################################## # NO CUT ENZYMES ( 193 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI Fnu4HI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII ItaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MfeI MluI MluNI MroI MscI MseI MspA1I MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AcsI 226 AAATTC [GA]^AATT[CT] AluI 384 AGCT AG^CT ApoI 226 AAATTC [GA]^AATT[CT] AspHI 446 GAGCAC G[AT]GC[AT]^C AvaII 441 GGTCC G^G[AT]CC BbvCI 15 CCTCAGC CCTCAGC(-5/-2) BmyI 446 GAGCAC G[AGT]GC[ACT]^C BpmI 306 CTGGAG CTGGAG(16/14) BsaBI 196 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 292 GAGGAG GAGGAG(10/8) BsiHKAI 446 GAGCAC G[AT]GC[AT]^C Bsp1286I 446 GAGCAC G[AGT]GC[ACT]^C BspHI 113 TCATGA T^CATGA BstBI 229 TTCGAA TT^CGAA BstF5I 182 GGATG GGATG(2/0) BstXI 57 CCACCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 332 AGATCC [GA]^GATC[CT] Cac8I 312 GCCAGC GC[ACGT]^[ACGT]GC Cfr13I 441 GGTCC G^G[ACGT]CC DsaI 154 CCGTGG C^C[GA][CT]GG FokI 182 GGATG GGATG(9/13) HphI 246 TCACC GGTGA(8/7) MamI 196 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 208 TCTTC GAAGA(8/7) MflI 332 AGATCC [GA]^GATC[CT] NlaIV 440 GGGTCC GG[ACGT]^[ACGT]CC NspV 229 TTCGAA TT^CGAA RcaI 113 TCATGA T^CATGA Sau96I 441 GGTCC G^G[ACGT]CC SfaNI 183 GATGC GCATC(5/9) SfuI 229 TTCGAA TT^CGAA SmlI 386 CTCAAG C^T[CT][GA]AG StuI 395 AGGCCT AGG^CCT TaqI 230 TCGA T^CGA Tsp509I 227 AATT ^AATT TspRI 272 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 230 TCGA T^CGA XhoII 332 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 258 GTAC GT^AC 429 GTAC AlwI 333 GATCC GGATC(4/5) 8 GGATC BsaJI 29 CCCAGG C^C[ACGT][ACGT]GG 154 CCGTGG BsiYI 29 CCCAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 154 CCGTGGGAAGG BslI 29 CCCAGGAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 154 CCGTGGGAAGG BsrI 119 ACTGG ACTGG(1/-1) 305 ACTGG BssKI 30 CCAGG ^CC[ACGT]GG 136 CCAGG BstNI 30 CCAGG CC^[AT]GG 136 CCAGG CfoI 128 GCGC GCG^C 466 GCGC DdeI 16 CTCAG C^T[ACGT]AG 288 CTTAG DpnII 9 GATC ^GATC 333 GATC EcoRII 30 CCAGG ^CC[AT]GG 136 CCAGG HaeIII 311 GGCC GG^CC 396 GGCC HhaI 128 GCGC GCG^C 466 GCGC HinP1I 128 GCGC G^CGC 466 GCGC HinfI 23 GACTC G^A[ACGT]TC 150 GACTC MboI 9 GATC ^GATC 333 GATC MslI 215 CACCAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 247 CACCCGAATG MvaI 30 CCAGG CC^[AT]GG 136 CCAGG NdeII 9 GATC ^GATC 333 GATC NlaIII 44 CATG CATG^ 114 CATG PleI 23 GACTC GAGTC(4/5) 150 GACTC RsaI 258 GTAC GT^AC 429 GTAC Sau3AI 9 GATC ^GATC 333 GATC ScrFI 30 CCAGG CC^[ACGT]GG 136 CCAGG ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### BsmFI MnlI