Restriction map for
pFN21AE0459
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT14I FbaI FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I MaeI MaeII MamI
MboII MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RsaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tru9I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 370 GGATC GGATC(4/5)
AlwNI 71 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 68 CCTCAGG CC^T[ACGT]AGG
BanII 109 GGGCTC G[GA]GC[CT]^C
BmyI 109 GGGCTC G[AGT]GC[ACT]^C
BsaI 315 GAGACC GGTCTC(1/5)
BsmAI 315 GAGAC GTCTC(1/5)
BsmI 235 GAATGC GAATGC(1/-1)
Bsp1286I 109 GGGCTC G[AGT]GC[ACT]^C
BspHI 48 TCATGA T^CATGA
BspMI 116 GCAGGT ACCTGC(4/8)
BssHII 137 GCGCGC G^CGCGC
BstYI 370 GGATCT [GA]^GATC[CT]
Bsu36I 68 CCTCAGG CC^T[ACGT]AGG
DpnII 371 GATC ^GATC
DsaI 28 CCGCGG C^C[GA][CT]GG
Eco81I 68 CCTCAGG CC^T[ACGT]AGG
EcoT22I 343 ATGCAT ATGCA^T
HaeII 73 GGCGCT [GA]GCGC^[CT]
HinfI 248 GATTC G^A[ACGT]TC
KspI 28 CCGCGG CCGC^GG
MaeIII 38 GTCAC ^GT[ACGT]AC
MboI 371 GATC ^GATC
MflI 370 GGATCT [GA]^GATC[CT]
MslI 43 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 28 CCGCGG C[AC]G^C[GT]G
NdeII 371 GATC ^GATC
NsiI 343 ATGCAT ATGCA^T
NspI 345 GCATGC [GA]CATG^[CT]
PpuMI 299 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 48 TCATGA T^CATGA
SacII 28 CCGCGG CCGC^GG
Sau3AI 371 GATC ^GATC
SphI 345 GCATGC GCATG^C
TaqI 202 TCGA T^CGA
TfiI 248 GATTC G^A[AT]TC
Tsp45I 38 GTCAC ^GT[GC]AC
TthHB8I 202 TCGA T^CGA
XhoII 370 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 20 enzymes) #
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AluI 409 AGCT AG^CT
433 AGCT
BcnI 83 CCGGG CC^[GC]GG
91 CCGGG
BpmI 411 CTCCAG CTGGAG(16/14)
59 CTGGAG
BsrFI 11 GCCGGT [GA]^CCGG[CT]
437 ACCGGC
BsrI 58 ACTGG ACTGG(1/-1)
296 ACTGG
BstF5I 144 GGATG GGATG(2/0)
341 GGATG
Cfr10I 11 GCCGGT [GA]^CCGG[CT]
437 ACCGGC
DdeI 69 CTCAG C^T[ACGT]AG
313 CTGAG
DraII 299 GGGTCCC [GA]G^G[ACGT]CC[CT]
415 AGGCCCC
EcoO109I 299 GGGTCCC [GA]G^G[ACGT]CC[CT]
415 AGGCCCC
FauI 27 CCCGC CCCGC(4/6)
97 GCGGG
FokI 144 GGATG GGATG(9/13)
341 GGATG
HgaI 104 GCGTC GACGC(5/10)
452 GCGTC
HphI 378 GGTGA GGTGA(8/7)
39 TCACC
NciI 83 CCGGG CC^[GC]GG
91 CCGGG
NlaIII 49 CATG CATG^
346 CATG
PstI 114 CTGCAG CTGCA^G
445 CTGCAG
SfaNI 204 GATGC GCATC(5/9)
342 GATGC
SfcI 114 CTGCAG C^T[GA][CT]AG
445 CTGCAG
TspRI 57 CACTG CA[GC]TG[ACGT][ACGT]^
287 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AccII AciI AvaII BbvI BsaJI
BsiYI BslI BsmFI BssKI BstNI
BstUI Cac8I CfoI Cfr13I EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII ItaI MnlI MspI MvaI
MvnI MwoI NlaIV Sau96I ScrFI
TseI