Restriction map for
pFN21AE0468
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# NO CUT ENZYMES ( 116 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AocI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbrPI BcgI BciVI BfrI
BglI BlnI BsaAI BsaBI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstF5I BstZ17I Bsu36I ClaI
CpoI DraI DraIII EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoRI
EcoRV EcoT22I FokI FseI FspI
HgaI HincII HindII HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 603 GACGTC GACGT^C
AccII 1110 CGCG CG^CG
AcsI 281 AAATTC [GA]^AATT[CT]
AflIII 224 ACATGT A^C[GA][CT]GT
AhdI 68 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 211 GTGCAC G^TGCAC
AlwNI 65 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 211 GTGCAC G^TGCAC
ApoI 281 AAATTC [GA]^AATT[CT]
AspEI 68 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 211 GTGCAC G[AT]GC[AT]^C
BalI 17 TGGCCA TGG^CCA
BamHI 32 GGATCC G^GATCC
BbsI 673 GTCTTC GAAGAC(2/6)
BbvCI 1019 CCTCAGC CCTCAGC(-5/-2)
BclI 314 TGATCA T^GATCA
BglII 386 AGATCT A^GATCT
BlpI 845 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 845 GCTCAGC GC^T[ACGT]AGC
BpuAI 673 GTCTTC GAAGAC(2/6)
BsiHKAI 211 GTGCAC G[AT]GC[AT]^C
BsmI 56 GCATTC GAATGC(1/-1)
BspLU11I 224 ACATGT A^CATGT
BspMI 69 ACCTGC ACCTGC(4/8)
BsrFI 52 GCCGGC [GA]^CCGG[CT]
BstAPI 418 GCAAAGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1110 CGCG CG^CG
BstXI 404 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 845 GCTCAGC GC^T[ACGT]AGC
Cfr10I 52 GCCGGC [GA]^CCGG[CT]
DrdI 988 GACAGCCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 17 TGGCCA [CT]^GGCC[GA]
Eam1105I 68 GACCTGCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 114 GAAGAG CTCTTC(1/4)
FbaI 314 TGATCA T^GATCA
Ksp632I 114 GAAGAG CTCTTC(1/4)
MluNI 17 TGGCCA TGG^CCA
MscI 17 TGGCCA TGG^CCA
MslI 525 CATCAACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 962 CAGCTG C[AC]G^C[GT]G
MvnI 1110 CGCG CG^CG
NaeI 52 GCCGGC GCC^GGC
NgoMIV 52 GCCGGC G^CCGGC
PvuII 962 CAGCTG CAG^CTG
SapI 114 GAAGAGC GCTCTTC(1/4)
SfaNI 941 GCATC GCATC(5/9)
SmaI 1064 CCCGGG CCC^GGG
TfiI 108 GATTC G^A[AT]TC
XcmI 434 CCACTGCAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1064 CCCGGG C^CCGGG
XmaI 1064 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AfaI 250 GTAC GT^AC
519 GTAC
ApaI 789 GGGCCC GGGCC^C
1060 GGGCCC
AvaI 976 CCCGAG C^[CT]CG[GA]G
1064 CCCGGG
BanII 789 GGGCCC G[GA]GC[CT]^C
1060 GGGCCC
BfaI 263 CTAG C^TAG
781 CTAG
BseRI 457 GAGGAG GAGGAG(10/8)
460 GAGGAG
BsmFI 722 GGGAC GGGAC(10/14)
806 GTCCC
BsoBI 976 CCCGAG C^[CT]CG[GA]G
1064 CCCGGG
BstEII 996 GGTCACC G^GT[ACGT]ACC
1035 GGTCACC
BstPI 996 GGTCACC G^GT[ACGT]ACC
1035 GGTCACC
DsaI 167 CCGTGG C^C[GA][CT]GG
288 CCGTGG
EcoO65I 996 GGTCACC G^GT[ACGT]ACC
1035 GGTCACC
MaeI 263 CTAG C^TAG
781 CTAG
MaeII 521 ACGT A^CGT
604 ACGT
MseI 44 TTAA T^TAA
1043 TTAA
PstI 255 CTGCAG CTGCA^G
437 CTGCAG
RsaI 250 GTAC GT^AC
519 GTAC
SfcI 255 CTGCAG C^T[GA][CT]AG
437 CTGCAG
SmlI 193 CTCAAG C^T[CT][GA]AG
907 CTTGAG
SphI 119 GCATGC GCATG^C
198 GCATGC
Tru9I 44 TTAA T^TAA
1043 TTAA
Tsp509I 79 AATT ^AATT
282 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AluI AlwI AvaII
BanI BbeI BbvI BcnI BmyI
BpmI BsaHI BsaI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
EcoT14I FauI Fnu4HI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
Sau3AI Sau96I ScrFI SfoI StyI
TseI Tsp45I TspRI XhoII