################################## # NO CUT ENZYMES ( 142 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BstBI BstEII BstPI BstUI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 441 GTATAC GT^[AC][GT]AC AcyI 15 GGCGTC G[GA]^CG[CT]C AfaI 136 GTAC GT^AC AlwNI 1610 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 1165 CTCGGG C^[CT]CG[GA]G BamHI 758 GGATCC G^GATCC BanI 88 GGCACC G^G[CT][GA]CC BcgI 511 CGATTGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 5 CCGGG CC^[GC]GG BglI 1584 GCCTTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 15 GGCGTC G[GA]^CG[CT]C BsmFI 633 GTCCC GGGAC(10/14) BsoBI 1165 CTCGGG C^[CT]CG[GA]G BspMI 779 ACCTGC ACCTGC(4/8) Bst1107I 441 GTATAC GTA^TAC BstAPI 865 GCATTTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1589 CCAGGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 441 GTATAC GTA^TAC DrdI 592 GACAGAGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1301 CCGTGG C^C[GA][CT]GG EarI 1195 CTCTTC CTCTTC(1/4) EcoRI 415 GAATTC G^AATTC EcoRV 394 GATATC GAT^ATC HapII 5 CCGG C^CGG Hin1I 15 GGCGTC G[GA]^CG[CT]C HpaII 5 CCGG C^CGG Ksp632I 1195 CTCTTC CTCTTC(1/4) MaeII 26 ACGT A^CGT MseI 343 TTAA T^TAA MspI 5 CCGG C^CGG NciI 5 CCGGG CC^[GC]GG PflMI 1025 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1092 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 385 CTGCAG CTGCA^G RsaI 136 GTAC GT^AC SacI 1192 GAGCTC GAGCT^C SapI 1194 GCTCTTC GCTCTTC(1/4) SphI 1181 GCATGC GCATG^C StuI 1591 AGGCCT AGG^CCT TaqI 1090 TCGA T^CGA Tru9I 343 TTAA T^TAA TthHB8I 1090 TCGA T^CGA Van91I 1025 CCAAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AspHI 1192 GAGCTC G[AT]GC[AT]^C 1351 GTGCTC AvaII 1098 GGTCC G^G[AT]CC 1104 GGTCC BalI 62 TGGCCA TGG^CCA 1082 TGGCCA BseRI 103 GAGGAG GAGGAG(10/8) 1531 GAGGAG BsiHKAI 1192 GAGCTC G[AT]GC[AT]^C 1351 GTGCTC BsrDI 517 CATTGC GCAATG(2/0) 1247 GCAATG BssSI 890 CACGAG CACGAG(-5/-1) 1528 CACGAG BstYI 758 GGATCC [GA]^GATC[CT] 1175 AGATCC HgaI 1365 GACGC GACGC(5/10) 16 GCGTC HphI 1269 GGTGA GGTGA(8/7) 1355 TCACC MflI 758 GGATCC [GA]^GATC[CT] 1175 AGATCC MluNI 62 TGGCCA TGG^CCA 1082 TGGCCA MscI 62 TGGCCA TGG^CCA 1082 TGGCCA MspA1I 51 CAGCTG C[AC]G^C[GT]G 73 CAGCTG NspI 539 ACATGC [GA]CATG^[CT] 1181 GCATGC PvuII 51 CAGCTG CAG^CTG 73 CAGCTG SfcI 98 CTATAG C^T[GA][CT]AG 385 CTGCAG Tsp45I 1363 GTGAC ^GT[GC]AC 1386 GTCAC XhoII 758 GGATCC [GA]^GATC[CT] 1175 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI BanII BbvI BfaI BmyI BpmI BsaI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI XcmI