Restriction map for
pFN21AE9640
(Circular) (Six-base) MAPSORT of: pFN21AE9640.seq Check: 2790 from: 1 to: 10420
pFN21AE9640 10420 bp
With 182 enzymes: SgfI *
March 28, 2012 12:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5530 6667 5530
Size: 1137 9283
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 8230 278
Size: 53 83 186 936 6694 2468
Fragments arranged by size:
6694 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 7763 1937
Size: 5826 4594
Acc65I G'GTAC_C
Cuts at: 7741 7741
Size: 10420
AclI AA'CG_TT
Cuts at: 1563 6121 7066 8547 8920 1563
Size: 4558 945 1481 373 3063
Fragments arranged by size:
4558 3063 1481 945 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2509 3497 4637 5799 6075 6684 6989 8483
Size: 877 988 1140 1162 276 609 305 1494
Cuts at: 8483 9867 10135 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1494 1384 1162 1140 988 877 609
305 276 268
AflII C'TTAA_G
Cuts at: 820 1017 5027 5168 820
Size: 197 4010 141 6072
Fragments arranged by size:
6072 4010 197 141
AflIII A'CryG_T
Cuts at: 1203 2478 3464 4383 4842 6514 7401 9319 10296
Size: 1275 986 919 459 1672 887 1918 977
Cuts at: 10296 1203
Size: 1327
Fragments arranged by size:
1918 1672 1327 1275 986 977 919 887
459
AgeI A'CCGG_T
Cuts at: 5499 9251 5499
Size: 3752 6668
AhdI GACnn_n'nnGTC
Cuts at: 6058 6070 9149 6058
Size: 12 3079 7329
Fragments arranged by size:
7329 3079 12
AleI CACnn'nnGTG
Cuts at: 1166 3536 5540 1166
Size: 2370 2004 6046
Fragments arranged by size:
6046 2370 2004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 10420
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4868 4900 4868
Size: 32 10388
AlwNI CAG_nnn'CTG
Cuts at: 2446 2714 3557 6746 7486 7628 8163 9735 2446
Size: 268 843 3189 740 142 535 1572 3131
Fragments arranged by size:
3189 3131 1572 843 740 535 268 142
ApaLI G'TGCA_C
Cuts at: 8477 9633 8477
Size: 1156 9264
ApoI r'AATT_y
Cuts at: 1493 3332 3794 4514 4960 5642 7322 7614 7729
Size: 1839 462 720 446 682 1680 292 115
Cuts at: 7729 7955 8107 10216 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1839 1697 1680 720 682 462 446
292 226 152 115
AseI AT'TA_AT
Cuts at: 160 1032 8974 160
Size: 872 7942 1606
Fragments arranged by size:
7942 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
AvaI C'yCGr_G
Cuts at: 1870 1943 2184 2908 3197 3839 4745 4980 5361
Size: 73 241 724 289 642 906 235 381
Cuts at: 5361 6432 7745 1870
Size: 1071 1313 4545
Fragments arranged by size:
4545 1313 1071 906 724 642 381 289
241 235 73
BamHI G'GATC_C
Cuts at: 3175 5911 7750 8117 3175
Size: 2736 1839 367 5478
Fragments arranged by size:
5478 2736 1839 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2746 5940 7681 7741 9196
Size: 325 217 627 959 3194 1741 60 1455
Cuts at: 9196 618
Size: 1842
Fragments arranged by size:
3194 1842 1741 1455 959 627 325 217
60
BanII G_rGCy'C
Cuts at: 1391 2024 4210 4378 4574 4922 5464 5597 5819
Size: 633 2186 168 196 348 542 133 222
Cuts at: 5819 6888 7739 1391
Size: 1069 851 4072
Fragments arranged by size:
4072 2186 1069 851 633 542 348 222
196 168 133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 2472 4264 4461 5787 6114 6963
Size: 971 558 15 1792 197 1326 327 849
Cuts at: 6963 7160 7416 928
Size: 197 256 3932
Fragments arranged by size:
3932 1792 1326 971 849 558 327 256
197 197 15
BbvCI CC'TCA_GC
Cuts at: 6549 7452 6549
Size: 903 9517
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 8611 8645 1903
Size: 34 6674 34 3678
Fragments arranged by size:
6674 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8312 9522 8312
Size: 1210 9210
BclI T'GATC_A
Cuts at: 1548 3906 4545 1548
Size: 2358 639 7423
Fragments arranged by size:
7423 2358 639
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3117 3604 4038
Size: 107 122 71 631 605 1445 487 434
Cuts at: 4038 9031 136
Size: 4993 1525
Fragments arranged by size:
4993 1525 1445 631 605 487 434 122
107 71
BglII A'GATC_T
Cuts at: 5965 6934 7164 10415 5965
Size: 969 230 3251 5970
Fragments arranged by size:
5970 3251 969 230
BlpI GC'TnA_GC
Cuts at: 2439 4916 5334 7837 2439
Size: 2477 418 2503 5022
Fragments arranged by size:
5022 2503 2477 418
Bme1580I G_kGCm'C
Cuts at: 1790 4217 5116 7684 8481 9637 1790
Size: 2427 899 2568 797 1156 2573
Fragments arranged by size:
2573 2568 2427 1156 899 797
BmgBI CAC'GTC
Cuts at: 1318 3895 1318
Size: 2577 7843
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2464 4360 4695
Size: 416 53 83 399 24 1047 1896 335
Cuts at: 4695 5295 5827 5932 6138 6301 7616 8151 9109
Size: 600 532 105 206 163 1315 535 958
Cuts at: 9109 442
Size: 1753
Fragments arranged by size:
1896 1753 1315 1047 958 600 535 532
416 399 335 206 163 105 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 7199 1056
Size: 6143 4277
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4347 7126 4347
Size: 2779 7641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3534 3692 4068 4362 4649 6550 6603 9080
Size: 2295 158 376 294 287 1901 53 2477
Cuts at: 9080 1239
Size: 2579
Fragments arranged by size:
2579 2477 2295 1901 376 294 287 158
53
Bpu10I CC'TnA_GC
Cuts at: 4209 6549 7452 4209
Size: 2340 903 7177
Fragments arranged by size:
7177 2340 903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 4034 5144 8545 9410 9708 2141
Size: 1893 1110 3401 865 298 2853
Fragments arranged by size:
3401 2853 1893 1110 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3294 6315 6404 7010 9083 882
Size: 617 1795 3021 89 606 2073 2219
Fragments arranged by size:
3021 2219 2073 1795 617 606 89
BsaAI yAC'GTr
Cuts at: 493 1204 2479 3465 8211 493
Size: 711 1275 986 4746 2702
Fragments arranged by size:
4746 2702 1275 986 711
BsaBI GATnn'nnATC
Cuts at: 3500 3558 3851 10414 3500
Size: 58 293 6563 3506
Fragments arranged by size:
6563 3506 293 58
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 8227 8609 9244
Size: 53 83 186 936 476 6218 382 635
Cuts at: 9244 275
Size: 1451
Fragments arranged by size:
6218 1451 936 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5466 5499 8853 9251 9525 9672 10083 5466
Size: 33 3354 398 274 147 411 5803
Fragments arranged by size:
5803 3354 411 398 274 147 33
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2131 2161 3557 3587 4346
Size: 30 165 30 728 30 1396 30 759
Cuts at: 4346 4376 4868 4898 6908 6938 1178
Size: 30 492 30 2010 30 4660
Fragments arranged by size:
4660 2010 1396 759 728 492 165 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2069 2279 2282 2399 2402 2405 2408
Size: 880 3 210 3 117 3 3 3
Cuts at: 2408 2504 3065 3068 3290 3326 4793 5540 5700
Size: 96 561 3 222 36 1467 747 160
Cuts at: 5700 5703 5885 6177 6842 1186
Size: 3 182 292 665 4764
Fragments arranged by size:
4764 1467 880 747 665 561 292 222
210 182 160 117 96 36 3 3
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2227 2875 4831 5366 5590 6576 7597 7710 9623
Size: 648 1956 535 224 986 1021 113 1913
Cuts at: 9623 10119 2227
Size: 496 2528
Fragments arranged by size:
2528 1956 1913 1021 986 648 535 496
224 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3509 3662 4145 6630 7517 1163
Size: 573 1773 153 483 2485 887 4066
Fragments arranged by size:
4066 2485 1773 887 573 483 153
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7870 8631 9659 1540
Size: 275 186 5869 761 1028 2301
Fragments arranged by size:
5869 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5335 5464 5819 8481 8566 9637 5335
Size: 129 355 2662 85 1071 6118
Fragments arranged by size:
6118 2662 1071 355 129 85
BsmI GAATG_Cn'
Cuts at: 7931 8024 7931
Size: 93 10327
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2024 4210 4217 4378 4574 4922 5116
Size: 399 234 2186 7 161 196 348 194
Cuts at: 5116 5335 5464 5597 5819 6888 7684 7739 8481
Size: 219 129 133 222 1069 796 55 742
Cuts at: 8481 8566 9637 1391
Size: 85 1071 2174
Fragments arranged by size:
2186 2174 1071 1069 796 742 399 348
234 222 219 196 194 161 133 129
85 55 7
BspEI T'CCGG_A
Cuts at: 10083 10083
Size: 10420
BspHI T'CATG_A
Cuts at: 8309 10039 8309
Size: 1730 8690
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5232 5530 6333 6667 7693 7708 7775 844
Size: 4388 298 803 334 1026 15 67 3489
Fragments arranged by size:
4388 3489 1026 803 334 298 67 15
BsrBI CCG'CTC
Cuts at: 1394 1823 3604 4357 7132 8307 1394
Size: 429 1781 753 2775 1175 3507
Fragments arranged by size:
3507 2775 1781 1175 753 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2547 4791 5076 6179 7080 7605 8915
Size: 1183 1299 2244 285 1103 901 525 1310
Cuts at: 8915 9089 65
Size: 174 1396
Fragments arranged by size:
2244 1396 1310 1299 1183 1103 901 525
285 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5499 9064 9251 1700
Size: 110 3689 3565 187 2869
Fragments arranged by size:
3689 3565 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4258 6764 96
Size: 4162 2506 3752
Fragments arranged by size:
4162 3752 2506
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10420
BssSI C'ACGA_G
Cuts at: 2817 8480 9492 2817
Size: 5663 1012 3745
Fragments arranged by size:
5663 3745 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4828 4846 4976 5155 5330 794
Size: 4034 18 130 179 175 5884
Fragments arranged by size:
5884 4034 179 175 130 18
BstBI TT'CG_AA
Cuts at: 9224 9224
Size: 10420
BstEII G'GTnAC_C
Cuts at: 1184 6585 1184
Size: 5401 5019
BstXI CCAn_nnnn'nTGG
Cuts at: 4558 4558
Size: 10420
BstYI r'GATC_y
Cuts at: 1972 2903 3175 3279 3429 3495 3963 5023 5606
Size: 931 272 104 150 66 468 1060 583
Cuts at: 5606 5911 5965 6934 7164 7750 8117 8502 8519
Size: 305 54 969 230 586 367 385 17
Cuts at: 8519 9971 10057 10069 10415 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1060 969 931 586 583 468
385 367 346 305 272 230 150 104
86 66 54 17 12
Bsu36I CC'TnA_GG
Cuts at: 6288 6819 6288
Size: 531 9889
BtgI C'CryG_G
Cuts at: 513 1065 2004 7213 7294 7428 513
Size: 552 939 5209 81 134 3505
Fragments arranged by size:
5209 3505 939 552 134 81
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 9681
Fragments arranged by size:
9681 683 56
BtsI GCAGTG_nn'
Cuts at: 2053 3220 3403 3643 5116 5391 5722 7936 8730
Size: 1167 183 240 1473 275 331 2214 794
Cuts at: 8730 8750 2053
Size: 20 3723
Fragments arranged by size:
3723 2214 1473 1167 794 331 275 240
183 20
ClaI AT'CG_AT
Cuts at: 1554 5271 1554
Size: 3717 6703
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2701 2736 3337 3372 529
Size: 35 2137 35 601 35 7577
Fragments arranged by size:
7577 2137 601 35 35 35
DraI TTT'AAA
Cuts at: 7724 7724
Size: 10420
DraIII CAC_nnn'GTG
Cuts at: 3877 5330 3877
Size: 1453 8967
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4388 5515 9427 809
Size: 507 3072 1127 3912 1802
Fragments arranged by size:
3912 3072 1802 1127 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3596 5388 7867
Size: 54 1425 50 275 18 1766 1792 2479
Cuts at: 7867 8756 10385 8
Size: 889 1629 43
Fragments arranged by size:
2479 1792 1766 1629 1425 889 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7867 1537
Size: 275 6055 4090
Fragments arranged by size:
6055 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2106 2118 2268 2357 3282 3303 3753 4707
Size: 753 12 150 89 925 21 450 954
Cuts at: 4707 5060 5862 6699 6960 6978 7122 8350 1353
Size: 353 802 837 261 18 144 1228 3423
Fragments arranged by size:
3423 1228 954 925 837 802 753 450
353 261 150 144 89 21 18 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7484 9007 9381 9527 387
Size: 1064 138 5895 1523 374 146 1280
Fragments arranged by size:
5895 1523 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5462 5817 5462
Size: 355 10065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2509 3497 3534 3692 4068 4362 4637
Size: 393 877 988 37 158 376 294 275
Cuts at: 4637 4649 5799 6075 6550 6603 6684 6989 8483
Size: 12 1150 276 475 53 81 305 1494
Cuts at: 8483 9080 9867 10135 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1494 1150 988 877 787 597 475
393 376 305 294 276 275 268 158
81 53 37 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3287 3308 4289 5171 5228 7490 3287
Size: 21 981 882 57 2262 6217
Fragments arranged by size:
6217 2262 981 882 57 21
EcoO109I rG'GnC_Cy
Cuts at: 2116 2188 2783 3787 4180 4310 5371 6154 6772
Size: 72 595 1004 393 130 1061 783 618
Cuts at: 6772 7027 2116
Size: 255 5509
Fragments arranged by size:
5509 1061 1004 783 618 595 393 255
130 72
EcoRI G'AATT_C
Cuts at: 3332 7614 7729 8107 3332
Size: 4282 115 378 5645
Fragments arranged by size:
5645 4282 378 115
EcoRV GAT'ATC
Cuts at: 5635 5635
Size: 10420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6272 6272
Size: 10420
FspI TGC'GCA
Cuts at: 8926 8926
Size: 10420
HaeII r_GCGC'y
Cuts at: 1678 2215 4329 6529 9567 1678
Size: 537 2114 2200 3038 2531
Fragments arranged by size:
3038 2531 2200 2114 537
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2117 2149 2414 2446 2921
Size: 32 135 32 545 32 265 32 475
Cuts at: 2921 2953 6044 6056 6076 6088 6719 6751 6908
Size: 32 3091 12 20 12 631 32 157
Cuts at: 6908 6940 7156 7188 9061 9093 9135 9167 1373
Size: 32 216 32 1873 32 42 32 2626
Fragments arranged by size:
3091 2626 1873 631 545 475 265 216
157 135 42 32 32 32 32 32
32 32 32 32 32 20 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 6013 7764 8010 669
Size: 988 281 4075 1751 246 3079
Fragments arranged by size:
4075 3079 1751 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2339 4061 748
Size: 1591 1722 7107
Fragments arranged by size:
7107 1722 1591
HpaI GTT'AAC
Cuts at: 8010 8010
Size: 10420
KpnI G_GTAC'C
Cuts at: 7745 7745
Size: 10420
MfeI C'AATT_G
Cuts at: 8019 8019
Size: 10420
MluI A'CGCG_T
Cuts at: 10296 10296
Size: 10420
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4053 4130 6087 9534 9718 1305
Size: 2748 77 1957 3447 184 2007
Fragments arranged by size:
3447 2748 2007 1957 184 77
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5390 10
Size: 54 1425 343 3558 5040
Fragments arranged by size:
5040 3558 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3536 4556 5540 5730 5946 6384 6519
Size: 648 2370 1020 984 190 216 438 135
Cuts at: 6519 7218 8378 8737 8896 518
Size: 699 1160 359 159 2042
Fragments arranged by size:
2370 2042 1160 1020 984 699 648 438
359 216 190 159 135
MspA1I CmG'CkG
Cuts at: 1606 2666 2681 3670 3709 7378 7552 7836 8513
Size: 1060 15 989 39 3669 174 284 677
Cuts at: 8513 9661 9906 1606
Size: 1148 245 2120
Fragments arranged by size:
3669 2120 1148 1060 989 677 284 245
174 39 15
NcoI C'CATG_G
Cuts at: 513 1065 2004 7213 7428 513
Size: 552 939 5209 215 3505
Fragments arranged by size:
5209 3505 939 552 215
NdeI CA'TA_TG
Cuts at: 387 3327 6358 387
Size: 2940 3031 4449
Fragments arranged by size:
4449 3031 2940
NheI G'CTAG_C
Cuts at: 1052 7195 1052
Size: 6143 4277
NotI GC'GGCC_GC
Cuts at: 7867 7867
Size: 10420
NspI r_CATG'y
Cuts at: 1132 1229 3634 4387 4831 4846 4882 5101 5296
Size: 97 2405 753 444 15 36 219 195
Cuts at: 5296 6458 6518 6538 7405 7778 9323 1132
Size: 1162 60 20 867 373 1545 2229
Fragments arranged by size:
2405 2229 1545 1162 867 753 444 373
219 195 97 60 36 20 15
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 10420
PciI A'CATG_T
Cuts at: 4383 4842 6514 7401 9319 4383
Size: 459 1672 887 1918 5484
Fragments arranged by size:
5484 1918 1672 887 459
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3328 3737 3877 4762 5839 1108
Size: 2220 409 140 885 1077 5689
Fragments arranged by size:
5689 2220 1077 885 409 140
PfoI T'CCnGG_A
Cuts at: 2445 3282 3966 2445
Size: 837 684 8899
Fragments arranged by size:
8899 837 684
PmeI GTTT'AAAC
Cuts at: 7724 7724
Size: 10420
PmlI CAC'GTG
Cuts at: 2479 2479
Size: 10420
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3593 3625 4427 4459 4868 4900 6332 6364 8489
Size: 32 802 32 409 32 1432 32 2125
Cuts at: 8489 8521 10002 10034 3593
Size: 32 1481 32 3979
Fragments arranged by size:
3979 2125 1481 1432 802 409 32 32
32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2116 2188 2783 3787 7027 2116
Size: 72 595 1004 3240 5509
Fragments arranged by size:
5509 3240 1004 595 72
PshAI GACnn'nnGTC
Cuts at: 1613 4268 1613
Size: 2655 7765
PsiI TTA'TAA
Cuts at: 5256 7208 7990 5256
Size: 1952 782 7686
Fragments arranged by size:
7686 1952 782
PspXI vC'TCGA_Gb
Cuts at: 2908 5361 2908
Size: 2453 7967
PstI C_TGCA'G
Cuts at: 830 3669 5151 7772 830
Size: 2839 1482 2621 3478
Fragments arranged by size:
3478 2839 2621 1482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10420
PvuII CAG'CTG
Cuts at: 2666 3670 7378 7552 2666
Size: 1004 3708 174 5534
Fragments arranged by size:
5534 3708 1004 174
SacI G_AGCT'C
Cuts at: 5464 5819 5464
Size: 355 10065
SalI G'TCGA_C
Cuts at: 1936 7762 1936
Size: 5826 4594
SanDI GG'GwC_CC
Cuts at: 2188 2188
Size: 10420
SapI GCTCTTCn'nnn_
Cuts at: 2357 7122 2357
Size: 4765 5655
SbfI CC_TGCA'GG
Cuts at: 7772 7772
Size: 10420
ScaI AGT'ACT
Cuts at: 6222 8668 10380 6222
Size: 2446 1712 6262
Fragments arranged by size:
6262 2446 1712
SexAI A'CCwGG_T
Cuts at: 4995 4995
Size: 10420
SfcI C'TryA_G
Cuts at: 826 1045 3665 4146 5147 6738 7768 8903 9584
Size: 219 2620 481 1001 1591 1030 1135 681
Cuts at: 9584 9775 826
Size: 191 1471
Fragments arranged by size:
2620 1591 1471 1135 1030 1001 681 481
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10420
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10420
SmaI CCC'GGG
Cuts at: 1872 7747 1872
Size: 5875 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2156 2908 4049 5027 5159 5168
Size: 197 926 213 752 1141 978 132 9
Cuts at: 5168 5361 8524 9425 9687 820
Size: 193 3163 901 262 1553
Fragments arranged by size:
3163 1553 1141 978 926 901 752 262
213 197 193 132 9
SnaBI TAC'GTA
Cuts at: 493 8211 493
Size: 7718 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10420
SphI G_CATG'C
Cuts at: 1132 6538 7778 1132
Size: 5406 1240 3774
Fragments arranged by size:
5406 3774 1240
SspI AAT'ATT
Cuts at: 5 52 5810 6214 7057 8344 5
Size: 47 5758 404 843 1287 2081
Fragments arranged by size:
5758 2081 1287 843 404 47
StuI AGG'CCT
Cuts at: 1517 2522 4081 4484 5450 6441 1517
Size: 1005 1559 403 966 991 5496
Fragments arranged by size:
5496 1559 1005 991 966 403
StyI C'CwwG_G
Cuts at: 513 1065 2004 2769 2805 3585 4246 4797 5374
Size: 552 939 765 36 780 661 551 577
Cuts at: 5374 7023 7213 7428 7491 7859 513
Size: 1649 190 215 63 368 3074
Fragments arranged by size:
3074 1649 939 780 765 661 577 552
551 368 215 190 63 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2115 2588 4394 6054 8447 8464 8617
Size: 1255 535 473 1806 1660 2393 17 153
Cuts at: 8617 8802 9247 325
Size: 185 445 1498
Fragments arranged by size:
2393 1806 1660 1498 1255 535 473 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4258 4944 5188
Size: 274 80 33 51 1443 2281 686 244
Cuts at: 5188 6220 6764 8666 10378 96
Size: 1032 544 1902 1712 138
Fragments arranged by size:
2281 1902 1712 1443 1032 686 544 274
244 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 10380 10412 10380
Size: 32 10388
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 10420
XbaI T'CTAG_A
Cuts at: 7756 7756
Size: 10420
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2587 7498 2587
Size: 4911 5509
XhoI C'TCGA_G
Cuts at: 1943 2908 5361 1943
Size: 965 2453 7002
Fragments arranged by size:
7002 2453 965
XmaI C'CCGG_G
Cuts at: 1870 7745 1870
Size: 5875 4545
XmnI GAAnn'nnTTC
Cuts at: 4442 6347 8549 4442
Size: 1905 2202 6313
Fragments arranged by size:
6313 2202 1905
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 8228 276
Size: 53 83 186 936 6694 2468
Fragments arranged by size:
6694 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspOMI PsrI RsrII SacII SfiI
SfoI SrfI SwaI