################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Alw44I ApaLI AscI Asp700I Asp718I AspEI AviII AvrII BclI BfaI BfrI BlnI BsiWI BsmI Bsp1407I BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraIII Eam1105I EcoO65I EcoRI EcoRV EcoT22I FbaI FspI HincII HindII HpaI KpnI MaeI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SalI SapI SexAI SfuI SgrAI SnaBI SpeI SspBI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 3564 GACGTC GACGT^C AflIII 3198 ACATGT A^C[GA][CT]GT Aor51HI 1671 AGCGCT AGC^GCT AseI 1398 ATTAAT AT^TAAT AsnI 1398 ATTAAT AT^TAAT BbeI 3117 GGCGCC GGCGC^C BbrPI 85 CACGTG CAC^GTG BcgI 1874 CGAGTCCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4326 GGATAC GTATCC(6/5) BlpI 4009 GCTGAGC GC^T[ACGT]AGC Bpu1102I 4009 GCTGAGC GC^T[ACGT]AGC BsaAI 85 CACGTG [CT]AC^GT[GA] BsaBI 1321 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1870 GAGACG CGTCTC(1/5) BspDI 2398 ATCGAT AT^CGAT BspLU11I 3198 ACATGT A^CATGT BsrDI 203 GCAATG GCAATG(2/0) BssSI 494 CACGAG CACGAG(-5/-1) CelII 4009 GCTGAGC GC^T[ACGT]AGC ClaI 2398 ATCGAT AT^CGAT DrdI 3660 GACGCCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1671 AGCGCT AGC^GCT FseI 4016 GGCCGGCC GGCCGG^CC HindIII 1838 AAGCTT A^AGCTT KasI 3117 GGCGCC G^GCGCC KspI 1853 CCGCGG CCGC^GG MamI 1321 GATCGGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 3117 GGCGCC GG^CGCC NcoI 1744 CCATGG C^CATGG PmaCI 85 CACGTG CAC^GTG PmlI 85 CACGTG CAC^GTG PshBI 1398 ATTAAT AT^TAAT SacII 1853 CCGCGG CCGC^GG SbfI 3440 CCTGCAGG CCTGCA^GG ScaI 638 AGTACT AGT^ACT SfoI 3117 GGCGCC GGC^GCC Sse8387I 3440 CCTGCAGG CCTGCA^GG SspI 802 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccI 274 GTCTAC GT^[AC][GT]AC 1126 GTCTAC AccIII 839 TCCGGA T^CCGGA 1514 TCCGGA AcsI 1006 AAATTC [GA]^AATT[CT] 1368 GAATTT ApoI 1006 AAATTC [GA]^AATT[CT] 1368 GAATTT BglII 92 AGATCT A^GATCT 3038 AGATCT BseAI 839 TCCGGA T^CCGGA 1514 TCCGGA BspEI 839 TCCGGA T^CCGGA 1514 TCCGGA DraI 1273 TTTAAA TTT^AAA 1371 TTTAAA MroI 839 TCCGGA T^CCGGA 1514 TCCGGA PaeR7I 109 CTCGAG C^TCGAG 634 CTCGAG PflMI 2757 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4291 CCAGACTCTGG PshAI 2594 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3207 GACCAGTGTC SmaI 2732 CCCGGG CCC^GGG 2826 CCCGGG SphI 1064 GCATGC GCATG^C 4371 GCATGC Van91I 2757 CCAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4291 CCAGACTCTGG XhoI 109 CTCGAG C^TCGAG 634 CTCGAG XmaCI 2732 CCCGGG C^CCGGG 2826 CCCGGG XmaI 2732 CCCGGG C^CCGGG 2826 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 135 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AspI AvaI AvaII BalI BamHI BanI BanII BbsI BbvCI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfiI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XcmI XhoII