################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AviII AvrII BanII BbeI BciVI BfrI BglII BlnI BsaBI BsaHI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FseI FspI Hin1I HindIII KasI KpnI KspI MamI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AccI 1111 GTAGAC GT^[AC][GT]AC AccII 10 CGCG CG^CG Asp700I 1979 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BcgI 214 GCAACACTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1737 TGATCA T^GATCA BfaI 1009 CTAG C^TAG BsaI 955 GAGACC GGTCTC(1/5) BsmBI 1602 CGTCTC CGTCTC(1/5) BspHI 1892 TCATGA T^CATGA BsrFI 1774 GCCGGT [GA]^CCGG[CT] BstAPI 1493 GCACCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1183 GGTGACC G^GT[ACGT]ACC BstPI 1183 GGTGACC G^GT[ACGT]ACC BstUI 10 CGCG CG^CG BstXI 176 CCACCCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1774 GCCGGT [GA]^CCGG[CT] DraI 2161 TTTAAA TTT^AAA DrdI 1114 GACAGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1183 GGTGACC G^GT[ACGT]ACC FbaI 1737 TGATCA T^GATCA HaeII 918 GGCGCT [GA]GCGC^[CT] HpaI 2431 GTTAAC GTT^AAC MaeI 1009 CTAG C^TAG MfeI 1733 CAATTG C^AATTG MunI 1733 CAATTG C^AATTG MvnI 10 CGCG CG^CG NcoI 182 CCATGG C^CATGG PflMI 855 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1892 TCATGA T^CATGA Van91I 855 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 1979 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 45 enzymes) # ################################### AccIII 505 TCCGGA T^CCGGA 2138 TCCGGA AcsI 241 AAATTC [GA]^AATT[CT] 2061 GAATTT AfaI 1833 GTAC GT^AC 2298 GTAC AocI 100 CCTCAGG CC^T[ACGT]AGG 463 CCTGAGG ApoI 241 AAATTC [GA]^AATT[CT] 2061 GAATTT AspHI 1405 GTGCTC G[AT]GC[AT]^C 1761 GTGCTC AspI 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAAAGTC BamHI 468 GGATCC G^GATCC 1563 GGATCC BbrPI 345 CACGTG CAC^GTG 2196 CACGTG BbsI 1414 GTCTTC GAAGAC(2/6) 2281 GTCTTC BlpI 1596 GCTGAGC GC^T[ACGT]AGC 2173 GCTCAGC Bpu1102I 1596 GCTGAGC GC^T[ACGT]AGC 2173 GCTCAGC BpuAI 1414 GTCTTC GAAGAC(2/6) 2281 GTCTTC BsaAI 345 CACGTG [CT]AC^GT[GA] 2196 CACGTG BseAI 505 TCCGGA T^CCGGA 2138 TCCGGA BsiHKAI 1405 GTGCTC G[AT]GC[AT]^C 1761 GTGCTC BsmI 496 GAATGC GAATGC(1/-1) 31 GCATTC BspEI 505 TCCGGA T^CCGGA 2138 TCCGGA BssSI 111 CTCGTG CACGAG(-5/-1) 1726 CTCGTG BstYI 468 GGATCC [GA]^GATC[CT] 1563 GGATCC Bsu36I 100 CCTCAGG CC^T[ACGT]AGG 463 CCTGAGG CelII 1596 GCTGAGC GC^T[ACGT]AGC 2173 GCTCAGC CfoI 919 GCGC GCG^C 2045 GCGC DsaI 182 CCATGG C^C[GA][CT]GG 695 CCGTGG Eco81I 100 CCTCAGG CC^T[ACGT]AGG 463 CCTGAGG HhaI 919 GCGC GCG^C 2045 GCGC HinP1I 919 GCGC G^CGC 2045 GCGC MflI 468 GGATCC [GA]^GATC[CT] 1563 GGATCC MroI 505 TCCGGA T^CCGGA 2138 TCCGGA MseI 2162 TTAA T^TAA 2432 TTAA PflFI 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAAAGTC PmaCI 345 CACGTG CAC^GTG 2196 CACGTG PmlI 345 CACGTG CAC^GTG 2196 CACGTG PshAI 361 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1860 GACGGTGGTC RsaI 1833 GTAC GT^AC 2298 GTAC SbfI 1072 CCTGCAGG CCTGCA^GG 2271 CCTGCAGG SmaI 576 CCCGGG CCC^GGG 2234 CCCGGG SmlI 1128 CTTGAG C^T[CT][GA]AG 1875 CTCAAG Sse8387I 1072 CCTGCAGG CCTGCA^GG 2271 CCTGCAGG StuI 1026 AGGCCT AGG^CCT 1963 AGGCCT Tru9I 2162 TTAA T^TAA 2432 TTAA Tth111I 766 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC 847 GACAAAGTC XhoII 468 GGATCC [GA]^GATC[CT] 1563 GGATCC XmaCI 576 CCCGGG C^CCGGG 2234 CCCGGG XmaI 576 CCCGGG C^CCGGG 2234 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AflIII AluI AlwI AlwNI AvaI AvaII BalI BanI BbvCI BbvI BcnI BglI BmyI BpmI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI