(Circular) (Six-base) MAPSORT of: pFN21AB5958.seq Check: 9597 from: 1 to: 6355 pFN21AB5958 6355 bp With 182 enzymes: SgfI * March 28, 2012 12:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4165 278 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2447 3698 1937 Size: 510 1251 4594 Fragments arranged by size: 4594 1251 510 Acc65I G'GTAC_C Cuts at: 3676 3676 Size: 6355 AclI AA'CG_TT Cuts at: 1563 4482 4855 1563 Size: 2919 373 3063 Fragments arranged by size: 3063 2919 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2494 3642 4418 5802 6070 1632 Size: 862 1148 776 1384 268 1917 Fragments arranged by size: 1917 1384 1148 862 776 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6158 AflIII A'CryG_T Cuts at: 1203 5254 6231 1203 Size: 4051 977 1327 Fragments arranged by size: 4051 1327 977 AgeI A'CCGG_T Cuts at: 5186 5186 Size: 6355 AhdI GACnn_n'nnGTC Cuts at: 5084 5084 Size: 6355 AleI CACnn'nnGTG Cuts at: 1166 3365 1166 Size: 2199 4156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6355 AlwNI CAG_nnn'CTG Cuts at: 3620 4098 5670 3620 Size: 478 1572 4305 Fragments arranged by size: 4305 1572 478 ApaLI G'TGCA_C Cuts at: 4412 5568 4412 Size: 1156 5199 ApoI r'AATT_y Cuts at: 1493 2420 2665 2989 3664 3890 4042 6151 1493 Size: 927 245 324 675 226 152 2109 1697 Fragments arranged by size: 2109 1697 927 675 324 245 226 152 AseI AT'TA_AT Cuts at: 160 1032 4909 160 Size: 872 3877 1606 Fragments arranged by size: 3877 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 AvaI C'yCGr_G Cuts at: 1870 1943 3170 3680 1870 Size: 73 1227 510 4545 Fragments arranged by size: 4545 1227 510 73 BamHI G'GATC_C Cuts at: 2763 3685 4052 2763 Size: 922 367 5066 Fragments arranged by size: 5066 922 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 3676 5131 618 Size: 325 217 627 306 1583 1455 1842 Fragments arranged by size: 1842 1583 1455 627 325 306 217 BanII G_rGCy'C Cuts at: 1391 2077 3171 3201 3674 1391 Size: 686 1094 30 473 4072 Fragments arranged by size: 4072 1094 686 473 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5384 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2505 2539 4546 4580 1903 Size: 34 568 34 2007 34 3678 Fragments arranged by size: 3678 2007 568 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4247 5457 4247 Size: 1210 5145 BclI T'GATC_A Cuts at: 1548 1548 Size: 6355 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3595 4966 136 Size: 107 122 71 631 605 1923 1371 1525 Fragments arranged by size: 1923 1525 1371 631 605 122 107 71 BglII A'GATC_T Cuts at: 6350 6350 Size: 6355 BlpI GC'TnA_GC Cuts at: 3772 3772 Size: 6355 Bme1580I G_kGCm'C Cuts at: 1790 4416 5572 1790 Size: 2626 1156 2573 Fragments arranged by size: 2626 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2032 1318 Size: 714 5641 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3174 4086 5044 Size: 416 53 83 399 24 1757 912 958 Cuts at: 5044 442 Size: 1753 Fragments arranged by size: 1757 1753 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6355 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2654 2958 3514 5015 1239 Size: 917 498 304 556 1501 2579 Fragments arranged by size: 2579 1501 917 556 498 304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4480 5345 5643 4480 Size: 865 298 5192 Fragments arranged by size: 5192 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2783 3035 3533 5018 882 Size: 617 1284 252 498 1485 2219 Fragments arranged by size: 2219 1485 1284 617 498 252 BsaAI yAC'GTr Cuts at: 493 1204 4146 493 Size: 711 2942 2702 Fragments arranged by size: 2942 2702 711 BsaBI GATnn'nnATC Cuts at: 6349 6349 Size: 6355 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2021 4162 4544 5179 Size: 53 83 186 936 488 2141 382 635 Cuts at: 5179 275 Size: 1451 Fragments arranged by size: 2141 1451 936 635 488 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4788 5186 5460 5607 6018 4788 Size: 398 274 147 411 5125 Fragments arranged by size: 5125 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2621 2651 1178 Size: 30 165 30 1218 30 4882 Fragments arranged by size: 4882 1218 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 3551 1186 Size: 937 1428 3990 Fragments arranged by size: 3990 1428 937 BseYI C'CCAG_C Cuts at: 2014 2076 2853 2922 5558 6054 2014 Size: 62 777 69 2636 496 2315 Fragments arranged by size: 2636 2315 777 496 69 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5782 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3805 4566 5594 1540 Size: 275 186 1804 761 1028 2301 Fragments arranged by size: 2301 1804 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3201 3360 4416 4501 5572 3201 Size: 159 1056 85 1071 3984 Fragments arranged by size: 3984 1071 1056 159 85 BsmI GAATG_Cn' Cuts at: 3866 3959 3866 Size: 93 6262 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 3171 3201 3360 3674 4416 4501 Size: 399 287 1094 30 159 314 742 85 Cuts at: 4501 5572 1391 Size: 1071 2174 Fragments arranged by size: 2174 1094 1071 742 399 314 287 159 85 30 BspEI T'CCGG_A Cuts at: 6018 6018 Size: 6355 BspHI T'CATG_A Cuts at: 4244 5974 4244 Size: 1730 4625 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2793 3710 844 Size: 1949 917 3489 Fragments arranged by size: 3489 1949 917 BsrBI CCG'CTC Cuts at: 1394 1823 4242 1394 Size: 429 2419 3507 Fragments arranged by size: 3507 2419 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2521 3259 4850 5024 65 Size: 1183 1273 738 1591 174 1396 Fragments arranged by size: 1591 1396 1273 1183 738 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4999 5186 1700 Size: 110 3189 187 2869 Fragments arranged by size: 3189 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6355 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6355 BssSI C'ACGA_G Cuts at: 2895 3533 4415 5427 2895 Size: 638 882 1012 3823 Fragments arranged by size: 3823 1012 882 638 BstAPI GCAn_nnn'nTGC Cuts at: 794 2876 794 Size: 2082 4273 BstBI TT'CG_AA Cuts at: 5159 5159 Size: 6355 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6355 BstXI CCAn_nnnn'nTGG Cuts at: 3601 3601 Size: 6355 BstYI r'GATC_y Cuts at: 1972 2763 3180 3685 4052 4437 4454 5906 5992 Size: 791 417 505 367 385 17 1452 86 Cuts at: 5992 6004 6350 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 791 505 417 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2448 2448 Size: 6355 BtgI C'CryG_G Cuts at: 513 1065 2004 3306 513 Size: 552 939 1302 3562 Fragments arranged by size: 3562 1302 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5616 Fragments arranged by size: 5616 683 56 BtsI GCAGTG_nn' Cuts at: 3871 4665 4685 3871 Size: 794 20 5541 Fragments arranged by size: 5541 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6355 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2667 2702 529 Size: 35 2103 35 4182 Fragments arranged by size: 4182 2103 35 35 DraI TTT'AAA Cuts at: 3659 3659 Size: 6355 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2603 5362 809 Size: 507 1287 2759 1802 Fragments arranged by size: 2759 1802 1287 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2067 3087 Size: 54 1425 50 275 18 177 60 1020 Cuts at: 3087 3402 3802 4691 6320 8 Size: 315 400 889 1629 43 Fragments arranged by size: 1629 1425 1020 889 400 315 275 177 60 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3802 1537 Size: 275 1990 4090 Fragments arranged by size: 4090 1990 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3206 4285 1353 Size: 1853 1079 3423 Fragments arranged by size: 3423 1853 1079 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4942 5316 5462 387 Size: 1064 138 3353 374 146 1280 Fragments arranged by size: 3353 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3199 3199 Size: 6355 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2494 2654 2958 3514 3642 4418 Size: 393 524 338 160 304 556 128 776 Cuts at: 4418 5015 5802 6070 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 776 597 556 524 393 338 304 268 160 128 EcoRI G'AATT_C Cuts at: 2420 3664 4042 2420 Size: 1244 378 4733 Fragments arranged by size: 4733 1244 378 EcoRV GAT'ATC Cuts at: 2401 2401 Size: 6355 FspI TGC'GCA Cuts at: 4861 4861 Size: 6355 HaeII r_GCGC'y Cuts at: 1678 5502 1678 Size: 3824 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2621 2653 3274 3306 4996 Size: 32 135 32 1049 32 621 32 1690 Cuts at: 4996 5028 5070 5102 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1690 1049 621 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3699 3945 669 Size: 988 281 1761 246 3079 Fragments arranged by size: 3079 1761 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6355 HpaI GTT'AAC Cuts at: 3945 3945 Size: 6355 KpnI G_GTAC'C Cuts at: 3680 3680 Size: 6355 MfeI C'AATT_G Cuts at: 3954 3954 Size: 6355 MluI A'CGCG_T Cuts at: 6231 6231 Size: 6355 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2329 5469 5653 1305 Size: 1024 3140 184 2007 Fragments arranged by size: 3140 2007 1024 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2069 3089 10 Size: 54 1425 343 237 1020 3276 Fragments arranged by size: 3276 1425 1020 343 237 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 3328 3365 3413 4313 4672 4831 Size: 648 1635 527 37 48 900 359 159 Cuts at: 4831 518 Size: 2042 Fragments arranged by size: 2042 1635 900 648 527 359 159 48 37 MspA1I CmG'CkG Cuts at: 1606 2058 2080 3771 4448 5596 5841 1606 Size: 452 22 1691 677 1148 245 2120 Fragments arranged by size: 2120 1691 1148 677 452 245 22 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4864 Fragments arranged by size: 4864 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6355 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6355 NotI GC'GGCC_GC Cuts at: 3802 3802 Size: 6355 NspI r_CATG'y Cuts at: 1132 1229 2548 3190 3713 5258 1132 Size: 97 1319 642 523 1545 2229 Fragments arranged by size: 2229 1545 1319 642 523 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6355 PciI A'CATG_T Cuts at: 5254 5254 Size: 6355 PflMI CCAn_nnn'nTGG Cuts at: 1108 3036 1108 Size: 1928 4427 PfoI T'CCnGG_A Cuts at: 2172 2172 Size: 6355 PmeI GTTT'AAAC Cuts at: 3659 3659 Size: 6355 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4424 4456 5937 5969 4424 Size: 32 1481 32 4810 Fragments arranged by size: 4810 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3101 1613 Size: 1488 4867 PsiI TTA'TAA Cuts at: 3925 3925 Size: 6355 PstI C_TGCA'G Cuts at: 830 2394 3707 830 Size: 1564 1313 3478 Fragments arranged by size: 3478 1564 1313 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6355 PvuII CAG'CTG Cuts at: 2058 2080 2058 Size: 22 6333 SacI G_AGCT'C Cuts at: 3201 3201 Size: 6355 SalI G'TCGA_C Cuts at: 1936 3697 1936 Size: 1761 4594 SapI GCTCTTCn'nnn_ Cuts at: 3206 3206 Size: 6355 SbfI CC_TGCA'GG Cuts at: 3707 3707 Size: 6355 ScaI AGT'ACT Cuts at: 4603 6315 4603 Size: 1712 4643 SfcI C'TryA_G Cuts at: 826 1045 2103 2390 3703 4838 5519 5710 826 Size: 219 1058 287 1313 1135 681 191 1471 Fragments arranged by size: 1471 1313 1135 1058 681 287 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6355 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6355 SmaI CCC'GGG Cuts at: 1872 3682 1872 Size: 1810 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4459 5360 5622 820 Size: 197 926 2516 901 262 1553 Fragments arranged by size: 2516 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4146 493 Size: 3653 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6355 SphI G_CATG'C Cuts at: 1132 3190 3713 1132 Size: 2058 523 3774 Fragments arranged by size: 3774 2058 523 SspI AAT'ATT Cuts at: 5 52 4279 5 Size: 47 4227 2081 Fragments arranged by size: 4227 2081 47 StuI AGG'CCT Cuts at: 1517 3598 1517 Size: 2081 4274 StyI C'CwwG_G Cuts at: 513 1065 2004 2478 3543 3631 3794 513 Size: 552 939 474 1065 88 163 3074 Fragments arranged by size: 3074 1065 939 552 474 163 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4382 4399 4552 4737 5182 325 Size: 1255 2802 17 153 185 445 1498 Fragments arranged by size: 2802 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2140 4601 6313 Size: 274 80 33 51 1443 163 2461 1712 Cuts at: 6313 96 Size: 138 Fragments arranged by size: 2461 1712 1443 274 163 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3513 3545 6315 6347 3513 Size: 32 2770 32 3521 Fragments arranged by size: 3521 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6355 XbaI T'CTAG_A Cuts at: 3691 3691 Size: 6355 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2077 2233 3097 3412 2011 Size: 66 156 864 315 4954 Fragments arranged by size: 4954 864 315 156 66 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6355 XmaI C'CCGG_G Cuts at: 1870 3680 1870 Size: 1810 4545 XmnI GAAnn'nnTTC Cuts at: 4484 4484 Size: 6355 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4163 276 Size: 53 83 186 936 2629 2468 Fragments arranged by size: 2629 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI