(Circular) (Six-base) MAPSORT of: pFN21AE0456.seq Check: 2074 from: 1 to: 5527 pFN21AE0456 5527 bp With 182 enzymes: SgfI * October 20, 2009 10:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3337 278 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2870 1937 Size: 933 4594 Acc65I G'GTAC_C Cuts at: 2848 2848 Size: 5527 AclI AA'CG_TT Cuts at: 1563 3654 4027 1563 Size: 2091 373 3063 Fragments arranged by size: 3063 2091 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3590 4974 5242 1632 Size: 1958 1384 268 1917 Fragments arranged by size: 1958 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5330 AflIII A'CryG_T Cuts at: 1203 4426 5403 1203 Size: 3223 977 1327 Fragments arranged by size: 3223 1327 977 AgeI A'CCGG_T Cuts at: 4358 4358 Size: 5527 AhdI GACnn_n'nnGTC Cuts at: 4256 4256 Size: 5527 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5527 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5527 AlwNI CAG_nnn'CTG Cuts at: 2080 3270 4842 2080 Size: 1190 1572 2765 Fragments arranged by size: 2765 1572 1190 ApaI G_GGCC'C Cuts at: 2587 2587 Size: 5527 ApaLI G'TGCA_C Cuts at: 3584 4740 3584 Size: 1156 4371 ApoI r'AATT_y Cuts at: 1493 2836 3062 3214 5323 1493 Size: 1343 226 152 2109 1697 Fragments arranged by size: 2109 1697 1343 226 152 AseI AT'TA_AT Cuts at: 160 1032 4081 160 Size: 872 3049 1606 Fragments arranged by size: 3049 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 AvaI C'yCGr_G Cuts at: 1870 1943 2347 2852 1870 Size: 73 404 505 4545 Fragments arranged by size: 4545 505 404 73 BamHI G'GATC_C Cuts at: 2857 3224 2857 Size: 367 5160 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2652 2848 4303 618 Size: 325 217 627 865 196 1455 1842 Fragments arranged by size: 1842 1455 865 627 325 217 196 BanII G_rGCy'C Cuts at: 1391 2347 2587 2846 1391 Size: 956 240 259 4072 Fragments arranged by size: 4072 956 259 240 BbeI G_GCGC'C Cuts at: 2656 2656 Size: 5527 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4556 BbvCI CC'TCA_GC Cuts at: 2736 2736 Size: 5527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2497 2531 3718 3752 1903 Size: 34 50 34 476 34 1187 34 3678 Fragments arranged by size: 3678 1187 476 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3419 4629 3419 Size: 1210 4317 BclI T'GATC_A Cuts at: 1548 1548 Size: 5527 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2156 2661 2816 Size: 107 122 71 631 605 484 505 155 Cuts at: 2816 4138 136 Size: 1322 1525 Fragments arranged by size: 1525 1322 631 605 505 484 155 122 107 71 BglII A'GATC_T Cuts at: 5522 5522 Size: 5527 BlpI GC'TnA_GC Cuts at: 2597 2944 2597 Size: 347 5180 Bme1580I G_kGCm'C Cuts at: 1790 2253 2587 2685 3588 4744 1790 Size: 463 334 98 903 1156 2573 Fragments arranged by size: 2573 1156 903 463 334 98 BmgBI CAC'GTC Cuts at: 1318 2704 1318 Size: 1386 4141 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2650 3258 4216 Size: 416 53 83 399 24 1233 608 958 Cuts at: 4216 442 Size: 1753 Fragments arranged by size: 1753 1233 958 608 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5527 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 4187 1239 Size: 818 2130 2579 Fragments arranged by size: 2579 2130 818 Bpu10I CC'TnA_GC Cuts at: 2736 2736 Size: 5527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3652 4517 4815 3652 Size: 865 298 4364 Fragments arranged by size: 4364 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4190 882 Size: 617 2691 2219 Fragments arranged by size: 2691 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2358 3318 493 Size: 711 1154 960 2702 Fragments arranged by size: 2702 1154 960 711 BsaBI GATnn'nnATC Cuts at: 2537 5521 2537 Size: 2984 2543 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2653 3334 3716 4351 Size: 53 83 186 936 1120 681 382 635 Cuts at: 4351 275 Size: 1451 Fragments arranged by size: 1451 1120 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3960 4358 4632 4779 5190 3960 Size: 398 274 147 411 4297 Fragments arranged by size: 4297 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2037 2067 2165 2195 1178 Size: 30 165 30 634 30 98 30 4510 Fragments arranged by size: 4510 634 165 98 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2045 2114 1186 Size: 859 69 4599 Fragments arranged by size: 4599 859 69 BseYI C'CCAG_C Cuts at: 2085 2094 4730 5226 2085 Size: 9 2636 496 2386 Fragments arranged by size: 2636 2386 496 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2552 1163 Size: 573 630 186 4138 Fragments arranged by size: 4138 630 573 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2801 2977 3738 4766 1540 Size: 275 186 800 176 761 1028 2301 Fragments arranged by size: 2301 1028 800 761 275 186 176 BsiHKAI G_wGCw'C Cuts at: 2406 3588 3673 4744 2406 Size: 1182 85 1071 3189 Fragments arranged by size: 3189 1182 1071 85 BsmI GAATG_Cn' Cuts at: 3038 3131 3038 Size: 93 5434 BsmBI CGTCTCn'nnnn_ Cuts at: 2185 2185 Size: 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2253 2347 2406 2587 2685 2846 3588 Size: 399 463 94 59 181 98 161 742 Cuts at: 3588 3673 4744 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 463 399 181 161 98 94 85 59 BspEI T'CCGG_A Cuts at: 5190 5190 Size: 5527 BspHI T'CATG_A Cuts at: 3416 5146 3416 Size: 1730 3797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2882 844 Size: 1544 494 3489 Fragments arranged by size: 3489 1544 494 BsrBI CCG'CTC Cuts at: 1394 1823 2336 2793 3414 1394 Size: 429 513 457 621 3507 Fragments arranged by size: 3507 621 513 457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4022 4196 65 Size: 1183 2774 174 1396 Fragments arranged by size: 2774 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2168 2292 2660 4171 4358 1700 Size: 110 358 124 368 1511 187 2869 Fragments arranged by size: 2869 1511 368 358 187 124 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5527 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5527 BssSI C'ACGA_G Cuts at: 3587 4599 3587 Size: 1012 4515 BstAPI GCAn_nnn'nTGC Cuts at: 794 2563 794 Size: 1769 3758 BstBI TT'CG_AA Cuts at: 4331 4331 Size: 5527 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5527 BstXI CCAn_nnnn'nTGG Cuts at: 2425 2425 Size: 5527 BstYI r'GATC_y Cuts at: 1972 2532 2857 3224 3609 3626 5078 5164 5176 Size: 560 325 367 385 17 1452 86 12 Cuts at: 5176 5522 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 560 385 367 346 325 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2418 2764 513 Size: 552 1353 346 3276 Fragments arranged by size: 3276 1353 552 346 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4788 Fragments arranged by size: 4788 683 56 BtsI GCAGTG_nn' Cuts at: 2163 2561 3043 3837 3857 2163 Size: 398 482 794 20 3833 Fragments arranged by size: 3833 794 482 398 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5527 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5492 DraI TTT'AAA Cuts at: 2831 2831 Size: 5527 DraIII CAC_nnn'GTG Cuts at: 2575 2575 Size: 5527 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4534 809 Size: 507 3218 1802 Fragments arranged by size: 3218 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2022 2337 2676 Size: 54 1425 50 275 18 192 315 339 Cuts at: 2676 2688 2798 2974 3863 5492 8 Size: 12 110 176 889 1629 43 Fragments arranged by size: 1629 1425 889 339 315 275 192 176 110 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2798 2974 1537 Size: 275 986 176 4090 Fragments arranged by size: 4090 986 275 176 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2786 3457 1353 Size: 701 732 671 3423 Fragments arranged by size: 3423 732 701 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4114 4488 4634 387 Size: 1064 138 2525 374 146 1280 Fragments arranged by size: 2525 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 3590 4187 4974 5242 1239 Size: 393 425 1533 597 787 268 1524 Fragments arranged by size: 1533 1524 787 597 425 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2384 2384 Size: 5527 EcoO109I rG'GnC_Cy Cuts at: 2583 2584 2769 2583 Size: 1 185 5341 Fragments arranged by size: 5341 185 1 EcoRI G'AATT_C Cuts at: 2836 3214 2836 Size: 378 5149 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2488 2488 Size: 5527 FspI TGC'GCA Cuts at: 4033 4033 Size: 5527 HaeII r_GCGC'y Cuts at: 1678 2656 2718 4674 1678 Size: 978 62 1956 2531 Fragments arranged by size: 2531 1956 978 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2037 2069 4168 4200 4242 Size: 32 135 32 465 32 2099 32 42 Cuts at: 4242 4274 1373 Size: 32 2626 Fragments arranged by size: 2626 2099 465 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2707 2871 3117 669 Size: 988 281 769 164 246 3079 Fragments arranged by size: 3079 988 769 281 246 164 HindIII A'AGCT_T Cuts at: 748 748 Size: 5527 HpaI GTT'AAC Cuts at: 3117 3117 Size: 5527 KasI G'GCGC_C Cuts at: 2652 2652 Size: 5527 KpnI G_GTAC'C Cuts at: 2852 2852 Size: 5527 MfeI C'AATT_G Cuts at: 3126 3126 Size: 5527 MluI A'CGCG_T Cuts at: 5403 5403 Size: 5527 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4641 4825 1305 Size: 3336 184 2007 Fragments arranged by size: 3336 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2690 10 Size: 54 1425 343 858 2847 Fragments arranged by size: 2847 1425 858 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2492 3485 3844 4003 518 Size: 648 1326 993 359 159 2042 Fragments arranged by size: 2042 1326 993 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2077 2943 3620 4768 5013 1606 Size: 471 866 677 1148 245 2120 Fragments arranged by size: 2120 1148 866 677 471 245 NaeI GCC'GGC Cuts at: 2170 2662 2170 Size: 492 5035 NarI GG'CG_CC Cuts at: 2653 2653 Size: 5527 NcoI C'CATG_G Cuts at: 513 1065 2418 513 Size: 552 1353 3622 Fragments arranged by size: 3622 1353 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5527 NgoMIV G'CCGG_C Cuts at: 2168 2660 2168 Size: 492 5035 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5527 NotI GC'GGCC_GC Cuts at: 2974 2974 Size: 5527 NspI r_CATG'y Cuts at: 1132 1229 2491 2885 4430 1132 Size: 97 1262 394 1545 2229 Fragments arranged by size: 2229 1545 1262 394 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5527 PciI A'CATG_T Cuts at: 4426 4426 Size: 5527 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5527 PmeI GTTT'AAAC Cuts at: 2831 2831 Size: 5527 PmlI CAC'GTG Cuts at: 2358 2358 Size: 5527 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3596 3628 5109 5141 3596 Size: 32 1481 32 3982 Fragments arranged by size: 3982 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5527 PsiI TTA'TAA Cuts at: 3097 3097 Size: 5527 PspOMI G'GGCC_C Cuts at: 2583 2583 Size: 5527 PstI C_TGCA'G Cuts at: 830 2879 830 Size: 2049 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5527 SalI G'TCGA_C Cuts at: 1936 2869 1936 Size: 933 4594 SbfI CC_TGCA'GG Cuts at: 2879 2879 Size: 5527 ScaI AGT'ACT Cuts at: 3775 5487 3775 Size: 1712 3815 SexAI A'CCwGG_T Cuts at: 2304 2304 Size: 5527 SfcI C'TryA_G Cuts at: 826 1045 2875 4010 4691 4882 826 Size: 219 1830 1135 681 191 1471 Fragments arranged by size: 1830 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2156 2816 2156 Size: 660 4867 SfoI GGC'GCC Cuts at: 2654 2654 Size: 5527 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5527 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5527 SmaI CCC'GGG Cuts at: 1872 2349 2854 1872 Size: 477 505 4545 Fragments arranged by size: 4545 505 477 SmlI C'TyrA_G Cuts at: 820 1017 1943 3631 4532 4794 820 Size: 197 926 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3318 493 Size: 2825 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5527 SphI G_CATG'C Cuts at: 1132 2885 1132 Size: 1753 3774 SspI AAT'ATT Cuts at: 5 52 3451 5 Size: 47 3399 2081 Fragments arranged by size: 3399 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5527 StyI C'CwwG_G Cuts at: 513 1065 2418 2524 2966 513 Size: 552 1353 106 442 3074 Fragments arranged by size: 3074 1353 552 442 106 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3554 3571 3724 3909 4354 325 Size: 1255 1974 17 153 185 445 1498 Fragments arranged by size: 1974 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 2637 3773 Size: 274 80 33 51 1443 567 93 1136 Cuts at: 3773 5485 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1136 567 274 138 93 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 5487 5519 2207 Size: 32 3248 32 2215 Fragments arranged by size: 3248 2215 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2111 1316 Size: 795 4732 XbaI T'CTAG_A Cuts at: 2863 2863 Size: 5527 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5527 XmaI C'CCGG_G Cuts at: 1870 2347 2852 1870 Size: 477 505 4545 Fragments arranged by size: 4545 505 477 XmnI GAAnn'nnTTC Cuts at: 3656 3656 Size: 5527 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3335 276 Size: 53 83 186 936 1801 2468 Fragments arranged by size: 2468 1801 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SrfI SwaI XcmI