(Linear) (Six-base) MAPSORT of: KIEE9640.seq Check: 276 from: 1 to: 5715 KIEE9640.orf With 182 enzymes: SgfI * March 22, 2012 18:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 3525 4662 5715 Size: 3525 1137 1053 Fragments arranged by size: 3525 1137 1053 AclI AA'CG_TT Cuts at: 0 4116 5061 5715 Size: 4116 945 654 Fragments arranged by size: 4116 945 654 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 504 1492 2632 3794 4070 4679 4984 5715 Size: 504 988 1140 1162 276 609 305 731 Fragments arranged by size: 1162 1140 988 731 609 504 305 276 AflII C'TTAA_G Cuts at: 0 3022 3163 5715 Size: 3022 141 2552 Fragments arranged by size: 3022 2552 141 AflIII A'CryG_T Cuts at: 0 473 1459 2378 2837 4509 5396 5715 Size: 473 986 919 459 1672 887 319 Fragments arranged by size: 1672 986 919 887 473 459 319 AgeI A'CCGG_T Cuts at: 0 3494 5715 Size: 3494 2221 AhdI GACnn_n'nnGTC Cuts at: 0 4053 4065 5715 Size: 4053 12 1650 Fragments arranged by size: 4053 1650 12 AleI CACnn'nnGTG Cuts at: 0 1531 3535 5715 Size: 1531 2004 2180 Fragments arranged by size: 2180 2004 1531 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2863 2895 5715 Size: 2863 32 2820 Fragments arranged by size: 2863 2820 32 AlwNI CAG_nnn'CTG Cuts at: 0 441 709 1552 4741 5481 5623 5715 Size: 441 268 843 3189 740 142 92 Fragments arranged by size: 3189 843 740 441 268 142 92 ApoI r'AATT_y Cuts at: 0 1327 1789 2509 2955 3637 5317 5609 5715 Size: 1327 462 720 446 682 1680 292 106 Fragments arranged by size: 1680 1327 720 682 462 446 292 106 AvaI C'yCGr_G Cuts at: 0 179 903 1192 1834 2740 2975 3356 4427 Size: 179 724 289 642 906 235 381 1071 Cuts at: 4427 5715 Size: 1288 Fragments arranged by size: 1288 1071 906 724 642 381 289 235 179 BamHI G'GATC_C Cuts at: 0 1170 3906 5715 Size: 1170 2736 1809 Fragments arranged by size: 2736 1809 1170 BanI G'GyrC_C Cuts at: 0 741 3935 5676 5715 Size: 741 3194 1741 39 Fragments arranged by size: 3194 1741 741 39 BanII G_rGCy'C Cuts at: 0 19 2205 2373 2569 2917 3459 3592 3814 Size: 19 2186 168 196 348 542 133 222 Cuts at: 3814 4883 5715 Size: 1069 832 Fragments arranged by size: 2186 1069 832 542 348 222 196 168 133 19 BbsI GAAGACnn'nnnn_ Cuts at: 0 452 467 2259 2456 3782 4109 4958 5155 Size: 452 15 1792 197 1326 327 849 197 Cuts at: 5155 5411 5715 Size: 256 304 Fragments arranged by size: 1792 1326 849 452 327 304 256 197 197 15 BbvCI CC'TCA_GC Cuts at: 0 4544 5447 5715 Size: 4544 903 268 Fragments arranged by size: 4544 903 268 BclI T'GATC_A Cuts at: 0 1901 2540 5715 Size: 1901 639 3175 Fragments arranged by size: 3175 1901 639 BglI GCCn_nnn'nGGC Cuts at: 0 1112 1599 2033 5715 Size: 1112 487 434 3682 Fragments arranged by size: 3682 1112 487 434 BglII A'GATC_T Cuts at: 0 3960 4929 5159 5715 Size: 3960 969 230 556 Fragments arranged by size: 3960 969 556 230 BlpI GC'TnA_GC Cuts at: 0 434 2911 3329 5715 Size: 434 2477 418 2386 Fragments arranged by size: 2477 2386 434 418 Bme1580I G_kGCm'C Cuts at: 0 2212 3111 5679 5715 Size: 2212 899 2568 36 Fragments arranged by size: 2568 2212 899 36 BmgBI CAC'GTC Cuts at: 0 1890 5715 Size: 1890 3825 BmrI ACTGGGnnnn_n' Cuts at: 0 459 2355 2690 3290 3822 3927 4133 4296 Size: 459 1896 335 600 532 105 206 163 Cuts at: 4296 5611 5715 Size: 1315 104 Fragments arranged by size: 1896 1315 600 532 459 335 206 163 105 104 BmtI G_CTAG'C Cuts at: 0 5194 5715 Size: 5194 521 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2342 5121 5715 Size: 2342 2779 594 Fragments arranged by size: 2779 2342 594 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1529 1687 2063 2357 2644 4545 4598 5715 Size: 1529 158 376 294 287 1901 53 1117 Fragments arranged by size: 1901 1529 1117 376 294 287 158 53 Bpu10I CC'TnA_GC Cuts at: 0 2204 4544 5447 5715 Size: 2204 2340 903 268 Fragments arranged by size: 2340 2204 903 268 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 2029 3139 5715 Size: 136 1893 1110 2576 Fragments arranged by size: 2576 1893 1110 136 BsaI GGTCTCn'nnnn_ Cuts at: 0 1289 4310 4399 5005 5715 Size: 1289 3021 89 606 710 Fragments arranged by size: 3021 1289 710 606 89 BsaAI yAC'GTr Cuts at: 0 474 1460 5715 Size: 474 986 4255 Fragments arranged by size: 4255 986 474 BsaBI GATnn'nnATC Cuts at: 0 1495 1553 1846 5715 Size: 1495 58 293 3869 Fragments arranged by size: 3869 1495 293 58 BsaHI Gr'CG_yC Cuts at: 0 4 5715 Size: 4 5711 BsaWI w'CCGG_w Cuts at: 0 3461 3494 5715 Size: 3461 33 2221 Fragments arranged by size: 3461 2221 33 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 126 156 1552 1582 2341 2371 2863 2893 Size: 126 30 1396 30 759 30 492 30 Cuts at: 2893 4903 4933 5715 Size: 2010 30 782 Fragments arranged by size: 2010 1396 782 759 492 126 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 61 64 274 277 394 397 400 403 Size: 61 3 210 3 117 3 3 3 Cuts at: 403 499 1060 1063 1285 1321 2788 3535 3695 Size: 96 561 3 222 36 1467 747 160 Cuts at: 3695 3698 3880 4172 4837 5715 Size: 3 182 292 665 878 Fragments arranged by size: 1467 878 747 665 561 292 222 210 182 160 117 96 61 36 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 0 222 870 2826 3361 3585 4571 5592 5705 Size: 222 648 1956 535 224 986 1021 113 Cuts at: 5705 5715 Size: 10 Fragments arranged by size: 1956 1021 986 648 535 224 222 113 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1504 1657 2140 4625 5512 5715 Size: 1504 153 483 2485 887 203 Fragments arranged by size: 2485 1504 887 483 203 153 BsiHKAI G_wGCw'C Cuts at: 0 3330 3459 3814 5715 Size: 3330 129 355 1901 Fragments arranged by size: 3330 1901 355 129 Bsp1286I G_dGCh'C Cuts at: 0 19 2205 2212 2373 2569 2917 3111 3330 Size: 19 2186 7 161 196 348 194 219 Cuts at: 3330 3459 3592 3814 4883 5679 5715 Size: 129 133 222 1069 796 36 Fragments arranged by size: 2186 1069 796 348 222 219 196 194 161 133 129 36 19 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 3227 3525 4328 4662 5688 5715 Size: 3227 298 803 334 1026 27 Fragments arranged by size: 3227 1026 803 334 298 27 BsrBI CCG'CTC Cuts at: 0 1599 2352 5127 5715 Size: 1599 753 2775 588 Fragments arranged by size: 2775 1599 753 588 BsrDI GCAATG_nn' Cuts at: 0 542 2786 3071 4174 5075 5600 5715 Size: 542 2244 285 1103 901 525 115 Fragments arranged by size: 2244 1103 901 542 525 285 115 BsrFI r'CCGG_y Cuts at: 0 3494 5715 Size: 3494 2221 BsrGI T'GTAC_A Cuts at: 0 2253 4759 5715 Size: 2253 2506 956 Fragments arranged by size: 2506 2253 956 BssSI C'ACGA_G Cuts at: 0 812 5715 Size: 812 4903 BstAPI GCAn_nnn'nTGC Cuts at: 0 2823 2841 2971 3150 3325 5715 Size: 2823 18 130 179 175 2390 Fragments arranged by size: 2823 2390 179 175 130 18 BstEII G'GTnAC_C Cuts at: 0 4580 5715 Size: 4580 1135 BstXI CCAn_nnnn'nTGG Cuts at: 0 2553 5715 Size: 2553 3162 BstYI r'GATC_y Cuts at: 0 898 1170 1274 1424 1490 1958 3018 3601 Size: 898 272 104 150 66 468 1060 583 Cuts at: 3601 3906 3960 4929 5159 5715 Size: 305 54 969 230 556 Fragments arranged by size: 1060 969 898 583 556 468 305 272 230 150 104 66 54 Bsu36I CC'TnA_GG Cuts at: 0 4283 4814 5715 Size: 4283 531 901 Fragments arranged by size: 4283 901 531 BtgI C'CryG_G Cuts at: 0 5208 5289 5423 5715 Size: 5208 81 134 292 Fragments arranged by size: 5208 292 134 81 BtsI GCAGTG_nn' Cuts at: 0 48 1215 1398 1638 3111 3386 3717 5715 Size: 48 1167 183 240 1473 275 331 1998 Fragments arranged by size: 1998 1473 1167 331 275 240 183 48 ClaI AT'CG_AT Cuts at: 0 3266 5715 Size: 3266 2449 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 696 731 1332 1367 5715 Size: 696 35 601 35 4348 Fragments arranged by size: 4348 696 601 35 35 DraIII CAC_nnn'GTG Cuts at: 0 1872 3325 5715 Size: 1872 1453 2390 Fragments arranged by size: 2390 1872 1453 DrdI GACnn_nn'nnGTC Cuts at: 0 2383 3510 5715 Size: 2383 1127 2205 Fragments arranged by size: 2383 2205 1127 EaeI y'GGCC_r Cuts at: 0 1591 3383 5715 Size: 1591 1792 2332 Fragments arranged by size: 2332 1792 1591 EarI CTCTTCn'nnn_ Cuts at: 0 101 113 263 352 1277 1298 1748 2702 Size: 101 12 150 89 925 21 450 954 Cuts at: 2702 3055 3857 4694 4955 4973 5117 5715 Size: 353 802 837 261 18 144 598 Fragments arranged by size: 954 925 837 802 598 450 353 261 150 144 101 89 21 18 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 5479 5715 Size: 5479 236 EcoICRI GAG'CTC Cuts at: 0 3457 3812 5715 Size: 3457 355 1903 Fragments arranged by size: 3457 1903 355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 504 1492 1529 1687 2063 2357 2632 2644 Size: 504 988 37 158 376 294 275 12 Cuts at: 2644 3794 4070 4545 4598 4679 4984 5715 Size: 1150 276 475 53 81 305 731 Fragments arranged by size: 1150 988 731 504 475 376 305 294 276 275 158 81 53 37 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 1282 1303 2284 3166 3223 5485 5715 Size: 1282 21 981 882 57 2262 230 Fragments arranged by size: 2262 1282 981 882 230 57 21 EcoO109I rG'GnC_Cy Cuts at: 0 111 183 778 1782 2175 2305 3366 4149 Size: 111 72 595 1004 393 130 1061 783 Cuts at: 4149 4767 5022 5715 Size: 618 255 693 Fragments arranged by size: 1061 1004 783 693 618 595 393 255 130 111 72 EcoRI G'AATT_C Cuts at: 0 1327 5609 5715 Size: 1327 4282 106 Fragments arranged by size: 4282 1327 106 EcoRV GAT'ATC Cuts at: 0 3630 5715 Size: 3630 2085 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 4267 5715 Size: 4267 1448 HaeII r_GCGC'y Cuts at: 0 210 2324 4524 5715 Size: 210 2114 2200 1191 Fragments arranged by size: 2200 2114 1191 210 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 112 144 409 441 916 948 4039 4051 Size: 112 32 265 32 475 32 3091 12 Cuts at: 4051 4071 4083 4714 4746 4903 4935 5151 5183 Size: 20 12 631 32 157 32 216 32 Cuts at: 5183 5715 Size: 532 Fragments arranged by size: 3091 631 532 475 265 216 157 112 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 0 4008 5715 Size: 4008 1707 HindIII A'AGCT_T Cuts at: 0 334 2056 5715 Size: 334 1722 3659 Fragments arranged by size: 3659 1722 334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2048 2125 4082 5715 Size: 2048 77 1957 1633 Fragments arranged by size: 2048 1957 1633 77 MscI TGG'CCA Cuts at: 0 3385 5715 Size: 3385 2330 MslI CAynn'nnrTG Cuts at: 0 1531 2551 3535 3725 3941 4379 4514 5213 Size: 1531 1020 984 190 216 438 135 699 Cuts at: 5213 5715 Size: 502 Fragments arranged by size: 1531 1020 984 699 502 438 216 190 135 MspA1I CmG'CkG Cuts at: 0 661 676 1665 1704 5373 5547 5715 Size: 661 15 989 39 3669 174 168 Fragments arranged by size: 3669 989 661 174 168 39 15 NcoI C'CATG_G Cuts at: 0 5208 5423 5715 Size: 5208 215 292 Fragments arranged by size: 5208 292 215 NdeI CA'TA_TG Cuts at: 0 1322 4353 5715 Size: 1322 3031 1362 Fragments arranged by size: 3031 1362 1322 NheI G'CTAG_C Cuts at: 0 5190 5715 Size: 5190 525 NspI r_CATG'y Cuts at: 0 1629 2382 2826 2841 2877 3096 3291 4453 Size: 1629 753 444 15 36 219 195 1162 Cuts at: 4453 4513 4533 5400 5715 Size: 60 20 867 315 Fragments arranged by size: 1629 1162 867 753 444 315 219 195 60 36 20 15 PciI A'CATG_T Cuts at: 0 2378 2837 4509 5396 5715 Size: 2378 459 1672 887 319 Fragments arranged by size: 2378 1672 887 459 319 PflMI CCAn_nnn'nTGG Cuts at: 0 1323 1732 1872 2757 3834 5715 Size: 1323 409 140 885 1077 1881 Fragments arranged by size: 1881 1323 1077 885 409 140 PfoI T'CCnGG_A Cuts at: 0 440 1277 1961 5715 Size: 440 837 684 3754 Fragments arranged by size: 3754 837 684 440 PmlI CAC'GTG Cuts at: 0 474 5715 Size: 474 5241 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1588 1620 2422 2454 2863 2895 4327 4359 Size: 1588 32 802 32 409 32 1432 32 Cuts at: 4359 5715 Size: 1356 Fragments arranged by size: 1588 1432 1356 802 409 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 0 111 183 778 1782 5022 5715 Size: 111 72 595 1004 3240 693 Fragments arranged by size: 3240 1004 693 595 111 72 PshAI GACnn'nnGTC Cuts at: 0 2263 5715 Size: 2263 3452 PsiI TTA'TAA Cuts at: 0 3251 5203 5715 Size: 3251 1952 512 Fragments arranged by size: 3251 1952 512 PspXI vC'TCGA_Gb Cuts at: 0 903 3356 5715 Size: 903 2453 2359 Fragments arranged by size: 2453 2359 903 PstI C_TGCA'G Cuts at: 0 1664 3146 5715 Size: 1664 1482 2569 Fragments arranged by size: 2569 1664 1482 PvuII CAG'CTG Cuts at: 0 661 1665 5373 5547 5715 Size: 661 1004 3708 174 168 Fragments arranged by size: 3708 1004 661 174 168 SacI G_AGCT'C Cuts at: 0 3459 3814 5715 Size: 3459 355 1901 Fragments arranged by size: 3459 1901 355 SanDI GG'GwC_CC Cuts at: 0 183 5715 Size: 183 5532 SapI GCTCTTCn'nnn_ Cuts at: 0 352 5117 5715 Size: 352 4765 598 Fragments arranged by size: 4765 598 352 ScaI AGT'ACT Cuts at: 0 4217 5715 Size: 4217 1498 SexAI A'CCwGG_T Cuts at: 0 2990 5715 Size: 2990 2725 SfcI C'TryA_G Cuts at: 0 1660 2141 3142 4733 5715 Size: 1660 481 1001 1591 982 Fragments arranged by size: 1660 1591 1001 982 481 SmlI C'TyrA_G Cuts at: 0 151 903 2044 3022 3154 3163 3356 5715 Size: 151 752 1141 978 132 9 193 2359 Fragments arranged by size: 2359 1141 978 752 193 151 132 9 SphI G_CATG'C Cuts at: 0 4533 5715 Size: 4533 1182 SspI AAT'ATT Cuts at: 0 3805 4209 5052 5715 Size: 3805 404 843 663 Fragments arranged by size: 3805 843 663 404 StuI AGG'CCT Cuts at: 0 517 2076 2479 3445 4436 5715 Size: 517 1559 403 966 991 1279 Fragments arranged by size: 1559 1279 991 966 517 403 StyI C'CwwG_G Cuts at: 0 764 800 1580 2241 2792 3369 5018 5208 Size: 764 36 780 661 551 577 1649 190 Cuts at: 5208 5423 5486 5715 Size: 215 63 229 Fragments arranged by size: 1649 780 764 661 577 551 229 215 190 63 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 110 583 2389 4049 5715 Size: 110 473 1806 1660 1666 Fragments arranged by size: 1806 1666 1660 473 110 TatI w'GTAC_w Cuts at: 0 2253 2939 3183 4215 4759 5715 Size: 2253 686 244 1032 544 956 Fragments arranged by size: 2253 1032 956 686 544 244 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 582 5493 5715 Size: 582 4911 222 Fragments arranged by size: 4911 582 222 XhoI C'TCGA_G Cuts at: 0 903 3356 5715 Size: 903 2453 2359 Fragments arranged by size: 2453 2359 903 XmnI GAAnn'nnTTC Cuts at: 0 2437 4342 5715 Size: 2437 1905 1373 Fragments arranged by size: 2437 1905 1373 Enzymes that do cut: AarI AclI AcuI AflII AflIII AgeI AhdI AleI AloI AlwNI ApoI AvaI BamHI BanI BanII BbsI BbvCI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI ClaI CspCI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NdeI NheI NspI PciI PflMI PfoI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuII SacI SanDI SapI ScaI SexAI SfcI SmlI SphI SspI StuI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AatII AccI Acc65I AfeI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI BstZ17I BtgZI DraI EagI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NgoMIV NotI NruI NsiI PacI PasI PmeI PspOMI PsrI PvuI RsrII SacII SalI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI Tth111I XbaI XmaI ZraI