(Linear) (Six-base) MAPSORT of: KIEE9423.seq Check: 7403 from: 1 to: 2436 KIEE9423.orf With 182 enzymes: SgfI * March 22, 2012 18:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 412 1004 2208 2436 Size: 412 592 1204 228 Fragments arranged by size: 1204 592 412 228 AccI GT'mk_AC Cuts at: 0 1112 2436 Size: 1112 1324 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1047 2436 Size: 1047 1389 AflIII A'CryG_T Cuts at: 0 344 346 2126 2436 Size: 344 2 1780 310 Fragments arranged by size: 1780 344 310 2 AleI CACnn'nnGTG Cuts at: 0 487 2436 Size: 487 1949 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1693 2436 Size: 1693 743 AlwNI CAG_nnn'CTG Cuts at: 0 83 441 861 921 2436 Size: 83 358 420 60 1515 Fragments arranged by size: 1515 420 358 83 60 ApoI r'AATT_y Cuts at: 0 241 2061 2436 Size: 241 1820 375 Fragments arranged by size: 1820 375 241 AvaI C'yCGr_G Cuts at: 0 576 1392 2234 2436 Size: 576 816 842 202 Fragments arranged by size: 842 816 576 202 BamHI G'GATC_C Cuts at: 0 468 1563 2436 Size: 468 1095 873 Fragments arranged by size: 1095 873 468 BanI G'GyrC_C Cuts at: 0 95 400 1477 2436 Size: 95 305 1077 959 Fragments arranged by size: 1077 959 305 95 BbsI GAAGACnn'nnnn_ Cuts at: 0 1407 2274 2436 Size: 1407 867 162 Fragments arranged by size: 1407 867 162 BbvCI CC'TCA_GC Cuts at: 0 1211 1348 2308 2436 Size: 1211 137 960 128 Fragments arranged by size: 1211 960 137 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 203 237 2436 Size: 203 34 2199 Fragments arranged by size: 2199 203 34 BclI T'GATC_A Cuts at: 0 1737 2436 Size: 1737 699 BglI GCCn_nnn'nGGC Cuts at: 0 446 578 725 2436 Size: 446 132 147 1711 Fragments arranged by size: 1711 446 147 132 BlpI GC'TnA_GC Cuts at: 0 1597 2174 2436 Size: 1597 577 262 Fragments arranged by size: 1597 577 262 Bme1580I G_kGCm'C Cuts at: 0 90 100 2436 Size: 90 10 2336 Fragments arranged by size: 2336 90 10 BmgBI CAC'GTC Cuts at: 0 2127 2436 Size: 2127 309 BmrI ACTGGGnnnn_n' Cuts at: 0 1351 1458 2436 Size: 1351 107 978 Fragments arranged by size: 1351 978 107 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 666 1622 2436 Size: 666 956 814 Fragments arranged by size: 956 814 666 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 833 955 973 1114 1843 1944 2376 Size: 60 773 122 18 141 729 101 432 Cuts at: 2376 2436 Size: 60 Fragments arranged by size: 773 729 432 141 122 101 60 60 18 Bpu10I CC'TnA_GC Cuts at: 0 1030 1211 1348 1516 2308 2402 2436 Size: 1030 181 137 168 792 94 34 Fragments arranged by size: 1030 792 181 168 137 94 34 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1149 1860 2436 Size: 1149 711 576 Fragments arranged by size: 1149 711 576 BsaI GGTCTCn'nnnn_ Cuts at: 0 949 2436 Size: 949 1487 BsaAI yAC'GTr Cuts at: 0 347 2198 2436 Size: 347 1851 238 Fragments arranged by size: 1851 347 238 BsaWI w'CCGG_w Cuts at: 0 505 1744 2138 2436 Size: 505 1239 394 298 Fragments arranged by size: 1239 505 394 298 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 26 1336 1366 1816 1846 2092 2122 2374 Size: 26 1310 30 450 30 246 30 252 Cuts at: 2374 2404 2436 Size: 30 32 Fragments arranged by size: 1310 450 252 246 32 30 30 30 30 26 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1573 1609 1708 2100 2296 2436 Size: 1573 36 99 392 196 140 Fragments arranged by size: 1573 392 196 140 99 36 BseYI C'CCAG_C Cuts at: 0 1929 2422 2436 Size: 1929 493 14 Fragments arranged by size: 1929 493 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 306 465 666 1120 1573 1832 2178 2436 Size: 306 159 201 454 453 259 346 258 Fragments arranged by size: 454 453 346 306 259 258 201 159 BsiHKAI G_wGCw'C Cuts at: 0 1409 1765 2436 Size: 1409 356 671 Fragments arranged by size: 1409 671 356 BsmI GAATG_Cn' Cuts at: 0 31 502 2436 Size: 31 471 1934 Fragments arranged by size: 1934 471 31 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1608 2436 Size: 1608 828 Bsp1286I G_dGCh'C Cuts at: 0 90 100 1409 1765 2436 Size: 90 10 1309 356 671 Fragments arranged by size: 1309 671 356 90 10 BspEI T'CCGG_A Cuts at: 0 505 2138 2436 Size: 505 1633 298 Fragments arranged by size: 1633 505 298 BspHI T'CATG_A Cuts at: 0 1892 2436 Size: 1892 544 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 412 439 1004 2208 2307 2436 Size: 412 27 565 1204 99 129 Fragments arranged by size: 1204 565 412 129 99 27 BsrFI r'CCGG_y Cuts at: 0 1774 2436 Size: 1774 662 BssSI C'ACGA_G Cuts at: 0 111 1726 2436 Size: 111 1615 710 Fragments arranged by size: 1615 710 111 BstAPI GCAn_nnn'nTGC Cuts at: 0 1499 2436 Size: 1499 937 BstEII G'GTnAC_C Cuts at: 0 1183 2436 Size: 1183 1253 BstXI CCAn_nnnn'nTGG Cuts at: 0 183 2436 Size: 183 2253 BstYI r'GATC_y Cuts at: 0 468 1563 2436 Size: 468 1095 873 Fragments arranged by size: 1095 873 468 Bsu36I CC'TnA_GG Cuts at: 0 101 464 2436 Size: 101 363 1972 Fragments arranged by size: 1972 363 101 BtgI C'CryG_G Cuts at: 0 182 695 2436 Size: 182 513 1741 Fragments arranged by size: 1741 513 182 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1755 2436 Size: 1755 681 BtsI GCAGTG_nn' Cuts at: 0 534 1151 1675 1844 2403 2436 Size: 534 617 524 169 559 33 Fragments arranged by size: 617 559 534 524 169 33 DraI TTT'AAA Cuts at: 0 2163 2436 Size: 2163 273 DraIII CAC_nnn'GTG Cuts at: 0 984 1755 2201 2436 Size: 984 771 446 235 Fragments arranged by size: 984 771 446 235 DrdI GACnn_nn'nnGTC Cuts at: 0 1120 2436 Size: 1120 1316 EaeI y'GGCC_r Cuts at: 0 887 986 1428 1799 1943 2436 Size: 887 99 442 371 144 493 Fragments arranged by size: 887 493 442 371 144 99 EarI CTCTTCn'nnn_ Cuts at: 0 1708 1726 1843 2257 2436 Size: 1708 18 117 414 179 Fragments arranged by size: 1708 414 179 117 18 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 60 833 955 973 1047 1114 1843 1944 Size: 60 773 122 18 74 67 729 101 Cuts at: 1944 2376 2436 Size: 432 60 Fragments arranged by size: 773 729 432 122 101 74 67 60 60 18 EcoO109I rG'GnC_Cy Cuts at: 0 599 858 939 948 1215 1827 2436 Size: 599 259 81 9 267 612 609 Fragments arranged by size: 612 609 599 267 259 81 9 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 900 2436 Size: 900 1536 HaeII r_GCGC'y Cuts at: 0 922 2436 Size: 922 1514 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 28 731 763 1122 1154 1297 1329 2436 Size: 28 703 32 359 32 143 32 1107 Fragments arranged by size: 1107 703 359 143 32 32 32 28 HincII GTy'rAC Cuts at: 0 411 1869 2433 2436 Size: 411 1458 564 3 Fragments arranged by size: 1458 564 411 3 HpaI GTT'AAC Cuts at: 0 2433 2436 Size: 2433 3 MfeI C'AATT_G Cuts at: 0 1733 2436 Size: 1733 703 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2025 2436 Size: 2025 411 MscI TGG'CCA Cuts at: 0 889 988 1430 1801 1945 2436 Size: 889 99 442 371 144 491 Fragments arranged by size: 889 491 442 371 144 99 MslI CAynn'nnrTG Cuts at: 0 181 487 665 1547 1588 1813 1973 2149 Size: 181 306 178 882 41 225 160 176 Cuts at: 2149 2436 Size: 287 Fragments arranged by size: 882 306 287 225 181 178 176 160 41 MspA1I CmG'CkG Cuts at: 0 569 710 716 1933 1991 2178 2436 Size: 569 141 6 1217 58 187 258 Fragments arranged by size: 1217 569 258 187 141 58 6 NcoI C'CATG_G Cuts at: 0 182 2436 Size: 182 2254 PasI CC'CwG_GG Cuts at: 0 943 1189 2436 Size: 943 246 1247 Fragments arranged by size: 1247 943 246 PflMI CCAn_nnn'nTGG Cuts at: 0 861 2436 Size: 861 1575 PfoI T'CCnGG_A Cuts at: 0 854 1226 2321 2436 Size: 854 372 1095 115 Fragments arranged by size: 1095 854 372 115 PmlI CAC'GTG Cuts at: 0 347 2198 2436 Size: 347 1851 238 Fragments arranged by size: 1851 347 238 PpuMI rG'GwC_Cy Cuts at: 0 599 858 948 2436 Size: 599 259 90 1488 Fragments arranged by size: 1488 599 259 90 PshAI GACnn'nnGTC Cuts at: 0 365 1864 2436 Size: 365 1499 572 Fragments arranged by size: 1499 572 365 PstI C_TGCA'G Cuts at: 0 1077 1280 1468 1688 1956 2276 2436 Size: 1077 203 188 220 268 320 160 Fragments arranged by size: 1077 320 268 220 203 188 160 PvuII CAG'CTG Cuts at: 0 710 1933 2178 2436 Size: 710 1223 245 258 Fragments arranged by size: 1223 710 258 245 SanDI GG'GwC_CC Cuts at: 0 599 2436 Size: 599 1837 SbfI CC_TGCA'GG Cuts at: 0 1077 2276 2436 Size: 1077 1199 160 Fragments arranged by size: 1199 1077 160 SfcI C'TryA_G Cuts at: 0 1073 1276 1464 1684 1952 2272 2436 Size: 1073 203 188 220 268 320 164 Fragments arranged by size: 1073 320 268 220 203 188 164 SmaI CCC'GGG Cuts at: 0 578 2236 2436 Size: 578 1658 200 Fragments arranged by size: 1658 578 200 SmlI C'TyrA_G Cuts at: 0 1128 1875 2436 Size: 1128 747 561 Fragments arranged by size: 1128 747 561 SrfI GCCC'GGGC Cuts at: 0 578 2436 Size: 578 1858 StuI AGG'CCT Cuts at: 0 1028 1965 2436 Size: 1028 937 471 Fragments arranged by size: 1028 937 471 StyI C'CwwG_G Cuts at: 0 182 379 417 2031 2436 Size: 182 197 38 1614 405 Fragments arranged by size: 1614 405 197 182 38 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 547 1354 1525 2436 Size: 547 807 171 911 Fragments arranged by size: 911 807 547 171 TatI w'GTAC_w Cuts at: 0 2297 2436 Size: 2297 139 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 97 129 1739 1771 2374 2406 2436 Size: 97 32 1610 32 603 32 30 Fragments arranged by size: 1610 603 97 32 32 32 30 Tth111I GACn'n_nGTC Cuts at: 0 769 850 2436 Size: 769 81 1586 Fragments arranged by size: 1586 769 81 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 442 996 1438 2436 Size: 442 554 442 998 Fragments arranged by size: 998 554 442 442 XmaI C'CCGG_G Cuts at: 0 576 2234 2436 Size: 576 1658 202 Fragments arranged by size: 1658 576 202 XmnI GAAnn'nnTTC Cuts at: 0 1983 2436 Size: 1983 453 Enzymes that do cut: AarI AccI AcuI AflIII AleI AlfI AlwNI ApoI AvaI BamHI BanI BbsI BbvCI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I NcoI PasI PflMI PfoI PmlI PpuMI PshAI PstI PvuII SanDI SbfI SfcI SmaI SmlI SrfI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanII BbeI BciVI BfrBI BglII BmtI BsaBI BsaHI BsiEI BsiWI BsrBI BsrDI BsrGI BssHII BstBI BstZ17I ClaI CspCI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XhoI ZraI