Restriction map for
pFN21AE0463
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI BpmI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BseRI BsgI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MamI
MluI MluNI MroI MscI MspA1I
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tth111I Van91I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 1214 CGCG CG^CG
AcsI 1159 AAATTC [GA]^AATT[CT]
AflIII 633 ACACGT A^C[GA][CT]GT
AlwI 825 GGATC GGATC(4/5)
ApoI 1159 AAATTC [GA]^AATT[CT]
AvaI 1094 CCCGGG C^[CT]CG[GA]G
BanI 1108 GGCACC G^G[CT][GA]CC
BbsI 500 GAAGAC GAAGAC(2/6)
BglI 1174 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 519 AGATCT A^GATCT
BpuAI 500 GAAGAC GAAGAC(2/6)
BsaAI 634 CACGTA [CT]AC^GT[GA]
BsaI 1038 GGTCTC GGTCTC(1/5)
BsiEI 1113 CGACCG CG[GA][CT]^CG
BsmAI 1039 GTCTC GTCTC(1/5)
BsoBI 1094 CCCGGG C^[CT]CG[GA]G
BsrBI 1218 CCGCTC CCGCTC(-3/-3)
BsrFI 1203 GCCGGC [GA]^CCGG[CT]
BssSI 1120 CACGAG CACGAG(-5/-1)
BstUI 1214 CGCG CG^CG
BstYI 519 AGATCT [GA]^GATC[CT]
CfoI 1215 GCGC GCG^C
Cfr10I 1203 GCCGGC [GA]^CCGG[CT]
DraII 1097 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 970 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 336 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1097 GGGTCCC [GA]G^G[ACGT]CC[CT]
HaeIII 1182 GGCC GG^CC
HgaI 1212 GACGC GACGC(5/10)
HhaI 1215 GCGC GCG^C
HinP1I 1215 GCGC G^CGC
MfeI 772 CAATTG C^AATTG
MflI 519 AGATCT [GA]^GATC[CT]
MunI 772 CAATTG C^AATTG
MvnI 1214 CGCG CG^CG
NaeI 1203 GCCGGC GCC^GGC
NgoMIV 1203 GCCGGC G^CCGGC
NspI 807 ACATGC [GA]CATG^[CT]
PpuMI 1097 GGGTCCC [GA]G^G[AT]CC[CT]
ScaI 585 AGTACT AGT^ACT
SmaI 1094 CCCGGG CCC^GGG
SmlI 12 CTTGAG C^T[CT][GA]AG
TaqI 1005 TCGA T^CGA
TthHB8I 1005 TCGA T^CGA
XhoII 519 AGATCT [GA]^GATC[CT]
XmaCI 1094 CCCGGG C^CCGGG
XmaI 1094 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 586 GTAC GT^AC
637 GTAC
AlwNI 442 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
959 CAGACTCTG
AspHI 15 GAGCTC G[AT]GC[AT]^C
331 GAGCTC
AvaII 70 GGTCC G^G[AT]CC
1098 GGTCC
BanII 15 GAGCTC G[GA]GC[CT]^C
331 GAGCTC
BbvI 844 GCTGC GCAGC(8/12)
847 GCTGC
BmyI 15 GAGCTC G[AGT]GC[ACT]^C
331 GAGCTC
BsiHKAI 15 GAGCTC G[AT]GC[AT]^C
331 GAGCTC
BsiYI 336 CCTGGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1197 CCTGAAGCCGG
BslI 336 CCTGGAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1197 CCTGAAGCCGG
BsmFI 210 GGGAC GGGAC(10/14)
1099 GTCCC
Bsp1286I 15 GAGCTC G[AGT]GC[ACT]^C
331 GAGCTC
BsrI 232 ACTGG ACTGG(1/-1)
373 CCAGT
Cfr13I 70 GGTCC G^G[ACGT]CC
1098 GGTCC
EarI 1137 GAAGAG CTCTTC(1/4)
1240 GAAGAG
Ksp632I 1137 GAAGAG CTCTTC(1/4)
1240 GAAGAG
MaeII 635 ACGT A^CGT
720 ACGT
PleI 961 GACTC GAGTC(4/5)
1072 GAGTC
RsaI 586 GTAC GT^AC
637 GTAC
SacI 15 GAGCTC GAGCT^C
331 GAGCTC
SapI 1137 GAAGAGC GCTCTTC(1/4)
1240 GAAGAGC
Sau96I 70 GGTCC G^G[ACGT]CC
1098 GGTCC
TfiI 909 GATTC G^A[AT]TC
1002 GATTC
TseI 844 GCTGC G^C[AT]GC
847 GCTGC
Tsp45I 1118 GTCAC ^GT[GC]AC
1210 GTGAC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI BcnI BfaI BsaJI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HapII
HinfI HpaII ItaI MaeI MaeIII
MboI MboII MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV Sau3AI ScrFI SfaNI
Tru9I Tsp509I TspRI