(Circular) (Six-base) MAPSORT of: pFN21AE0468.seq Check: 8646 from: 1 to: 5860 pFN21AE0468 5860 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2612 3670 278 Size: 53 83 186 936 1076 1058 2468 Fragments arranged by size: 2468 1076 1058 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3203 1937 Size: 1266 4594 Acc65I G'GTAC_C Cuts at: 3181 3181 Size: 5860 AclI AA'CG_TT Cuts at: 1563 3987 4360 1563 Size: 2424 373 3063 Fragments arranged by size: 3063 2424 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2665 2798 3923 5307 5575 1632 Size: 1033 133 1125 1384 268 1917 Fragments arranged by size: 1917 1384 1125 1033 268 133 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5663 AflIII A'CryG_T Cuts at: 1203 2229 4759 5736 1203 Size: 1026 2530 977 1327 Fragments arranged by size: 2530 1327 1026 977 AgeI A'CCGG_T Cuts at: 4691 4691 Size: 5860 AhdI GACnn_n'nnGTC Cuts at: 2078 4589 2078 Size: 2511 3349 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5860 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2604 1184 Size: 1420 4440 AlwNI CAG_nnn'CTG Cuts at: 2075 3603 5175 2075 Size: 1528 1572 2760 Fragments arranged by size: 2760 1572 1528 ApaI G_GGCC'C Cuts at: 2798 3069 2798 Size: 271 5589 ApaLI G'TGCA_C Cuts at: 2216 3917 5073 2216 Size: 1701 1156 3003 Fragments arranged by size: 3003 1701 1156 ApoI r'AATT_y Cuts at: 1493 2286 3169 3395 3547 5656 1493 Size: 793 883 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 793 226 152 AseI AT'TA_AT Cuts at: 160 1032 4414 160 Size: 872 3382 1606 Fragments arranged by size: 3382 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 AvaI C'yCGr_G Cuts at: 1870 1943 2981 3069 3185 1870 Size: 73 1038 88 116 4545 Fragments arranged by size: 4545 1038 116 88 73 BamHI G'GATC_C Cuts at: 2037 3190 3557 2037 Size: 1153 367 4340 Fragments arranged by size: 4340 1153 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2297 2367 2493 2546 2630 Size: 325 217 627 510 70 126 53 84 Cuts at: 2630 2883 3098 3181 4636 618 Size: 253 215 83 1455 1842 Fragments arranged by size: 1842 1455 627 510 325 253 217 215 126 84 83 70 53 BanII G_rGCy'C Cuts at: 1391 2798 3069 3179 1391 Size: 1407 271 110 4072 Fragments arranged by size: 4072 1407 271 110 BbeI G_GCGC'C Cuts at: 2371 2497 2634 2887 2371 Size: 126 137 253 5344 Fragments arranged by size: 5344 253 137 126 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2671 928 Size: 971 772 4117 Fragments arranged by size: 4117 971 772 BbvCI CC'TCA_GC Cuts at: 3025 3025 Size: 5860 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3139 3173 4051 4085 1903 Size: 34 1202 34 878 34 3678 Fragments arranged by size: 3678 1202 878 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3752 4962 3752 Size: 1210 4650 BclI T'GATC_A Cuts at: 1548 2319 1548 Size: 771 5089 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4471 136 Size: 107 122 71 631 605 2799 1525 Fragments arranged by size: 2799 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2391 5855 2391 Size: 3464 2396 BlpI GC'TnA_GC Cuts at: 2851 3277 2851 Size: 426 5434 Bme1580I G_kGCm'C Cuts at: 1790 2220 2798 2982 3069 3103 3122 3921 5077 Size: 430 578 184 87 34 19 799 1156 Cuts at: 5077 1790 Size: 2573 Fragments arranged by size: 2573 1156 799 578 430 184 87 34 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5860 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2969 3152 3591 Size: 416 53 83 399 24 1552 183 439 Cuts at: 3591 4549 442 Size: 958 1753 Fragments arranged by size: 1753 1552 958 439 416 399 183 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5860 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 2594 3067 4520 1239 Size: 813 542 473 1453 2579 Fragments arranged by size: 2579 1453 813 542 473 Bpu10I CC'TnA_GC Cuts at: 3025 3025 Size: 5860 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2183 2933 3985 4850 5148 2183 Size: 750 1052 865 298 2895 Fragments arranged by size: 2895 1052 865 750 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2014 2603 2630 4523 882 Size: 617 515 589 27 1893 2219 Fragments arranged by size: 2219 1893 617 589 515 27 BsaAI yAC'GTr Cuts at: 493 1204 3651 493 Size: 711 2447 2702 Fragments arranged by size: 2702 2447 711 BsaBI GATnn'nnATC Cuts at: 5854 5854 Size: 5860 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2368 2494 2609 2631 Size: 53 83 186 936 835 126 115 22 Cuts at: 2631 2884 3667 4049 4684 275 Size: 253 783 382 635 1451 Fragments arranged by size: 1451 936 835 783 635 382 253 186 126 115 83 53 22 BsaWI w'CCGG_w Cuts at: 4293 4691 4965 5112 5523 4293 Size: 398 274 147 411 4630 Fragments arranged by size: 4630 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2507 2537 2918 2948 1178 Size: 30 165 30 1104 30 381 30 4090 Fragments arranged by size: 4090 1104 381 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2480 1186 Size: 1291 3 4566 Fragments arranged by size: 4566 1291 3 BseYI C'CCAG_C Cuts at: 2161 2351 2797 2814 5063 5559 2161 Size: 190 446 17 2249 496 2462 Fragments arranged by size: 2462 2249 496 446 190 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5287 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3310 4071 5099 1540 Size: 275 186 1309 761 1028 2301 Fragments arranged by size: 2301 1309 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2220 3921 4006 5077 2220 Size: 1701 85 1071 3003 Fragments arranged by size: 3003 1701 1071 85 BsmI GAATG_Cn' Cuts at: 2061 3371 3464 2061 Size: 1310 93 4457 Fragments arranged by size: 4457 1310 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2220 2798 2982 3069 3103 3122 3179 Size: 399 430 578 184 87 34 19 57 Cuts at: 3179 3921 4006 5077 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 578 430 399 184 87 85 57 34 19 BspEI T'CCGG_A Cuts at: 5523 5523 Size: 5860 BspHI T'CATG_A Cuts at: 3749 5479 3749 Size: 1730 4130 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2083 3215 844 Size: 1239 1132 3489 Fragments arranged by size: 3489 1239 1132 BsrBI CCG'CTC Cuts at: 1394 1823 3747 1394 Size: 429 1924 3507 Fragments arranged by size: 3507 1924 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4355 4529 65 Size: 1183 3107 174 1396 Fragments arranged by size: 3107 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2057 4504 4691 1700 Size: 110 247 2447 187 2869 Fragments arranged by size: 2869 2447 247 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5860 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5860 BssSI C'ACGA_G Cuts at: 3920 4932 3920 Size: 1012 4848 BstAPI GCAn_nnn'nTGC Cuts at: 794 2429 794 Size: 1635 4225 BstBI TT'CG_AA Cuts at: 4664 4664 Size: 5860 BstEII G'GTnAC_C Cuts at: 1184 3001 3040 1184 Size: 1817 39 4004 Fragments arranged by size: 4004 1817 39 BstXI CCAn_nnnn'nTGG Cuts at: 2416 2416 Size: 5860 BstYI r'GATC_y Cuts at: 1972 2037 2134 2391 2568 2716 3190 3557 3942 Size: 65 97 257 177 148 474 367 385 Cuts at: 3942 3959 5411 5497 5509 5855 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 474 385 367 346 257 177 148 97 86 65 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2172 2293 513 Size: 552 939 168 121 4080 Fragments arranged by size: 4080 939 552 168 121 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2173 487 Size: 683 56 947 4174 Fragments arranged by size: 4174 947 683 56 BtsI GCAGTG_nn' Cuts at: 2439 2502 2876 3376 4170 4190 2439 Size: 63 374 500 794 20 4109 Fragments arranged by size: 4109 794 500 374 63 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5860 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5825 DraI TTT'AAA Cuts at: 3164 3164 Size: 5860 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2999 4867 809 Size: 507 1683 1868 1802 Fragments arranged by size: 1868 1802 1683 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 3307 4196 Size: 54 1425 50 275 18 192 1285 889 Cuts at: 4196 5825 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1285 889 275 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3307 1537 Size: 275 1495 4090 Fragments arranged by size: 4090 1495 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2114 3790 1353 Size: 761 1676 3423 Fragments arranged by size: 3423 1676 761 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4447 4821 4967 387 Size: 1064 138 2858 374 146 1280 Fragments arranged by size: 2858 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 2594 2665 2798 3067 3923 4520 Size: 393 420 542 71 133 269 856 597 Cuts at: 4520 5307 5575 1239 Size: 787 268 1524 Fragments arranged by size: 1524 856 787 597 542 420 393 269 268 133 71 EcoO109I rG'GnC_Cy Cuts at: 2072 2773 2794 3065 3066 3091 3153 2072 Size: 701 21 271 1 25 62 4779 Fragments arranged by size: 4779 701 271 62 25 21 1 EcoRI G'AATT_C Cuts at: 3169 3547 3169 Size: 378 5482 FspI TGC'GCA Cuts at: 4366 4366 Size: 5860 HaeII r_GCGC'y Cuts at: 1678 2371 2497 2634 2887 2894 5007 1678 Size: 693 126 137 253 7 2113 2531 Fragments arranged by size: 2531 2113 693 253 137 126 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2918 2950 4501 Size: 32 135 32 492 32 822 32 1551 Cuts at: 4501 4533 4575 4607 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1551 822 492 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3204 3450 669 Size: 988 281 1266 246 3079 Fragments arranged by size: 3079 1266 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5860 HpaI GTT'AAC Cuts at: 3450 3450 Size: 5860 KasI G'GCGC_C Cuts at: 2367 2493 2630 2883 2367 Size: 126 137 253 5344 Fragments arranged by size: 5344 253 137 126 KpnI G_GTAC'C Cuts at: 3185 3185 Size: 5860 MfeI C'AATT_G Cuts at: 3459 3459 Size: 5860 MluI A'CGCG_T Cuts at: 5736 5736 Size: 5860 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 4974 5158 1305 Size: 1128 2541 184 2007 Fragments arranged by size: 2541 2007 1128 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2024 10 Size: 54 1425 343 192 3846 Fragments arranged by size: 3846 1425 343 192 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 3818 4177 4336 518 Size: 648 1368 1284 359 159 2042 Fragments arranged by size: 2042 1368 1284 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2969 3276 3953 5101 5346 1606 Size: 1363 307 677 1148 245 2120 Fragments arranged by size: 2120 1363 1148 677 307 245 NaeI GCC'GGC Cuts at: 2059 2059 Size: 5860 NarI GG'CG_CC Cuts at: 2368 2494 2631 2884 2368 Size: 126 137 253 5344 Fragments arranged by size: 5344 253 137 126 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4369 Fragments arranged by size: 4369 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5860 NgoMIV G'CCGG_C Cuts at: 2057 2057 Size: 5860 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5860 NotI GC'GGCC_GC Cuts at: 3307 3307 Size: 5860 NspI r_CATG'y Cuts at: 1132 1229 2128 2207 2233 2539 3218 4763 1132 Size: 97 899 79 26 306 679 1545 2229 Fragments arranged by size: 2229 1545 899 679 306 97 79 26 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5860 PciI A'CATG_T Cuts at: 2229 4759 2229 Size: 2530 3330 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5860 PfoI T'CCnGG_A Cuts at: 2068 2571 2068 Size: 503 5357 PmeI GTTT'AAAC Cuts at: 3164 3164 Size: 5860 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2327 2359 3929 3961 5442 5474 2327 Size: 32 1570 32 1481 32 2713 Fragments arranged by size: 2713 1570 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 2773 3153 2072 Size: 701 380 4779 Fragments arranged by size: 4779 701 380 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5860 PsiI TTA'TAA Cuts at: 3430 3430 Size: 5860 PspOMI G'GGCC_C Cuts at: 2794 3065 2794 Size: 271 5589 PstI C_TGCA'G Cuts at: 830 2264 2446 3212 830 Size: 1434 182 766 3478 Fragments arranged by size: 3478 1434 766 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5860 PvuII CAG'CTG Cuts at: 2969 2969 Size: 5860 SalI G'TCGA_C Cuts at: 1936 3202 1936 Size: 1266 4594 SapI GCTCTTCn'nnn_ Cuts at: 2114 2114 Size: 5860 SbfI CC_TGCA'GG Cuts at: 3212 3212 Size: 5860 ScaI AGT'ACT Cuts at: 4108 5820 4108 Size: 1712 4148 SfcI C'TryA_G Cuts at: 826 1045 2260 2442 3208 4343 5024 5215 826 Size: 219 1215 182 766 1135 681 191 1471 Fragments arranged by size: 1471 1215 1135 766 681 219 191 182 SfoI GGC'GCC Cuts at: 2369 2495 2632 2885 2369 Size: 126 137 253 5344 Fragments arranged by size: 5344 253 137 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5860 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5860 SmaI CCC'GGG Cuts at: 1872 3071 3187 1872 Size: 1199 116 4545 Fragments arranged by size: 4545 1199 116 SmlI C'TyrA_G Cuts at: 820 1017 1943 2198 2912 3964 4865 5127 820 Size: 197 926 255 714 1052 901 262 1553 Fragments arranged by size: 1553 1052 926 901 714 262 255 197 SnaBI TAC'GTA Cuts at: 493 3651 493 Size: 3158 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5860 SphI G_CATG'C Cuts at: 1132 2128 2207 3218 1132 Size: 996 79 1011 3774 Fragments arranged by size: 3774 1011 996 79 SspI AAT'ATT Cuts at: 5 52 3784 5 Size: 47 3732 2081 Fragments arranged by size: 3732 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5860 StyI C'CwwG_G Cuts at: 513 1065 2004 2433 2640 2802 2838 3094 3299 Size: 552 939 429 207 162 36 256 205 Cuts at: 3299 513 Size: 3074 Fragments arranged by size: 3074 939 552 429 256 207 205 162 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 2365 2386 3887 3904 4057 4242 Size: 1255 588 197 21 1501 17 153 185 Cuts at: 4242 4687 325 Size: 445 1498 Fragments arranged by size: 1501 1498 1255 588 445 197 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4106 5818 96 Size: 274 80 33 51 1443 2129 1712 138 Fragments arranged by size: 2129 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5820 5852 5820 Size: 32 5828 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5860 XbaI T'CTAG_A Cuts at: 3196 3196 Size: 5860 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2446 2446 Size: 5860 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5860 XmaI C'CCGG_G Cuts at: 1870 3069 3185 1870 Size: 1199 116 4545 Fragments arranged by size: 4545 1199 116 XmnI GAAnn'nnTTC Cuts at: 3989 3989 Size: 5860 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2610 3668 276 Size: 53 83 186 936 1076 1058 2468 Fragments arranged by size: 2468 1076 1058 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI