(Linear) (Six-base) MAPSORT of: KIEE3760.seq Check: 4415 from: 1 to: 1482 KIEE3760.orf With 182 enzymes: SgfI * March 22, 2012 16:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 416 1482 Size: 416 1066 AccI GT'mk_AC Cuts at: 0 4 35 1482 Size: 4 31 1447 Fragments arranged by size: 1447 31 4 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1269 1440 1482 Size: 1269 171 42 Fragments arranged by size: 1269 171 42 AflII C'TTAA_G Cuts at: 0 301 1482 Size: 301 1181 AhdI GACnn_n'nnGTC Cuts at: 0 1356 1482 Size: 1356 126 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 349 1482 Size: 349 1133 AlwNI CAG_nnn'CTG Cuts at: 0 257 1206 1482 Size: 257 949 276 Fragments arranged by size: 949 276 257 ApaI G_GGCC'C Cuts at: 0 1044 1482 Size: 1044 438 ApoI r'AATT_y Cuts at: 0 352 1482 Size: 352 1130 AvaI C'yCGr_G Cuts at: 0 135 234 780 810 820 945 1482 Size: 135 99 546 30 10 125 537 Fragments arranged by size: 546 537 135 125 99 30 10 BanI G'GyrC_C Cuts at: 0 238 295 682 949 1006 1015 1032 1209 Size: 238 57 387 267 57 9 17 177 Cuts at: 1209 1230 1482 Size: 21 252 Fragments arranged by size: 387 267 252 238 177 57 57 21 17 9 BanII G_rGCy'C Cuts at: 0 1044 1106 1396 1482 Size: 1044 62 290 86 Fragments arranged by size: 1044 290 86 62 BbeI G_GCGC'C Cuts at: 0 242 686 953 1019 1036 1213 1234 1482 Size: 242 444 267 66 17 177 21 248 Fragments arranged by size: 444 267 248 242 177 66 21 17 BbvCI CC'TCA_GC Cuts at: 0 772 1405 1482 Size: 772 633 77 Fragments arranged by size: 772 633 77 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1256 1290 1482 Size: 1256 34 192 Fragments arranged by size: 1256 192 34 BglI GCCn_nnn'nGGC Cuts at: 0 931 973 1156 1409 1482 Size: 931 42 183 253 73 Fragments arranged by size: 931 253 183 73 42 BlpI GC'TnA_GC Cuts at: 0 1105 1482 Size: 1105 377 Bme1580I G_kGCm'C Cuts at: 0 1044 1482 Size: 1044 438 BmgBI CAC'GTC Cuts at: 0 851 1482 Size: 851 631 Bpu10I CC'TnA_GC Cuts at: 0 772 1378 1390 1405 1482 Size: 772 606 12 15 77 Fragments arranged by size: 772 606 77 15 12 BsaAI yAC'GTr Cuts at: 0 500 1482 Size: 500 982 BsaHI Gr'CG_yC Cuts at: 0 112 239 683 950 1016 1033 1210 1231 Size: 112 127 444 267 66 17 177 21 Cuts at: 1231 1482 Size: 251 Fragments arranged by size: 444 267 251 177 127 112 66 21 17 BsaWI w'CCGG_w Cuts at: 0 1098 1482 Size: 1098 384 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 502 888 891 900 1482 Size: 502 386 3 9 582 Fragments arranged by size: 582 502 386 9 3 BseYI C'CCAG_C Cuts at: 0 130 205 1237 1339 1423 1482 Size: 130 75 1032 102 84 59 Fragments arranged by size: 1032 130 102 84 75 59 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 388 745 1482 Size: 388 357 737 Fragments arranged by size: 737 388 357 BsiEI CG_ry'CG Cuts at: 0 275 387 980 992 1482 Size: 275 112 593 12 490 Fragments arranged by size: 593 490 275 112 12 BsiHKAI G_wGCw'C Cuts at: 0 1106 1396 1482 Size: 1106 290 86 Fragments arranged by size: 1106 290 86 Bsp1286I G_dGCh'C Cuts at: 0 1044 1106 1396 1482 Size: 1044 62 290 86 Fragments arranged by size: 1044 290 86 62 BspEI T'CCGG_A Cuts at: 0 1098 1482 Size: 1098 384 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 188 416 704 1034 1482 Size: 188 228 288 330 448 Fragments arranged by size: 448 330 288 228 188 BsrBI CCG'CTC Cuts at: 0 365 494 660 994 1125 1482 Size: 365 129 166 334 131 357 Fragments arranged by size: 365 357 334 166 131 129 BsrFI r'CCGG_y Cuts at: 0 274 476 1227 1482 Size: 274 202 751 255 Fragments arranged by size: 751 274 255 202 BssSI C'ACGA_G Cuts at: 0 541 1068 1482 Size: 541 527 414 Fragments arranged by size: 541 527 414 BstAPI GCAn_nnn'nTGC Cuts at: 0 257 402 1482 Size: 257 145 1080 Fragments arranged by size: 1080 257 145 BtgI C'CryG_G Cuts at: 0 739 748 929 971 980 1154 1482 Size: 739 9 181 42 9 174 328 Fragments arranged by size: 739 328 181 174 42 9 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 731 1482 Size: 731 751 EaeI y'GGCC_r Cuts at: 0 65 119 170 272 977 989 1157 1437 Size: 65 54 51 102 705 12 168 280 Cuts at: 1437 1482 Size: 45 Fragments arranged by size: 705 280 168 102 65 54 51 45 12 EagI C'GGCC_G Cuts at: 0 272 977 989 1482 Size: 272 705 12 493 Fragments arranged by size: 705 493 272 12 EarI CTCTTCn'nnn_ Cuts at: 0 885 1482 Size: 885 597 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 9 894 1482 Size: 9 885 588 Fragments arranged by size: 885 588 9 EcoICRI GAG'CTC Cuts at: 0 1104 1394 1482 Size: 1104 290 88 Fragments arranged by size: 1104 290 88 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1269 1440 1482 Size: 1269 171 42 Fragments arranged by size: 1269 171 42 EcoO109I rG'GnC_Cy Cuts at: 0 693 1023 1040 1041 1203 1482 Size: 693 330 17 1 162 279 Fragments arranged by size: 693 330 279 162 17 1 FspI TGC'GCA Cuts at: 0 550 1482 Size: 550 932 HaeII r_GCGC'y Cuts at: 0 242 686 953 1019 1036 1213 1234 1482 Size: 242 444 267 66 17 177 21 248 Fragments arranged by size: 444 267 248 242 177 66 21 17 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 549 581 1342 1374 1482 Size: 549 32 761 32 108 Fragments arranged by size: 761 549 108 32 32 HincII GTy'rAC Cuts at: 0 5 36 1482 Size: 5 31 1446 Fragments arranged by size: 1446 31 5 KasI G'GCGC_C Cuts at: 0 238 682 949 1015 1032 1209 1230 1482 Size: 238 444 267 66 17 177 21 252 Fragments arranged by size: 444 267 252 238 177 66 21 17 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 183 307 1337 1340 1482 Size: 183 124 1030 3 142 Fragments arranged by size: 1030 183 142 124 3 MscI TGG'CCA Cuts at: 0 67 1482 Size: 67 1415 MspA1I CmG'CkG Cuts at: 0 218 257 741 750 831 931 973 982 Size: 218 39 484 9 81 100 42 9 Cuts at: 982 1296 1409 1482 Size: 314 113 73 Fragments arranged by size: 484 314 218 113 100 81 73 42 39 9 9 NaeI GCC'GGC Cuts at: 0 276 478 1229 1482 Size: 276 202 751 253 Fragments arranged by size: 751 276 253 202 NarI GG'CG_CC Cuts at: 0 239 683 950 1016 1033 1210 1231 1482 Size: 239 444 267 66 17 177 21 251 Fragments arranged by size: 444 267 251 239 177 66 21 17 NgoMIV G'CCGG_C Cuts at: 0 274 476 1227 1482 Size: 274 202 751 255 Fragments arranged by size: 751 274 255 202 NotI GC'GGCC_GC Cuts at: 0 977 989 1482 Size: 977 12 493 Fragments arranged by size: 977 493 12 NspI r_CATG'y Cuts at: 0 90 187 962 1482 Size: 90 97 775 520 Fragments arranged by size: 775 520 97 90 PfoI T'CCnGG_A Cuts at: 0 356 1482 Size: 356 1126 PmlI CAC'GTG Cuts at: 0 500 1482 Size: 500 982 PpuMI rG'GwC_Cy Cuts at: 0 693 1023 1482 Size: 693 330 459 Fragments arranged by size: 693 459 330 PspOMI G'GGCC_C Cuts at: 0 1040 1482 Size: 1040 442 PspXI vC'TCGA_Gb Cuts at: 0 820 1482 Size: 820 662 PstI C_TGCA'G Cuts at: 0 413 1482 Size: 413 1069 PvuI CG_AT'CG Cuts at: 0 387 1482 Size: 387 1095 PvuII CAG'CTG Cuts at: 0 218 257 1296 1482 Size: 218 39 1039 186 Fragments arranged by size: 1039 218 186 39 RsrII CG'GwC_CG Cuts at: 0 280 1096 1362 1482 Size: 280 816 266 120 Fragments arranged by size: 816 280 266 120 SacI G_AGCT'C Cuts at: 0 1106 1396 1482 Size: 1106 290 86 Fragments arranged by size: 1106 290 86 SacII CC_GC'GG Cuts at: 0 742 751 932 974 983 1482 Size: 742 9 181 42 9 499 Fragments arranged by size: 742 499 181 42 9 9 SalI G'TCGA_C Cuts at: 0 3 34 1482 Size: 3 31 1448 Fragments arranged by size: 1448 31 3 SapI GCTCTTCn'nnn_ Cuts at: 0 885 1482 Size: 885 597 SfcI C'TryA_G Cuts at: 0 409 1482 Size: 409 1073 SfiI GGCCn_nnn'nGGCC Cuts at: 0 931 1482 Size: 931 551 SfoI GGC'GCC Cuts at: 0 240 684 951 1017 1034 1211 1232 1482 Size: 240 444 267 66 17 177 21 250 Fragments arranged by size: 444 267 250 240 177 66 21 17 SgrAI Cr'CCGG_yG Cuts at: 0 1227 1482 Size: 1227 255 SmaI CCC'GGG Cuts at: 0 236 812 1482 Size: 236 576 670 Fragments arranged by size: 670 576 236 SmlI C'TyrA_G Cuts at: 0 301 820 1482 Size: 301 519 662 Fragments arranged by size: 662 519 301 SphI G_CATG'C Cuts at: 0 187 1482 Size: 187 1295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 481 595 1084 1482 Size: 481 114 489 398 Fragments arranged by size: 489 481 398 114 TatI w'GTAC_w Cuts at: 0 572 1482 Size: 572 910 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 529 1482 Size: 529 953 XhoI C'TCGA_G Cuts at: 0 820 1482 Size: 820 662 XmaI C'CCGG_G Cuts at: 0 234 810 1482 Size: 234 576 672 Fragments arranged by size: 672 576 234 XmnI GAAnn'nnTTC Cuts at: 0 352 1482 Size: 352 1130 Enzymes that do cut: AarI AccI AcuI AflII AhdI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbeI BbvCI BcgI BglI BlpI Bme1580I BmgBI Bpu10I BsaAI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BtgI BtgZI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI MspA1I NaeI NarI NgoMIV NotI NspI PfoI PmlI PpuMI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SapI SfcI SfiI SfoI SgrAI SmaI SmlI SphI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflIII AgeI AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI BclI BfrBI BglII BmrI BmtI BplI BpmI BpuEI BsaI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EcoNI EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MslI NcoI NdeI NheI NruI NsiI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI SanDI SbfI ScaI SexAI SgfI SnaBI SpeI SrfI SspI StuI StyI SwaI TstI Tth111I XbaI ZraI