################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccI AccII AccIII Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BcgI BciVI BlnI BseAI BsiEI BsiWI BsmBI BsmI BspEI BspHI BssHII Bst1107I BstBI BstUI BstZ17I CpoI DraI EagI EclXI Eco47III Eco52I EcoT22I FseI FspI HpaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PflFI PmeI PshBI PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### AcyI 3 GGCGTC G[GA]^CG[CT]C AgeI 3494 ACCGGT A^CCGGT BalI 3383 TGGCCA TGG^CCA BbrPI 472 CACGTG CAC^GTG BsaHI 3 GGCGTC G[GA]^CG[CT]C BspDI 3265 ATCGAT AT^CGAT BsrFI 3494 ACCGGT [GA]^CCGG[CT] BssSI 812 CTCGTG CACGAG(-5/-1) BstEII 4580 GGTCACC G^GT[ACGT]ACC BstPI 4580 GGTCACC G^GT[ACGT]ACC BstXI 2546 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 3494 ACCGGT [GA]^CCGG[CT] ClaI 3265 ATCGAT AT^CGAT EcoO65I 4580 GGTCACC G^GT[ACGT]ACC EcoRV 3628 GATATC GAT^ATC Hin1I 3 GGCGTC G[GA]^CG[CT]C HincII 4006 GTCAAC GT[CT]^[GA]AC HindII 4006 GTCAAC GT[CT]^[GA]AC MluNI 3383 TGGCCA TGG^CCA MscI 3383 TGGCCA TGG^CCA NheI 5190 GCTAGC G^CTAGC PinAI 3494 ACCGGT A^CCGGT PmaCI 472 CACGTG CAC^GTG PmlI 472 CACGTG CAC^GTG PshAI 2259 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScaI 4215 AGTACT AGT^ACT SexAI 2990 ACCAGGT A^CC[AT]GGT SphI 4529 GCATGC GCATG^C ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AclI 4115 AACGTT AA^CGTT 5060 AACGTT AflII 3022 CTTAAG C^TTAAG 3163 CTTAAG AhdI 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4060 GACAACCAGTC AocI 4282 CCTCAGG CC^T[ACGT]AGG 4813 CCTGAGG Asp700I 2433 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4338 GAAAGAGTTC AspEI 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4060 GACAACCAGTC BamHI 1170 GGATCC G^GATCC 3906 GGATCC BbvCI 4543 GCTGAGG CCTCAGC(-5/-2) 5446 GCTGAGG BclI 1901 TGATCA T^GATCA 2540 TGATCA BfrI 3022 CTTAAG C^TTAAG 3163 CTTAAG BsaAI 472 CACGTG [CT]AC^GT[GA] 1458 TACGTG BsaWI 3461 ACCGGA [AT]^CCGG[AT] 3494 ACCGGT Bsp1407I 2253 TGTACA T^GTACA 4759 TGTACA BsrGI 2253 TGTACA T^GTACA 4759 TGTACA Bsu36I 4282 CCTCAGG CC^T[ACGT]AGG 4813 CCTGAGG DraIII 1867 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 3320 CACCGGGTG DrdI 2377 GACATGTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3504 GACCCGTGCGTC EaeI 1591 CGGCCA [CT]^GGCC[GA] 3383 TGGCCA Eam1105I 4048 GACGACCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4060 GACAACCAGTC Eco81I 4282 CCTCAGG CC^T[ACGT]AGG 4813 CCTGAGG EcoRI 1327 GAATTC G^AATTC 5609 GAATTC FbaI 1901 TGATCA T^GATCA 2540 TGATCA HindIII 334 AAGCTT A^AGCTT 2056 AAGCTT NcoI 5208 CCATGG C^CATGG 5423 CCATGG NdeI 1321 CATATG CA^TATG 4352 CATATG PaeR7I 903 CTCGAG C^TCGAG 3356 CTCGAG Psp1406I 4115 AACGTT AA^CGTT 5060 AACGTT PstI 1660 CTGCAG CTGCA^G 3142 CTGCAG SacI 3455 GAGCTC GAGCT^C 3810 GAGCTC SapI 357 GAAGAGC GCTCTTC(1/4) 5122 GAAGAGC SspBI 2253 TGTACA T^GTACA 4759 TGTACA XcmI 575 CCAAGATGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5486 CCAAGGAGTCAATGG XhoI 903 CTCGAG C^TCGAG 3356 CTCGAG XmnI 2433 GAATGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4338 GAAAGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrBI BsrDI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII