Restriction map for
pFN21AE0465
(Linear) (Six-base) MAPSORT of: KIEE0465.seq Check: 1623 from: 1 to: 657
KIEE0465.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AleI CACnn'nnGTG
Cuts at: 0 269 657
Size: 269 388
AlwNI CAG_nnn'CTG
Cuts at: 0 361 657
Size: 361 296
ApaI G_GGCC'C
Cuts at: 0 456 657
Size: 456 201
ApoI r'AATT_y
Cuts at: 0 135 657
Size: 135 522
AvrII C'CTAG_G
Cuts at: 0 316 657
Size: 316 341
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 205 238 657
Size: 205 33 419
Fragments arranged by size:
419 205 33
BanI G'GyrC_C
Cuts at: 0 483 513 543 657
Size: 483 30 30 114
Fragments arranged by size:
483 114 30 30
BanII G_rGCy'C
Cuts at: 0 456 657
Size: 456 201
BciVI GTATCCnnnnn_n'
Cuts at: 0 233 657
Size: 233 424
BlpI GC'TnA_GC
Cuts at: 0 392 437 467 497 527 587 657
Size: 392 45 30 30 30 60 70
Fragments arranged by size:
392 70 60 45 30 30 30
Bme1580I G_kGCm'C
Cuts at: 0 456 657
Size: 456 201
BmrI ACTGGGnnnn_n'
Cuts at: 0 157 173 657
Size: 157 16 484
Fragments arranged by size:
484 157 16
Bpu10I CC'TnA_GC
Cuts at: 0 282 657
Size: 282 375
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 137 296 657
Size: 137 159 361
Fragments arranged by size:
361 159 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 657
Size: 392 265
BsmBI CGTCTCn'nnnn_
Cuts at: 0 45 657
Size: 45 612
Bsp1286I G_dGCh'C
Cuts at: 0 456 657
Size: 456 201
BstAPI GCAn_nnn'nTGC
Cuts at: 0 373 520 657
Size: 373 147 137
Fragments arranged by size:
373 147 137
BstEII G'GTnAC_C
Cuts at: 0 91 212 657
Size: 91 121 445
Fragments arranged by size:
445 121 91
BtgI C'CryG_G
Cuts at: 0 270 657
Size: 270 387
EarI CTCTTCn'nnn_
Cuts at: 0 43 657
Size: 43 614
EcoNI CCTnn'n_nnAGG
Cuts at: 0 446 657
Size: 446 211
EcoO109I rG'GnC_Cy
Cuts at: 0 452 453 657
Size: 452 1 204
Fragments arranged by size:
452 204 1
HindIII A'AGCT_T
Cuts at: 0 4 657
Size: 4 653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 531 657
Size: 471 60 126
Fragments arranged by size:
471 126 60
MslI CAynn'nnrTG
Cuts at: 0 269 657
Size: 269 388
MspA1I CmG'CkG
Cuts at: 0 361 373 391 406 436 466 526 565
Size: 361 12 18 15 30 30 60 39
Cuts at: 565 586 657
Size: 21 71
Fragments arranged by size:
361 71 60 39 30 30 21 18
15 12
PasI CC'CwG_GG
Cuts at: 0 187 657
Size: 187 470
PfoI T'CCnGG_A
Cuts at: 0 243 657
Size: 243 414
PshAI GACnn'nnGTC
Cuts at: 0 89 657
Size: 89 568
PspOMI G'GGCC_C
Cuts at: 0 452 657
Size: 452 205
PstI C_TGCA'G
Cuts at: 0 366 405 450 480 510 540 570 657
Size: 366 39 45 30 30 30 30 87
Fragments arranged by size:
366 87 45 39 30 30 30 30
PvuII CAG'CTG
Cuts at: 0 361 391 406 436 466 526 565 586
Size: 361 30 15 30 30 60 39 21
Cuts at: 586 657
Size: 71
Fragments arranged by size:
361 71 60 39 30 30 30 21
15
SfcI C'TryA_G
Cuts at: 0 362 401 428 446 476 506 536 566
Size: 362 39 27 18 30 30 30 30
Cuts at: 566 596 657
Size: 30 61
Fragments arranged by size:
362 61 39 30 30 30 30 30
27 18
SspI AAT'ATT
Cuts at: 0 117 657
Size: 117 540
StyI C'CwwG_G
Cuts at: 0 316 657
Size: 316 341
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 110 657
Size: 110 547
Enzymes that do cut:
AleI AlwNI ApaI ApoI AvrII BaeI BanI BanII
BciVI BlpI Bme1580I BmrI Bpu10I BseRI BsgI BsmBI
Bsp1286I BstAPI BstEII BtgI EarI EcoNI EcoO109I HindIII
MmeI MslI MspA1I PasI PfoI PshAI PspOMI PstI
PvuII SfcI SspI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaLI AscI AseI
AsiSI AvaI BamHI BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII BmgBI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstXI BstYI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HpaI KasI
KpnI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI