################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AviII BbeI BbrPI BciVI BclI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BseRI BsiEI BsiWI BsmBI BspDI BspHI BssSI BstBI BstXI CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI FbaI FseI FspI HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 142 GACGTC GACGT^C AccIII 128 TCCGGA T^CCGGA AcsI 2346 GAATTT [GA]^AATT[CT] AcyI 142 GACGTC G[GA]^CG[CT]C AlwNI 817 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 504 CCTGAGG CC^T[ACGT]AGG ApoI 2346 GAATTT [GA]^AATT[CT] Asp700I 1190 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 645 CCTAGG C^CTAGG BanII 2671 GAGCTC G[GA]GC[CT]^C BbvCI 9 GCTGAGG CCTCAGC(-5/-2) BcgI 1717 CGAACCGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 2256 AGATCT A^GATCT BlnI 645 CCTAGG C^CTAGG BsaHI 142 GACGTC G[GA]^CG[CT]C BsaI 480 GGTCTC GGTCTC(1/5) BseAI 128 TCCGGA T^CCGGA BsmAI 481 GTCTC GTCTC(1/5) BsmI 2288 GCATTC GAATGC(1/-1) BspEI 128 TCCGGA T^CCGGA BspMI 733 GCAGGT ACCTGC(4/8) BsrBI 2432 CCGCTC CCGCTC(-3/-3) Bst1107I 2702 GTATAC GTA^TAC BstAPI 1240 GCAGTCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2702 GTATAC GTA^TAC Bsu36I 504 CCTGAGG CC^T[ACGT]AGG EarI 511 CTCTTC CTCTTC(1/4) Eco81I 504 CCTGAGG CC^T[ACGT]AGG EcoRV 982 GATATC GAT^ATC EcoT22I 536 ATGCAT ATGCA^T Hin1I 142 GACGTC G[GA]^CG[CT]C HincII 1125 GTTGAC GT[CT]^[GA]AC HindII 1125 GTTGAC GT[CT]^[GA]AC HindIII 2583 AAGCTT A^AGCTT Ksp632I 511 CTCTTC CTCTTC(1/4) MroI 128 TCCGGA T^CCGGA NcoI 623 CCATGG C^CATGG NsiI 536 ATGCAT ATGCA^T PflFI 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 180 GGGTCCC [GA]G^G[AT]CC[CT] SacI 2671 GAGCTC GAGCT^C SapI 510 GCTCTTC GCTCTTC(1/4) SmaI 1467 CCCGGG CCC^GGG SmlI 2239 CTCAAG C^T[CT][GA]AG Tth111I 1237 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1467 CCCGGG C^CCGGG XmaI 1467 CCCGGG C^CCGGG XmnI 1190 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 2305 ACATGT A^C[GA][CT]GT 2389 ACATGT Alw44I 169 GTGCAC G^TGCAC 1566 GTGCAC ApaLI 169 GTGCAC G^TGCAC 1566 GTGCAC BalI 915 TGGCCA TGG^CCA 1826 TGGCCA BfaI 646 CTAG C^TAG 2143 CTAG BsaWI 128 TCCGGA [AT]^CCGG[AT] 1720 ACCGGA BsgI 405 CTGCAC GTGCAG(16/14) 967 CTGCAC BspLU11I 2305 ACATGT A^CATGT 2389 ACATGT BsrFI 423 GCCGGT [GA]^CCGG[CT] 566 ACCGGC BstEII 1471 GGTTACC G^GT[ACGT]ACC 1648 GGTTACC BstPI 1471 GGTTACC G^GT[ACGT]ACC 1648 GGTTACC Cfr10I 423 GCCGGT [GA]^CCGG[CT] 566 ACCGGC DraII 180 GGGTCCC [GA]G^G[ACGT]CC[CT] 641 AGGCCCT DsaI 623 CCATGG C^C[GA][CT]GG 1022 CCACGG EcoO109I 180 GGGTCCC [GA]G^G[ACGT]CC[CT] 641 AGGCCCT EcoO65I 1471 GGTTACC G^GT[ACGT]ACC 1648 GGTTACC MaeI 646 CTAG C^TAG 2143 CTAG MluNI 915 TGGCCA TGG^CCA 1826 TGGCCA MscI 915 TGGCCA TGG^CCA 1826 TGGCCA PflMI 228 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 618 CCAGGCCATGG PvuII 7 CAGCTG CAG^CTG 942 CAGCTG Van91I 228 CCACAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 618 CCAGGCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 91 enzymes) # ######################################### AccI AccII AciI AfaI AluI AlwI AspHI AvaI AvaII BamHI BanI BbsI BbvI BcnI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII