Restriction map for
pFN21AE0468
(Linear) (Six-base) MAPSORT of: KIEE0468.seq Check: 1226 from: 1 to: 1155
KIEE0468.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 0 607 1155
Size: 607 548
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 660 793 1155
Size: 660 133 362
Fragments arranged by size:
660 362 133
AflIII A'CryG_T
Cuts at: 0 224 1155
Size: 224 931
AhdI GACnn_n'nnGTC
Cuts at: 0 73 1155
Size: 73 1082
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 599 1155
Size: 599 556
AlwNI CAG_nnn'CTG
Cuts at: 0 70 1155
Size: 70 1085
ApaI G_GGCC'C
Cuts at: 0 793 1064 1155
Size: 793 271 91
Fragments arranged by size:
793 271 91
ApaLI G'TGCA_C
Cuts at: 0 211 1155
Size: 211 944
ApoI r'AATT_y
Cuts at: 0 281 1155
Size: 281 874
AvaI C'yCGr_G
Cuts at: 0 976 1064 1155
Size: 976 88 91
Fragments arranged by size:
976 91 88
BamHI G'GATC_C
Cuts at: 0 32 1155
Size: 32 1123
BanI G'GyrC_C
Cuts at: 0 292 362 488 541 625 878 1093 1155
Size: 292 70 126 53 84 253 215 62
Fragments arranged by size:
292 253 215 126 84 70 62 53
BanII G_rGCy'C
Cuts at: 0 793 1064 1155
Size: 793 271 91
Fragments arranged by size:
793 271 91
BbeI G_GCGC'C
Cuts at: 0 366 492 629 882 1155
Size: 366 126 137 253 273
Fragments arranged by size:
366 273 253 137 126
BbsI GAAGACnn'nnnn_
Cuts at: 0 666 1155
Size: 666 489
BbvCI CC'TCA_GC
Cuts at: 0 1020 1155
Size: 1020 135
BclI T'GATC_A
Cuts at: 0 314 1155
Size: 314 841
BglII A'GATC_T
Cuts at: 0 386 1155
Size: 386 769
BlpI GC'TnA_GC
Cuts at: 0 846 1155
Size: 846 309
Bme1580I G_kGCm'C
Cuts at: 0 215 793 977 1064 1098 1117 1155
Size: 215 578 184 87 34 19 38
Fragments arranged by size:
578 215 184 87 38 34 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 964 1147 1155
Size: 964 183 8
Fragments arranged by size:
964 183 8
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 589 1062 1155
Size: 47 542 473 93
Fragments arranged by size:
542 473 93 47
Bpu10I CC'TnA_GC
Cuts at: 0 1020 1155
Size: 1020 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 928 1155
Size: 178 750 227
Fragments arranged by size:
750 227 178
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 598 625 1155
Size: 9 589 27 530
Fragments arranged by size:
589 530 27 9
BsaHI Gr'CG_yC
Cuts at: 0 363 489 604 626 879 1155
Size: 363 126 115 22 253 276
Fragments arranged by size:
363 276 253 126 115 22
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 502 532 913 943 1155
Size: 502 30 381 30 212
Fragments arranged by size:
502 381 212 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 472 475 1155
Size: 472 3 680
Fragments arranged by size:
680 472 3
BseYI C'CCAG_C
Cuts at: 0 156 346 792 809 1155
Size: 156 190 446 17 346
Fragments arranged by size:
446 346 190 156 17
BsiHKAI G_wGCw'C
Cuts at: 0 215 1155
Size: 215 940
BsmI GAATG_Cn'
Cuts at: 0 56 1155
Size: 56 1099
Bsp1286I G_dGCh'C
Cuts at: 0 215 793 977 1064 1098 1117 1155
Size: 215 578 184 87 34 19 38
Fragments arranged by size:
578 215 184 87 38 34 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 1155
Size: 78 1077
BsrFI r'CCGG_y
Cuts at: 0 52 1155
Size: 52 1103
BstAPI GCAn_nnn'nTGC
Cuts at: 0 424 1155
Size: 424 731
BstEII G'GTnAC_C
Cuts at: 0 996 1035 1155
Size: 996 39 120
Fragments arranged by size:
996 120 39
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 1155
Size: 411 744
BstYI r'GATC_y
Cuts at: 0 32 129 386 563 711 1155
Size: 32 97 257 177 148 444
Fragments arranged by size:
444 257 177 148 97 32
BtgI C'CryG_G
Cuts at: 0 167 288 1155
Size: 167 121 867
Fragments arranged by size:
867 167 121
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 168 1155
Size: 168 987
BtsI GCAGTG_nn'
Cuts at: 0 434 497 871 1155
Size: 434 63 374 284
Fragments arranged by size:
434 374 284 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 994 1155
Size: 994 161
EaeI y'GGCC_r
Cuts at: 0 17 1155
Size: 17 1138
EarI CTCTTCn'nnn_
Cuts at: 0 109 1155
Size: 109 1046
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 47 589 660 793 1062 1155
Size: 47 542 71 133 269 93
Fragments arranged by size:
542 269 133 93 71 47
EcoO109I rG'GnC_Cy
Cuts at: 0 67 768 789 1060 1061 1086 1148 1155
Size: 67 701 21 271 1 25 62 7
Fragments arranged by size:
701 271 67 62 25 21 7 1
HaeII r_GCGC'y
Cuts at: 0 366 492 629 882 889 1155
Size: 366 126 137 253 7 266
Fragments arranged by size:
366 266 253 137 126 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 59 91 913 945 1155
Size: 59 32 822 32 210
Fragments arranged by size:
822 210 59 32 32
KasI G'GCGC_C
Cuts at: 0 362 488 625 878 1155
Size: 362 126 137 253 277
Fragments arranged by size:
362 277 253 137 126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1155
Size: 428 727
MscI TGG'CCA
Cuts at: 0 19 1155
Size: 19 1136
MslI CAynn'nnrTG
Cuts at: 0 529 1155
Size: 529 626
MspA1I CmG'CkG
Cuts at: 0 964 1155
Size: 964 191
NaeI GCC'GGC
Cuts at: 0 54 1155
Size: 54 1101
NarI GG'CG_CC
Cuts at: 0 363 489 626 879 1155
Size: 363 126 137 253 276
Fragments arranged by size:
363 276 253 137 126
NgoMIV G'CCGG_C
Cuts at: 0 52 1155
Size: 52 1103
NspI r_CATG'y
Cuts at: 0 123 202 228 534 1155
Size: 123 79 26 306 621
Fragments arranged by size:
621 306 123 79 26
PciI A'CATG_T
Cuts at: 0 224 1155
Size: 224 931
PfoI T'CCnGG_A
Cuts at: 0 63 566 1155
Size: 63 503 589
Fragments arranged by size:
589 503 63
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 322 354 1155
Size: 322 32 801
Fragments arranged by size:
801 322 32
PpuMI rG'GwC_Cy
Cuts at: 0 67 768 1148 1155
Size: 67 701 380 7
Fragments arranged by size:
701 380 67 7
PspOMI G'GGCC_C
Cuts at: 0 789 1060 1155
Size: 789 271 95
Fragments arranged by size:
789 271 95
PstI C_TGCA'G
Cuts at: 0 259 441 1155
Size: 259 182 714
Fragments arranged by size:
714 259 182
PvuII CAG'CTG
Cuts at: 0 964 1155
Size: 964 191
SapI GCTCTTCn'nnn_
Cuts at: 0 109 1155
Size: 109 1046
SfcI C'TryA_G
Cuts at: 0 255 437 1155
Size: 255 182 718
Fragments arranged by size:
718 255 182
SfoI GGC'GCC
Cuts at: 0 364 490 627 880 1155
Size: 364 126 137 253 275
Fragments arranged by size:
364 275 253 137 126
SmaI CCC'GGG
Cuts at: 0 1066 1155
Size: 1066 89
SmlI C'TyrA_G
Cuts at: 0 193 907 1155
Size: 193 714 248
Fragments arranged by size:
714 248 193
SphI G_CATG'C
Cuts at: 0 123 202 1155
Size: 123 79 953
Fragments arranged by size:
953 123 79
StyI C'CwwG_G
Cuts at: 0 428 635 797 833 1089 1155
Size: 428 207 162 36 256 66
Fragments arranged by size:
428 256 207 162 66 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 163 360 381 1155
Size: 163 197 21 774
Fragments arranged by size:
774 197 163 21
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 441 1155
Size: 441 714
XmaI C'CCGG_G
Cuts at: 0 1064 1155
Size: 1064 91
ZraI GAC'GTC
Cuts at: 0 605 1155
Size: 605 550
Enzymes that do cut:
AatII AcuI AflIII AhdI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BclI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I
BspMI BsrFI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI DrdI EaeI EarI Eco57MI EcoO109I HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PciI PfoI PpiI PpuMI PspOMI PstI PvuII
SapI SfcI SfoI SmaI SmlI SphI StyI TaqII
XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BfrBI BglI BmgBI BmtI BplI BsaAI BsaBI BsaWI
BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I ClaI CspCI
DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PflMI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmnI