(Circular) (Six-base) MAPSORT of: pFN21AE0463.seq Check: 810 from: 1 to: 5953 pFN21AE0463 5953 bp With 182 enzymes: SgfI * October 20, 2009 10:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3763 278 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3296 1937 Size: 1359 4594 Acc65I G'GTAC_C Cuts at: 3274 3274 Size: 5953 AclI AA'CG_TT Cuts at: 1563 4080 4453 1563 Size: 2517 373 3063 Fragments arranged by size: 3063 2517 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2592 2826 3224 4016 5400 5668 1632 Size: 960 234 398 792 1384 268 1917 Fragments arranged by size: 1917 1384 960 792 398 268 234 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5756 AflIII A'CryG_T Cuts at: 1203 2638 4852 5829 1203 Size: 1435 2214 977 1327 Fragments arranged by size: 2214 1435 1327 977 AgeI A'CCGG_T Cuts at: 4784 4784 Size: 5953 AhdI GACnn_n'nnGTC Cuts at: 4682 4682 Size: 5953 AleI CACnn'nnGTG Cuts at: 1166 3071 1166 Size: 1905 4048 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5953 AlwNI CAG_nnn'CTG Cuts at: 2452 2969 3696 5268 2452 Size: 517 727 1572 3137 Fragments arranged by size: 3137 1572 727 517 ApaLI G'TGCA_C Cuts at: 4010 5166 4010 Size: 1156 4797 ApoI r'AATT_y Cuts at: 1493 3164 3262 3488 3640 5749 1493 Size: 1671 98 226 152 2109 1697 Fragments arranged by size: 2109 1697 1671 226 152 98 AseI AT'TA_AT Cuts at: 160 1032 4507 160 Size: 872 3475 1606 Fragments arranged by size: 3475 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 AvaI C'yCGr_G Cuts at: 1870 1943 3099 3278 1870 Size: 73 1156 179 4545 Fragments arranged by size: 4545 1156 179 73 BamHI G'GATC_C Cuts at: 3283 3650 3283 Size: 367 5586 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3113 3274 4729 618 Size: 325 217 627 1326 161 1455 1842 Fragments arranged by size: 1842 1455 1326 627 325 217 161 BanII G_rGCy'C Cuts at: 1391 2024 2340 3272 1391 Size: 633 316 932 4072 Fragments arranged by size: 4072 932 633 316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2512 928 Size: 971 613 4369 Fragments arranged by size: 4369 971 613 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4144 4178 1903 Size: 34 2207 34 3678 Fragments arranged by size: 3678 2207 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3845 5055 3845 Size: 1210 4743 BclI T'GATC_A Cuts at: 1548 1548 Size: 5953 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3185 4564 136 Size: 107 122 71 631 605 1513 1379 1525 Fragments arranged by size: 1525 1513 1379 631 605 122 107 71 BglII A'GATC_T Cuts at: 2524 5948 2524 Size: 3424 2529 BlpI GC'TnA_GC Cuts at: 3370 3370 Size: 5953 Bme1580I G_kGCm'C Cuts at: 1790 4014 5170 1790 Size: 2224 1156 2573 Fragments arranged by size: 2573 2224 1156 BmgBI CAC'GTC Cuts at: 1318 2726 1318 Size: 1408 4545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2247 3684 4642 Size: 416 53 83 399 24 830 1437 958 Cuts at: 4642 442 Size: 1753 Fragments arranged by size: 1753 1437 958 830 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5953 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4613 1239 Size: 3374 2579 Bpu10I CC'TnA_GC Cuts at: 2844 2844 Size: 5953 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2038 4078 4943 5241 2038 Size: 2040 865 298 2750 Fragments arranged by size: 2750 2040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3049 4616 882 Size: 617 1550 1567 2219 Fragments arranged by size: 2219 1567 1550 617 BsaAI yAC'GTr Cuts at: 493 1204 2641 3744 493 Size: 711 1437 1103 2702 Fragments arranged by size: 2702 1437 1103 711 BsaBI GATnn'nnATC Cuts at: 5947 5947 Size: 5953 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3760 4142 4777 275 Size: 53 83 186 936 2227 382 635 1451 Fragments arranged by size: 2227 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4386 4784 5058 5205 5616 4386 Size: 398 274 147 411 4723 Fragments arranged by size: 4723 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2574 2604 1178 Size: 30 165 30 1171 30 4527 Fragments arranged by size: 4527 1171 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5953 BseYI C'CCAG_C Cuts at: 3106 5156 5652 3106 Size: 2050 496 3407 Fragments arranged by size: 3407 2050 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5380 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3403 4164 5192 1540 Size: 275 186 1120 282 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 282 275 186 BsiHKAI G_wGCw'C Cuts at: 2024 2340 4014 4099 5170 2024 Size: 316 1674 85 1071 2807 Fragments arranged by size: 2807 1674 1071 316 85 BsmI GAATG_Cn' Cuts at: 3464 3557 3464 Size: 93 5860 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2024 2340 3272 4014 4099 5170 1391 Size: 399 234 316 932 742 85 1071 2174 Fragments arranged by size: 2174 1071 932 742 399 316 234 85 BspEI T'CCGG_A Cuts at: 5616 5616 Size: 5953 BspHI T'CATG_A Cuts at: 3842 5572 3842 Size: 1730 4223 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3308 844 Size: 2464 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3225 3840 1394 Size: 429 1402 615 3507 Fragments arranged by size: 3507 1402 615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4448 4622 65 Size: 1183 3200 174 1396 Fragments arranged by size: 3200 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3208 4597 4784 1700 Size: 110 1398 1389 187 2869 Fragments arranged by size: 2869 1398 1389 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5953 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5953 BssSI C'ACGA_G Cuts at: 3125 4013 5025 3125 Size: 888 1012 4053 Fragments arranged by size: 4053 1012 888 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5953 BstBI TT'CG_AA Cuts at: 4757 4757 Size: 5953 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5953 BstYI r'GATC_y Cuts at: 1972 2524 3283 3650 4035 4052 5504 5590 5602 Size: 552 759 367 385 17 1452 86 12 Cuts at: 5602 5948 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 759 552 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5401 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5214 Fragments arranged by size: 5214 683 56 BtsI GCAGTG_nn' Cuts at: 2501 3469 4263 4283 2501 Size: 968 794 20 4171 Fragments arranged by size: 4171 968 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5953 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5918 DraI TTT'AAA Cuts at: 3257 3257 Size: 5953 DraIII CAC_nnn'GTG Cuts at: 2980 2980 Size: 5953 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4960 809 Size: 507 3644 1802 Fragments arranged by size: 3644 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3400 4289 5918 Size: 54 1425 50 275 18 1570 889 1629 Cuts at: 5918 8 Size: 43 Fragments arranged by size: 1629 1570 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3400 1537 Size: 275 1588 4090 Fragments arranged by size: 4090 1588 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3137 3240 3883 1353 Size: 1784 103 643 3423 Fragments arranged by size: 3423 1784 643 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4540 4914 5060 387 Size: 1064 138 2951 374 146 1280 Fragments arranged by size: 2951 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2022 2338 2022 Size: 316 5637 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2592 2826 3224 4016 4613 5400 5668 Size: 393 960 234 398 792 597 787 268 Cuts at: 5668 1239 Size: 1524 Fragments arranged by size: 1524 960 792 787 597 398 393 268 234 EcoNI CCTnn'n_nnAGG Cuts at: 2345 2345 Size: 5953 EcoO109I rG'GnC_Cy Cuts at: 3103 3103 Size: 5953 EcoRI G'AATT_C Cuts at: 3262 3640 3262 Size: 378 5575 FspI TGC'GCA Cuts at: 4459 4459 Size: 5953 HaeII r_GCGC'y Cuts at: 1678 5100 1678 Size: 3422 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2301 2333 4594 Size: 32 135 32 530 32 167 32 2261 Cuts at: 4594 4626 4668 4700 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2261 530 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3297 3543 669 Size: 988 281 1359 246 3079 Fragments arranged by size: 3079 1359 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5953 HpaI GTT'AAC Cuts at: 3543 3543 Size: 5953 KpnI G_GTAC'C Cuts at: 3278 3278 Size: 5953 MfeI C'AATT_G Cuts at: 2777 3552 2777 Size: 775 5178 MluI A'CGCG_T Cuts at: 5829 5829 Size: 5953 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5067 5251 1305 Size: 3762 184 2007 Fragments arranged by size: 3762 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4131 Fragments arranged by size: 4131 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2472 2883 3071 3911 4270 4429 518 Size: 648 1306 411 188 840 359 159 2042 Fragments arranged by size: 2042 1306 840 648 411 359 188 159 MspA1I CmG'CkG Cuts at: 1606 3369 4046 5194 5439 1606 Size: 1763 677 1148 245 2120 Fragments arranged by size: 2120 1763 1148 677 245 NaeI GCC'GGC Cuts at: 3210 3210 Size: 5953 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5401 NdeI CA'TA_TG Cuts at: 387 387 Size: 5953 NgoMIV G'CCGG_C Cuts at: 3208 3208 Size: 5953 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5953 NotI GC'GGCC_GC Cuts at: 3400 3400 Size: 5953 NspI r_CATG'y Cuts at: 1132 1229 2816 3311 4856 1132 Size: 97 1587 495 1545 2229 Fragments arranged by size: 2229 1587 1545 495 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5953 PciI A'CATG_T Cuts at: 4852 4852 Size: 5953 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5953 PfoI T'CCnGG_A Cuts at: 2340 3137 2340 Size: 797 5156 PmeI GTTT'AAAC Cuts at: 3257 3257 Size: 5953 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4022 4054 5535 5567 4022 Size: 32 1481 32 4408 Fragments arranged by size: 4408 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 3103 Size: 5953 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5953 PsiI TTA'TAA Cuts at: 2516 3523 2516 Size: 1007 4946 PstI C_TGCA'G Cuts at: 830 3305 830 Size: 2475 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5953 SacI G_AGCT'C Cuts at: 2024 2340 2024 Size: 316 5637 SalI G'TCGA_C Cuts at: 1936 3295 1936 Size: 1359 4594 SanDI GG'GwC_CC Cuts at: 3103 3103 Size: 5953 SapI GCTCTTCn'nnn_ Cuts at: 3137 3240 3137 Size: 103 5850 SbfI CC_TGCA'GG Cuts at: 3305 3305 Size: 5953 ScaI AGT'ACT Cuts at: 2592 4201 5913 2592 Size: 1609 1712 2632 Fragments arranged by size: 2632 1712 1609 SfcI C'TryA_G Cuts at: 826 1045 3301 4436 5117 5308 826 Size: 219 2256 1135 681 191 1471 Fragments arranged by size: 2256 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5953 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5953 SmaI CCC'GGG Cuts at: 1872 3101 3280 1872 Size: 1229 179 4545 Fragments arranged by size: 4545 1229 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2017 4057 4958 5220 820 Size: 197 926 74 2040 901 262 1553 Fragments arranged by size: 2040 1553 926 901 262 197 74 SnaBI TAC'GTA Cuts at: 493 3744 493 Size: 3251 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5953 SphI G_CATG'C Cuts at: 1132 3311 1132 Size: 2179 3774 SspI AAT'ATT Cuts at: 5 52 3877 5 Size: 47 3825 2081 Fragments arranged by size: 3825 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5953 StyI C'CwwG_G Cuts at: 513 1065 3392 513 Size: 552 2327 3074 Fragments arranged by size: 3074 2327 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3980 3997 4150 4335 4780 325 Size: 1255 2400 17 153 185 445 1498 Fragments arranged by size: 2400 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2590 4199 5911 Size: 274 80 33 51 1443 613 1609 1712 Cuts at: 5911 96 Size: 138 Fragments arranged by size: 1712 1609 1443 613 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5913 5945 5913 Size: 32 5921 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5953 XbaI T'CTAG_A Cuts at: 3289 3289 Size: 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5953 XmaI C'CCGG_G Cuts at: 1870 3099 3278 1870 Size: 1229 179 4545 Fragments arranged by size: 4545 1229 179 XmnI GAAnn'nnTTC Cuts at: 4082 4082 Size: 5953 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3761 276 Size: 53 83 186 936 2227 2468 Fragments arranged by size: 2468 2227 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI