(Linear) (Six-base) MAPSORT of: KIBB5958.seq Check: 5878 from: 1 to: 1650 KIBB5958.orf With 182 enzymes: SgfI * March 22, 2012 15:33 .. AccI GT'mk_AC Cuts at: 0 442 1650 Size: 442 1208 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 489 1637 1650 Size: 489 1148 13 Fragments arranged by size: 1148 489 13 AleI CACnn'nnGTG Cuts at: 0 1360 1650 Size: 1360 290 AlwNI CAG_nnn'CTG Cuts at: 0 1615 1650 Size: 1615 35 ApoI r'AATT_y Cuts at: 0 415 660 984 1650 Size: 415 245 324 666 Fragments arranged by size: 666 415 324 245 AvaI C'yCGr_G Cuts at: 0 1165 1650 Size: 1165 485 BamHI G'GATC_C Cuts at: 0 758 1650 Size: 758 892 BanI G'GyrC_C Cuts at: 0 88 1650 Size: 88 1562 BanII G_rGCy'C Cuts at: 0 72 1166 1196 1650 Size: 72 1094 30 454 Fragments arranged by size: 1094 454 72 30 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 500 534 1650 Size: 500 34 1116 Fragments arranged by size: 1116 500 34 BglI GCCn_nnn'nGGC Cuts at: 0 1590 1650 Size: 1590 60 BmgBI CAC'GTC Cuts at: 0 27 1650 Size: 27 1623 BmrI ACTGGGnnnn_n' Cuts at: 0 1169 1650 Size: 1169 481 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 649 953 1509 1650 Size: 151 498 304 556 141 Fragments arranged by size: 556 498 304 151 141 BsaI GGTCTCn'nnnn_ Cuts at: 0 778 1030 1528 1650 Size: 778 252 498 122 Fragments arranged by size: 778 498 252 122 BsaHI Gr'CG_yC Cuts at: 0 16 1650 Size: 16 1634 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 616 646 1650 Size: 616 30 1004 Fragments arranged by size: 1004 616 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 118 1546 1650 Size: 118 1428 104 Fragments arranged by size: 1428 118 104 BseYI C'CCAG_C Cuts at: 0 9 71 848 917 1650 Size: 9 62 777 69 733 Fragments arranged by size: 777 733 69 62 9 BsiHKAI G_wGCw'C Cuts at: 0 1196 1355 1650 Size: 1196 159 295 Fragments arranged by size: 1196 295 159 Bsp1286I G_dGCh'C Cuts at: 0 72 1166 1196 1355 1650 Size: 72 1094 30 159 295 Fragments arranged by size: 1094 295 159 72 30 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 788 1650 Size: 788 862 BsrDI GCAATG_nn' Cuts at: 0 516 1254 1650 Size: 516 738 396 Fragments arranged by size: 738 516 396 BssSI C'ACGA_G Cuts at: 0 890 1528 1650 Size: 890 638 122 Fragments arranged by size: 890 638 122 BstAPI GCAn_nnn'nTGC Cuts at: 0 871 1650 Size: 871 779 BstXI CCAn_nnnn'nTGG Cuts at: 0 1596 1650 Size: 1596 54 BstYI r'GATC_y Cuts at: 0 758 1175 1650 Size: 758 417 475 Fragments arranged by size: 758 475 417 BstZ17I GTA'TAC Cuts at: 0 443 1650 Size: 443 1207 BtgI C'CryG_G Cuts at: 0 1301 1650 Size: 1301 349 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 662 697 1650 Size: 662 35 953 Fragments arranged by size: 953 662 35 DrdI GACnn_nn'nnGTC Cuts at: 0 598 1650 Size: 598 1052 EaeI y'GGCC_r Cuts at: 0 2 62 1082 1397 1650 Size: 2 60 1020 315 253 Fragments arranged by size: 1020 315 253 60 2 EarI CTCTTCn'nnn_ Cuts at: 0 1201 1650 Size: 1201 449 EcoICRI GAG'CTC Cuts at: 0 1194 1650 Size: 1194 456 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 151 489 649 953 1509 1637 1650 Size: 151 338 160 304 556 128 13 Fragments arranged by size: 556 338 304 160 151 128 13 EcoRI G'AATT_C Cuts at: 0 415 1650 Size: 415 1235 EcoRV GAT'ATC Cuts at: 0 396 1650 Size: 396 1254 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 616 648 1269 1301 1650 Size: 616 32 621 32 349 Fragments arranged by size: 621 616 349 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 324 1650 Size: 324 1326 MscI TGG'CCA Cuts at: 0 64 1084 1650 Size: 64 1020 566 Fragments arranged by size: 1020 566 64 MslI CAynn'nnrTG Cuts at: 0 796 1323 1360 1408 1650 Size: 796 527 37 48 242 Fragments arranged by size: 796 527 242 48 37 MspA1I CmG'CkG Cuts at: 0 53 75 1650 Size: 53 22 1575 Fragments arranged by size: 1575 53 22 NspI r_CATG'y Cuts at: 0 543 1185 1650 Size: 543 642 465 Fragments arranged by size: 642 543 465 PflMI CCAn_nnn'nTGG Cuts at: 0 1031 1650 Size: 1031 619 PfoI T'CCnGG_A Cuts at: 0 167 1650 Size: 167 1483 PshAI GACnn'nnGTC Cuts at: 0 1096 1650 Size: 1096 554 PstI C_TGCA'G Cuts at: 0 389 1650 Size: 389 1261 PvuII CAG'CTG Cuts at: 0 53 75 1650 Size: 53 22 1575 Fragments arranged by size: 1575 53 22 SacI G_AGCT'C Cuts at: 0 1196 1650 Size: 1196 454 SapI GCTCTTCn'nnn_ Cuts at: 0 1201 1650 Size: 1201 449 SfcI C'TryA_G Cuts at: 0 98 385 1650 Size: 98 287 1265 Fragments arranged by size: 1265 287 98 SphI G_CATG'C Cuts at: 0 1185 1650 Size: 1185 465 StuI AGG'CCT Cuts at: 0 1593 1650 Size: 1593 57 StyI C'CwwG_G Cuts at: 0 473 1538 1626 1650 Size: 473 1065 88 24 Fragments arranged by size: 1065 473 88 24 TatI w'GTAC_w Cuts at: 0 135 1650 Size: 135 1515 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1508 1540 1650 Size: 1508 32 110 Fragments arranged by size: 1508 110 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 72 228 1092 1407 1650 Size: 72 156 864 315 243 Fragments arranged by size: 864 315 243 156 72 Enzymes that do cut: AccI AcuI AleI AlwNI ApoI AvaI BamHI BanI BanII BcgI BglI BmgBI BmrI BpmI BsaI BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BssSI BstAPI BstXI BstYI BstZ17I BtgI CspCI DrdI EaeI EarI EcoICRI Eco57MI EcoRI EcoRV Hin4I MmeI MscI MslI MspA1I NspI PflMI PfoI PshAI PstI PvuII SacI SapI SfcI SphI StuI StyI TatI TstI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII Bsu36I BtgZI BtsI ClaI DraI DraIII EagI EciI EcoNI EcoO109I FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI XmnI ZraI