(Circular) (Six-base) MAPSORT of: pFN21AE2103.seq Check: 2382 from: 1 to: 7471 pFN21AE2103 7471 bp With 182 enzymes: SgfI * March 28, 2012 12:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2729 2729 Size: 7471 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2151 5281 278 Size: 53 83 186 936 615 3130 2468 Fragments arranged by size: 3130 2468 936 615 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 2669 4692 4708 4814 1937 Size: 697 35 2023 16 106 4594 Fragments arranged by size: 4594 2023 697 106 35 16 Acc65I G'GTAC_C Cuts at: 4792 4792 Size: 7471 AclI AA'CG_TT Cuts at: 1563 5598 5971 1563 Size: 4035 373 3063 Fragments arranged by size: 4035 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3371 3494 5534 6918 7186 1632 Size: 1739 123 2040 1384 268 1917 Fragments arranged by size: 2040 1917 1739 1384 268 123 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7274 AflIII A'CryG_T Cuts at: 1203 4310 4394 6370 7347 1203 Size: 3107 84 1976 977 1327 Fragments arranged by size: 3107 1976 1327 977 84 AgeI A'CCGG_T Cuts at: 6302 6302 Size: 7471 AhdI GACnn_n'nnGTC Cuts at: 6200 6200 Size: 7471 AleI CACnn'nnGTG Cuts at: 1166 3395 1166 Size: 2229 5242 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2907 3362 1184 Size: 1723 455 5293 Fragments arranged by size: 5293 1723 455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4554 4586 4554 Size: 32 7439 AlwNI CAG_nnn'CTG Cuts at: 2827 5214 6786 2827 Size: 2387 1572 3512 Fragments arranged by size: 3512 2387 1572 ApaLI G'TGCA_C Cuts at: 2174 3571 5528 6684 2174 Size: 1397 1957 1156 2961 Fragments arranged by size: 2961 1957 1397 1156 ApoI r'AATT_y Cuts at: 1493 4351 4780 5006 5158 7267 1493 Size: 2858 429 226 152 2109 1697 Fragments arranged by size: 2858 2109 1697 429 226 152 AseI AT'TA_AT Cuts at: 160 1032 6025 160 Size: 872 4993 1606 Fragments arranged by size: 4993 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2555 3472 4335 4346 4796 1870 Size: 73 213 399 917 863 11 450 4545 Fragments arranged by size: 4545 917 863 450 399 213 73 11 AvrII C'CTAG_G Cuts at: 2650 2650 Size: 7471 BamHI G'GATC_C Cuts at: 2229 2316 2954 4801 5168 2229 Size: 87 638 1847 367 4532 Fragments arranged by size: 4532 1847 638 367 87 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2551 2599 4433 4792 6247 Size: 325 217 627 764 48 1834 359 1455 Cuts at: 6247 618 Size: 1842 Fragments arranged by size: 1842 1834 1455 764 627 359 325 217 48 BanII G_rGCy'C Cuts at: 1391 4680 4790 1391 Size: 3289 110 4072 Fragments arranged by size: 4072 3289 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3162 3531 3611 3781 928 Size: 971 1263 369 80 170 4618 Fragments arranged by size: 4618 1263 971 369 170 80 BbvCI CC'TCA_GC Cuts at: 2015 2015 Size: 7471 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3711 3745 5662 5696 1903 Size: 34 1774 34 1917 34 3678 Fragments arranged by size: 3678 1917 1774 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5363 6573 5363 Size: 1210 6261 BclI T'GATC_A Cuts at: 1548 1548 Size: 7471 BfrBI ATG'CAT Cuts at: 2543 2543 Size: 7471 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6082 136 Size: 107 122 71 631 605 4410 1525 Fragments arranged by size: 4410 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4261 7466 4261 Size: 3205 4266 BlpI GC'TnA_GC Cuts at: 4888 4888 Size: 7471 Bme1580I G_kGCm'C Cuts at: 1790 2178 2202 3575 5532 6688 1790 Size: 388 24 1373 1957 1156 2573 Fragments arranged by size: 2573 1957 1373 1156 388 24 BmgBI CAC'GTC Cuts at: 1318 2404 1318 Size: 1086 6385 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2752 3115 4638 Size: 416 53 83 399 24 1335 363 1523 Cuts at: 4638 5202 6160 442 Size: 564 958 1753 Fragments arranged by size: 1753 1523 1335 958 564 416 399 363 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7471 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3781 4259 3781 Size: 478 6993 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2827 3354 3378 3776 6131 1239 Size: 848 740 527 24 398 2355 2579 Fragments arranged by size: 2579 2355 848 740 527 398 24 Bpu10I CC'TnA_GC Cuts at: 2015 4224 2015 Size: 2209 5262 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4229 5596 6461 6759 4229 Size: 1367 865 298 4941 Fragments arranged by size: 4941 1367 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2491 6134 882 Size: 617 992 3643 2219 Fragments arranged by size: 3643 2219 992 617 BsaAI yAC'GTr Cuts at: 493 1204 5262 493 Size: 711 4058 2702 Fragments arranged by size: 4058 2702 711 BsaBI GATnn'nnATC Cuts at: 7465 7465 Size: 7471 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2148 5278 5660 6295 Size: 53 83 186 936 615 3130 382 635 Cuts at: 6295 275 Size: 1451 Fragments arranged by size: 3130 1451 936 635 615 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2133 3725 5904 6302 6576 6723 7134 2133 Size: 1592 2179 398 274 147 411 2470 Fragments arranged by size: 2470 2179 1592 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7246 Fragments arranged by size: 7246 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7471 BseYI C'CCAG_C Cuts at: 2189 2305 2949 4513 6674 7170 2189 Size: 116 644 1564 2161 496 2490 Fragments arranged by size: 2490 2161 1564 644 496 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2395 2957 1163 Size: 573 659 562 5677 Fragments arranged by size: 5677 659 573 562 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4921 5682 6710 1540 Size: 275 186 2920 761 1028 2301 Fragments arranged by size: 2920 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2178 3575 4680 5532 5617 6688 2076 Size: 102 1397 1105 852 85 1071 2859 Fragments arranged by size: 2859 1397 1105 1071 852 102 85 BsmI GAATG_Cn' Cuts at: 4293 4982 5075 4293 Size: 689 93 6689 Fragments arranged by size: 6689 689 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2178 2202 3575 4680 4790 5532 Size: 399 286 102 24 1373 1105 110 742 Cuts at: 5532 5617 6688 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1373 1105 1071 742 399 286 110 102 85 24 BspEI T'CCGG_A Cuts at: 2133 7134 2133 Size: 5001 2470 BspHI T'CATG_A Cuts at: 5360 7090 5360 Size: 1730 5741 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2729 4826 844 Size: 1885 2097 3489 Fragments arranged by size: 3489 2097 1885 BsrBI CCG'CTC Cuts at: 1394 1823 4439 5358 1394 Size: 429 2616 919 3507 Fragments arranged by size: 3507 2616 919 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2751 3370 3688 3804 3810 5966 6140 Size: 1183 1503 619 318 116 6 2156 174 Cuts at: 6140 65 Size: 1396 Fragments arranged by size: 2156 1503 1396 1183 619 318 174 116 6 BsrFI r'CCGG_y Cuts at: 1700 1810 2428 2571 6115 6302 1700 Size: 110 618 143 3544 187 2869 Fragments arranged by size: 3544 2869 618 187 143 110 BsrGI T'GTAC_A Cuts at: 96 4386 4539 4729 96 Size: 4290 153 190 2838 Fragments arranged by size: 4290 2838 190 153 BssHII G'CGCG_C Cuts at: 1925 2082 2099 2310 1925 Size: 157 17 211 7086 Fragments arranged by size: 7086 211 157 17 BssSI C'ACGA_G Cuts at: 5531 6543 5531 Size: 1012 6459 BstAPI GCAn_nnn'nTGC Cuts at: 794 3251 794 Size: 2457 5014 BstBI TT'CG_AA Cuts at: 6275 6275 Size: 7471 BstEII G'GTnAC_C Cuts at: 1184 3476 3653 1184 Size: 2292 177 5002 Fragments arranged by size: 5002 2292 177 BstYI r'GATC_y Cuts at: 1972 2229 2316 2523 2886 2954 3286 4261 4801 Size: 257 87 207 363 68 332 975 540 Cuts at: 4801 5168 5553 5570 7022 7108 7120 7466 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 975 540 385 367 363 346 332 257 207 87 86 68 17 12 BstZ17I GTA'TAC Cuts at: 4709 4709 Size: 7471 Bsu36I CC'TnA_GG Cuts at: 2510 2510 Size: 7471 BtgI C'CryG_G Cuts at: 513 1065 2004 2628 3027 513 Size: 552 939 624 399 4957 Fragments arranged by size: 4957 939 624 552 399 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2261 3663 487 Size: 683 56 1035 1402 4295 Fragments arranged by size: 4295 1402 1035 683 56 BtsI GCAGTG_nn' Cuts at: 2969 3500 3539 3969 4987 5781 5801 2969 Size: 531 39 430 1018 794 20 4639 Fragments arranged by size: 4639 1018 794 531 430 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7471 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3805 3840 529 Size: 35 3241 35 4160 Fragments arranged by size: 4160 3241 35 35 DraI TTT'AAA Cuts at: 4775 4775 Size: 7471 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6478 809 Size: 507 5162 1802 Fragments arranged by size: 5162 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2104 2920 3831 Size: 54 1425 50 275 18 274 816 911 Cuts at: 3831 4918 5807 7436 8 Size: 1087 889 1629 43 Fragments arranged by size: 1629 1425 1087 911 889 816 275 274 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4918 1537 Size: 275 3106 4090 Fragments arranged by size: 4090 3106 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2522 5401 1353 Size: 1169 2879 3423 Fragments arranged by size: 3423 2879 1169 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6058 6432 6578 387 Size: 1064 138 4469 374 146 1280 Fragments arranged by size: 4469 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4678 4678 Size: 7471 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2827 3354 3371 3378 3494 3776 Size: 393 455 740 527 17 7 116 282 Cuts at: 3776 5534 6131 6918 7186 1239 Size: 1758 597 787 268 1524 Fragments arranged by size: 1758 1524 787 740 597 527 455 393 282 268 116 17 7 EcoO109I rG'GnC_Cy Cuts at: 2186 2647 2186 Size: 461 7010 EcoRI G'AATT_C Cuts at: 4780 5158 4780 Size: 378 7093 EcoRV GAT'ATC Cuts at: 2989 2989 Size: 7471 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4606 4606 Size: 7471 FspI TGC'GCA Cuts at: 5977 5977 Size: 7471 HaeII r_GCGC'y Cuts at: 1678 2381 2578 4514 6618 1678 Size: 703 197 1936 2104 2531 Fragments arranged by size: 2531 2104 1936 703 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2573 2605 6112 6144 6186 Size: 32 135 32 1001 32 3507 32 42 Cuts at: 6186 6218 1373 Size: 32 2626 Fragments arranged by size: 3507 2626 1001 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 4815 5061 669 Size: 988 281 1194 1683 246 3079 Fragments arranged by size: 3079 1683 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 4588 748 Size: 3840 3631 HpaI GTT'AAC Cuts at: 5061 5061 Size: 7471 KpnI G_GTAC'C Cuts at: 4796 4796 Size: 7471 MfeI C'AATT_G Cuts at: 5070 5070 Size: 7471 MluI A'CGCG_T Cuts at: 7347 7347 Size: 7471 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2424 2437 3228 3611 6585 6769 1305 Size: 1119 13 791 383 2974 184 2007 Fragments arranged by size: 2974 2007 1119 791 383 184 13 MscI TGG'CCA Cuts at: 10 64 1489 1832 2922 3833 10 Size: 54 1425 343 1090 911 3648 Fragments arranged by size: 3648 1425 1090 911 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2979 3395 3580 5429 5788 5947 518 Size: 648 1813 416 185 1849 359 159 2042 Fragments arranged by size: 2042 1849 1813 648 416 359 185 159 MspA1I CmG'CkG Cuts at: 1606 2014 2193 2949 4448 4887 5564 6712 6957 Size: 408 179 756 1499 439 677 1148 245 Cuts at: 6957 1606 Size: 2120 Fragments arranged by size: 2120 1499 1148 756 677 439 408 245 179 NcoI C'CATG_G Cuts at: 513 1065 2004 2628 513 Size: 552 939 624 5356 Fragments arranged by size: 5356 939 624 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7471 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7471 NotI GC'GGCC_GC Cuts at: 4918 4918 Size: 7471 NsiI A_TGCA'T Cuts at: 2545 2545 Size: 7471 NspI r_CATG'y Cuts at: 1132 1229 3532 3684 4314 4398 4531 4829 6374 Size: 97 2303 152 630 84 133 298 1545 Cuts at: 6374 1132 Size: 2229 Fragments arranged by size: 2303 2229 1545 630 298 152 133 97 84 PasI CC'CwG_GG Cuts at: 1348 3771 1348 Size: 2423 5048 PciI A'CATG_T Cuts at: 4310 4394 6370 4310 Size: 84 1976 5411 Fragments arranged by size: 5411 1976 84 PflMI CCAn_nnn'nTGG Cuts at: 1108 2239 2629 1108 Size: 1131 390 5950 Fragments arranged by size: 5950 1131 390 PfoI T'CCnGG_A Cuts at: 2319 2826 2319 Size: 507 6964 PmeI GTTT'AAAC Cuts at: 4775 4775 Size: 7471 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3498 3530 5540 5572 7053 7085 3498 Size: 32 2010 32 1481 32 3884 Fragments arranged by size: 3884 2010 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2186 2186 Size: 7471 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7471 PsiI TTA'TAA Cuts at: 5041 5041 Size: 7471 PstI C_TGCA'G Cuts at: 830 4823 830 Size: 3993 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7471 PvuII CAG'CTG Cuts at: 2014 2949 2014 Size: 935 6536 SacI G_AGCT'C Cuts at: 4680 4680 Size: 7471 SalI G'TCGA_C Cuts at: 1936 4813 1936 Size: 2877 4594 SanDI GG'GwC_CC Cuts at: 2186 2186 Size: 7471 SapI GCTCTTCn'nnn_ Cuts at: 2522 2522 Size: 7471 SbfI CC_TGCA'GG Cuts at: 4823 4823 Size: 7471 ScaI AGT'ACT Cuts at: 5719 7431 5719 Size: 1712 5759 SfcI C'TryA_G Cuts at: 826 1045 4819 5954 6635 6826 826 Size: 219 3774 1135 681 191 1471 Fragments arranged by size: 3774 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7471 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7471 SmaI CCC'GGG Cuts at: 1872 3474 4798 1872 Size: 1602 1324 4545 Fragments arranged by size: 4545 1602 1324 SmlI C'TyrA_G Cuts at: 820 1017 1943 4244 5575 6476 6738 820 Size: 197 926 2301 1331 901 262 1553 Fragments arranged by size: 2301 1553 1331 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5262 493 Size: 4769 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7471 SphI G_CATG'C Cuts at: 1132 4829 1132 Size: 3697 3774 SspI AAT'ATT Cuts at: 5 52 5395 5 Size: 47 5343 2081 Fragments arranged by size: 5343 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7471 StyI C'CwwG_G Cuts at: 513 1065 2004 2181 2628 2650 4281 4910 513 Size: 552 939 177 447 22 1631 629 3074 Fragments arranged by size: 3074 1631 939 629 552 447 177 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2473 2923 4195 4688 5498 5515 5668 Size: 1255 893 450 1272 493 810 17 153 Cuts at: 5668 5853 6298 325 Size: 185 445 1498 Fragments arranged by size: 1498 1272 1255 893 810 493 450 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4313 4386 4539 Size: 274 80 33 51 1443 2336 73 153 Cuts at: 4539 4729 5717 7429 96 Size: 190 988 1712 138 Fragments arranged by size: 2336 1712 1443 988 274 190 153 138 80 73 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7431 7463 7431 Size: 32 7439 Tth111I GACn'n_nGTC Cuts at: 1316 3245 1316 Size: 1929 5542 XbaI T'CTAG_A Cuts at: 4807 4807 Size: 7471 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2302 3751 3826 3955 2302 Size: 1449 75 129 5818 Fragments arranged by size: 5818 1449 129 75 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7471 XmaI C'CCGG_G Cuts at: 1870 3472 4796 1870 Size: 1602 1324 4545 Fragments arranged by size: 4545 1602 1324 XmnI GAAnn'nnTTC Cuts at: 3199 5600 3199 Size: 2401 5070 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2149 5279 276 Size: 53 83 186 936 615 3130 2468 Fragments arranged by size: 3130 2468 936 615 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BsiWI BsmBI BstXI DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI