Restriction map for
pFN21AE0458
(Linear) (Six-base) MAPSORT of: KIEE0458.seq Check: 2503 from: 1 to: 678
KIEE0458.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AfeI AGC'GCT
Cuts at: 0 281 678
Size: 281 397
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 28 60 124 156 458 490 678
Size: 28 32 64 32 302 32 188
Fragments arranged by size:
302 188 64 32 32 32 28
AvaI C'yCGr_G
Cuts at: 0 148 678
Size: 148 530
BanI G'GyrC_C
Cuts at: 0 520 678
Size: 520 158
BanII G_rGCy'C
Cuts at: 0 65 678
Size: 65 613
BbsI GAAGACnn'nnnn_
Cuts at: 0 408 678
Size: 408 270
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 180 214 229 263 678
Size: 180 34 15 34 415
Fragments arranged by size:
415 180 34 34 15
BlpI GC'TnA_GC
Cuts at: 0 614 678
Size: 614 64
BmrI ACTGGGnnnn_n'
Cuts at: 0 253 678
Size: 253 425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 625 678
Size: 386 239 53
Fragments arranged by size:
386 239 53
Bpu10I CC'TnA_GC
Cuts at: 0 181 678
Size: 181 497
BseYI C'CCAG_C
Cuts at: 0 441 486 590 678
Size: 441 45 104 88
Fragments arranged by size:
441 104 88 45
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 528 678
Size: 528 150
BsiHKAI G_wGCw'C
Cuts at: 0 557 678
Size: 557 121
Bsp1286I G_dGCh'C
Cuts at: 0 65 557 678
Size: 65 492 121
Fragments arranged by size:
492 121 65
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 320 678
Size: 320 358
BssHII G'CGCG_C
Cuts at: 0 160 678
Size: 160 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 678
Size: 585 93
BstXI CCAn_nnnn'nTGG
Cuts at: 0 531 678
Size: 531 147
BstYI r'GATC_y
Cuts at: 0 18 269 332 678
Size: 18 251 63 346
Fragments arranged by size:
346 251 63 18
BtgI C'CryG_G
Cuts at: 0 49 516 678
Size: 49 467 162
Fragments arranged by size:
467 162 49
DrdI GACnn_nn'nnGTC
Cuts at: 0 412 678
Size: 412 266
EarI CTCTTCn'nnn_
Cuts at: 0 82 678
Size: 82 596
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 678
Size: 19 659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 625 678
Size: 386 239 53
Fragments arranged by size:
386 239 53
EcoO109I rG'GnC_Cy
Cuts at: 0 140 491 512 678
Size: 140 351 21 166
Fragments arranged by size:
351 166 140 21
EcoRV GAT'ATC
Cuts at: 0 453 678
Size: 453 225
HaeII r_GCGC'y
Cuts at: 0 283 678
Size: 283 395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 10 22 42 54 64 96 393 425
Size: 10 12 20 12 10 32 297 32
Cuts at: 425 678
Size: 253
Fragments arranged by size:
297 253 32 32 20 12 12 10
10
HincII GTy'rAC
Cuts at: 0 639 678
Size: 639 39
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 14 522 624 678
Size: 14 508 102 54
Fragments arranged by size:
508 102 54 14
MspA1I CmG'CkG
Cuts at: 0 51 364 618 678
Size: 51 313 254 60
Fragments arranged by size:
313 254 60 51
NcoI C'CATG_G
Cuts at: 0 516 678
Size: 516 162
NspI r_CATG'y
Cuts at: 0 115 678
Size: 115 563
PpuMI rG'GwC_Cy
Cuts at: 0 140 491 678
Size: 140 351 187
Fragments arranged by size:
351 187 140
PstI C_TGCA'G
Cuts at: 0 287 678
Size: 287 391
PvuII CAG'CTG
Cuts at: 0 364 678
Size: 364 314
SacII CC_GC'GG
Cuts at: 0 52 678
Size: 52 626
SanDI GG'GwC_CC
Cuts at: 0 140 491 678
Size: 140 351 187
Fragments arranged by size:
351 187 140
SapI GCTCTTCn'nnn_
Cuts at: 0 82 678
Size: 82 596
SexAI A'CCwGG_T
Cuts at: 0 434 678
Size: 434 244
SfcI C'TryA_G
Cuts at: 0 283 645 678
Size: 283 362 33
Fragments arranged by size:
362 283 33
StyI C'CwwG_G
Cuts at: 0 516 678
Size: 516 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 235 678
Size: 235 443
Enzymes that do cut:
AfeI AloI AvaI BanI BanII BbsI BcgI BlpI
BmrI BpmI Bpu10I BseYI BsgI BsiHKAI Bsp1286I BspMI
BssHII BstAPI BstXI BstYI BtgI DrdI EarI EciI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII MmeI MspA1I
NcoI NspI PpuMI PstI PvuII SacII SanDI SapI
SexAI SfcI StyI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII EaeI EagI
EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI