Restriction map for
pFN21AE3760
(Circular) (Six-base) MAPSORT of: pFN21AE3760.seq Check: 6682 from: 1 to: 6187
pFN21AE3760 6187 bp
With 182 enzymes: SgfI *
March 28, 2012 12:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2421 2421
Size: 6187
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 2040 3530 1937
Size: 72 31 1490 4594
Fragments arranged by size:
4594 1490 72 31
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3274 3445 4250 5634 5902 1632
Size: 1642 171 805 1384 268 1917
Fragments arranged by size:
1917 1642 1384 805 268 171
AflII C'TTAA_G
Cuts at: 820 1017 2306 820
Size: 197 1289 4701
Fragments arranged by size:
4701 1289 197
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 3361 4916 3361
Size: 1555 4632
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2354 1184
Size: 1170 5017
AlwNI CAG_nnn'CTG
Cuts at: 2262 3211 3930 5502 2262
Size: 949 719 1572 2947
Fragments arranged by size:
2947 1572 949 719
ApaI G_GGCC'C
Cuts at: 3049 3049
Size: 6187
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2357 3496 3722 3874 5983 1493
Size: 864 1139 226 152 2109 1697
Fragments arranged by size:
2109 1697 1139 864 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 2140 2239 2785 2815 2825 2950 3512
Size: 73 197 99 546 30 10 125 562
Cuts at: 3512 1870
Size: 4545
Fragments arranged by size:
4545 562 546 197 125 99 73 30
10
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2243 2300 2687 2954 3011
Size: 325 217 627 456 57 387 267 57
Cuts at: 3011 3020 3037 3214 3235 3508 4963 618
Size: 9 17 177 21 273 1455 1842
Fragments arranged by size:
1842 1455 627 456 387 325 273 267
217 177 57 57 21 17 9
BanII G_rGCy'C
Cuts at: 1391 3049 3111 3401 3506 1391
Size: 1658 62 290 105 4072
Fragments arranged by size:
4072 1658 290 105 62
BbeI G_GCGC'C
Cuts at: 2247 2691 2958 3024 3041 3218 3239 2247
Size: 444 267 66 17 177 21 5195
Fragments arranged by size:
5195 444 267 177 66 21 17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5216
BbvCI CC'TCA_GC
Cuts at: 2777 3410 2777
Size: 633 5554
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3261 3295 4378 4412 1903
Size: 34 1324 34 1083 34 3678
Fragments arranged by size:
3678 1324 1083 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2936 2978 3161
Size: 107 122 71 631 605 1264 42 183
Cuts at: 3161 3414 4798 136
Size: 253 1384 1525
Fragments arranged by size:
1525 1384 1264 631 605 253 183 122
107 71 42
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3110 3604 3110
Size: 494 5693
Bme1580I G_kGCm'C
Cuts at: 1790 3049 4248 5404 1790
Size: 1259 1199 1156 2573
Fragments arranged by size:
2573 1259 1199 1156
BmgBI CAC'GTC
Cuts at: 1318 2856 1318
Size: 1538 4649
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4847 1239
Size: 3608 2579
Bpu10I CC'TnA_GC
Cuts at: 2777 3383 3395 3410 2777
Size: 606 12 15 5554
Fragments arranged by size:
5554 606 15 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4312 5177 5475 4312
Size: 865 298 5024
Fragments arranged by size:
5024 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2505 3978 493
Size: 711 1301 1473 2702
Fragments arranged by size:
2702 1473 1301 711
BsaBI GATnn'nnATC
Cuts at: 6181 6181
Size: 6187
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2117 2244 2688 2955
Size: 53 83 186 936 584 127 444 267
Cuts at: 2955 3021 3038 3215 3236 3994 4376 5011 275
Size: 66 17 177 21 758 382 635 1451
Fragments arranged by size:
1451 936 758 635 584 444 382 267
186 177 127 83 66 53 21 17
BsaWI w'CCGG_w
Cuts at: 3103 4620 5018 5292 5439 5850 3103
Size: 1517 398 274 147 411 3440
Fragments arranged by size:
3440 1517 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5962
Fragments arranged by size:
5962 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2507 2893 2896 2905 1186
Size: 1321 386 3 9 4468
Fragments arranged by size:
4468 1321 386 9 3
BseYI C'CCAG_C
Cuts at: 2135 2210 3242 3344 3428 5390 5886 2135
Size: 75 1032 102 84 1962 496 2436
Fragments arranged by size:
2436 1962 1032 496 102 84 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 2750 1163
Size: 573 657 357 4600
Fragments arranged by size:
4600 657 573 357
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2280 2392 2985 2997 3637 4398
Size: 275 186 279 112 593 12 640 761
Cuts at: 4398 5426 1540
Size: 1028 2301
Fragments arranged by size:
2301 1028 761 640 593 279 275 186
112 12
BsiHKAI G_wGCw'C
Cuts at: 3111 3401 4248 4333 5404 3111
Size: 290 847 85 1071 3894
Fragments arranged by size:
3894 1071 847 290 85
BsmI GAATG_Cn'
Cuts at: 3698 3791 3698
Size: 93 6094
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3049 3111 3401 3506 4248 4333 5404
Size: 399 1259 62 290 105 742 85 1071
Cuts at: 5404 1391
Size: 2174
Fragments arranged by size:
2174 1259 1071 742 399 290 105 85
62
BspEI T'CCGG_A
Cuts at: 3103 5850 3103
Size: 2747 3440
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 2421 2709 3039 3542 844
Size: 1349 228 288 330 503 3489
Fragments arranged by size:
3489 1349 503 330 288 228
BsrBI CCG'CTC
Cuts at: 1394 1823 2370 2499 2665 2999 3130 4074 1394
Size: 429 547 129 166 334 131 944 3507
Fragments arranged by size:
3507 944 547 429 334 166 131 129
BsrDI GCAATG_nn'
Cuts at: 65 1248 4682 4856 65
Size: 1183 3434 174 1396
Fragments arranged by size:
3434 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2279 2481 3232 4831 5018 1700
Size: 110 469 202 751 1599 187 2869
Fragments arranged by size:
2869 1599 751 469 202 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6187
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 2546 3073 4247 5259 2546
Size: 527 1174 1012 3474
Fragments arranged by size:
3474 1174 1012 527
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2262 2407 794
Size: 1468 145 4574
Fragments arranged by size:
4574 1468 145
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 3517 3884 4269 4286 5738 5824 5836 6182
Size: 1545 367 385 17 1452 86 12 346
Cuts at: 6182 1972
Size: 1977
Fragments arranged by size:
1977 1545 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2744 2753 2934 2976 2985 3159 513
Size: 552 1679 9 181 42 9 174 3541
Fragments arranged by size:
3541 1679 552 181 174 42 9 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2736 487
Size: 683 56 1510 3938
Fragments arranged by size:
3938 1510 683 56
BtsI GCAGTG_nn'
Cuts at: 3703 4497 4517 3703
Size: 794 20 5373
Fragments arranged by size:
5373 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5194 809
Size: 507 3878 1802
Fragments arranged by size:
3878 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2070 2124 2175
Size: 54 1425 50 275 18 240 54 51
Cuts at: 2175 2277 2982 2994 3162 3442 3634 4523 6152
Size: 102 705 12 168 280 192 889 1629
Cuts at: 6152 8
Size: 43
Fragments arranged by size:
1629 1425 889 705 280 275 240 192
168 102 54 54 51 50 43 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 2277 2982 2994 3634 1537
Size: 275 465 705 12 640 4090
Fragments arranged by size:
4090 705 640 465 275 12
EarI CTCTTCn'nnn_
Cuts at: 1353 2890 4117 1353
Size: 1537 1227 3423
Fragments arranged by size:
3423 1537 1227
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2014 2899 4774 5148 5294 387
Size: 1064 138 425 885 1875 374 146 1280
Fragments arranged by size:
1875 1280 1064 885 425 374 146 138
EcoICRI GAG'CTC
Cuts at: 3109 3399 3109
Size: 290 5897
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3274 3445 4250 4847 5634 5902 1239
Size: 393 1642 171 805 597 787 268 1524
Fragments arranged by size:
1642 1524 805 787 597 393 268 171
EcoO109I rG'GnC_Cy
Cuts at: 2698 3028 3045 3046 3208 2698
Size: 330 17 1 162 5677
Fragments arranged by size:
5677 330 162 17 1
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FspI TGC'GCA
Cuts at: 2555 4693 2555
Size: 2138 4049
HaeII r_GCGC'y
Cuts at: 1678 2247 2691 2958 3024 3041 3218 3239 5334
Size: 569 444 267 66 17 177 21 2095
Cuts at: 5334 1678
Size: 2531
Fragments arranged by size:
2531 2095 569 444 267 177 66 21
17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2554 2586 3347 3379 4828
Size: 32 135 32 982 32 761 32 1449
Cuts at: 4828 4860 4902 4934 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1449 982 761 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2010 2041 3531 3777 669
Size: 988 281 72 31 1490 246 3079
Fragments arranged by size:
3079 1490 988 281 246 72 31
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6187
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KasI G'GCGC_C
Cuts at: 2243 2687 2954 3020 3037 3214 3235 2243
Size: 444 267 66 17 177 21 5195
Fragments arranged by size:
5195 444 267 177 66 21 17
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2188 2312 3342 3345 3504 5301 5485 1305
Size: 883 124 1030 3 159 1797 184 2007
Fragments arranged by size:
2007 1797 1030 883 184 159 124 3
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2072 10
Size: 54 1425 343 240 4125
Fragments arranged by size:
4125 1425 343 240 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4145 4504 4663 518
Size: 648 2979 359 159 2042
Fragments arranged by size:
2979 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2223 2262 2746 2755 2836 2936 2978 2987
Size: 617 39 484 9 81 100 42 9
Cuts at: 2987 3301 3414 3603 4280 5428 5673 1606
Size: 314 113 189 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 617 484 314 245 189
113 100 81 42 39 9 9
NaeI GCC'GGC
Cuts at: 2281 2483 3234 2281
Size: 202 751 5234
Fragments arranged by size:
5234 751 202
NarI GG'CG_CC
Cuts at: 2244 2688 2955 3021 3038 3215 3236 2244
Size: 444 267 66 17 177 21 5195
Fragments arranged by size:
5195 444 267 177 66 21 17
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5635
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6187
NgoMIV G'CCGG_C
Cuts at: 2279 2481 3232 2279
Size: 202 751 5234
Fragments arranged by size:
5234 751 202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6187
NotI GC'GGCC_GC
Cuts at: 2982 2994 3634 2982
Size: 12 640 5535
Fragments arranged by size:
5535 640 12
NspI r_CATG'y
Cuts at: 1132 1229 2095 2192 2967 3545 5090 1132
Size: 97 866 97 775 578 1545 2229
Fragments arranged by size:
2229 1545 866 775 578 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6187
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2361 2361
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PmlI CAC'GTG
Cuts at: 2505 2505
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4256 4288 5769 5801 4256
Size: 32 1481 32 4642
Fragments arranged by size:
4642 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2698 3028 2698
Size: 330 5857
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 3757 3757
Size: 6187
PspOMI G'GGCC_C
Cuts at: 3045 3045
Size: 6187
PspXI vC'TCGA_Gb
Cuts at: 2825 2825
Size: 6187
PstI C_TGCA'G
Cuts at: 830 2418 3539 830
Size: 1588 1121 3478
Fragments arranged by size:
3478 1588 1121
PvuI CG_AT'CG
Cuts at: 2001 2392 2001
Size: 391 5796
PvuII CAG'CTG
Cuts at: 2223 2262 3301 2223
Size: 39 1039 5109
Fragments arranged by size:
5109 1039 39
RsrII CG'GwC_CG
Cuts at: 2285 3101 3367 2285
Size: 816 266 5105
Fragments arranged by size:
5105 816 266
SacI G_AGCT'C
Cuts at: 3111 3401 3111
Size: 290 5897
SacII CC_GC'GG
Cuts at: 2747 2756 2937 2979 2988 2747
Size: 9 181 42 9 5946
Fragments arranged by size:
5946 181 42 9 9
SalI G'TCGA_C
Cuts at: 1936 2008 2039 3529 1936
Size: 72 31 1490 4594
Fragments arranged by size:
4594 1490 72 31
SapI GCTCTTCn'nnn_
Cuts at: 2890 2890
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2414 3535 4670 5351 5542 826
Size: 219 1369 1121 1135 681 191 1471
Fragments arranged by size:
1471 1369 1135 1121 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2936 2936
Size: 6187
SfoI GGC'GCC
Cuts at: 2245 2689 2956 3022 3039 3216 3237 2245
Size: 444 267 66 17 177 21 5195
Fragments arranged by size:
5195 444 267 177 66 21 17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 3232 1700
Size: 1532 4655
SmaI CCC'GGG
Cuts at: 1872 2241 2817 3514 1872
Size: 369 576 697 4545
Fragments arranged by size:
4545 697 576 369
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 2825 4291 5192 5454 820
Size: 197 926 363 519 1466 901 262 1553
Fragments arranged by size:
1553 1466 926 901 519 363 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 2192 3545 1132
Size: 1060 1353 3774
Fragments arranged by size:
3774 1353 1060
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6187
StyI C'CwwG_G
Cuts at: 513 1065 3626 513
Size: 552 2561 3074
Fragments arranged by size:
3074 2561 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2486 2600 3089 4214 4231 4384 4569
Size: 1255 906 114 489 1125 17 153 185
Cuts at: 4569 5014 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1125 906 489 445 185 153
114 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2577 4433 6145
Size: 274 80 33 51 1443 600 1856 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1856 1712 1443 600 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2534 2534
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 2825 1943
Size: 882 5305
XmaI C'CCGG_G
Cuts at: 1870 2239 2815 3512 1870
Size: 369 576 697 4545
Fragments arranged by size:
4545 697 576 369
XmnI GAAnn'nnTTC
Cuts at: 2357 4316 2357
Size: 1959 4228
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PsrI SanDI SexAI
SrfI SwaI