG
Cuts at: 0 43 915
Size: 43 872
SapI GCTCTTCn'nnn_
Cuts at: 0 881 915
Size: 881 34
SmlI C'TyrA_G
Cuts at: 0 821 915
Size: 821 94
StyI C'CwwG_G
Cuts at: 0 329 477 906 915
Size: 329 148 429 9
Fragments arranged by size:
429 329 148 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 915
Size: 163 32 720
Fragments arranged by size:
720 163 32
Tth111I GACn'n_nGTC
Cuts at: 0 78 915
Size: 78 837
XmnI GAAnn'nnTTC
Cuts at: 0 394 461 915
Size: 394 67 454
Fragments arranged by size:
454 394 67
Enzymes that do cut:
Acc65I AcuI AleI AlfI AlwNI ApoI AseI BanI
Bme1580I BpuEI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BsrDI BssHII BstEII BstYI EaeI EarI Eco57MI EcoRI
EcoRV FalI FspI KpnI MmeI MslI MspA1I SapI
SmlI StyI TstI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AloI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BanII BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoO109I FseI FspAI HaeII
Hin4I HincII HindIII HpaI KasI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI XbaI XcmI XhoI XmaI ZraI