(Linear) (Six-base) MAPSORT of: KIEE2103.seq Check: 4490 from: 1 to: 2766 KIEE2103.orf With 182 enzymes: SgfI * March 22, 2012 15:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 724 2766 Size: 724 2042 AatII G_ACGT'C Cuts at: 0 146 2766 Size: 146 2620 AccI GT'mk_AC Cuts at: 0 629 664 2687 2703 2766 Size: 629 35 2023 16 63 Fragments arranged by size: 2023 629 63 35 16 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1366 1489 2766 Size: 1366 123 1277 Fragments arranged by size: 1366 1277 123 AflIII A'CryG_T Cuts at: 0 2305 2389 2766 Size: 2305 84 377 Fragments arranged by size: 2305 377 84 AleI CACnn'nnGTG Cuts at: 0 1390 2766 Size: 1390 1376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 902 1357 2766 Size: 902 455 1409 Fragments arranged by size: 1409 902 455 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2549 2581 2766 Size: 2549 32 185 Fragments arranged by size: 2549 185 32 AlwNI CAG_nnn'CTG Cuts at: 0 822 2766 Size: 822 1944 ApaLI G'TGCA_C Cuts at: 0 169 1566 2766 Size: 169 1397 1200 Fragments arranged by size: 1397 1200 169 ApoI r'AATT_y Cuts at: 0 2346 2766 Size: 2346 420 AvaI C'yCGr_G Cuts at: 0 151 550 1467 2330 2341 2766 Size: 151 399 917 863 11 425 Fragments arranged by size: 917 863 425 399 151 11 AvrII C'CTAG_G Cuts at: 0 645 2766 Size: 645 2121 BamHI G'GATC_C Cuts at: 0 224 311 949 2766 Size: 224 87 638 1817 Fragments arranged by size: 1817 638 224 87 BanI G'GyrC_C Cuts at: 0 546 594 2428 2766 Size: 546 48 1834 338 Fragments arranged by size: 1834 546 338 48 BanII G_rGCy'C Cuts at: 0 2675 2766 Size: 2675 91 BbsI GAAGACnn'nnnn_ Cuts at: 0 1157 1526 1606 1776 2766 Size: 1157 369 80 170 990 Fragments arranged by size: 1157 990 369 170 80 BbvCI CC'TCA_GC Cuts at: 0 10 2766 Size: 10 2756 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1706 1740 2766 Size: 1706 34 1026 Fragments arranged by size: 1706 1026 34 BfrBI ATG'CAT Cuts at: 0 538 2766 Size: 538 2228 BglII A'GATC_T Cuts at: 0 2256 2766 Size: 2256 510 Bme1580I G_kGCm'C Cuts at: 0 173 197 1570 2766 Size: 173 24 1373 1196 Fragments arranged by size: 1373 1196 173 24 BmgBI CAC'GTC Cuts at: 0 399 2766 Size: 399 2367 BmrI ACTGGGnnnn_n' Cuts at: 0 747 1110 2633 2766 Size: 747 363 1523 133 Fragments arranged by size: 1523 747 363 133 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1776 2254 2766 Size: 1776 478 512 Fragments arranged by size: 1776 512 478 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 822 1349 1373 1771 2766 Size: 82 740 527 24 398 995 Fragments arranged by size: 995 740 527 398 82 24 Bpu10I CC'TnA_GC Cuts at: 0 10 2219 2766 Size: 10 2209 547 Fragments arranged by size: 2209 547 10 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2224 2766 Size: 2224 542 BsaI GGTCTCn'nnnn_ Cuts at: 0 486 2766 Size: 486 2280 BsaHI Gr'CG_yC Cuts at: 0 143 2766 Size: 143 2623 BsaWI w'CCGG_w Cuts at: 0 128 1720 2766 Size: 128 1592 1046 Fragments arranged by size: 1592 1046 128 BseYI C'CCAG_C Cuts at: 0 184 300 944 2508 2766 Size: 184 116 644 1564 258 Fragments arranged by size: 1564 644 258 184 116 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 390 952 2766 Size: 390 562 1814 Fragments arranged by size: 1814 562 390 BsiHKAI G_wGCw'C Cuts at: 0 71 173 1570 2675 2766 Size: 71 102 1397 1105 91 Fragments arranged by size: 1397 1105 102 91 71 BsmI GAATG_Cn' Cuts at: 0 2288 2766 Size: 2288 478 Bsp1286I G_dGCh'C Cuts at: 0 71 173 197 1570 2675 2766 Size: 71 102 24 1373 1105 91 Fragments arranged by size: 1373 1105 102 91 71 24 BspEI T'CCGG_A Cuts at: 0 128 2766 Size: 128 2638 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 724 2766 Size: 724 2042 BsrBI CCG'CTC Cuts at: 0 2434 2766 Size: 2434 332 BsrDI GCAATG_nn' Cuts at: 0 746 1365 1683 1799 1805 2766 Size: 746 619 318 116 6 961 Fragments arranged by size: 961 746 619 318 116 6 BsrFI r'CCGG_y Cuts at: 0 423 566 2766 Size: 423 143 2200 Fragments arranged by size: 2200 423 143 BsrGI T'GTAC_A Cuts at: 0 2381 2534 2724 2766 Size: 2381 153 190 42 Fragments arranged by size: 2381 190 153 42 BssHII G'CGCG_C Cuts at: 0 77 94 305 2766 Size: 77 17 211 2461 Fragments arranged by size: 2461 211 77 17 BstAPI GCAn_nnn'nTGC Cuts at: 0 1246 2766 Size: 1246 1520 BstEII G'GTnAC_C Cuts at: 0 1471 1648 2766 Size: 1471 177 1118 Fragments arranged by size: 1471 1118 177 BstYI r'GATC_y Cuts at: 0 224 311 518 881 949 1281 2256 2766 Size: 224 87 207 363 68 332 975 510 Fragments arranged by size: 975 510 363 332 224 207 87 68 BstZ17I GTA'TAC Cuts at: 0 2704 2766 Size: 2704 62 Bsu36I CC'TnA_GG Cuts at: 0 505 2766 Size: 505 2261 BtgI C'CryG_G Cuts at: 0 623 1022 2766 Size: 623 399 1744 Fragments arranged by size: 1744 623 399 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 256 1658 2766 Size: 256 1402 1108 Fragments arranged by size: 1402 1108 256 BtsI GCAGTG_nn' Cuts at: 0 964 1495 1534 1964 2766 Size: 964 531 39 430 802 Fragments arranged by size: 964 802 531 430 39 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1800 1835 2766 Size: 1800 35 931 Fragments arranged by size: 1800 931 35 EaeI y'GGCC_r Cuts at: 0 99 915 1826 2766 Size: 99 816 911 940 Fragments arranged by size: 940 911 816 99 EarI CTCTTCn'nnn_ Cuts at: 0 517 2766 Size: 517 2249 EcoICRI GAG'CTC Cuts at: 0 2673 2766 Size: 2673 93 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 822 1349 1366 1373 1489 1771 2766 Size: 82 740 527 17 7 116 282 995 Fragments arranged by size: 995 740 527 282 116 82 17 7 EcoO109I rG'GnC_Cy Cuts at: 0 181 642 2766 Size: 181 461 2124 Fragments arranged by size: 2124 461 181 EcoRV GAT'ATC Cuts at: 0 984 2766 Size: 984 1782 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2601 2766 Size: 2601 165 HaeII r_GCGC'y Cuts at: 0 376 573 2509 2766 Size: 376 197 1936 257 Fragments arranged by size: 1936 376 257 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 568 600 2766 Size: 568 32 2166 Fragments arranged by size: 2166 568 32 HincII GTy'rAC Cuts at: 0 1127 2766 Size: 1127 1639 HindIII A'AGCT_T Cuts at: 0 2583 2766 Size: 2583 183 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 419 432 1223 1606 2766 Size: 419 13 791 383 1160 Fragments arranged by size: 1160 791 419 383 13 MscI TGG'CCA Cuts at: 0 917 1828 2766 Size: 917 911 938 Fragments arranged by size: 938 917 911 MslI CAynn'nnrTG Cuts at: 0 974 1390 1575 2766 Size: 974 416 185 1191 Fragments arranged by size: 1191 974 416 185 MspA1I CmG'CkG Cuts at: 0 9 188 944 2443 2766 Size: 9 179 756 1499 323 Fragments arranged by size: 1499 756 323 179 9 NcoI C'CATG_G Cuts at: 0 623 2766 Size: 623 2143 NsiI A_TGCA'T Cuts at: 0 540 2766 Size: 540 2226 NspI r_CATG'y Cuts at: 0 1527 1679 2309 2393 2526 2766 Size: 1527 152 630 84 133 240 Fragments arranged by size: 1527 630 240 152 133 84 PasI CC'CwG_GG Cuts at: 0 1766 2766 Size: 1766 1000 PciI A'CATG_T Cuts at: 0 2305 2389 2766 Size: 2305 84 377 Fragments arranged by size: 2305 377 84 PflMI CCAn_nnn'nTGG Cuts at: 0 234 624 2766 Size: 234 390 2142 Fragments arranged by size: 2142 390 234 PfoI T'CCnGG_A Cuts at: 0 314 821 2766 Size: 314 507 1945 Fragments arranged by size: 1945 507 314 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1493 1525 2766 Size: 1493 32 1241 Fragments arranged by size: 1493 1241 32 PpuMI rG'GwC_Cy Cuts at: 0 181 2766 Size: 181 2585 PvuII CAG'CTG Cuts at: 0 9 944 2766 Size: 9 935 1822 Fragments arranged by size: 1822 935 9 SacI G_AGCT'C Cuts at: 0 2675 2766 Size: 2675 91 SanDI GG'GwC_CC Cuts at: 0 181 2766 Size: 181 2585 SapI GCTCTTCn'nnn_ Cuts at: 0 517 2766 Size: 517 2249 SmaI CCC'GGG Cuts at: 0 1469 2766 Size: 1469 1297 SmlI C'TyrA_G Cuts at: 0 2239 2766 Size: 2239 527 StyI C'CwwG_G Cuts at: 0 176 623 645 2276 2766 Size: 176 447 22 1631 490 Fragments arranged by size: 1631 490 447 176 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 468 918 2190 2683 2766 Size: 468 450 1272 493 83 Fragments arranged by size: 1272 493 468 450 83 TatI w'GTAC_w Cuts at: 0 2308 2381 2534 2724 2766 Size: 2308 73 153 190 42 Fragments arranged by size: 2308 190 153 73 42 Tth111I GACn'n_nGTC Cuts at: 0 1240 2766 Size: 1240 1526 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 297 1746 1821 1950 2766 Size: 297 1449 75 129 816 Fragments arranged by size: 1449 816 297 129 75 XmaI C'CCGG_G Cuts at: 0 1467 2766 Size: 1467 1299 XmnI GAAnn'nnTTC Cuts at: 0 1194 2766 Size: 1194 1572 ZraI GAC'GTC Cuts at: 0 144 2766 Size: 144 2622 Enzymes that do cut: AarI AatII AccI AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BfrBI BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PpiI PpuMI PvuII SacI SanDI SapI SmaI SmlI StyI TaqII TatI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: Acc65I AclI AfeI AflII AgeI AhdI ApaI AscI AseI AsiSI BaeI BbeI BciVI BclI BglI BlpI BmtI BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmBI BspHI BssSI BstBI BstXI ClaI DraI DraIII DrdI EagI EciI EcoNI EcoRI FseI FspI FspAI HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI